Due to the coaxial connection of engine, motor and pump, the dynamic characteristics of hybrid construction machinery are changed, which generates a new torsional vibration problem of multi-power sources. To reduce th...Due to the coaxial connection of engine, motor and pump, the dynamic characteristics of hybrid construction machinery are changed, which generates a new torsional vibration problem of multi-power sources. To reduce the torsional vibration of the hybrid construction machinery complex shafting, torsional vibration active control was proposed. The three-mass model of coaxial shafting of hybrid construction machinery was established. The PID control and the fuzzy sliding mode control were chosen to weaken torsional vibration by controlling the motor speed and torque. The simulation results show that the fuzzy sliding mode control has 12% overshoot of the PID control when the engine torque changes. The active control is effective and can realize smooth power switch.展开更多
The shift scheduling system of the transmission has an important effect on the dynamic and economic performance of hybrid vehicles. In this work, shift scheduling strategies are developed for parallel hybrid construct...The shift scheduling system of the transmission has an important effect on the dynamic and economic performance of hybrid vehicles. In this work, shift scheduling strategies are developed for parallel hybrid construction vehicles. The effect of power distribution and direction on shift characteristics of the parallel hybrid vehicle with operating loads is evaluated, which must be considered for optimal shift control. A power distribution factor is defined to accurately describe the power distribution and direction in various parallel hybrid systems. This paper proposes a Levenberg-Marquardt algorithm optimized neural network shift scheduling strategy. The methodology contains two objective functions, it is a dynamic combination of a dynamic shift schedule for optimal vehicle acceleration, and an energy-efficient shift schedule for optimal powertrain efficiency. The study is performed on a test bench under typical operating conditions of a wheel loader. The experimental results show that the proposed strategies offer effective and competitive shift performance.展开更多
Objective To construct and screen the suppression subtractive hybridization (SSH) library of human renal cell carcinoma (RCC). Methods Poly A+ RNA was isolated from RCC lines 786-O(tester) and renal cell(RC) lines HK-...Objective To construct and screen the suppression subtractive hybridization (SSH) library of human renal cell carcinoma (RCC). Methods Poly A+ RNA was isolated from RCC lines 786-O(tester) and renal cell(RC) lines HK-2 ( driver), respectiely. SSH procedure was performed according to the protocol of the PCR-Select cDNA Subtraction Kit ( Clontech), and PCR products were cloned into pT-Adv vector and transformed E. coli TOP10F’. All positive clones picked out were digested and some of which were sequenced. Results The SSH library contained 362 clones with SSH cDNA fragments distributed mainly from 0.3 to 0.9 kb. Among 50 clones sequenced randomly,2 represented unknown genes and the other 48 derived from 36 known genes. Conclusion The quality of the SSH library of human RCC is reliable and is construction is the basis for further screening differentially expressed genes of RCC. 6 refs,4 figs, 1 tab.展开更多
The integrated wetland systems were constructed by combining horizontal-flow and vertical-flow bed, and their purification efficiencies for septic tank effluent were detected when the hydraulic retention time (HRT) ...The integrated wetland systems were constructed by combining horizontal-flow and vertical-flow bed, and their purification efficiencies for septic tank effluent were detected when the hydraulic retention time (HRT) was 1 d, 3 d, 5 d under different seasons. The results showed that the removal efficiencies of the organics, phosphorus were steady in the hybrid systems, but the removal efficiency of total nitrogen was not steady due to high total nitrogen concentration in the septic tank effluent. The average removal rates of COD (chemical oxygen demand) were 89%, 87%, 83%, and 86% in summer, autumn, winter and spring, respectively, and it was up to 88%, 85%, 73%, and 74% for BOD5 (5 d biochemical oxygen demand) removal rate in four seasons. The average removal rates of TP (total phosphorous) could reach up to 97%, 98%, 95%, 98% in four seasons, but the removal rate of TN (total nitrogen) was very low. The results of this study also indicated that the capability of purification was the worst in winter. Cultivating with plants could improve the treated effluent quality fTom the hybrid systems. The results of the operation of the horizontal-flow and vertical-flow cells (hybrid systems) showed that the removal efficiencies of the organics, TP and TN in horizontal-flow and vertical-flow cells were improved significantly with the extension of HRT under the same season. The removal rate of 3 d HRT was obviously higher than that of 1 d HRT, and the removal rate of 5 d HRT was better than that of 3 d HRT, but the removal efficiency was not very obvious with the increment of HRT. Therefore, 3 d HRT might be recommended in the actual operation of the hybrid systems for economic and technical reasons.展开更多
