AIM： To assess the associations of human leukocyte antigen （HI_A） class Ⅱ DQB1＊0301 and/or DRB1＊1101 allele with spontaneous hepatitis C virus （HCV） clearance by meta-analysis of individual dataset from all st...AIM： To assess the associations of human leukocyte antigen （HI_A） class Ⅱ DQB1＊0301 and/or DRB1＊1101 allele with spontaneous hepatitis C virus （HCV） clearance by meta-analysis of individual dataset from all studies published till date. METHODS： To clarify the impact of HLA class Ⅱ polymorphisms on viral clearance, we performed a metaanalysis of the published data from 11 studies comparing the frequencies of DQB1＊0301 and DRB1＊1101 alleles in individuals with spontaneous resolution to those with persistent infection. As we identified the heterogeneity between studies, summary statistical data were calculated based on a random-effect model. RESULTS： Meta-analyses yielded summary estimatesodds ratio （OR） of 2.36 [95%CI （1.62, 3.43）, P〈0.00001] and 2.02 [95%CI （1.56, 2.62）, P〈0.00001] for the effects of DQB1＊0301 and DRB1＊1101 alleles on spontaneous clearance of HCV, respectively. CONCLUSION： These results support the hypothesis that specific HLA class Ⅱ alleles might influence the susceptibility or resistance to persistent HCV infection. Both DQB1＊0301 and DRB1＊1101 are protective alleles and present HCV epitopes more effectively to CD4^＋T lymphocytes than others, and subjects with these two alleles are at a lower risk of developing chronic HCV infection. Large, multi-ethnic confirmatory and welldesigned studies are needed to determine the host genetic determinants of HCV infection.展开更多
AIM:To explore whether predisposition to autoimmune gastritis (AIG) is found in human leukocyte antigen (HLA), cytokine or killer cell immunoglobulin-like receptor (KIR) gene variations.METHODS: Twelve Finnish patient...AIM:To explore whether predisposition to autoimmune gastritis (AIG) is found in human leukocyte antigen (HLA), cytokine or killer cell immunoglobulin-like receptor (KIR) gene variations.METHODS: Twelve Finnish patients with autoimmunetype severe atrophy of the gastric corpus were included. The patients' serum was analyzed for pepsinogen-interleukin (IL)-1 gene cluster, IL-2, IL-4, IL-6, IL-10, IL-12, interferon γ, transforming growth factor β, and tumor necrosis factor α. Variation in KIR genes was also explored. The results were compared with prevalence of the polymorphisms in Finnish or European populations.RESULTS: All patients had pepsinogen-CONCLUSION: As explored with modern DNA-based methods, HLA-DRB1*04 and DQB1*03 alleles, but not HLA-B8-DRB1*03, may predispose to AIG.展开更多
To investigate the HLA-A, -B allele polymorphism in Han population of Shandong province and to explore the possibility to find out the HLA-A,-B-matehed cord blood donors for stem cell transplantation to be used in oth...To investigate the HLA-A, -B allele polymorphism in Han population of Shandong province and to explore the possibility to find out the HLA-A,-B-matehed cord blood donors for stem cell transplantation to be used in other area in China, 5844 umbilical cord blood samples were taken from Han population donors of Shandong province, and assayed with PCR-sequence-oligonucleotide （PCR-SSO） assay. In Shandong Hart donors, 20 alleles at HLA-A locus and 46 alleles at HLA-B locus could be detected as revealed in the present study. Among the 20 alleles at HLA-A locus, the most prevalent five alleles included A ＊ 02（0.3041）, A ＊ 11（0. 