期刊文献+
共找到26篇文章
< 1 2 >
每页显示 20 50 100
BETWEEN HONOR AND HUMILIATION: STARTING FROM THE RIGHTS AND WRONGS BY FOREIGN INVESTMENT (Ⅱ)
1
作者 Ma Yu 《大经贸》 北大核心 1997年第5期82-86,共5页
Money Scatters Everywhere in China It is said that the saving ratio of China is close to 40%.Authoritative persons thus analyze in this way: theoretically, 40% saving ratio of a country is enough tosustain its economi... Money Scatters Everywhere in China It is said that the saving ratio of China is close to 40%.Authoritative persons thus analyze in this way: theoretically, 40% saving ratio of a country is enough tosustain its economic growth at the rate over 10% even at thetime of its economic take-off; practically, "Four Tigers" inAsia achieved high economic growth in 1960s and 1970s withtheir saving ratio only about 30%. Conclusion: with the 40% saving ratio, or domesticfunds, China can fully sustain the growth rate of 8-9 % thisyear. The question remains: why do we want to introduceforeign funds? Why do we allow foreigners to come and maketens percent of profit while we can only get bits of 展开更多
关键词 BETWEEN HONOR AND humiliation STARTING FROM THE RIGHTS AND WRONGS BY FOREIGN INVESTMENT
下载PDF
BETWEEN HONOR AND HUMILIATION:——STARTING FROM THE RIGHTS AND WRONGS BY FOREIGN INVESTMENT(Ⅲ)
2
作者 Ma Yu 《大经贸》 北大核心 1997年第6期90-92,共3页
The Poor Becomes Even Poorer, And TheRich Becomes Even Richer-The Mat-thew Effect In Foreign Investmenthe nature of the capital is keen on the rich but cold-shoul-der the poor. If you are rich, dollars, pounds or RMBs... The Poor Becomes Even Poorer, And TheRich Becomes Even Richer-The Mat-thew Effect In Foreign Investmenthe nature of the capital is keen on the rich but cold-shoul-der the poor. If you are rich, dollars, pounds or RMBs wouldgreet you with a genial smile. If you are poor, you will beignored. Maybe, you will be provided with some "grants", to showtheir generosity and mercy. Nevertheless, with regard to invest-ment, not a chance.Therefore, foreign investment in China takes the followingshape:by regions, the realized foreign investment in the 18 prov-inces and autonomous regions of the Mid-West accounts for lessthan 10% of the national total even though the area is in greatneed of funds; by industrial sectors, agriculture has only a tinyshare of less than 2% of total foreign investment in China eventhough it cniovs first Prioritv in economic development and de-mands immediate strengthening; likewise, foreign investment 展开更多
关键词 STARTING FROM THE RIGHTS AND WRONGS BY FOREIGN INVESTMENT BETWEEN HONOR AND humiliation
下载PDF
Eliminating a Century-oldNational Humiliation
3
《China Today》 1997年第7期7-7,共1页
关键词 In Eliminating a Century-oldNational humiliation
下载PDF
ChMYB1-ChbHLH42-ChTTG1 module regulates abscisic acidinduced anthocyanin biosynthesis in Cerasus humilis
4
作者 Xiaolong Ji Zhe Li +2 位作者 Mingyu Zhang Shaoyu Lang Xingshun Song 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第1期51-65,共15页
