Poor soil is one of the agricultural world’s principal challenges, inciting the use of chemical fertilizer’s to improve overall soil quality. However, the use of chemical fertilizer has significant and cascading env...Poor soil is one of the agricultural world’s principal challenges, inciting the use of chemical fertilizer’s to improve overall soil quality. However, the use of chemical fertilizer has significant and cascading environmental consequences. Therefore, the use of beneficial microbes’ inoculation in treating poor soil is a considerably ecofriendly sustainable solution. In the current study, we supplemented nutrient-deprived soil with plant growth promoting bacteria (PGPB), Pseudomonas fluorescens. The bacterial inoculations of Pseudomonas fluorescenswere added to the poor soil following two days post-sowing of Zea mays var. amylacea and Pennisetumamericanum p. seedlings. Metabolite analyses were conducted two months after treatment for both shoots and roots using nuclear magnetic resonance method (NMR). The data indicated significant changes in 19 metabolites relative to control in both plants shoot and roots. Among these metabolites, 7 were upregulated in roots of Zea mays var. amylacea, and 9 metabolites were upregulated in roots of Pennisetum americanum p. The PGPB enhanced sugars (fructose, glucose, sucrose) and amino acids (glutamate, alanine and succinate) in roots, while down regulating in shoots of Pennisetum americanum p. The Pseudomonas fluorescens induced, predominantly,Aminoacyl-tRNA related metabolite, and Alanine, aspartate and glutamate metabolite biosynthesis in Zea mays var. amylacea), whereas PGPB induced metabolites in Pennisetum americanum p., dominated by up regulated carbohydrate related (starch and sucrose) metabolites. The difference in some metabolic response between the two plants indicated that PGPB influence has a species-specific manner.展开更多
Prospects for deploying perennial grasses that are currently considered leading candidates for dedicated energy crops over large acreages are debatable because of several limitations, including vegetative propagation ...Prospects for deploying perennial grasses that are currently considered leading candidates for dedicated energy crops over large acreages are debatable because of several limitations, including vegetative propagation or small seed size, low biomass production during the first growing season, and incomplete assessments of crop invasiveness risk. Pearl Millet-Napiergrass hybrids (“PMN”;Pennisetum glaucum [L.] R. Br. × P. purpureum Schumach.), in contrast, are large-seeded, sterile feedstocks capable of high biomass production during establishment year. Novel methods are warranted for confirmation of PMN hybrids, as traditional morphological observations can be inconclusive and chromosome number determination using cytological methods is laborious and time consuming. Six putative PMN lines were produced in this study, and 10 progeny from each line were evaluated using morphological traits, seed fertility, flow cytometry, and expressed sequence tag-simple sequence repeat (EST-SSR) markers. All putative hybrid lines were sterile and failed to produce seed. The PMN hybrids could not be distinguished from either parent using flow cytometry due to highly similar nuclear genome DNA contents. A number of paternal napiergrass-specific EST-SSRs were identified for each PMN line, and four paternal-specific EST-SSRs conserved across all napiergrass accessions were selected to screen the putative PMN hybrids. These EST-SSRs confirmed that all F1 individuals analyzed were PMN hybrids. The use of paternal-specific markers therefore provides a valuable tool in the development of both “Seeded-yet-Sterile” biofuel PMN feedstocks and additional PMN cultivar-and parental species-specific markers.展开更多
The plant height,tiller number,yield of three warm-season forage varieties in Southern China were studied for consecutive two years.The result showed that the three varieties all adapted to the environmental condition...The plant height,tiller number,yield of three warm-season forage varieties in Southern China were studied for consecutive two years.The result showed that the three varieties all adapted to the environmental conditions.The annual fresh grass yield of Pennisetum purpureum Schum ev.Taiwan,Guimu-1 hybrid P.purpureum and P.sinese Roxb were 220.85,163.18,186.20 t/hm^2;the crude protein content were 17.39%,19.02%and 20.91%;the crude fat content were 6.37%,5.37%and 4.69%;and the crude fiber content were 33.49%,32.22%and 33.58%,respectively.P.purpureum Schum ev.Taiwan had stronger cold resistance,fast growth after overwintering and strong resistance,and no plant diseases and insect pests were found during the test.P.purpureum Schum ev.Taiwan is a high yield and high quality forage variety,which is worth popularizing and applying.展开更多