In developing countries,high cost of conventional wastewater treatment is a major hindrance in its application.Constructed wetlands(CWs)offer low-cost and effective solution to this issue.The current study aimed to ev...In developing countries,high cost of conventional wastewater treatment is a major hindrance in its application.Constructed wetlands(CWs)offer low-cost and effective solution to this issue.The current study aimed to evaluate an innovative maneuver of CWs i.e.hybrid flow constructed wetlands(HCWs)for municipal wastewater(MWW).The HCWs included two lab scale CWs;one horizontal and one vertical,in series.Local plant species were used.HCWs were operated in both,batch and continuous mode.Batch mode was used to(1)optimize detention time and(2)find pollutants removal efficiency.Continuous operation(at batch optimized retention time)was carried out for the evaluation of mass removal rate,r(g·m-2·d-1),volumetric rate constant,Kv(per day)and areal rate constant,Ka(m·d-1).Among two local plants tested,Pistia stratiotes gave better removal efficiency than Typha.Optimum detention time in HCWs was found to be 8 days(4+4 each).The optimum COD,BOD,TSS,TKN and P removal observed for Pistia stratiotes planted HCWs was 80%,84%,82%,71%and88%respectively.Effluent standards for COD,BOD and TSS were met at optimum conditions.The values of Kaand Kv demonstrated that more removal occurred in vertical flow as compared to horizontal flow CW.展开更多
A molecular biology method, fluorescent in situ hybridization(FISH), in which the pre-treatment was improved in allusion to the media of the constructed wetlands(CW), e.g. the soil and the grit, was used to invest...A molecular biology method, fluorescent in situ hybridization(FISH), in which the pre-treatment was improved in allusion to the media of the constructed wetlands(CW), e.g. the soil and the grit, was used to investigate the vertical distribution characteristics of ammonia-oxidizing bacteria(AOB) quantity and the relation with oxidation-reduction potential(ORP) in the Typha latifolia constructed wetlands under three different Ioadings in summer from May to September. Results showed that the quantity of the AOB decreased in the Typha latifolia CW with the increase of vertical depth. However, the AOB quantity was 2-4 times the quantity of the control in the root area. Additionally, ORP in the rhizosphere was found to be higher than other areas, which showed that Typha latifolia CW was in an aerobic state in summer when using simulated non-point sewage at the rural area of Taihu Lake in China and small town combined sewage.展开更多
Aiming at the flexible flowshop group scheduling problem,taking sequence dependent setup time and machine skipping into account, a mathematical model for minimizing makespan is established,and a hybrid differential ev...Aiming at the flexible flowshop group scheduling problem,taking sequence dependent setup time and machine skipping into account, a mathematical model for minimizing makespan is established,and a hybrid differential evolution( HDE) algorithm based on greedy constructive procedure( GCP) is proposed,which combines differential evolution( DE) with tabu search( TS). DE is applied to generating the elite individuals of population,while TS is used for finding the optimal value by making perturbation in selected elite individuals. A lower bounding technique is developed to evaluate the quality of proposed algorithm. Experimental results verify the effectiveness and feasibility of proposed algorithm.展开更多
Rational manipulation of multi-metal hybrids with controllable compositions and constructions is evolv-ing as an efficacious strategy for tunable electromagnetic(EM)characteristics and EM wave(EMW)ab-sorption.Herein,a...Rational manipulation of multi-metal hybrids with controllable compositions and constructions is evolv-ing as an efficacious strategy for tunable electromagnetic(EM)characteristics and EM wave(EMW)ab-sorption.Herein,a novel bi-orientation etching approach is proposed to regulate the chemical composi-tions and hybridization construction of Zn/Co hybrids,thence meeting the synergistic interaction among diverse compositions for ultra-high EMW absorption.Attributing to the stepwise heterometallic modi-fication by Zn in Co-ZIFs(Zn@Co-ZIF)and Co in Zn-ZIFs(Co@Zn-ZIF),corresponding pyrolysis products with variable Zn/Co atomic concentration and hybridization states(Zn@Co-C,Co@Zn-C)can be obtained.The Co species was confirmed to have a positive impact on both the dielectric and magnetic response,while an appropriate amount of Zn hybridization in Co substance further enhanced the synergistic ef-fect between multiple loss mechanisms,promoting the minimum reflection loss and effective absorption bandwidth of Zn@Co-C reached-34.99 dB and 5.52 GHz(2.00 mm),respectively.Finally,this work re-veals the composition and construction dependency of bimetal hybrids on EMW absorption,which can be a strong reference for developing EMAs with diverse hybridization structures.展开更多
基金Project(51205415)supported by the National Natural Science Foundation of ChinaProject(14JJ3020)supported by the Natural Science Foundation of Hunan Province,China+2 种基金Project(2013M542129)supported by China Postdoctoral Science FoundationProject(2012QNZT014)supported by the Fundamental Research Funds for the Central Universities,ChinaProject supported by the Postdoctoral Foundation of Central South University,China
文摘Due to the coaxial connection of engine, motor and pump, the dynamic characteristics of hybrid construction machinery are changed, which generates a new torsional vibration problem of multi-power sources. To reduce the torsional vibration of the hybrid construction machinery complex shafting, torsional vibration active control was proposed. The three-mass model of coaxial shafting of hybrid construction machinery was established. The PID control and the fuzzy sliding mode control were chosen to weaken torsional vibration by controlling the motor speed and torque. The simulation results show that the fuzzy sliding mode control has 12% overshoot of the PID control when the engine torque changes. The active control is effective and can realize smooth power switch.
