<abstract>Aim: To identify possible spermicidal agents through screening a number of edible medicinal plants with antimicrobial activity. Methods: Initial screening was made on the basis of ram cauda epididymal ...<abstract>Aim: To identify possible spermicidal agents through screening a number of edible medicinal plants with antimicrobial activity. Methods: Initial screening was made on the basis of ram cauda epididymal sperm immobilization immediately after addition of extracts. The most potent extract was selected and was evaluated on both ram and human spermatozoa. To unravel its mode of action several sperm functional tests were carried out, namely viability of cells, hypo-osmotic swelling test for membrane integrity and assays of membrane-bound enzyme 5'-nucleotidase and acrosomal marker enzyme acrosin. Results: The crude aqueous extract of the bulb of Allium sativum L. showed the most promising results by instant immobilization of the ram epididymal sperm at 0.25 g/mL and human ejaculated sperm at 0.5 g/mL. Sperm immobilizing effects were irreversible and the factor of the extract responsible for immobilization was thermostable up to 90 癈. On boiling at 100 癈 for 10 minutes, this activity was markedly reduced. Moreover, this extract was able to cause aggregation of ram sperms into small clusters after 30 minutes of incubation at 37 癈. However this property was not found in human spermatozoa. More than 50 % reduction in sperm viability and hypo-osmotic swelling occurred in treated sperm as compared with the controls, indicating the possibility of plasma membrane disintegration which was further supported by the significant reduction in the activity of membrane bound 5'-nucleotidase and acrosomal acrosin. Conclusion: The crude aqueous extract of A. sativum bulb possesses spermicidal activity in vitro.展开更多
Lithium's(Li)ubiquitous distribution in the environment is a rising concern due to its rapid proliferation in the modern electronic industry.Li enigmatic entry into the terrestrial food chain raises many questions...Lithium's(Li)ubiquitous distribution in the environment is a rising concern due to its rapid proliferation in the modern electronic industry.Li enigmatic entry into the terrestrial food chain raises many questions and uncertainties that may pose a grave threat to living biota.We examined the leverage existing published articles regarding advances in global Li resources,interplay with plants,and possible involvement with living organisms,especially humans and animals.Globally,Li concentration(<10 e300 mg kg1)is detected in agricultural soil,and their pollutant levels vary with space and time.High mobility of Li results in higher accumulation in plants,but the clear mechanisms and specific functions remain unknown.Our assessment reveals the causal relationship between Li level and biota health.For example,lower Li intake(<0.6 mM in serum)leads to mental disorders,while higher intake(>1.5 mM in serum)induces thyroid,stomach,kidney,and reproductive system dysfunctions in humans and animals.However,there is a serious knowledge gap regarding Li regulatory standards in environmental compartments,and mechanistic approaches to unveil its consequences are needed.Furthermore,aggressive efforts are required to define optimum levels of Li for the normal functioning of animals,plants,and humans.This review is designed to revitalize the current status of Li research and identify the key knowledge gaps to fight back against the mountainous challenges of Li during the recent digital revolution.Additionally,we propose pathways to overcome Li problems and develop a strategy for effective,safe,and acceptable applications.展开更多
Asian Pacific Journal of Reproduction(APJR)is a biomonthly international journal which serves researchers and clinicians in the fields of human,animal and plant reproduction.It publishes high quality original research...Asian Pacific Journal of Reproduction(APJR)is a biomonthly international journal which serves researchers and clinicians in the fields of human,animal and plant reproduction.It publishes high quality original research and topical reviews on the subject of reproductive sciences.We encourage manuscripts on cellular and molecular mechanisms of reproduction,fertility and infertility,gynecologic and reproductive oncology,embryology,obstetrics,gynecology,andrologjs maternal-fetal medicine,reproductive endocrinology,reproductive immunology,reproductive genetics,and genetic breeding.In addition,we support new and emerging topics in more applied areas of reproduction,including assisted reproductive technologies,prenatal and preimplantation genetic diagnosis,cloning,stem cell research,and genetic breeding.Descriptive papers are welcome provided they shed some light on reproductive science.Original scientific researches,timely reviews,case reports,short communications,and letters to the editor related to the research and practice of reproduction are encouraged.展开更多