Cerasus humilis is a kind of economic fruit tree peculiar to China,which is widely used in the food,landscape,and pharmaceutical industries.Anthocyanins are a phenolic metabolite that plays an essential role in fruit ... Cerasus humilis is a kind of economic fruit tree peculiar to China,which is widely used in the food,landscape,and pharmaceutical industries.Anthocyanins are a phenolic metabolite that plays an essential role in fruit coloration.However,the regulatory network of C.humilis in anthocyanin biosynthesis is still unclear.In this study,the R2R3-MYB transcription factor ChMYB1 was isolated from the full genome of the species.Yeast one-hybrid,dual-luciferase assays,and GUS staining showed that ChMYB1 significantly increased anthocyanin contents in C.humilis fruit by promoting the expression of ChCHS and ChUFGT by binding MBS(MYB-binding elements).ChMYB1 interacted with ChbHLH42and ChTTG1 to form the MBW complex and further enhanced the expression of ChUFGT.In addition,abscisic acid(ABA)treatment promoted the expression of ChMYB1 and anthocyanin accumulation in C.humilis fruit.Interestingly,ABA treatment enhanced the interaction between ChMYB1 and ChbHLH42.Furthermore,ChABI5 inhibited the interaction between ChMYB1 and ChbHLH42.Our data elucidated the primary molecular mechanism of anthocyanin biosynthesis in C.humilis fruit,deepening the understanding of the regulatory network affecting anthocyanin metabolism in edible fruit crops. 展开更多
关键词 Cerasus humilis ANTHOCYANIN ChMYB1 FRUIT ABA
下载PDF
Selection of Suitable Area and Analysis of Adaptability Difference of Prunus humilis
5
作者 Ze YAO Shengxiu JIANG +2 位作者 Zizhu YAN Qi WANG Dawei WANG 《Asian Agricultural Research》 2024年第3期41-45,共5页
This paper delves into and assesses the differences in biological traits and fruit nutritional composition of 3 varieties of Prunus humilis introduced from 2 different regions.Results showed that(i)the contents of pro... This paper delves into and assesses the differences in biological traits and fruit nutritional composition of 3 varieties of Prunus humilis introduced from 2 different regions.Results showed that(i)the contents of protein,total sugar,and calcium of No.5 variety were higher those of the other 2 varieties,which was recognized as the most suitable variety for fresh fruit;(ii)the comparison of leaf areas index and fruit shape index of 3 varieties shows that the fruit shape index and individual fruit weight of the introduced varieties were higher than those of the control,the diameter of individual fruit of the introduced varieties higher than that of the control indicated that they had a higher fruit hardness,more dry matter accumulation and better fruit quality,and that the introduction region was more suitable for scale-up of P.humilis;(iii)there was a positive correlation with the number of fruiting branches and there was a significant negative correlation with individual fruit weight,while the leaf area had a significant negative correlation with the number of fruiting branches.The introduced varieties can develop normally in the 2 testing areas,notably,No.5 variety performed better than No.4 and No.6.Improving management is the prerequisite for maintaining proper number of fruiting branches and high yield. 展开更多
