Biomaterial research has been going on for several years,and many companies are heavily investing in new product development.However,it is a contentious field of science.Biomaterial science is a field that combines ma...Biomaterial research has been going on for several years,and many companies are heavily investing in new product development.However,it is a contentious field of science.Biomaterial science is a field that combines materials science and medicine.The replacement or restoration of damaged tissues or organs enhances the patient’s quality of life.The deciding aspect is whether or not the body will accept a biomaterial.A biomaterial used for an implant must possess certain qualities to survive a long time.When a biomaterial is used for an implant,it must have specific properties to be long-lasting.A variety of materials are used in biomedical applications.They are widely used today and can be used individually or in combination.This review will aid researchers in the selection and assessment of biomaterials.Before using a biomaterial,its mechanical and physical properties should be considered.Recent biomaterials have a structure that closely resembles that of tissue.Antiinfective biomaterials and surfaces are being developed using advanced antifouling,bactericidal,and antibiofilm technologies.This review tries to cover critical features of biomaterials needed for tissue engineering,such as bioactivity,self-assembly,structural hierarchy,applications,heart valves,skin repair,bio-design,essential ideas in biomaterials,bioactive biomaterials,bioresorbable biomaterials,biomaterials in medical practice,biomedical function for design,biomaterial properties such as biocompatibility,heat response,non-toxicity,mechanical properties,physical properties,wear,and corrosion,as well as biomaterial properties such surfaces that are antibacterial,nanostructured materials,and biofilm disrupting compounds,are all being investigated.It is technically possible to stop the spread of implant infection.展开更多
Glioblastoma(GBM,WHO grade IV glioma)is the most common and lethal malignant brain tumor in aduts with a dismal prognosis.The extracellular matrix(ECM)supports GBM progression by promoting tumor cell proliferation,mig...Glioblastoma(GBM,WHO grade IV glioma)is the most common and lethal malignant brain tumor in aduts with a dismal prognosis.The extracellular matrix(ECM)supports GBM progression by promoting tumor cell proliferation,migration,and immune escape.Uridine diphosphate(UDP)-glucose 6-dehydrogenase(UGDH)is the rate-limiting enzyme that catalyzes the biosynthesis of glycosaminoglycans that are the principal component of the CNS ECM.We investigated how targeting UGDH in GBM infuence$the GBM immune microenvironment,including tumor-associated microglia/macrophages(TAMs)and T cells.TAMs are the main im-mune effector cells in GBM and can directly target tumor cells if properly activated.In co-cultures of GBM cells and human primary macrophages,UGDH knockdown in GBM cells pro-moted macrophage phagocytosis and M-like polarization.In orthotropic human GBM xeno-grafts and syngeneic mouse glioma models,targeting UGDH decreased ECM deposition,increased TAM phagocytosis marker expression,reduced M2-like TAMS and inhibited tumor growth.UGDH knockdown in GBM cells also promoted cytotoxic T cell ifltration and activa-tion in orthotopic syngeneic mouse glioma models.The potent and in-human-use small mole-cule GAG synthesis inhibitor 4-methylumbelliferone(4-MU)was found to inhibit GBM cell proliferation and migration in vitro,mimic the macrophage and T-cell responses to UGDH knockdown in vitro and in vivo and inhibit growth of orthotopic murine GBM.Our study shows that UGDH supports GBM growth through multiple mechanisms and supports the development of ECM-based therapeutic strategies to simultaneously target tumor cells and their microenvi-ronment.展开更多
文摘Biomaterial research has been going on for several years,and many companies are heavily investing in new product development.However,it is a contentious field of science.Biomaterial science is a field that combines materials science and medicine.The replacement or restoration of damaged tissues or organs enhances the patient’s quality of life.The deciding aspect is whether or not the body will accept a biomaterial.A biomaterial used for an implant must possess certain qualities to survive a long time.When a biomaterial is used for an implant,it must have specific properties to be long-lasting.A variety of materials are used in biomedical applications.They are widely used today and can be used individually or in combination.This review will aid researchers in the selection and assessment of biomaterials.Before using a biomaterial,its mechanical and physical properties should be considered.Recent biomaterials have a structure that closely resembles that of tissue.Antiinfective biomaterials and surfaces are being developed using advanced antifouling,bactericidal,and antibiofilm technologies.This review tries to cover critical features of biomaterials needed for tissue engineering,such as bioactivity,self-assembly,structural hierarchy,applications,heart valves,skin repair,bio-design,essential ideas in biomaterials,bioactive biomaterials,bioresorbable biomaterials,biomaterials in medical practice,biomedical function for design,biomaterial properties such as biocompatibility,heat response,non-toxicity,mechanical properties,physical properties,wear,and corrosion,as well as biomaterial properties such surfaces that are antibacterial,nanostructured materials,and biofilm disrupting compounds,are all being investigated.It is technically possible to stop the spread of implant infection.
基金This work was supported by grants from NIH R01NS091165(S.X.),R01 NS099460(M Ying),R01 NS096754(J Laterra),and R01 NS076759(J Laterra)。
文摘Glioblastoma(GBM,WHO grade IV glioma)is the most common and lethal malignant brain tumor in aduts with a dismal prognosis.The extracellular matrix(ECM)supports GBM progression by promoting tumor cell proliferation,migration,and immune escape.Uridine diphosphate(UDP)-glucose 6-dehydrogenase(UGDH)is the rate-limiting enzyme that catalyzes the biosynthesis of glycosaminoglycans that are the principal component of the CNS ECM.We investigated how targeting UGDH in GBM infuence$the GBM immune microenvironment,including tumor-associated microglia/macrophages(TAMs)and T cells.TAMs are the main im-mune effector cells in GBM and can directly target tumor cells if properly activated.In co-cultures of GBM cells and human primary macrophages,UGDH knockdown in GBM cells pro-moted macrophage phagocytosis and M-like polarization.In orthotropic human GBM xeno-grafts and syngeneic mouse glioma models,targeting UGDH decreased ECM deposition,increased TAM phagocytosis marker expression,reduced M2-like TAMS and inhibited tumor growth.UGDH knockdown in GBM cells also promoted cytotoxic T cell ifltration and activa-tion in orthotopic syngeneic mouse glioma models.The potent and in-human-use small mole-cule GAG synthesis inhibitor 4-methylumbelliferone(4-MU)was found to inhibit GBM cell proliferation and migration in vitro,mimic the macrophage and T-cell responses to UGDH knockdown in vitro and in vivo and inhibit growth of orthotopic murine GBM.Our study shows that UGDH supports GBM growth through multiple mechanisms and supports the development of ECM-based therapeutic strategies to simultaneously target tumor cells and their microenvi-ronment.