Neuritis of lateral cutaneous nerve of thigh, also called meralgia paresthetica, is a commonly encountered disease in clinical practice. The author has treated 28 cases of meralgia paresthetica since 1996 by acupunctu...Neuritis of lateral cutaneous nerve of thigh, also called meralgia paresthetica, is a commonly encountered disease in clinical practice. The author has treated 28 cases of meralgia paresthetica since 1996 by acupuncture plus point-injection with satisfactory results. A report follows.Clinical Data Among the 28 cases, 13 were male and 15 female. Their age ranged from 24 to 72 years, most of the patients being 35 to 50 years; the duration of illness varied from one month to ten years and 6 cases were affected on left side, 8 cases on right side and 14 cases on both sides.展开更多
In this paper, the landscape design of the Bailuxi Wetland in University of Sanya was studied by using the research method of “survey—analysis—design”. The questionnaire survey, field observation and landscape pat...In this paper, the landscape design of the Bailuxi Wetland in University of Sanya was studied by using the research method of “survey—analysis—design”. The questionnaire survey, field observation and landscape pattern analysis were used to analyze the landscape pattern of the Bailuxi Wetland as well as the characteristics and problems of the six subsystems of green space, activity, road, animal, plant, and sound. Then the overall structure and function design of the Bailuxi Wetland landscape, the optimization adjustment of the landscape pattern, the optimization design of the subsystem, and the relationship design between human and the animal, plant, and sound systems were proposed. This study is dedicated to providing effective advice on the planning and design of campus wetland landscapes, with a view to providing examples for the planning and design of campus wetland landscapes. The research indicates that:① Scientific and rational spatial structure and functional layout planning are prerequisites for the sustainable development of wetland landscapes;② the balanced development of subsystems in the campus wetland system is a necessary condition for maintaining the rationality of the campus landscape pattern;③ the degree of harmony between humans and animals, plants, and sounds affects the ecological sustainability of campus wetland landscapes.展开更多
文摘<abstract>Aim: To identify possible spermicidal agents through screening a number of edible medicinal plants with antimicrobial activity. Methods: Initial screening was made on the basis of ram cauda epididymal sperm immobilization immediately after addition of extracts. The most potent extract was selected and was evaluated on both ram and human spermatozoa. To unravel its mode of action several sperm functional tests were carried out, namely viability of cells, hypo-osmotic swelling test for membrane integrity and assays of membrane-bound enzyme 5'-nucleotidase and acrosomal marker enzyme acrosin. Results: The crude aqueous extract of the bulb of Allium sativum L. showed the most promising results by instant immobilization of the ram epididymal sperm at 0.25 g/mL and human ejaculated sperm at 0.5 g/mL. Sperm immobilizing effects were irreversible and the factor of the extract responsible for immobilization was thermostable up to 90 癈. On boiling at 100 癈 for 10 minutes, this activity was markedly reduced. Moreover, this extract was able to cause aggregation of ram sperms into small clusters after 30 minutes of incubation at 37 癈. However this property was not found in human spermatozoa. More than 50 % reduction in sperm viability and hypo-osmotic swelling occurred in treated sperm as compared with the controls, indicating the possibility of plasma membrane disintegration which was further supported by the significant reduction in the activity of membrane bound 5'-nucleotidase and acrosomal acrosin. Conclusion: The crude aqueous extract of A. sativum bulb possesses spermicidal activity in vitro.
基金funded by the The 111 project of the Education Ministry of China(B18053)The National Natural Science Foundation(32130081).
