[Objective] The aim was to study the effects of drought stress on contents of endogenous hormones in leaves of different clones of Liriodendron chinense×L. tulipifera. Drought-resistant Liriodendron chinense×...[Objective] The aim was to study the effects of drought stress on contents of endogenous hormones in leaves of different clones of Liriodendron chinense×L. tulipifera. Drought-resistant Liriodendron chinense×L. tulipifera clones NE25,NE78 and drought-sensitive Liriodendron chinense×L. tulipifera clones NE04,NE23 were selected as experimental materials to study the content changes of four kinds of endogenous hormones such as abscisic acid （ABA）,indoleacetic acid （IAA）,ribosylzeatin （ZR） and jasmonic acid （JA） in leaves of different clones under drought stress. [Result] Under drought stress,ABA was accumulated I leaves of different clones,however,the content changes in drought-resistant clones were not obvious; IAA content showed a decreasing trend,and the content changes n drought-resistant clones during later period of drought stress was decreased; JA content showed an increasing trend in leaves of drought-sensitive clones,indicating that drought-sensitive clones had to maintain higher contents of JA to resist drought stress. [Conclusion] The result of this study had provided scientific basis for the screening,planting and application of drougt-resistant clones of Liriodendron chinense×L. tulipifera.展开更多
With the leaves,bark and roots of Liriodendron chinense Sarg.× L.tulipifera L.as experiment materials,the ultrasonic-assisted extraction conditions of total flavonoids were optimized by response surface Box-Behnk...With the leaves,bark and roots of Liriodendron chinense Sarg.× L.tulipifera L.as experiment materials,the ultrasonic-assisted extraction conditions of total flavonoids were optimized by response surface Box-Behnken test design.Ultrasonic time and liquid-to-solid ratio had significant effects on the extraction amount of flavonoids from the leaves of L.chinense Sarg.× L.tulipifera L.;ultrasonic temperature and liquid-to-solid ratio had an interaction on the extraction amount of flavonoids;and the optimum ultrasonic time,temperature and liquid-to-solid ratio for the extraction of flavonoids from leaves of L.chinense Sarg.× L.tulipifera L.were 19.82 min,28.60 ℃ and 9.48 ml/g,respectively.Ultrasonic time,temperature and liquid-to-solid ratio had significant effects on the extraction amount of flavonoids from L.chinense Sarg.× L.tulipifera L.;there was a significant interaction between any two of the factors,which had a significant effect on the extraction of flavonoids;and the optimum extraction parameters for flavonoids in the bark were the ultrasonic time of 33.66 min,the ultrasonic temperature at 32.85 ℃ and the liquid-to-solid ratio of 11.39 ml/g.Ultrasonic time and liquid-to-solid ratio had significant effects on the extraction of flavonoids from roots;there was a significant interaction between ultrasonic time and liquid-to-solid ratio and between ultrasonic temperature and liquid-to-solid ratio;and the optimum extraction parameters for flavonoids from the roots of L.chinense Sarg.× L.tulipifera L.were the ultrasonic time of 32.38 min,the ultrasonic temperature at 25.0 ℃ and the liquid-to-solid ratio of 8.00 ml/g.The results of the three models were stable.The models are feasible and have good application value.展开更多
[Objectives]This study was conducted to investigate the volatile components of Polygonum chinense L.[Methods]The volatile components of the methanol extract,ethyl acetate extract,chloroform extract and petroleum ether...[Objectives]This study was conducted to investigate the volatile components of Polygonum chinense L.[Methods]The volatile components of the methanol extract,ethyl acetate extract,chloroform extract and petroleum ether extract of P.chinense were analyzed and determined by gas chromatography.[Results]The volatile components of the methanol extract from P.chinense were the most,and phenolic acids and ketones accounted for a relatively high proportion,among which pyrogallic acid had the highest content.The ethyl acetate extract contained the second most volatile components,mostly acids and esters,of whichβ-sitosterol had the highest content;the chloroform and petroleum ether extracts had relatively few types of volatile components,most of which were alkanes,and the content ofγ-sitosterol shared by the two was the highest.The common substances of the four extracts were palmitic acid and phytol.[Conclusions]This study provides a theoretical basis for the quality evaluation of P.chinense and a scientific basis for its resource development.展开更多
In order to solve the problem of low emergence rate of Bupleurum chinense seeds, and screen out the best germination conditions, the seeds of Bupleurum chinense L. were treated with different soaking time, germinatio...In order to solve the problem of low emergence rate of Bupleurum chinense seeds, and screen out the best germination conditions, the seeds of Bupleurum chinense L. were treated with different soaking time, germination temperature and exogenous regulating substances to improve the germination rate. The results showed that the germination rate of fresh water at 12 h was significantly higher than that at 8 h and 36 h; The germination rate of seeds treated with constant temperature 15℃ and variable temperature 25 ℃/15 ℃ （day/night） was significantly higher than that of other temperature treatments. Seed soaking time and culture temperature also influenced seed germination start day and peak day. Different concentrations of regulatory substances could promote seeds germination. The germination rate of seeds induced by 0.50 mg/L 6-BA was the highest, the start day and peak day were 4 days and 5 days earlier than control check （CK） respectively.展开更多
基金Supported by National Natural Science Foundation (30070633 )National Science and Technology Supporting Project during the Eleventh Five-year Plan(2007BAD67B02)~~
文摘[Objective] The aim was to study the effects of drought stress on contents of endogenous hormones in leaves of different clones of Liriodendron chinense×L. tulipifera. Drought-resistant Liriodendron chinense×L. tulipifera clones NE25,NE78 and drought-sensitive Liriodendron chinense×L. tulipifera clones NE04,NE23 were selected as experimental materials to study the content changes of four kinds of endogenous hormones such as abscisic acid （ABA）,indoleacetic acid （IAA）,ribosylzeatin （ZR） and jasmonic acid （JA） in leaves of different clones under drought stress. [Result] Under drought stress,ABA was accumulated I leaves of different clones,however,the content changes in drought-resistant clones were not obvious; IAA content showed a decreasing trend,and the content changes n drought-resistant clones during later period of drought stress was decreased; JA content showed an increasing trend in leaves of drought-sensitive clones,indicating that drought-sensitive clones had to maintain higher contents of JA to resist drought stress. [Conclusion] The result of this study had provided scientific basis for the screening,planting and application of drougt-resistant clones of Liriodendron chinense×L. tulipifera.
