AIM: To assess human cytomegalovirus-encoded US28 gene function in colorectal cancer(CRC) pathogenesis.METHODS: Immunohistochemical analysis was performed to determine US28 expression in 103 CRC patient samples and 98...AIM: To assess human cytomegalovirus-encoded US28 gene function in colorectal cancer(CRC) pathogenesis.METHODS: Immunohistochemical analysis was performed to determine US28 expression in 103 CRC patient samples and 98 corresponding adjacent noncancerous samples. Patient data were compared by age, sex, tumor location, histological grade, Dukes' stage, and overall mean survival time. In addition, the US28 gene was transiently transfected into the CRC LOVO cell line, and cell proliferation was assessed using a cell counting kit-8 assay. Cell cycle analysis by flow cytometry and a cell invasion transwell assay were also carried out.RESULTS: US28 levels were clearly higher in CRC tissues(38.8%) than in adjacent noncancerous samples(7.1%)(P = 0.000). Interestingly, elevated US28 amounts in CRC tissues were significantly associated with histological grade, metastasis, Dukes' stage, and overall survival(all P < 0.05); meanwhile, US28 expression was not significantly correlated with age, sex or tumor location. In addition, multivariate Coxregression data revealed US28 level as an independent CRC prognostic marker(P = 0.000). LOVO cells successfully transfected with the US28 gene exhibited higher viability, greater chemotherapy resistance, accelerated cell cycle progression, and increased invasion ability.CONCLUSION: US28 expression is predictive of poor prognosis and may promote CRC.展开更多
Objective To investigate the expression of Calbindin-d28k （CaBP-d28k） in human endometrium. Methods Thirty-three samples of human normal endometrial tissues were divided into 6 groups： early proliferative stage （n...Objective To investigate the expression of Calbindin-d28k （CaBP-d28k） in human endometrium. Methods Thirty-three samples of human normal endometrial tissues were divided into 6 groups： early proliferative stage （n =6）, mid proliferative stage （n =5）, late proliferative stage （n=5）, early secretory stage （n=7）, mid secretory stage （n=5） and late secretory stage （n=5）. The expression and change of CaBP-d28k protein and gene were determined by immunohistochemistry and reverse transcription polymerase chain reaction methods. Results In endometrial samples, the expression of CaBP-d28k protein was mainly observed in the cytoplasm of luminal and glandular epithelium. In the menstrual cycle, the level of CaBP-d28k protein in the epithelium was the lowest during the early and mid proliferative stages, and was the highest during the mid secretory stage, then decreased in the late secretory stage （P〈0.05）. In the stroma, the expressed type of CaBP-d28k protein was the same as in the epithelium, but was lower than that in the epithelium（P〈0.05）. The CaBP-d28k mRNA was at the lowest level in the early proliferative stage（P〈0.05）, and significantly increased in the late proliferative, and early, mid secretory stages （P〈0. 05）. Conclusion Both CaBP-d28k protein and gene were expressed in human endometrium, and their expression had cyclic changes.展开更多
Generally, TGF-βs are held to down- regulate the growth of immune cells and to inhibit the development of certain differentiated functions, such as the induction of LAK activity by IL-2. In the present study, the eff...Generally, TGF-βs are held to down- regulate the growth of immune cells and to inhibit the development of certain differentiated functions, such as the induction of LAK activity by IL-2. In the present study, the effects of TGF-β1 on the proliferation and cytotoxicity of human PBMC activated by anti-CD3 or and-CD3 plus anti-CD28 was investigated. The results demonstrated that TGF- β1 clearly inhibits the induction of cytotoxic ability in human PBMC stimulated via CD3 or CD3 and CD28 ( P<0. 01) , without significantly altering the proliferative response to these stimuli, at the tested doses of TGF-β1. Co-stimulation with IL-2 was hardly altered, suggesting that TGF-β1 action is affected by the nature of the costimulatory signals.展开更多