基金Project(51805200)supported by the National Natural Science Foundation of ChinaProject(20170520096JH)supported by the Science and Technology Development Plan of Jilin Province,ChinaProject(2016YFC0802900)supported by the National Key R&D Program of China
文摘The shift scheduling system of the transmission has an important effect on the dynamic and economic performance of hybrid vehicles. In this work, shift scheduling strategies are developed for parallel hybrid construction vehicles. The effect of power distribution and direction on shift characteristics of the parallel hybrid vehicle with operating loads is evaluated, which must be considered for optimal shift control. A power distribution factor is defined to accurately describe the power distribution and direction in various parallel hybrid systems. This paper proposes a Levenberg-Marquardt algorithm optimized neural network shift scheduling strategy. The methodology contains two objective functions, it is a dynamic combination of a dynamic shift schedule for optimal vehicle acceleration, and an energy-efficient shift schedule for optimal powertrain efficiency. The study is performed on a test bench under typical operating conditions of a wheel loader. The experimental results show that the proposed strategies offer effective and competitive shift performance.
文摘Objective To construct and screen the suppression subtractive hybridization (SSH) library of human renal cell carcinoma (RCC). Methods Poly A+ RNA was isolated from RCC lines 786-O(tester) and renal cell(RC) lines HK-2 ( driver), respectiely. SSH procedure was performed according to the protocol of the PCR-Select cDNA Subtraction Kit ( Clontech), and PCR products were cloned into pT-Adv vector and transformed E. coli TOP10F’. All positive clones picked out were digested and some of which were sequenced. Results The SSH library contained 362 clones with SSH cDNA fragments distributed mainly from 0.3 to 0.9 kb. Among 50 clones sequenced randomly,2 represented unknown genes and the other 48 derived from 36 known genes. Conclusion The quality of the SSH library of human RCC is reliable and is construction is the basis for further screening differentially expressed genes of RCC. 6 refs,4 figs, 1 tab.
文摘The integrated wetland systems were constructed by combining horizontal-flow and vertical-flow bed, and their purification efficiencies for septic tank effluent were detected when the hydraulic retention time (HRT) was 1 d, 3 d, 5 d under different seasons. The results showed that the removal efficiencies of the organics, phosphorus were steady in the hybrid systems, but the removal efficiency of total nitrogen was not steady due to high total nitrogen concentration in the septic tank effluent. The average removal rates of COD (chemical oxygen demand) were 89%, 87%, 83%, and 86% in summer, autumn, winter and spring, respectively, and it was up to 88%, 85%, 73%, and 74% for BOD5 (5 d biochemical oxygen demand) removal rate in four seasons. The average removal rates of TP (total phosphorous) could reach up to 97%, 98%, 95%, 98% in four seasons, but the removal rate of TN (total nitrogen) was very low. The results of this study also indicated that the capability of purification was the worst in winter. Cultivating with plants could improve the treated effluent quality fTom the hybrid systems. The results of the operation of the horizontal-flow and vertical-flow cells (hybrid systems) showed that the removal efficiencies of the organics, TP and TN in horizontal-flow and vertical-flow cells were improved significantly with the extension of HRT under the same season. The removal rate of 3 d HRT was obviously higher than that of 1 d HRT, and the removal rate of 5 d HRT was better than that of 3 d HRT, but the removal efficiency was not very obvious with the increment of HRT. Therefore, 3 d HRT might be recommended in the actual operation of the hybrid systems for economic and technical reasons.