关键词 PRUNUS humilis Biological TRAITS NUTRITIONAL components Introduction and CULTIVATION
下载PDF
Pectin fractions extracted sequentially from Cerasus humilis:Their compositions,structures,functional properties and antioxidant activities
6
作者 Shikai Zhang Geoffrey INWaterhouse +2 位作者 Tingting Cui Dongxiao Sun-Waterhouse Peng Wu 《Food Science and Human Wellness》 SCIE CSCD 2023年第2期564-574,共11页
Three pectin fractions(water-soluble fraction(WSF),chelator-soluble fraction(CSF),and sodium carbonatesoluble fraction(NSF))were obtained from Chinese dwarf cherry(Cerasus humilis)fruits.All of them were branched low ... Three pectin fractions(water-soluble fraction(WSF),chelator-soluble fraction(CSF),and sodium carbonatesoluble fraction(NSF))were obtained from Chinese dwarf cherry(Cerasus humilis)fruits.All of them were branched low methoxylated pectins with an amorphous or partially nanocrystalline nature and eight neutral monosaccharides(arabinose and galactose were most abundant).WSF,CSF and NSF had a degree of methylation(DM)of 35.82%,14.85%and 7.13%,uronic acid(UA)content of 76.02%,83.71%and 69.01%,and total protein content of 2.4%,2.1%and 8.8%,respectively.Their molecular weights were 340.31,330.16 and 141.31 kg/mol,respectively(analyzed by gel permeation chromatography(GPC)).WSF,CSF and NSF exhibited good rheological,thermal,emulsifying,emulsion-stabilizing,water-adsorbing,oil-binding,cholesterol-binding and antioxidant properties.NSF had the highest emulsifying,emulsion stabilizing,water-/oil-/cholesterol-binding and antioxidant capacities,followed by CSF.NSF had the highest viscosity(406.77 m Pa·s),flowability,and resistance to heat-induced changes/damage,which may be related to its lowest polydispersity index,DM and UA content and highest protein content.The three pectin fractions with desirable characteristics can be used as food additives/ingredients and dietary supplements. 展开更多
关键词 Cerasus humilis fruits Pectin fractions Structural characteristics Processing-related functional properties
下载PDF
Three Phases of a Person's Ruin:After watching the Movie Rice
7
作者 严琦 冷小利 《海外英语》 2014年第19期191-192,共2页
Rice is regarded as a grey film because of its theme of human's evil nature. The dark, secret, and evil sides of human are vividly displayed, which arouse deep thoughts. How is the protagonist, Wulong, ruined? Wha... Rice is regarded as a grey film because of its theme of human's evil nature. The dark, secret, and evil sides of human are vividly displayed, which arouse deep thoughts. How is the protagonist, Wulong, ruined? What to blame for his downfall? In this essay, the writer would try to analyze the phases of his spiritual downfall and discuss the elements which influence the change of his character through a psychological analysis. 展开更多
关键词 the split of PERSONALITY humiliation REVENGE
下载PDF
欧李种仁中苦杏仁苷的提取及其抗氧化活性 被引量:8
8
作者 邬晓勇 孙雁霞 +2 位作者 何钢 赵琦 苟小军 《湖北农业科学》 北大核心 2013年第19期4764-4767,共4页