AIM:To test whether the status of positive cytomegalovirus(CMV) DNA detection adds to the predictive value of IL28B and to further categorize C/T allele carriers.METHODS:This study included 166 chronic hepatitis C(CHC...AIM:To test whether the status of positive cytomegalovirus(CMV) DNA detection adds to the predictive value of IL28B and to further categorize C/T allele carriers.METHODS:This study included 166 chronic hepatitis C(CHC) patients who received combined interferon and ribavirin therapy for 48 wk,84 spontaneous hepatitis C virus(HCV) resolvers who were positive for IgG anti-HCV antibody and negative for HCV RNA,and 100 healthy subjects who were negative for both HCV antibodies and RNA as controls.Genomic DNA from peripheral blood was used for IL28B rs.12979860 single nucleotide polymorphism(SNP) and CMV DNA detection.A 139 bp fragment containing IL28B SNP was amplified in all subjects by polymerase chain reaction using a specifically designed primer.Then the IL28B rs.12979860 SNP was detected by restriction fragment length polymorphism(RFLP) genotyping.The presence of CMV DNA was tested by amplification of the gB1 gene using nested polymerase chain reaction.The role of CMV and IL28B rs.12979860 SNP genotypes in determining the response rate to combined interferon therapy and clinical status of patients were statistically analyzed.RESULTS:Current data showed that 67% of patients carrying the IL28B 12979860 C/C allele had a sustained viral response(SVR) while the genotypes C/T and TT were associated with lower SVR rates,50% and 48%,respectively.SVR rates for the C/C allele were lower than other HCV genotypes and/or other populations.Genotype CC was associated with the response to interferon(P = 0.025).Genotype C/C was reduced from 48% in controls to 14% in CHC patients suggesting its protective role against progression to chronicity.The majority of spontaneously cleared subjects(86%) were C/C,confirming its protective role.The C/T allele was present in 71% of CHC patients compared with 38% of controls,so the use of IL28B SNP genotyping only in these patients may be of little value as a predictor of response.CMV reactivation occurred in 40% of CHC patients.Co-infection with CMV seriously diminished the response to interferon(IFN) therapy,with SVR rates in C/C genotypes 87.5% in CMV-negative patients and 12.5% in CMV-positive patients(P < 0.0001).SVR rates among C/T carriers were reduced to < 50% in patients with positive CMV DNA while the non-response rate doubled.These data indicate that a supplemental assay for CMV viremia adds to the prognostic value of IL28B genotyping.CONCLUSION:The results suggest that both genetic(i.e.,spontaneous) and therapeutic(IFN-based therapy) arms are complementary in the battle against HCV.CMV DNA testing may be of value to better predict the response to IFN,particularly in IL28B C/T carriers.展开更多
目的汉化并改良护士认知工作复杂性测评工具(Complexity Assessment and Monitoring to Ensure Optimal Outcomes,CAMEOⅡ),验证其应用于我国成人ICU护理工作量评估中的有效性。方法研究团队在征得原作者同意后对CAMEOⅡ进行汉化与文化...目的汉化并改良护士认知工作复杂性测评工具(Complexity Assessment and Monitoring to Ensure Optimal Outcomes,CAMEOⅡ),验证其应用于我国成人ICU护理工作量评估中的有效性。方法研究团队在征得原作者同意后对CAMEOⅡ进行汉化与文化调试,通过专家咨询及预试验,最终形成16个维度、149个条目的中文版CAMEOⅡ。于2020年11—12月,在上海交通大学附属第一人民医院ICU内,由经过培训的10名护士分别运用中文版CAMEOⅡ与治疗干预评分系统(Therapeutic Intervention Scoring System-28,Tiss-28)对日班和夜班的护理工作量进行评估,比较这2种评估工具对护理人力资源需求评估的相关性。结果中文版CAMEOⅡ形成过程中,2轮德尔菲法的专家积极系数均为100%,专家权威系数为0.885,量表各条目的变异系数范围在0~0.097,肯德尔和谐系数为0.860,Cronbach’sα系数为0.798,量表各条目的内容效度指数为0.890~1.000,平均内容效度指数为0.960。在日班评估中,中文版CAMEOⅡ及Tiss-28的评估结果呈正相关(r=0.437,P<0.01);夜班评估中,中文版CAMEOⅡ及Tiss-28的评估结果呈正相关(r=0.296,P<0.01)。结论中文版CAMEOⅡ具有一定的实用性,能为我国ICU护理人力资源评估及配置提供参考。展开更多