文摘Poor soil is one of the agricultural world’s principal challenges, inciting the use of chemical fertilizer’s to improve overall soil quality. However, the use of chemical fertilizer has significant and cascading environmental consequences. Therefore, the use of beneficial microbes’ inoculation in treating poor soil is a considerably ecofriendly sustainable solution. In the current study, we supplemented nutrient-deprived soil with plant growth promoting bacteria (PGPB), Pseudomonas fluorescens. The bacterial inoculations of Pseudomonas fluorescenswere added to the poor soil following two days post-sowing of Zea mays var. amylacea and Pennisetumamericanum p. seedlings. Metabolite analyses were conducted two months after treatment for both shoots and roots using nuclear magnetic resonance method (NMR). The data indicated significant changes in 19 metabolites relative to control in both plants shoot and roots. Among these metabolites, 7 were upregulated in roots of Zea mays var. amylacea, and 9 metabolites were upregulated in roots of Pennisetum americanum p. The PGPB enhanced sugars (fructose, glucose, sucrose) and amino acids (glutamate, alanine and succinate) in roots, while down regulating in shoots of Pennisetum americanum p. The Pseudomonas fluorescens induced, predominantly,Aminoacyl-tRNA related metabolite, and Alanine, aspartate and glutamate metabolite biosynthesis in Zea mays var. amylacea), whereas PGPB induced metabolites in Pennisetum americanum p., dominated by up regulated carbohydrate related (starch and sucrose) metabolites. The difference in some metabolic response between the two plants indicated that PGPB influence has a species-specific manner.
文摘Prospects for deploying perennial grasses that are currently considered leading candidates for dedicated energy crops over large acreages are debatable because of several limitations, including vegetative propagation or small seed size, low biomass production during the first growing season, and incomplete assessments of crop invasiveness risk. Pearl Millet-Napiergrass hybrids (“PMN”;Pennisetum glaucum [L.] R. Br. × P. purpureum Schumach.), in contrast, are large-seeded, sterile feedstocks capable of high biomass production during establishment year. Novel methods are warranted for confirmation of PMN hybrids, as traditional morphological observations can be inconclusive and chromosome number determination using cytological methods is laborious and time consuming. Six putative PMN lines were produced in this study, and 10 progeny from each line were evaluated using morphological traits, seed fertility, flow cytometry, and expressed sequence tag-simple sequence repeat (EST-SSR) markers. All putative hybrid lines were sterile and failed to produce seed. The PMN hybrids could not be distinguished from either parent using flow cytometry due to highly similar nuclear genome DNA contents. A number of paternal napiergrass-specific EST-SSRs were identified for each PMN line, and four paternal-specific EST-SSRs conserved across all napiergrass accessions were selected to screen the putative PMN hybrids. These EST-SSRs confirmed that all F1 individuals analyzed were PMN hybrids. The use of paternal-specific markers therefore provides a valuable tool in the development of both “Seeded-yet-Sterile” biofuel PMN feedstocks and additional PMN cultivar-and parental species-specific markers.
基金Supported by Introduction,Cultivation,Demonstration and Promotion Fund Project of High-quality Forage Grass(2010CY012)
文摘The plant height,tiller number,yield of three warm-season forage varieties in Southern China were studied for consecutive two years.The result showed that the three varieties all adapted to the environmental conditions.The annual fresh grass yield of Pennisetum purpureum Schum ev.Taiwan,Guimu-1 hybrid P.purpureum and P.sinese Roxb were 220.85,163.18,186.20 t/hm^2;the crude protein content were 17.39%,19.02%and 20.91%;the crude fat content were 6.37%,5.37%and 4.69%;and the crude fiber content were 33.49%,32.22%and 33.58%,respectively.P.purpureum Schum ev.Taiwan had stronger cold resistance,fast growth after overwintering and strong resistance,and no plant diseases and insect pests were found during the test.P.purpureum Schum ev.Taiwan is a high yield and high quality forage variety,which is worth popularizing and applying.
