Human thioredoxin and antibacterial peptide, PR39, have been shown to have potent antioxidant effects that may prolong survival of cells during hypoxia. The pSSCMV/human thioredoxin-PR39 vector was successfully constr...Human thioredoxin and antibacterial peptide, PR39, have been shown to have potent antioxidant effects that may prolong survival of cells during hypoxia. The pSSCMV/human thioredoxin-PR39 vector was successfully constructed in this study and used to infect ECV304 cells. Transfected ECV304 cells were incubated at 1%, 5% hypoxic, and normal oxygen conditions. We found that the number of apoptotic cells after transfection with recombinant adeno-associated virus-human thioredoxin -PR39 was significantly lower than controls, suggesting a protective effect of the recombinant human thioredoxin-PR39 protein on hypoxic cells.展开更多
An alternative splice variant of human thioredoxin (hTRX) has been identified by using reverse transcription_polymerase chain reaction, cloning and expressing of hTRX_encoding gene, and DNA sequencing. This variant co...An alternative splice variant of human thioredoxin (hTRX) has been identified by using reverse transcription_polymerase chain reaction, cloning and expressing of hTRX_encoding gene, and DNA sequencing. This variant contains hTRX exons 1, 2, 4 and 5. The entire protein encoding region of this variant, named hTRXδ3, is identical to hTRX except for the omission of exon 3. Human TRX and TRXδ3 cDNA has been expressed in \%E.coli\%. The catalytic activity of rhTRXδ3 on DTT_dependent insulin reduction is lower than that of rhTRX, although hTRXδ3 still contains the active site.展开更多
目的探讨腹腔积液中人硫氧还蛋白(human thioredoxin,hTRX)过表达与小儿腹膜间皮细胞炎症的关联。方法实验设计分4组:对照组(B-Z组),rhTRX高剂量组(G-Y组),rhTRX中剂量组(Z-Y组),rhTRXl低剂量组(L-Y组);采用MTT比色法检测小儿腹膜间皮...目的探讨腹腔积液中人硫氧还蛋白(human thioredoxin,hTRX)过表达与小儿腹膜间皮细胞炎症的关联。方法实验设计分4组:对照组(B-Z组),rhTRX高剂量组(G-Y组),rhTRX中剂量组(Z-Y组),rhTRXl低剂量组(L-Y组);采用MTT比色法检测小儿腹膜间皮细胞的存活率;用试剂盒进行T淋巴细胞亚群的测定;测定4组小儿腹膜间皮细胞培养上清液中炎性因子IFN-γ的浓度;采用RT-PCR和酶联免疫吸附法测定各组中的干扰素-γ(IFN-γ)基因和蛋白的表达水平。结果与B-Z组相比,L-Y组、Z-Y组和G-Y组IFN-γ浓度下降( P <0.05),随rhTRX剂量的增加,小儿腹膜间皮细胞的存活率升高( P <0.05);RT-PCR和ELISA结果显示L-Y组中IFN-γ的表达水平无明显下降,Z-Y组和G-Y组细胞的IFN-γ表达水平与B-Z组相比有明显下降( P <0.05)。结论 腹腔积液中hTRX过表达对小儿腹膜间皮细胞炎症具有抑制作用。展开更多
基金sponsored by the National Natural Science Foundation of China,No.30970992
文摘Human thioredoxin and antibacterial peptide, PR39, have been shown to have potent antioxidant effects that may prolong survival of cells during hypoxia. The pSSCMV/human thioredoxin-PR39 vector was successfully constructed in this study and used to infect ECV304 cells. Transfected ECV304 cells were incubated at 1%, 5% hypoxic, and normal oxygen conditions. We found that the number of apoptotic cells after transfection with recombinant adeno-associated virus-human thioredoxin -PR39 was significantly lower than controls, suggesting a protective effect of the recombinant human thioredoxin-PR39 protein on hypoxic cells.
文摘An alternative splice variant of human thioredoxin (hTRX) has been identified by using reverse transcription_polymerase chain reaction, cloning and expressing of hTRX_encoding gene, and DNA sequencing. This variant contains hTRX exons 1, 2, 4 and 5. The entire protein encoding region of this variant, named hTRXδ3, is identical to hTRX except for the omission of exon 3. Human TRX and TRXδ3 cDNA has been expressed in \%E.coli\%. The catalytic activity of rhTRXδ3 on DTT_dependent insulin reduction is lower than that of rhTRX, although hTRXδ3 still contains the active site.
文摘目的探讨腹腔积液中人硫氧还蛋白(human thioredoxin,hTRX)过表达与小儿腹膜间皮细胞炎症的关联。方法实验设计分4组:对照组(B-Z组),rhTRX高剂量组(G-Y组),rhTRX中剂量组(Z-Y组),rhTRXl低剂量组(L-Y组);采用MTT比色法检测小儿腹膜间皮细胞的存活率;用试剂盒进行T淋巴细胞亚群的测定;测定4组小儿腹膜间皮细胞培养上清液中炎性因子IFN-γ的浓度;采用RT-PCR和酶联免疫吸附法测定各组中的干扰素-γ(IFN-γ)基因和蛋白的表达水平。结果与B-Z组相比,L-Y组、Z-Y组和G-Y组IFN-γ浓度下降( P <0.05),随rhTRX剂量的增加,小儿腹膜间皮细胞的存活率升高( P <0.05);RT-PCR和ELISA结果显示L-Y组中IFN-γ的表达水平无明显下降,Z-Y组和G-Y组细胞的IFN-γ表达水平与B-Z组相比有明显下降( P <0.05)。结论 腹腔积液中hTRX过表达对小儿腹膜间皮细胞炎症具有抑制作用。