Objective To investigate expression and localization of Calbindin-d28k (CaBP-d28k) in human ovary. Methods The expression of the CaBP-d28k protein and gene in 23 human ovarian samples was measured by immunohistochemis...Objective To investigate expression and localization of Calbindin-d28k (CaBP-d28k) in human ovary. Methods The expression of the CaBP-d28k protein and gene in 23 human ovarian samples was measured by immunohistochemistry and reverse transcription polymerase chain reaction (RTPCR) methods. Results Positive expression of the CaBP-d28k protein and mRNA was observed in human ovary. The ovarian expression of the CaBP-d28k protein appeared in the cytoplasm of oocytes, granulose cells, theca cells, and two types of cells of corpus luteum. From primordial follicle, primary follicle to secondary follicle, increased CaBP- d28k levels were showed (P〈0. 05), and the CaBP-d28k expression in secondary follicle had the highest level. Conclusion Human ovarian tissue locally expresses CaBP-d28k, and its expression level increases with follicular development.展开更多
基金Supported by The Zhejiang Provincial Natural Science Foundation of China,No.LY15H160059Zhejiang Provincial Medical and Health Science and Technology Project,No.2016KYB192the Wenzhou Municipal Science and Technology Bureau,No.Y20140691
文摘AIM: To assess human cytomegalovirus-encoded US28 gene function in colorectal cancer(CRC) pathogenesis.METHODS: Immunohistochemical analysis was performed to determine US28 expression in 103 CRC patient samples and 98 corresponding adjacent noncancerous samples. Patient data were compared by age, sex, tumor location, histological grade, Dukes' stage, and overall mean survival time. In addition, the US28 gene was transiently transfected into the CRC LOVO cell line, and cell proliferation was assessed using a cell counting kit-8 assay. Cell cycle analysis by flow cytometry and a cell invasion transwell assay were also carried out.RESULTS: US28 levels were clearly higher in CRC tissues(38.8%) than in adjacent noncancerous samples(7.1%)(P = 0.000). Interestingly, elevated US28 amounts in CRC tissues were significantly associated with histological grade, metastasis, Dukes' stage, and overall survival(all P < 0.05); meanwhile, US28 expression was not significantly correlated with age, sex or tumor location. In addition, multivariate Coxregression data revealed US28 level as an independent CRC prognostic marker(P = 0.000). LOVO cells successfully transfected with the US28 gene exhibited higher viability, greater chemotherapy resistance, accelerated cell cycle progression, and increased invasion ability.CONCLUSION: US28 expression is predictive of poor prognosis and may promote CRC.
文摘Objective To investigate the expression of Calbindin-d28k （CaBP-d28k） in human endometrium. Methods Thirty-three samples of human normal endometrial tissues were divided into 6 groups： early proliferative stage （n =6）, mid proliferative stage （n =5）, late proliferative stage （n=5）, early secretory stage （n=7）, mid secretory stage （n=5） and late secretory stage （n=5）. The expression and change of CaBP-d28k protein and gene were determined by immunohistochemistry and reverse transcription polymerase chain reaction methods. Results In endometrial samples, the expression of CaBP-d28k protein was mainly observed in the cytoplasm of luminal and glandular epithelium. In the menstrual cycle, the level of CaBP-d28k protein in the epithelium was the lowest during the early and mid proliferative stages, and was the highest during the mid secretory stage, then decreased in the late secretory stage （P〈0.05）. In the stroma, the expressed type of CaBP-d28k protein was the same as in the epithelium, but was lower than that in the epithelium（P〈0.05）. The CaBP-d28k mRNA was at the lowest level in the early proliferative stage（P〈0.05）, and significantly increased in the late proliferative, and early, mid secretory stages （P〈0. 05）. Conclusion Both CaBP-d28k protein and gene were expressed in human endometrium, and their expression had cyclic changes.