文摘Lithium's(Li)ubiquitous distribution in the environment is a rising concern due to its rapid proliferation in the modern electronic industry.Li enigmatic entry into the terrestrial food chain raises many questions and uncertainties that may pose a grave threat to living biota.We examined the leverage existing published articles regarding advances in global Li resources,interplay with plants,and possible involvement with living organisms,especially humans and animals.Globally,Li concentration(<10 e300 mg kg1)is detected in agricultural soil,and their pollutant levels vary with space and time.High mobility of Li results in higher accumulation in plants,but the clear mechanisms and specific functions remain unknown.Our assessment reveals the causal relationship between Li level and biota health.For example,lower Li intake(<0.6 mM in serum)leads to mental disorders,while higher intake(>1.5 mM in serum)induces thyroid,stomach,kidney,and reproductive system dysfunctions in humans and animals.However,there is a serious knowledge gap regarding Li regulatory standards in environmental compartments,and mechanistic approaches to unveil its consequences are needed.Furthermore,aggressive efforts are required to define optimum levels of Li for the normal functioning of animals,plants,and humans.This review is designed to revitalize the current status of Li research and identify the key knowledge gaps to fight back against the mountainous challenges of Li during the recent digital revolution.Additionally,we propose pathways to overcome Li problems and develop a strategy for effective,safe,and acceptable applications.
文摘Asian Pacific Journal of Reproduction(APJR)is a biomonthly international journal which serves researchers and clinicians in the fields of human,animal and plant reproduction.It publishes high quality original research and topical reviews on the subject of reproductive sciences.We encourage manuscripts on cellular and molecular mechanisms of reproduction,fertility and infertility,gynecologic and reproductive oncology,embryology,obstetrics,gynecology,andrologjs maternal-fetal medicine,reproductive endocrinology,reproductive immunology,reproductive genetics,and genetic breeding.In addition,we support new and emerging topics in more applied areas of reproduction,including assisted reproductive technologies,prenatal and preimplantation genetic diagnosis,cloning,stem cell research,and genetic breeding.Descriptive papers are welcome provided they shed some light on reproductive science.Original scientific researches,timely reviews,case reports,short communications,and letters to the editor related to the research and practice of reproduction are encouraged.
文摘Neuritis of lateral cutaneous nerve of thigh, also called meralgia paresthetica, is a commonly encountered disease in clinical practice. The author has treated 28 cases of meralgia paresthetica since 1996 by acupuncture plus point-injection with satisfactory results. A report follows.Clinical Data Among the 28 cases, 13 were male and 15 female. Their age ranged from 24 to 72 years, most of the patients being 35 to 50 years; the duration of illness varied from one month to ten years and 6 cases were affected on left side, 8 cases on right side and 14 cases on both sides.
基金Sponsored by Hainan Natural Science Fund(417179)the Key Project of Teaching Reform Research in Colleges and Universities in Hainan Province(HNJG2019ZD-24)the National Undergraduate Innovation and Entrepreneurship Training Program(201813892560)
文摘In this paper, the landscape design of the Bailuxi Wetland in University of Sanya was studied by using the research method of “survey—analysis—design”. The questionnaire survey, field observation and landscape pattern analysis were used to analyze the landscape pattern of the Bailuxi Wetland as well as the characteristics and problems of the six subsystems of green space, activity, road, animal, plant, and sound. Then the overall structure and function design of the Bailuxi Wetland landscape, the optimization adjustment of the landscape pattern, the optimization design of the subsystem, and the relationship design between human and the animal, plant, and sound systems were proposed. This study is dedicated to providing effective advice on the planning and design of campus wetland landscapes, with a view to providing examples for the planning and design of campus wetland landscapes. The research indicates that:① Scientific and rational spatial structure and functional layout planning are prerequisites for the sustainable development of wetland landscapes;② the balanced development of subsystems in the campus wetland system is a necessary condition for maintaining the rationality of the campus landscape pattern;③ the degree of harmony between humans and animals, plants, and sounds affects the ecological sustainability of campus wetland landscapes.
