Effect of Huoxuequyu Recipe on Erythrocyte Membrane Ca^（2＋）－ATPase Activity in Pregnant Rats with Asymmetrical Intrauterine G

The effect of Huoxuequyu recipe on erythrocyte membrane Ca2+-ATPase activity in pregnant rats with passive smoking induced asymmetrical intrauterine growth retardation(IUGR)was observed.The rats were divided into three groups:control group,model group and treated group.The fetal mean birth weight and erythrocyte membrane Ca2+-ATPase activity were found to be decreased in the model group as compared with the control and treated groups.There was a significant difference between the model group and the control group(P<0.01),while no significant difference existed between the treated group and the control group(P>0.05).Furthermore,the findings indicated that the erythrocyte membrane Ca2+-ATPase activity seem to be positively correlated with the birth weight(P<0.05).On the basis of our observation,it is believed that the Huoxuequyu decoction could increase erythrocyte membrane Ca2+-ATPase activity and promote blood circulation, thereby exerting a beneficial effect on fetal development.

Effect of Huoxuequyu Recipe on Erythrocyte Ultrastructure and Membrane ATPase Activity in Rats with Passive Smoking

The effect of 'Huoxuequyu' recipe on erythrocyte membrane Ca2+ATPase activity, Na+-K+-ATPase activity and erythrocyte ultrastructure in rats exposed to passive smoking were observed. The erythrocyte membrane Ca2+-ATPase activity and Na+-K+-ATPase activity were found to be decreased in the model group as compared with the control group (P<0.01, P<0.01), while no significant difference existed between the treated group and the control group (P>0.05). Our results also showed that there were more deformed red blood cells in model group than in control group under TCM and SEM (P<0.0001). No significant difference was found between control group and treated group (P>0.05). Furthermore,these findings indicated that 'Huoxuequyu' recipe , a prepared natural herbal medicine, may be involved in modulation of the activity of erythrocyte membrane enzymes,thereby protecting erythrocyte membrane, so as to improve the erythrocyte function.

The Effects of "Huoxuequyu" Decoction on Lipid Peroxidation in IUGR Rats

The rats exposed to passive smoking were used as the animal model to further study the pathogenesis of IUGR and the effects of ' Huoxuequyu' decoction. The results showed that the mean birth-weight of fetus and SOD activity of placenta and plasma in model rats were significantly reduced, and LPO levels of placenta and plasma were significantly increased. We found that the mean birthweight and SOD activity in treated group were higher than that in model group,and LPO levels of placenta and plasma were lower in treated group than that in model group and there was no difference in SOD activity and LPO levels of placenta and plasma as well as the mean birthweight between treated group and control group, indicating that 'Huoxuequyu' decoction can protect placenta and plasma against lipid peroxidation, and improve the blood circulation of uteroplacenta, thus contributing to the increase of the mean birth-weight.

活血化瘀方对被动吸烟孕鼠红细胞膜Ca^(2+)-ATP酶活性的影响

以被动吸烟致胎儿宫内发育延缓（IUGR）大鼠为模型，观察了活血化瘀方对孕鼠红细胞膜Ca^(2+)－ATP酶活性的影响。结果表明：模型组胎仔出生平均体重、Ca^（2+）-ATP酶活力均低于对照组（P＜0.01），用药组与对照组之间差异无显著意义（P＞0.05），且胎仔平均出生体重与Ca^(2+)-ATP酶活性呈正相关关系（r=0.3446,P＜O.05）。从而提示本方能提高Ca^(2+)-ATP酶活性，保护红细胞正常形态与功能,从而改善胎盘血液循环，促进胎儿发育。

Effect of "Jia Wei Fo Shou San" on Erythrocyte Membrane Calcium,Zinc Concentration in Pregnant Rats with Asymmetrical Intrauterine Growth Retardation Induced by Passive Smoking

Using experimental model of pregnant rats with asymmetrical intrauterine fetal growth retardation induced by passive smoking, the effects of natural herb 'Jia Wei Fo Shou San' on erythrocyte membrane calcium, zinc concentration were observed by atomic absorption spectrophotometry. The results showed that the mean fetal birth weight, zinc concentration of erythrocyte membrane were found to be decreased in the model group as compared with the control group (P＜0. 01, P＜0. 01). On the other hand, the element of calcium onto erythrocyte membrane were higher in model group than that in control group(P＜0. 05). These changes were significantly mild in the treated group and were similar to those of the control group (P＜0.05). Furthermore,our findings indicated that the zinc concentration of erythrocyte membrane seems to be positively correlated with the birth weight(P＜0. 01). Calcium composition of red cell membrane showed a significant negative relation to the birth weight (P＜0.05). Our results provided an experimental evidence that normal concentrations of calcium and zinc onto erythrocyte play an important part in fetal growth. One of the mechanisms of 'Jia Wei Fo Shou San' in improving fetal growth may have something to do with modulation of erythrocyte calcium,zinc element,thereby protecting bio-functions of erythrocyte and promoting blood circulation.

活血去瘀颗粒的水提取工艺研究

目的研究活血去瘀颗粒的最佳水提取工艺。方法用正交试验设计优化最佳提取条件,以浸膏得率和丹参素含量为指标,选用L9(34)进行煎煮条件的选择。结果最佳水提取工艺为加入8倍量水提取3次,每次煎煮1.5 h。结论该提取工艺所得浸膏得率和丹参素含量高。并经验证,合理可行。

新伤汤及活血祛瘀散治疗膝关节急性创伤性滑膜炎的疗效观察

目的：观察新伤汤及活血祛瘀散治疗膝关节急性创伤性滑膜炎的疗效。方法2014年8月～2015年12月期间入院及门诊治疗的膝关节急性创伤性滑膜炎患者共60例。将该60例患者作为研究对象随机分为治疗组和对照组，每组30例。两组患者均给予基础治疗。除此之外，治疗组给予本院中医骨伤科验方新伤汤内服及活血祛瘀散外用；对照组给予中医经典方桃红四物汤内服及双柏散外敷。两组患者治疗疗程均为1个月。评价两组患者的临床治疗疗效。比较两组患者治疗前、治疗过程中第2周、治疗结束后VAS评分、Bristol评分及用药安全性。与治疗结束后评定两组患者的Liket评分、LLiket评分。观察并记录两组患者治疗过程中的不良反应情况。结果治疗前及治疗过程中第2周，两组患者的VAS评分、Bristol膝关节评分差异不明显（P＞0.05）。治疗结束后，两组患者的VAS评分、Bristol膝关节评分差异具有统计学意义（P＜0.05）。两组患者治疗前、治疗过程中第2周、治疗结束后三个时间点VAS评分、Bristol膝关节评分差异具有统计学意义（P＜0.05）。对照组患者治疗前后Liket评分、治疗组患者治疗前后Liket评分和LLiket评分差异具有统计学意义（P＜0.05）。治疗后，两组患者Liket评分、LLiket评分差异具有统计学意义（ P＜0.05）。两组患者治疗前后血、尿、便常规及肝、肾功能，心电图检查均未见异常。两组患者治疗前后均出现不同程度的皮疹、皮肤红潮等过敏反应，对照组不良反应发生率为16.67%（5/30），治疗组不良反应发生率为10.00%（3/30），两组患者不良反应发生情况差异无统计学意义（P＞0.05）。结论中医药治疗相比经典中药治疗疗效显著，患者治疗后疼痛程度减轻，安全可靠，值得在临床推广使用。