采用HPLC法测定欧李(Prunus humilis Bunge)种仁浓缩液中苦杏仁苷的含量,利用DPPH法测定苦杏仁苷的抗氧化性。结果表明,提取液中苦杏仁苷的浓度为7.94 mg/mL;苦杏仁苷清除DPPH的能力随着其浓度的降低而下降,当苦杏仁苷溶液浓度为7.50 mg... 采用HPLC法测定欧李(Prunus humilis Bunge)种仁浓缩液中苦杏仁苷的含量,利用DPPH法测定苦杏仁苷的抗氧化性。结果表明,提取液中苦杏仁苷的浓度为7.94 mg/mL;苦杏仁苷清除DPPH的能力随着其浓度的降低而下降,当苦杏仁苷溶液浓度为7.50 mg/mL时清除率最高,为90.9%。欧李种仁浓缩液经氯仿、乙酸乙酯和加热处理均能提高水相中苦杏仁苷的纯度。 展开更多
关键词 欧李(Prunus humilis Bunge) 种仁 苦杏仁苷 高效液相色谱 抗氧化性
下载PDF
鹿角珊瑚人工养殖的初步研究 被引量:9
9
作者 鲍鹰 周学家 +5 位作者 黄美霞 梁凯珊 黄雪芬 黄少秋 邱庆昌 郑小欧 《海洋科学》 CAS CSCD 北大核心 2012年第1期69-72,共4页
为了探讨珊瑚人工养殖的基本条件和方法,分别在陆基水泥池中和海底珊瑚苗床上对3种鹿角珊瑚:粗野鹿角珊瑚(Acropora humilis)、霜鹿角珊瑚(Acropora pruinosa)和松枝鹿角珊瑚(Acropora brueggemanni)的人工养殖进行了实验。结果表明,这... 为了探讨珊瑚人工养殖的基本条件和方法,分别在陆基水泥池中和海底珊瑚苗床上对3种鹿角珊瑚:粗野鹿角珊瑚(Acropora humilis)、霜鹿角珊瑚(Acropora pruinosa)和松枝鹿角珊瑚(Acropora brueggemanni)的人工养殖进行了实验。结果表明,这3种珊瑚可以在深圳市鹅公湾近岸海域的海底珊瑚苗床上常年生长;在水泥池中当水温低于14℃或高于28℃时珊瑚陆续死亡;这3种珊瑚的生长速度在适温范围内随着水温的升高而加快,最大月生长值是6.05 mm;光照对这3种珊瑚的生长有影响,直接的阳光照射会引起水泥池中珊瑚的死亡。 展开更多
关键词 鹿角珊瑚(Acropora humilis) 养殖
下载PDF
欧李浑浊果汁加工工艺的研究 被引量:2
10
作者 王芹 蔡金星 +2 位作者 刘秀凤 庞杰 张长峰 《山东农业科学》 2012年第6期110-115,共6页
以欧李为原料,采用单因素试验确定欧李果浆加水量、适合稳定剂,采用正交试验筛选酶解最佳条件和最佳配方。随着加水量的增加,欧李果浆粘稠度降低,欧李原有香味变淡;适当酶解条件下欧李原果汁的出汁率显著提高;糖浓度为10%、原果汁浓度65... 以欧李为原料,采用单因素试验确定欧李果浆加水量、适合稳定剂,采用正交试验筛选酶解最佳条件和最佳配方。随着加水量的增加,欧李果浆粘稠度降低,欧李原有香味变淡;适当酶解条件下欧李原果汁的出汁率显著提高;糖浓度为10%、原果汁浓度65%、柠檬酸浓度0.12%~0.16%的果汁口味、颜色较佳;黄原胶单独使用时的浑浊果汁稳定性良好;制得成品营养丰富。制备欧李果浆的加水量(质量比):欧李∶水=1∶2;最佳酶解条件:果胶酶浓度0.05%,酶解温度30℃,酶解时间1.5 h;最佳配方(质量分数):原汁浓度65%,糖浓度10%,柠檬酸浓度0.12%;稳定剂选择黄原胶,浓度0.1%。本研究为欧李浑浊果汁加工与存放中的稳定性提供技术保障,拓宽了欧李果实的加工利用途径。 展开更多
关键词 欧李(PmnW humilis) 浑浊果汁 酶解 稳定性
下载PDF
欧李嫁接技术 被引量:1
11
作者 张永信 安传志 李辉 《河北果树》 2016年第4期46-47,共2页
欧李(Gerasus humilis(Bge.)Sok)是蔷薇科樱属的一种落叶小灌木,因其果实含钙量居水果之首也称"钙果",是我国北方特有的第3代水果典型树种。欧李树体矮小,株高1.2-1.5 m,丛生。根系发达,根冠比为9∶1。4月开白花或粉红花,形似樱花... 欧李(Gerasus humilis(Bge.)Sok)是蔷薇科樱属的一种落叶小灌木,因其果实含钙量居水果之首也称"钙果",是我国北方特有的第3代水果典型树种。欧李树体矮小,株高1.2-1.5 m,丛生。根系发达,根冠比为9∶1。4月开白花或粉红花,形似樱花,极具观赏性,果似樱桃,味似李子,酸甜爽口,风味独特。单果质量5-15 g,果肉厚。果实有红、黄、紫等多种颜色。根系由主根、侧根组成,垂直分布达1-2 m,侧根伸向四周1-1.5 m,为深根性植物。 展开更多
关键词 欧李 嫁接技术 humilis 钙果 含钙量 樱属 单果质量 深根性 根冠比 垂直分布
下载PDF
浙江发现解氏狭臀蚤
12
作者 卢苗贵 赵芝雅 +4 位作者 叶小娜 叶仙萍 胡萍 吴军民 陈用琴 《浙江预防医学》 1991年第1期41-41,共1页
最近,在鉴定从浙江省义乌市采到的蚤类标本中发现狭臀蚤属(Genus Stenischia Jordan,1932)解氏狭臀蚤(S.Xiei Li.1987)一雌蚤,为本省首次纪录。现予报道。鉴别特征本蚤与奇异狭臀蚤(S.mirabilis Jordan,1932)、金氏狭臀蚤(S.chini Xie e... 最近,在鉴定从浙江省义乌市采到的蚤类标本中发现狭臀蚤属(Genus Stenischia Jordan,1932)解氏狭臀蚤(S.Xiei Li.1987)一雌蚤,为本省首次纪录。现予报道。鉴别特征本蚤与奇异狭臀蚤(S.mirabilis Jordan,1932)、金氏狭臀蚤(S.chini Xie et Lin,1989)和低地狭臀蚤(S.humilis Xie 展开更多
关键词 低地狭臀蚤 humilis GENUS 鉴别特征 蚤属 金氏 蚤类 JORDAN 颊栉 浙江省义乌市
下载PDF
不同海拔高度矮嵩草的光合响应差异 被引量:10
13
作者 韦玉 李熙萌 +2 位作者 桑卫国 周芸芸 冯金朝 《生态科学》 CSCD 北大核心 2014年第6期1160-1164,共5页
高海拔地区具有强光辐射、低CO2分压和低温等环境特征。长期生长在高海拔地区的植物各自形成了其独特的适应特性。为了揭示矮嵩草(Kobresia humilis)对不同海拔的光合生理适应机制,使用Li-6400便携式光合作用测量系统观测了大阪山阴坡... 高海拔地区具有强光辐射、低CO2分压和低温等环境特征。长期生长在高海拔地区的植物各自形成了其独特的适应特性。为了揭示矮嵩草(Kobresia humilis)对不同海拔的光合生理适应机制,使用Li-6400便携式光合作用测量系统观测了大阪山阴坡生长于3个不同海拔高度(3000 m、3400 m、3800 m)矮嵩草光响应和CO2响应曲线。结果显示,在测量温度下,矮嵩草的最大净光合速率和光合能力随海拔梯度升高而逐渐提高;高海拔地区生长的矮嵩草具有较高的羧化效率,而低海拔地区生长的矮嵩草具有较高的表观量子效率。高海拔地区生长的矮嵩草具较高的光饱和点,较低的CO2补偿点和饱和点,而不同海拔间的光呼吸速率无显著差异。研究结果表明,矮嵩草表现出对高海拔地区环境积极的适应方式。 展开更多
关键词 矮嵩草(Kobresia humilis) 海拔梯度 光响应曲线 CO2响应曲线
下载PDF
从嵩草属植物看生物多样性
14
作者 杨勇刚 杨福囤 《青海环境》 1994年第4期170-173,共4页
