Effect of Daidzein on Ileum Microflora Biodiversity in Hy-Line Variety Brown Layers

Daidzein is always added into poultry feed to make the production performance and immunity of poultry better. In this study, a total of 600 40-week-old Hy-Line variety brown layers were randomized into five groups and fed with a corn-soybean-mixed basal diet supplement with 0, 10, 50, 100, and 500 mg · kg^-1 daidzein, respectively. Then, two PCR-based typing methods（RAPD-PCR and ERIC-PCR） were combined to analyze the ileum content and explore the changes of ileum microflora biodiversity. The results of RAPD-PCR and ERIC-PCR showed that bands under 10 mg · kg^-1 and 50 mg · kg^-1 were the most, and their similarity was the largest. Bands under 500 mg · kg^-1 were the least and similarity with other groups was the minimum. Ileum microflora biodiversity under 10 mg · kg^-1 or 50 mg · kg^-1 was richer than that under 500 mg · kg^-1. A corn-soybean-mixed basal diet supplement with 10 mg · kg^-1 to 50 mg · kg^-1 of daidzein might be beneficial to Hy-Line variety brown layers intestinal bacteria.

Microstructure of Ni /WC Surface Composite Layer on Gray Iron Substrate

The surface infiltrated composite （Ni/WC） layers on gray iron substrate were fabricated through a vacuum infiltration casting technique （VICT） using Ni-based composite powder with different WC particles content as raw materials.The microstructures of surface infiltrated composite layer,the interface structures between surface composite layer and the substrate,the changes of macro-hardness with the increasing of WC content and the micro-hardness distribution are investigated.The infiltrated composite layer includes a surface composite layer and a transition layer,and the thickness of the transition layer decreases with the increasing content of WC.The thickness of transition layer with 20%WC content in the surface infiltrated composite layer was 170 μm which was the thickest for all transition layers with different WC content.The surface composite layer was mainly composed of WC,W2C,FeB and NiB,along with Ni-Cr-Fe,Ni （Cr） solid solution,Ni （Si） solid solution and Ni （Fe） solid solution.The transition layer was composed of Ni （Cr） solid solution,Ni （Fe） solid solution,Ni （Si） solid solution,Fe （Ni） solid solution and eutectic.The surface macro-hardness and micro-hardness of the infiltrated layer had been evaluated.The macro-hardness of the surface composite layer decreases with the WC content increasing,and the average macro-hardness is HRC60.The distribution of micro-hardness presents gradient change.The average micro-hardness of the infiltrated layer is about HV1000.

Analysis of Pressure Forcings for the Vertical Turbulent Fluxes in the Convective Boundary Layer at Gray Zone Resolutions

At kilometer and sub-kilometer resolutions,known as the numerical gray zone for boundary layer turbulence,the atmospheric boundary layer turbulence becomes partially resolved and partially subgrid-scale(SGS) in a numerical model,thus requiring scale-adaptive turbulence schemes.Such schemes are often built by modifying the existing parameterizations,either the planetary boundary layer(PBL) schemes or the large-eddy simulation(LES) closures,to produce the right SGS turbulent fluxes at gray zone resolutions.However,the underlying forcings responsible for the changes in the vertical turbulent fluxes are largely ignored in these approaches.This study follows the original approach of Wyngaard(2004) and analyzes the turbulent buoyancy and momentum flux budgets,to gain a better understanding of the variations of flux forcings at gray zone resolutions.The investigation focuses on the pressure covariance term,which is one of the most dominant terms in the budget equations.By using the coarse-grained LES of a dry convective boundary layer(CBL) case as reference,two widely-used pressure covariance models are evaluated and optimized across the gray zone resolution range.The optimized linear model is further evaluated a priori against another dry CBL case with a different bulk stability,and a shallow-cumulus-topped boundary layer case.The model applies well to both cases,and notably shows good performance for the cloud layer.Based on the analysis of the flux forcings and the optimized pressure model,a scale-adaptive turbulence model for the gray zone is derived from the steady-state flux budgets.