1443）, A ＊ 24（0. 1434）, A ＊ 30（0.0975） and A 33（0.0859）, while, the alleles with lower gene frequencies included A ＊ 34（0.0006）, A ＊ 25 （0.0005）, A ＊ 66（0.0005）, A ＊ 74（0.0004） and A ＊ （0.0001）. Of the 46 HLA-B alleles detected, the most prevalent five alleles were B ＊ 13（0.1348）, B ＊ 51（0.0713）, B ＊ 62（0.0712）, B ＊ 61 （0.0676） and B ＊ 60（0.0642）; while alleles with lower gene frequencies included B ＊ 77（0.0001）, B ＊ 76（0.0002）, B ＊ 47（0.0003）, B ＊ 42（0.0003） and B ＊ 72（0.0004）. In comparison with those of the other Han population in China, the HLA-A, -B gene frequencies in the umbilical cord blood of Shandong province possess unique distribution features among the investigated populations from various regions of the same race origin, and the differences in various regions of the same race were less than those among the different race. It is evident that the HLA-A,-B alleles of the umbilical cord blood taken in Shangdong province show high degree of polymorphism, and it might be part of those of Northem Han population in China. So, it is reasonable for patients of Northern Chinese to receive HLA class Ⅰ -match transplant of cord blood stem ceils for tissue and organ transplantation from Shangdong umbilical cord blood bank.展开更多
[目的]从HLA-DR、DQ基因角度探讨三氯乙烯职业中毒皮肤损害易感的遗传背景。[方法]采用PCR- SSP(sequence specific primer)法对三氯乙烯职业中毒皮肤损害组(病例组)及对照组进行HLA-DR、DQ基因位点等位基因分型。[结果]HLA-DRB1位点在...[目的]从HLA-DR、DQ基因角度探讨三氯乙烯职业中毒皮肤损害易感的遗传背景。[方法]采用PCR- SSP(sequence specific primer)法对三氯乙烯职业中毒皮肤损害组(病例组)及对照组进行HLA-DR、DQ基因位点等位基因分型。[结果]HLA-DRB1位点在病例组检出11个等位基因,11个血清型;对照组检出13个等位基因,13个血清型。HLA-DQB1位点在病例组及对照组均检出5个等位基因,7个血清型。获得了各等位基因分布及基因频率资料,其中DRB1*09(DR9)和DQB1*03(DQ9)等位基因频率在病例组与对照组间差异均有显著性(P<0．05);OR值(95％可信区间)分别为DRB1*09(DR9)等位基因为0．174(0．037～0．830),DQB1*03(DQ9)等位基因为0．226(0．046～1．108)。[结论] DRB1*09(DR9)和DQB1*03(DQ9)等位基因可能是三氯乙烯职业中毒皮肤损害的保护基因。展开更多
基金Supported by the National Natural Science Foundation of China,No. 30200232
文摘AIM： To assess the associations of human leukocyte antigen （HI_A） class Ⅱ DQB1＊0301 and/or DRB1＊1101 allele with spontaneous hepatitis C virus （HCV） clearance by meta-analysis of individual dataset from all studies published till date. METHODS： To clarify the impact of HLA class Ⅱ polymorphisms on viral clearance, we performed a metaanalysis of the published data from 11 studies comparing the frequencies of DQB1＊0301 and DRB1＊1101 alleles in individuals with spontaneous resolution to those with persistent infection. As we identified the heterogeneity between studies, summary statistical data were calculated based on a random-effect model. RESULTS： Meta-analyses yielded summary estimatesodds ratio （OR） of 2.36 [95%CI （1.62, 3.43）, P〈0.00001] and 2.02 [95%CI （1.56, 2.62）, P〈0.00001] for the effects of DQB1＊0301 and DRB1＊1101 alleles on spontaneous clearance of HCV, respectively. CONCLUSION： These results support the hypothesis that specific HLA class Ⅱ alleles might influence the susceptibility or resistance to persistent HCV infection. Both DQB1＊0301 and DRB1＊1101 are protective alleles and present HCV epitopes more effectively to CD4^＋T lymphocytes than others, and subjects with these two alleles are at a lower risk of developing chronic HCV infection. Large, multi-ethnic confirmatory and welldesigned studies are needed to determine the host genetic determinants of HCV infection.
