We present our approach for constructing a federated searching system for Japanese humanities digital libraries using automatic metadata mapping.We discuss some achievements in the ongoing research to construct a fede...We present our approach for constructing a federated searching system for Japanese humanities digital libraries using automatic metadata mapping.We discuss some achievements in the ongoing research to construct a federated searching system. The goals of this system are(1) to perform metadata mapping automatically for Japanese heterogeneous humanities digital libraries and(2) to let users access multiple humanities digital libraries by using only one query input.The proposed automatic metadata mapping method produced the average mapping precision of 94.9%.We also address the metadata-related challenges facing Japanese humanities databases.展开更多
Yeast two-hybrid approach is popularly used nowadays as an important technical method in the field of studying protein-protein interactions.Although yeast two-hybrid system is obviously advantageous in searching inter...Yeast two-hybrid approach is popularly used nowadays as an important technical method in the field of studying protein-protein interactions.Although yeast two-hybrid system is obviously advantageous in searching interacting proteins and setting up the network of proteins interaction,not all of proteins can use routine yeast two-hybrid method to search interacting proteins.Many important proteins,such as some nucleoprotein transcriptional factor,carry out the regular method and construct the bait-BD vector to screen the library containing AD vector.However,it usually results in failures because it contains the activate domain and can self-activate the reporter gene.In this study,we changed the research strategy,fused the bait gene(FOXA3)with the AD vector to screen the library containing BD vector,so that we constructed a two-hybrid library containing BD vector and can bypass the interference of self-activation.And we used this two-hybrid library to screen FOXA3,a hepatocyte nuclear factor,and found out an interacting protein:complement component C3.展开更多
基金Project supported by the Grant-in-Aid for the Global COE Program from the Ministry of Education,Culture,Sports,Science and Technology (MEXT) of Japan,and MEXT Grant-in-Aid for Strategic Formation of Research Infrastructure for Private University,Japan
文摘We present our approach for constructing a federated searching system for Japanese humanities digital libraries using automatic metadata mapping.We discuss some achievements in the ongoing research to construct a federated searching system. The goals of this system are(1) to perform metadata mapping automatically for Japanese heterogeneous humanities digital libraries and(2) to let users access multiple humanities digital libraries by using only one query input.The proposed automatic metadata mapping method produced the average mapping precision of 94.9%.We also address the metadata-related challenges facing Japanese humanities databases.
基金supported by Chinese National key Grant of Basic Research (2001 CB510203)Hi-Tech Research and Development Program of China (2002AA,227011 and 2002BA711A11)+1 种基金CNHLPP (2004BA711A19)Shanghai Science and Technology Innovation Program.
文摘Yeast two-hybrid approach is popularly used nowadays as an important technical method in the field of studying protein-protein interactions.Although yeast two-hybrid system is obviously advantageous in searching interacting proteins and setting up the network of proteins interaction,not all of proteins can use routine yeast two-hybrid method to search interacting proteins.Many important proteins,such as some nucleoprotein transcriptional factor,carry out the regular method and construct the bait-BD vector to screen the library containing AD vector.However,it usually results in failures because it contains the activate domain and can self-activate the reporter gene.In this study,we changed the research strategy,fused the bait gene(FOXA3)with the AD vector to screen the library containing BD vector,so that we constructed a two-hybrid library containing BD vector and can bypass the interference of self-activation.And we used this two-hybrid library to screen FOXA3,a hepatocyte nuclear factor,and found out an interacting protein:complement component C3.
