Objective: To investigate the effect of insulin-like growth factor-1 (IGF-1) on pituitary tumor transforming gene (PTTG) in glioma C6 cells. Methods: Glioma C6 cells were divided into four groups: A group, treated wit...Objective: To investigate the effect of insulin-like growth factor-1 (IGF-1) on pituitary tumor transforming gene (PTTG) in glioma C6 cells. Methods: Glioma C6 cells were divided into four groups: A group, treated without IGF-1; B group, treated with 0.1 ng/mL dose of IGF-1; C group, treated with 1 ng/mL dose of IGF-1; D group, treated with 10 ng/mL dose of IGF-1. PTTG mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR), western blotting was used to detect the expression of PTTG protein. Results: The expressions of PTTG mRNA were 1.370 ± 0.212, 2.198 ± 0.354, 3.452 ± 0.332, and 4.576 ± 0.387 respectively in the four groups, and there was a significantly difference between any two groups (P < 0.01). The protein expressions of PTTG in the four groups were 1.407 ± 0.334, 1.813 ± 0.465, 2.412 ± 0.576, and 3.128 ± 0.665 respectively, and there was a significantly difference between any two groups (P < 0.01). Conclusion: IGF-1 can up-regulate the expression of PTTG significantly in dosage-dependent manner.展开更多
Objective:To study the change of pituitary tumor transforming gene 1 (PTTG1) expression in prostate cancer and its correlation with proliferation and invasion genes.Methods: Patients with prostate cancer who underwent...Objective:To study the change of pituitary tumor transforming gene 1 (PTTG1) expression in prostate cancer and its correlation with proliferation and invasion genes.Methods: Patients with prostate cancer who underwent radical operation in our hospital between March 2015 and January 2018 were selected as the malignant group of the research, and the prostate cancer lesions were collected;patients who underwent transurethral resection of the prostate due to benign prostatic hyperplasia in our hospital during the same period were selected as the benign group of the research, and the benign prostate lesions were collected. The mRNA expression levels of PTTG1, proliferation genes and invasion genes in the lesions were determined. Results:PTTG1, Survivin, Bcl-2, CyclinD1, GPRC6A, ZEB1, CatB, CatD and PAR-1 mRNA expression in prostate cancer lesions of malignant group were significantly higher than those of benign group whereas CDKN2, p21 and TFPI2 mRNA expression were significantly lower than those of benign group;Survivin, Bcl-2, CyclinD1, GPRC6A, ZEB1, CatB, CatD and PAR-1 mRNA expression in prostate cancer lesions with high PTTG1 were significantly higher than those in prostate cancer lesions with low PTTG1 whereas CDKN2, p21 and TFPI2 mRNA expression were significantly lower than those in prostate cancer lesions with low PTTG1.Conclusion:The PTTG1 gene is highly expressed in prostate cancer lesions and it is closely related to the changes of proliferation and invasion gene expression.展开更多
目的研究人垂体瘤转化基因1(hum an p itu itary tumor transform ing gene 1,PTTG1)上调表达对肺腺癌细胞株(A549)增殖和凋亡的影响。方法构建含PTTG1基因的真核表达载体,脂质体法转染A549细胞并用G418筛选出高表达的阳性克隆株,实时...目的研究人垂体瘤转化基因1(hum an p itu itary tumor transform ing gene 1,PTTG1)上调表达对肺腺癌细胞株(A549)增殖和凋亡的影响。方法构建含PTTG1基因的真核表达载体,脂质体法转染A549细胞并用G418筛选出高表达的阳性克隆株,实时荧光定量PCR(real-tim e fluorescent quantitation PCR)和免疫细胞化学(immunocytochem ical assay,ICA)分别检测PTTG1 mRNA及蛋白表达的改变,3H胸腺嘧啶核甙(3H-TdR)掺入法检测细胞增殖活性,AnnexinⅤ异硫氢酸荧光素(FITC)试剂盒检测凋亡情况。结果成功构建含PTTG1基因的真核表达载体,获得上调表达PTTG1的A549细胞,转染含PTTG1基因重组质粒的A549细胞增殖活性较未转染组细胞及转染空白质粒组细胞均显著增强(P<0.05);3组细胞间的凋亡率无显著差异。结论PTTG1基因能显著促进A549细胞的增殖,而对细胞凋亡没有影响。展开更多
基金the grant of Natural Science Foundation of College and University of Jiangsu Province (No. 05KJD320238).