文摘Generally, TGF-βs are held to down- regulate the growth of immune cells and to inhibit the development of certain differentiated functions, such as the induction of LAK activity by IL-2. In the present study, the effects of TGF-β1 on the proliferation and cytotoxicity of human PBMC activated by anti-CD3 or and-CD3 plus anti-CD28 was investigated. The results demonstrated that TGF- β1 clearly inhibits the induction of cytotoxic ability in human PBMC stimulated via CD3 or CD3 and CD28 ( P<0. 01) , without significantly altering the proliferative response to these stimuli, at the tested doses of TGF-β1. Co-stimulation with IL-2 was hardly altered, suggesting that TGF-β1 action is affected by the nature of the costimulatory signals.
基金Supported by Misr El-Khair Foundation,Cairo,Egypt
文摘AIM:To test whether the status of positive cytomegalovirus(CMV) DNA detection adds to the predictive value of IL28B and to further categorize C/T allele carriers.METHODS:This study included 166 chronic hepatitis C(CHC) patients who received combined interferon and ribavirin therapy for 48 wk,84 spontaneous hepatitis C virus(HCV) resolvers who were positive for IgG anti-HCV antibody and negative for HCV RNA,and 100 healthy subjects who were negative for both HCV antibodies and RNA as controls.Genomic DNA from peripheral blood was used for IL28B rs.12979860 single nucleotide polymorphism(SNP) and CMV DNA detection.A 139 bp fragment containing IL28B SNP was amplified in all subjects by polymerase chain reaction using a specifically designed primer.Then the IL28B rs.12979860 SNP was detected by restriction fragment length polymorphism(RFLP) genotyping.The presence of CMV DNA was tested by amplification of the gB1 gene using nested polymerase chain reaction.The role of CMV and IL28B rs.12979860 SNP genotypes in determining the response rate to combined interferon therapy and clinical status of patients were statistically analyzed.RESULTS:Current data showed that 67% of patients carrying the IL28B 12979860 C/C allele had a sustained viral response(SVR) while the genotypes C/T and TT were associated with lower SVR rates,50% and 48%,respectively.SVR rates for the C/C allele were lower than other HCV genotypes and/or other populations.Genotype CC was associated with the response to interferon(P = 0.025).Genotype C/C was reduced from 48% in controls to 14% in CHC patients suggesting its protective role against progression to chronicity.The majority of spontaneously cleared subjects(86%) were C/C,confirming its protective role.The C/T allele was present in 71% of CHC patients compared with 38% of controls,so the use of IL28B SNP genotyping only in these patients may be of little value as a predictor of response.CMV reactivation occurred in 40% of CHC patients.Co-infection with CMV seriously diminished the response to interferon(IFN) therapy,with SVR rates in C/C genotypes 87.5% in CMV-negative patients and 12.5% in CMV-positive patients(P < 0.0001).SVR rates among C/T carriers were reduced to < 50% in patients with positive CMV DNA while the non-response rate doubled.These data indicate that a supplemental assay for CMV viremia adds to the prognostic value of IL28B genotyping.CONCLUSION:The results suggest that both genetic(i.e.,spontaneous) and therapeutic(IFN-based therapy) arms are complementary in the battle against HCV.CMV DNA testing may be of value to better predict the response to IFN,particularly in IL28B C/T carriers.
文摘Objective To investigate expression and localization of Calbindin-d28k (CaBP-d28k) in human ovary. Methods The expression of the CaBP-d28k protein and gene in 23 human ovarian samples was measured by immunohistochemistry and reverse transcription polymerase chain reaction (RTPCR) methods. Results Positive expression of the CaBP-d28k protein and mRNA was observed in human ovary. The ovarian expression of the CaBP-d28k protein appeared in the cytoplasm of oocytes, granulose cells, theca cells, and two types of cells of corpus luteum. From primordial follicle, primary follicle to secondary follicle, increased CaBP- d28k levels were showed (P〈0. 05), and the CaBP-d28k expression in secondary follicle had the highest level. Conclusion Human ovarian tissue locally expresses CaBP-d28k, and its expression level increases with follicular development.