保护生物多样性日益引起人们的重视,也是当前国际上重大的环境问题之一。由于生物多样性是人类赖以生存的条件和经济发展的物质基础,故1992年6月在巴西召开了联合国环境与发展大会,大多数国家元首或政府首脑在《生物多样性公约》上签字... 保护生物多样性日益引起人们的重视,也是当前国际上重大的环境问题之一。由于生物多样性是人类赖以生存的条件和经济发展的物质基础,故1992年6月在巴西召开了联合国环境与发展大会,大多数国家元首或政府首脑在《生物多样性公约》上签字。提高环境保护意识已刻不容缓。1994年6月12日我国又召开专门会议,并正式开始实施《中国生物多样性保护行动计划》。 展开更多
关键词 嵩草 生物多样性 环境问题 高山唐松草 黑褐苔草 政府首脑 杂类草 矮火绒草 异针茅 humilis
下载PDF
欧李优系组培快繁技术研究 被引量:3
15
作者 梁伟玲 陈翠果 +3 位作者 孟繁祎 赵敏 孙华冉 刘艳芬 《湖北农业科学》 2019年第6期136-139,共4页
为建立欧李(Cerasus humilis)优系离体快繁技术体系,对其离体培养过程中初代培养、增殖继代培养、生根培养3个阶段的培养条件进行了研究。结果表明,以其半木质化茎段为外植体,最适初代培养基为MS+0.5 mg/L 6-BA+0.1 mg/L IBA,最适增殖... 为建立欧李(Cerasus humilis)优系离体快繁技术体系,对其离体培养过程中初代培养、增殖继代培养、生根培养3个阶段的培养条件进行了研究。结果表明,以其半木质化茎段为外植体,最适初代培养基为MS+0.5 mg/L 6-BA+0.1 mg/L IBA,最适增殖培养基为MS+0.5 mg/L 6-BA+0.5 mg/L KT+0.05 mg/L IBA+300 mg/L水解乳蛋白,最适生根培养基为1/2MS+0.2 mg/L IBA+0.5 mg/L IAA。 展开更多
关键词 欧李(Cerasus humilis) 组织培养 快速繁殖
下载PDF
Transcriptomic comparison to identify rapidly evolving genes in Braya humilis
16
作者 YuMing Wei XiaoFei Ma PengShan Zhao 《Research in Cold and Arid Regions》 CSCD 2018年第5期428-435,共8页
The Brassicaceae species Braya humilis shows broad adaptation to different climatic zones and latitudes. However, the molecular adaptation mechanism of B. humilis is poorly understood. In China, B. humilis is mainly d... The Brassicaceae species Braya humilis shows broad adaptation to different climatic zones and latitudes. However, the molecular adaptation mechanism of B. humilis is poorly understood. In China, B. humilis is mainly distributed on the QinghaiTibetan Plateau(QTP) and in the adjacent arid region. Previous transcriptome analysis of B. humilis has revealed that 39 salt and osmotic stress response genes are subjected to purifying selection during its speciation. To further explore the adaptation mechanism of B. humilis to an arid environment, OrthoMCL program was employed in this study and 6,268 pairs of orthologous gene pairs with high confidence were obtained between B. humilis and Arabidopsis thaliana. A comparative evolutionary analysis based on nonsynonymous to synonymous substitution ratio(Ka/Ks) was then conducted. There were 64 pairs exhibiting a Ka/Ks ratio more than 0.5 and among which, three instrumental candidate genes, T20487,T22576, and T23757, were identified with strong selection signatures(Ka/Ks >1). The corresponding A. thaliana orthologs are double-stranded RNA-binding domain protein, MADS-box family protein, and NADH-dehydrogenase subunit6, which is encoded by mitochondria genome. This report not only demonstrates the adaptation contribution of fast evolving nuclear genes, but also highlights the potential adaptive value of mitochondria gene to the speciation and adaptation of B. humilis toward the extreme environment in an arid region. 展开更多
关键词 Braya humilis positive selection Ka/Ks mitochondria gene variation NADH-dehydrogenase subunit 6
下载PDF
Characteristics of Kobresia humilis Community Structure at Different Degraded Levels in Northern Qinghai
17
作者 SHI Hui-lan 《Animal Husbandry and Feed Science》 CAS 2011年第4期39-42,共4页
