The cell lines PGbE1 and PGLH7, which have high and low metastatic potential respectively, were two different variants isolated from human pulmonary giant cell carcinoma cell line PG. This study compared the expressi...The cell lines PGbE1 and PGLH7, which have high and low metastatic potential respectively, were two different variants isolated from human pulmonary giant cell carcinoma cell line PG. This study compared the expression and response of several growth factors TGFa, TGFb, bFGF, IL 6, IL 8 and ANG in the two cell lines. By using RT PCR analysis and thymidine incor poration assay, it was found that IL 6, TGFa and their receptors IL 6R and EGFR were expressed at higher level in PGbE1 cells than in PGLH7 cells, while no significant differences were found in the expression of ANG, bFGF, IL 8, IL 8R and TGFβ. Recombinant IL 6 and TGFα stimulated the proliferation of both cells, while TGFβ had dual effects. These results suggest that ANG, bFGF IL 6, IL 8 and TGFα, β may be involved in the proliferation of pulmonary giant cell carcinoma via autocrine mechanism, and IL 6 and TGFa may play an important role in the metastasis of tumor cells.展开更多
文摘The cell lines PGbE1 and PGLH7, which have high and low metastatic potential respectively, were two different variants isolated from human pulmonary giant cell carcinoma cell line PG. This study compared the expression and response of several growth factors TGFa, TGFb, bFGF, IL 6, IL 8 and ANG in the two cell lines. By using RT PCR analysis and thymidine incor poration assay, it was found that IL 6, TGFa and their receptors IL 6R and EGFR were expressed at higher level in PGbE1 cells than in PGLH7 cells, while no significant differences were found in the expression of ANG, bFGF, IL 8, IL 8R and TGFβ. Recombinant IL 6 and TGFα stimulated the proliferation of both cells, while TGFβ had dual effects. These results suggest that ANG, bFGF IL 6, IL 8 and TGFα, β may be involved in the proliferation of pulmonary giant cell carcinoma via autocrine mechanism, and IL 6 and TGFa may play an important role in the metastasis of tumor cells.