文摘Objective: To investigate the effect of insulin-like growth factor-1 (IGF-1) on pituitary tumor transforming gene (PTTG) in glioma C6 cells. Methods: Glioma C6 cells were divided into four groups: A group, treated without IGF-1; B group, treated with 0.1 ng/mL dose of IGF-1; C group, treated with 1 ng/mL dose of IGF-1; D group, treated with 10 ng/mL dose of IGF-1. PTTG mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR), western blotting was used to detect the expression of PTTG protein. Results: The expressions of PTTG mRNA were 1.370 ± 0.212, 2.198 ± 0.354, 3.452 ± 0.332, and 4.576 ± 0.387 respectively in the four groups, and there was a significantly difference between any two groups (P < 0.01). The protein expressions of PTTG in the four groups were 1.407 ± 0.334, 1.813 ± 0.465, 2.412 ± 0.576, and 3.128 ± 0.665 respectively, and there was a significantly difference between any two groups (P < 0.01). Conclusion: IGF-1 can up-regulate the expression of PTTG significantly in dosage-dependent manner.
基金National Natural Science Youth Science Foundation No:81300626.
文摘Objective:To study the change of pituitary tumor transforming gene 1 (PTTG1) expression in prostate cancer and its correlation with proliferation and invasion genes.Methods: Patients with prostate cancer who underwent radical operation in our hospital between March 2015 and January 2018 were selected as the malignant group of the research, and the prostate cancer lesions were collected;patients who underwent transurethral resection of the prostate due to benign prostatic hyperplasia in our hospital during the same period were selected as the benign group of the research, and the benign prostate lesions were collected. The mRNA expression levels of PTTG1, proliferation genes and invasion genes in the lesions were determined. Results:PTTG1, Survivin, Bcl-2, CyclinD1, GPRC6A, ZEB1, CatB, CatD and PAR-1 mRNA expression in prostate cancer lesions of malignant group were significantly higher than those of benign group whereas CDKN2, p21 and TFPI2 mRNA expression were significantly lower than those of benign group;Survivin, Bcl-2, CyclinD1, GPRC6A, ZEB1, CatB, CatD and PAR-1 mRNA expression in prostate cancer lesions with high PTTG1 were significantly higher than those in prostate cancer lesions with low PTTG1 whereas CDKN2, p21 and TFPI2 mRNA expression were significantly lower than those in prostate cancer lesions with low PTTG1.Conclusion:The PTTG1 gene is highly expressed in prostate cancer lesions and it is closely related to the changes of proliferation and invasion gene expression.
文摘目的研究人垂体瘤转化基因1(hum an p itu itary tumor transform ing gene 1,PTTG1)上调表达对肺腺癌细胞株(A549)增殖和凋亡的影响。方法构建含PTTG1基因的真核表达载体,脂质体法转染A549细胞并用G418筛选出高表达的阳性克隆株,实时荧光定量PCR(real-tim e fluorescent quantitation PCR)和免疫细胞化学(immunocytochem ical assay,ICA)分别检测PTTG1 mRNA及蛋白表达的改变,3H胸腺嘧啶核甙(3H-TdR)掺入法检测细胞增殖活性,AnnexinⅤ异硫氢酸荧光素(FITC)试剂盒检测凋亡情况。结果成功构建含PTTG1基因的真核表达载体,获得上调表达PTTG1的A549细胞,转染含PTTG1基因重组质粒的A549细胞增殖活性较未转染组细胞及转染空白质粒组细胞均显著增强(P<0.05);3组细胞间的凋亡率无显著差异。结论PTTG1基因能显著促进A549细胞的增殖,而对细胞凋亡没有影响。
