Band 3, serving as an anion transporter, is the most abundant protein in the humanerythrocyte plasma membrane. It has two strucurally and functionally distinct domains.The membrane domain is responsible for the anion ...Band 3, serving as an anion transporter, is the most abundant protein in the humanerythrocyte plasma membrane. It has two strucurally and functionally distinct domains.The membrane domain is responsible for the anion exchange activity, while thecytoplasmic domain of Band 3 binds with components of the cytoskeleton (such asankyrin) to stabilize the cytoskeleton network. Although the cytoplasmic domain is展开更多
The effect of lanthanum and calcium on the structure and function of human erythrocyte membranes was investigated by fluorescence polarization, spin- labeled electron spin resonance (ESR) and laser Raman spectroscopy....The effect of lanthanum and calcium on the structure and function of human erythrocyte membranes was investigated by fluorescence polarization, spin- labeled electron spin resonance (ESR) and laser Raman spectroscopy. The results showed that low concentration of La3+ (0.5 mu mol/L) activated a Little (Na++K+)-ATPase and Mg2+-ATPase activities, and it inhibited obvi ously the ATPase activities with increasing its concentrations. La3+ lowered the lipid fluidity of human erythrocyte membranes and decreased the vibration intensity of alpha-helix of the protein in the Amide I '. The effect of Ca2+ on the lipid fluidity and alpha-helix of the protein in the Amide I ' was smaller than that of La3+.展开更多
To assess the quality of human erythrocyte superoxide dismutase (SOD) injection reaching the official standard for its clinical uses Methods Human erythrocyte SOD injection prepared by McCord Fridovich's m...To assess the quality of human erythrocyte superoxide dismutase (SOD) injection reaching the official standard for its clinical uses Methods Human erythrocyte SOD injection prepared by McCord Fridovich's method without column chromatography but with some modifications was used in preclinical trials, to observe the general pharmacology and pharmacodynamics of the product Results The quality of human erythrocyte SOD injection conformed to the official standard of a biological product, which was found to be non toxic and did not have any effects on the central and autonomic nervous systems as well as cardiovascular and respiratory systems The efficacy of anti inflammation and promotion of immuno regulation especially on carrageenan and adjuvant induced polyarthritis were shown in animals Conclusion Human erythrocyte SOD injection is appropriate for prophylactic and therapeutic uses in clinical trials展开更多
The cytosolic free Ca<sup>2+</sup> in erythrocytes is around 10<sup>-6</sup> mol/L, whereas the extracellularCa<sup>2+</sup> concentration is about 10<sup>-3</sup> mol/L...The cytosolic free Ca<sup>2+</sup> in erythrocytes is around 10<sup>-6</sup> mol/L, whereas the extracellularCa<sup>2+</sup> concentration is about 10<sup>-3</sup> mol/L. Thus it results in 1000-fold transmembraneCa<sup>2+</sup> gradient across erythrocyte membrane. Our previous results have shown展开更多
基金Department of Protein Engineering, Institute of Biophysics, the Chinese Academy of Sciences, Beijing 10010, China.
文摘Band 3, serving as an anion transporter, is the most abundant protein in the humanerythrocyte plasma membrane. It has two strucurally and functionally distinct domains.The membrane domain is responsible for the anion exchange activity, while thecytoplasmic domain of Band 3 binds with components of the cytoskeleton (such asankyrin) to stabilize the cytoskeleton network. Although the cytoplasmic domain is
文摘The effect of lanthanum and calcium on the structure and function of human erythrocyte membranes was investigated by fluorescence polarization, spin- labeled electron spin resonance (ESR) and laser Raman spectroscopy. The results showed that low concentration of La3+ (0.5 mu mol/L) activated a Little (Na++K+)-ATPase and Mg2+-ATPase activities, and it inhibited obvi ously the ATPase activities with increasing its concentrations. La3+ lowered the lipid fluidity of human erythrocyte membranes and decreased the vibration intensity of alpha-helix of the protein in the Amide I '. The effect of Ca2+ on the lipid fluidity and alpha-helix of the protein in the Amide I ' was smaller than that of La3+.
文摘To assess the quality of human erythrocyte superoxide dismutase (SOD) injection reaching the official standard for its clinical uses Methods Human erythrocyte SOD injection prepared by McCord Fridovich's method without column chromatography but with some modifications was used in preclinical trials, to observe the general pharmacology and pharmacodynamics of the product Results The quality of human erythrocyte SOD injection conformed to the official standard of a biological product, which was found to be non toxic and did not have any effects on the central and autonomic nervous systems as well as cardiovascular and respiratory systems The efficacy of anti inflammation and promotion of immuno regulation especially on carrageenan and adjuvant induced polyarthritis were shown in animals Conclusion Human erythrocyte SOD injection is appropriate for prophylactic and therapeutic uses in clinical trials
文摘The cytosolic free Ca<sup>2+</sup> in erythrocytes is around 10<sup>-6</sup> mol/L, whereas the extracellularCa<sup>2+</sup> concentration is about 10<sup>-3</sup> mol/L. Thus it results in 1000-fold transmembraneCa<sup>2+</sup> gradient across erythrocyte membrane. Our previous results have shown