基金Supported by A grant from the Research Unit of the Health Centre,City of Helsinki
文摘AIM:To explore whether predisposition to autoimmune gastritis (AIG) is found in human leukocyte antigen (HLA), cytokine or killer cell immunoglobulin-like receptor (KIR) gene variations.METHODS: Twelve Finnish patients with autoimmunetype severe atrophy of the gastric corpus were included. The patients' serum was analyzed for pepsinogen-interleukin (IL)-1 gene cluster, IL-2, IL-4, IL-6, IL-10, IL-12, interferon γ, transforming growth factor β, and tumor necrosis factor α. Variation in KIR genes was also explored. The results were compared with prevalence of the polymorphisms in Finnish or European populations.RESULTS: All patients had pepsinogen-CONCLUSION: As explored with modern DNA-based methods, HLA-DRB1*04 and DQB1*03 alleles, but not HLA-B8-DRB1*03, may predispose to AIG.
文摘To investigate the HLA-A, -B allele polymorphism in Han population of Shandong province and to explore the possibility to find out the HLA-A,-B-matehed cord blood donors for stem cell transplantation to be used in other area in China, 5844 umbilical cord blood samples were taken from Han population donors of Shandong province, and assayed with PCR-sequence-oligonucleotide （PCR-SSO） assay. In Shandong Hart donors, 20 alleles at HLA-A locus and 46 alleles at HLA-B locus could be detected as revealed in the present study. Among the 20 alleles at HLA-A locus, the most prevalent five alleles included A ＊ 02（0.3041）, A ＊ 11（0. 1443）, A ＊ 24（0. 1434）, A ＊ 30（0.0975） and A 33（0.0859）, while, the alleles with lower gene frequencies included A ＊ 34（0.0006）, A ＊ 25 （0.0005）, A ＊ 66（0.0005）, A ＊ 74（0.0004） and A ＊ （0.0001）. Of the 46 HLA-B alleles detected, the most prevalent five alleles were B ＊ 13（0.1348）, B ＊ 51（0.0713）, B ＊ 62（0.0712）, B ＊ 61 （0.0676） and B ＊ 60（0.0642）; while alleles with lower gene frequencies included B ＊ 77（0.0001）, B ＊ 76（0.0002）, B ＊ 47（0.0003）, B ＊ 42（0.0003） and B ＊ 72（0.0004）. In comparison with those of the other Han population in China, the HLA-A, -B gene frequencies in the umbilical cord blood of Shandong province possess unique distribution features among the investigated populations from various regions of the same race origin, and the differences in various regions of the same race were less than those among the different race. It is evident that the HLA-A,-B alleles of the umbilical cord blood taken in Shangdong province show high degree of polymorphism, and it might be part of those of Northem Han population in China. So, it is reasonable for patients of Northern Chinese to receive HLA class Ⅰ -match transplant of cord blood stem ceils for tissue and organ transplantation from Shangdong umbilical cord blood bank.
文摘[目的]从HLA-DR、DQ基因角度探讨三氯乙烯职业中毒皮肤损害易感的遗传背景。[方法]采用PCR- SSP(sequence specific primer)法对三氯乙烯职业中毒皮肤损害组(病例组)及对照组进行HLA-DR、DQ基因位点等位基因分型。[结果]HLA-DRB1位点在病例组检出11个等位基因,11个血清型;对照组检出13个等位基因,13个血清型。HLA-DQB1位点在病例组及对照组均检出5个等位基因,7个血清型。获得了各等位基因分布及基因频率资料,其中DRB1*09(DR9)和DQB1*03(DQ9)等位基因频率在病例组与对照组间差异均有显著性(P<0．05);OR值(95％可信区间)分别为DRB1*09(DR9)等位基因为0．174(0．037～0．830),DQB1*03(DQ9)等位基因为0．226(0．046～1．108)。[结论] DRB1*09(DR9)和DQB1*03(DQ9)等位基因可能是三氯乙烯职业中毒皮肤损害的保护基因。