[ Objective] To reveal structure and function of alpine meadow ecosystem and thus to provide a scientific basis for development, utilization and scientific management of alpine meadow pasture as well as sustainable de... [ Objective] To reveal structure and function of alpine meadow ecosystem and thus to provide a scientific basis for development, utilization and scientific management of alpine meadow pasture as well as sustainable development of grassland agriculture. [ Method] Charactedstics of Kobresia humilis communities with primary vegetation (community I) and degraded vegetation (community II) were analyzed. [Result] Species richness, biodiversity index and biomass of the community I were respectively 42, 3. 531 and 3 553.1 g/m^2, which were respectively higher than those of the community II (37, 2.270 and 3 391.1 g/m^2). Correlation analysis shows that community biomass was correlated positively with the dchness index ( P 〈 0.01 ), and biodiversity index was correlated positively with the aboveground biomass and dchness index ( P 〈 0.01 ). [ Conclusion] The Kobresia humilis community I has reasonable structure and large coverage of ground vegetation, which play an important role in maintenance of biodiversity and ecosystem function. 展开更多
关键词 Kobresia humilis meadow Community structure Species diversity
下载PDF
Contribution of Chamaerops humilis L. (Arecaceae) in Conservation in Situ of Soil Resources in the Tlemcen Region (Western Algeria)
18
作者 Naima Brahimi Hasnaoui Okkacha Cherif Ismahan 《Open Journal of Ecology》 2016年第9期562-567,共6页
In recent years, the anthropic action on scrubs in the region of Tlemcen (a province in western Algeria) has represented a threat to the groups of Chamaerops humilis L. (Arecaceae). The main purpose of the present inv... In recent years, the anthropic action on scrubs in the region of Tlemcen (a province in western Algeria) has represented a threat to the groups of Chamaerops humilis L. (Arecaceae). The main purpose of the present investigation is to highlight the relationship between Chamaerops humilis and its biotope. Taking advantage of the refurbishment works in the study area, clumps of Chamaerops humilis, freshly dug by machinery, were collected and used to conduct some measurements. A total of 34 clusters were considered. The main parameters to be studied are: 1. The height of the tangle of roots and the number of roots in each cluster;2. The weight of soil trapped between roots;3. Physico-chemical properties of soil in which taxa grow. The results obtained showed that the average weight of soil retained by the clumps was important (70.3 kg);the average height of the tangle was 68.4 cm and the average number of roots was 382. The tangle of roots, of various stocks of Chamaerops humilis, creates a root network, very tangled in its upper part, thereby trapping a significant amount of soil. The statistical analyses of pairs of parameters, considered in the present work (height of the tangle, mass of soil trapped by roots and number of roots), were found to be highly correlated. These analyses allowed identifying the relationships existing between the studied parameters. They also helped to deduce the role of the taxon in the in situ conservation of edaphic potential. 展开更多