文摘In developing countries,high cost of conventional wastewater treatment is a major hindrance in its application.Constructed wetlands(CWs)offer low-cost and effective solution to this issue.The current study aimed to evaluate an innovative maneuver of CWs i.e.hybrid flow constructed wetlands(HCWs)for municipal wastewater(MWW).The HCWs included two lab scale CWs;one horizontal and one vertical,in series.Local plant species were used.HCWs were operated in both,batch and continuous mode.Batch mode was used to(1)optimize detention time and(2)find pollutants removal efficiency.Continuous operation(at batch optimized retention time)was carried out for the evaluation of mass removal rate,r(g·m-2·d-1),volumetric rate constant,Kv(per day)and areal rate constant,Ka(m·d-1).Among two local plants tested,Pistia stratiotes gave better removal efficiency than Typha.Optimum detention time in HCWs was found to be 8 days(4+4 each).The optimum COD,BOD,TSS,TKN and P removal observed for Pistia stratiotes planted HCWs was 80%,84%,82%,71%and88%respectively.Effluent standards for COD,BOD and TSS were met at optimum conditions.The values of Kaand Kv demonstrated that more removal occurred in vertical flow as compared to horizontal flow CW.
文摘A molecular biology method, fluorescent in situ hybridization(FISH), in which the pre-treatment was improved in allusion to the media of the constructed wetlands(CW), e.g. the soil and the grit, was used to investigate the vertical distribution characteristics of ammonia-oxidizing bacteria(AOB) quantity and the relation with oxidation-reduction potential(ORP) in the Typha latifolia constructed wetlands under three different Ioadings in summer from May to September. Results showed that the quantity of the AOB decreased in the Typha latifolia CW with the increase of vertical depth. However, the AOB quantity was 2-4 times the quantity of the control in the root area. Additionally, ORP in the rhizosphere was found to be higher than other areas, which showed that Typha latifolia CW was in an aerobic state in summer when using simulated non-point sewage at the rural area of Taihu Lake in China and small town combined sewage.
基金Shanghai Municipal Natural Science Foundation of China(No.10ZR1431700)
文摘Aiming at the flexible flowshop group scheduling problem,taking sequence dependent setup time and machine skipping into account, a mathematical model for minimizing makespan is established,and a hybrid differential evolution( HDE) algorithm based on greedy constructive procedure( GCP) is proposed,which combines differential evolution( DE) with tabu search( TS). DE is applied to generating the elite individuals of population,while TS is used for finding the optimal value by making perturbation in selected elite individuals. A lower bounding technique is developed to evaluate the quality of proposed algorithm. Experimental results verify the effectiveness and feasibility of proposed algorithm.
基金supported by the National Natural Science Foundation of China(No.51407134)Natural Science Foundation of Shandong Province(No.ZR2019YQ24)+1 种基金Taishan Scholars and Young Experts Program of Shandong Province(No.tsqn202103057)the Qingchuang Talents Induction Program of Shandong Higher Education Institution(Research and Innovation Team of Structural-Functional Polymer Composites).
文摘Rational manipulation of multi-metal hybrids with controllable compositions and constructions is evolv-ing as an efficacious strategy for tunable electromagnetic(EM)characteristics and EM wave(EMW)ab-sorption.Herein,a novel bi-orientation etching approach is proposed to regulate the chemical composi-tions and hybridization construction of Zn/Co hybrids,thence meeting the synergistic interaction among diverse compositions for ultra-high EMW absorption.Attributing to the stepwise heterometallic modi-fication by Zn in Co-ZIFs(Zn@Co-ZIF)and Co in Zn-ZIFs(Co@Zn-ZIF),corresponding pyrolysis products with variable Zn/Co atomic concentration and hybridization states(Zn@Co-C,Co@Zn-C)can be obtained.The Co species was confirmed to have a positive impact on both the dielectric and magnetic response,while an appropriate amount of Zn hybridization in Co substance further enhanced the synergistic ef-fect between multiple loss mechanisms,promoting the minimum reflection loss and effective absorption bandwidth of Zn@Co-C reached-34.99 dB and 5.52 GHz(2.00 mm),respectively.Finally,this work re-veals the composition and construction dependency of bimetal hybrids on EMW absorption,which can be a strong reference for developing EMAs with diverse hybridization structures.