基金Supported by the Policy Guidance Plan of Jiangsu Province(Production and Research Cooperation)(BY2016061-27)College Students’Practice Innovation Training Program of Jiangsu Province(201811049045X)
文摘With the leaves,bark and roots of Liriodendron chinense Sarg.× L.tulipifera L.as experiment materials,the ultrasonic-assisted extraction conditions of total flavonoids were optimized by response surface Box-Behnken test design.Ultrasonic time and liquid-to-solid ratio had significant effects on the extraction amount of flavonoids from the leaves of L.chinense Sarg.× L.tulipifera L.;ultrasonic temperature and liquid-to-solid ratio had an interaction on the extraction amount of flavonoids;and the optimum ultrasonic time,temperature and liquid-to-solid ratio for the extraction of flavonoids from leaves of L.chinense Sarg.× L.tulipifera L.were 19.82 min,28.60 ℃ and 9.48 ml/g,respectively.Ultrasonic time,temperature and liquid-to-solid ratio had significant effects on the extraction amount of flavonoids from L.chinense Sarg.× L.tulipifera L.;there was a significant interaction between any two of the factors,which had a significant effect on the extraction of flavonoids;and the optimum extraction parameters for flavonoids in the bark were the ultrasonic time of 33.66 min,the ultrasonic temperature at 32.85 ℃ and the liquid-to-solid ratio of 11.39 ml/g.Ultrasonic time and liquid-to-solid ratio had significant effects on the extraction of flavonoids from roots;there was a significant interaction between ultrasonic time and liquid-to-solid ratio and between ultrasonic temperature and liquid-to-solid ratio;and the optimum extraction parameters for flavonoids from the roots of L.chinense Sarg.× L.tulipifera L.were the ultrasonic time of 32.38 min,the ultrasonic temperature at 25.0 ℃ and the liquid-to-solid ratio of 8.00 ml/g.The results of the three models were stable.The models are feasible and have good application value.
基金Supported by Development of Huotanmu Capsules,a New Drug for Hepatitis B Treatment(20183046-1)Collaborative Innovation Center for Zhuang and Yao Medicines(GJKY[2013]20)+2 种基金Guangxi Key Laboratory of Zhuang and Yao Medicines(GKJZ[2014]32)Guangxi Key Discipline:Zhuang Medicine(GJKY[2013]16)First-class Discipline in Guangxi of Traditional Chinese Pharmacology(GJKY[2018]12)。
文摘[Objectives]This study was conducted to investigate the volatile components of Polygonum chinense L.[Methods]The volatile components of the methanol extract,ethyl acetate extract,chloroform extract and petroleum ether extract of P.chinense were analyzed and determined by gas chromatography.[Results]The volatile components of the methanol extract from P.chinense were the most,and phenolic acids and ketones accounted for a relatively high proportion,among which pyrogallic acid had the highest content.The ethyl acetate extract contained the second most volatile components,mostly acids and esters,of whichβ-sitosterol had the highest content;the chloroform and petroleum ether extracts had relatively few types of volatile components,most of which were alkanes,and the content ofγ-sitosterol shared by the two was the highest.The common substances of the four extracts were palmitic acid and phytol.[Conclusions]This study provides a theoretical basis for the quality evaluation of P.chinense and a scientific basis for its resource development.
文摘In order to solve the problem of low emergence rate of Bupleurum chinense seeds, and screen out the best germination conditions, the seeds of Bupleurum chinense L. were treated with different soaking time, germination temperature and exogenous regulating substances to improve the germination rate. The results showed that the germination rate of fresh water at 12 h was significantly higher than that at 8 h and 36 h; The germination rate of seeds treated with constant temperature 15℃ and variable temperature 25 ℃/15 ℃ （day/night） was significantly higher than that of other temperature treatments. Seed soaking time and culture temperature also influenced seed germination start day and peak day. Different concentrations of regulatory substances could promote seeds germination. The germination rate of seeds induced by 0.50 mg/L 6-BA was the highest, the start day and peak day were 4 days and 5 days earlier than control check （CK） respectively.