关键词 Chamaerops humilis Morphometric Measurements Soil CONSERVATION Tlemcen
下载PDF
Comprehensive transcriptomic analysis of developing seed in Cerasus humilis for lipid related gene discovery
19
作者 Xiaopeng Mu Junjie Du +1 位作者 Pengfei Wang Jiancheng Zhang 《Oil Crop Science》 2019年第3期127-138,共12页
Cerasus humilis is a woody shrub with increasing economic importance. Due to its high oil content in seed with desired fatty acid(FA) composition, C. humilis has been considered as a potential woody oilseed crop in Ch... Cerasus humilis is a woody shrub with increasing economic importance. Due to its high oil content in seed with desired fatty acid(FA) composition, C. humilis has been considered as a potential woody oilseed crop in China. However, FAs accumulation and related molecular mechanisms of FA biosynthesis in C. humilis seed have not been elucidated well. In this study, oil contents and FA compositions of developing C. humilis seed were analyzed. The total oil content in mature seed reached 48.7% while unsaturated FA concentration reached a high level of 96.2%. Three c DNA libraries of C. humilis developing seed were constructed at the beginning and 2 fast oil-accumulation stages, followed by Illumina sequencing with the platform of HiSeq^TM 2000. Differentially expressed unigenes(DEGs) were identified during the respective seed development stages to investigate transcription dynamics. Among DEGs, 82 unigenes were identified as being closely involved in de novo FA and triacylglycerol(TAG) biosynthesis. Surprisingly, among DEGs involved in TAG biosynthesis, expression of unigenes encoding GPATs(glycerol-3-phosphate acyltransferase) were relatively low and unigenes encoding LPAATs(lysophosphatidic acid acyltransferase) were highly expressed, suggesting that LPAAT contributed more in storage lipids metabolism in C. humilis seed. Genes encoding DGAT2(diacylgycerol acyltransferase2) were the most highly expressed while expression of DGAT1 was very low, suggesting DGAT2 was the dominant DGAT which catalyzed TAG biosynthesis. To verify these results,10 unigenes were selected and their expression patterns were analyzed by quantitative RTPCR. These data provided comprehensive information for understanding the molecular mechanism of FA and TAG biosynthesis in C. humilis seed. 展开更多
关键词 Cerasus humilis FATTY acid SEED development RNA-SEQ TRIACYLGLYCEROL BIOSYNTHESIS
下载PDF
欧李ChUFGT基因的克隆、生物信息学分析及亚细胞定位 被引量:1
20
作者 王迪 成冠超 +3 位作者 祝利伟 刘宇涵 笈小龙 宋兴舜 《分子植物育种》 CAS 北大核心 2023年第3期782-788,共7页
为从分子水平上揭示欧李中3-O-类黄酮葡萄糖基转移酶(UFGT)基因的空间表达情况,以欧李叶片为试材,根据欧李的转录组序列设计特异性引物,通过PCR获得ChUFGT基因序列,并进行生物信息学分析及亚细胞定位分析。结果表明,ChUFGT基因DNA开放... 为从分子水平上揭示欧李中3-O-类黄酮葡萄糖基转移酶(UFGT)基因的空间表达情况,以欧李叶片为试材,根据欧李的转录组序列设计特异性引物,通过PCR获得ChUFGT基因序列,并进行生物信息学分析及亚细胞定位分析。结果表明,ChUFGT基因DNA开放阅读框为1425 bp(不含终止子),相对分子质量为51.63 kD,编码475个氨基酸,其对应的蛋白质是带正电荷的稳定疏水性蛋白。与近缘物种的蛋白质序列比对分析显示,欧李、李和樱桃李的UFGT氨基酸序列相似度达到97.22%和96.62%,整个系统发育树存在两个大分支,其中ChUFGT与同科植物的亲缘关系最近,其次是与白梨、西洋梨、荔枝等其他科植物相对较近,与葡萄、刺葡萄、石榴等植物的亲缘关系最远。ChUFGT的亚细胞定位结果分析显示,在洋葱内表皮细胞的细胞核和细胞质中都表达该基因。本研究结果为进一步阐明ChUFGT调控欧李细胞花色素苷的合成机制提供了理论基础。 展开更多
关键词 欧李(Cerasus humilis) UFGT基因 基因克隆 生物信息学分析 亚细胞定位
原文传递
上一页 1 2 下一页 到第
使用帮助 返回顶部