Clinical role and importance of fluorescence in situ hybridization method in diagnosis of H pylori infection and determination of clarithromycin resistance in H pylori eradication therapy

H pylori is etiologically associated with gastritis, gas-tric and duodenal ulcers, gastric adenocarcinoma and mucosa-associated lymphoid tissue (MALT) lymphoma. Eradicating H pylori may convert rapidly the outcome of related diseases with the use of more accurate diagnostic molecular tests. Indeed some of the tests cannot give the evidence of current infection; H pylori can be detected by noninvasive and invasive methods, the latter requiring an endoscopy. Eradication failure is a big problem in H pylori infection. Recently, clarithromycin resistance in H pylori strains is increasing and eradicati-on therapy of this bacterium is becoming more difficult. Molecular methods have frequently been applied besides phenotypic methods for susceptibility testing to detect clarithromycin resistance due to mutations in the 2143 and 2144 positions of 23S rRNA gene. Fluorescence in situ hybridization (FISH) method on paraffin embedded tissue is a rapid, accurate and cost-effective method for the detection of H pylori infection and to determine clarithromycin resistance within three hours according to the gold standards as a non-culture method. This method can also be applied to fresh biopsy samples and the isolated colonies from a culture of H pylori, detecting both the culturable bacillary forms and the coccoid forms of H pylori, besides the paraffin embedded tissue secti-ons. This technique is helpful for determining the bac-terial density and the results of treatment where clarith-romycin has been widely used in populations to increase the efficacy of the treatment and to clarify the treatment failure in vitro.

Hybrid Strategy of Partitioned and Monolithic Methods for Solving Strongly Coupled Analysis of Inverse and Direct Piezoelectric and Circuit Coupling

The inverse and direct piezoelectric and circuit coupling are widely observed in advanced electro-mechanical systems such as piezoelectric energy harvesters.Existing strongly coupled analysis methods based on direct numerical modeling for this phenomenon can be classified into partitioned or monolithic formulations.Each formulation has its advantages and disadvantages,and the choice depends on the characteristics of each coupled problem.This study proposes a new option:a coupled analysis strategy that combines the best features of the existing formulations,namely,the hybrid partitioned-monolithic method.The analysis of inverse piezoelectricity and the monolithic analysis of direct piezoelectric and circuit interaction are strongly coupled using a partitioned iterative hierarchical algorithm.In a typical benchmark problem of a piezoelectric energy harvester,this research compares the results from the proposed method to those from the conventional strongly coupled partitioned iterative method,discussing the accuracy,stability,and computational cost.The proposed hybrid concept is effective for coupled multi-physics problems,including various coupling conditions.

Research on the Generation Mechanism and Suppression Method of Aerodynamic Noise in Expansion Cavity Based on Hybrid Method

The expansion chamber serves as the primary silencing structure within the exhaust pipeline.However,it can also act as a sound-emitting structure when subjected to airflow.This article presents a hybrid method for numerically simulating and analyzing the unsteady flow and aerodynamic noise in an expansion chamber under the influence of airflow.A fluid simulation model is established,utilizing the Large Eddy Simulation(LES)method to calculate the unsteady flow within the expansion chamber.The simulation results effectively capture the development and changes of the unsteady flow and vorticity inside the cavity,exhibiting a high level of consistency with experimental observations.To calculate the aerodynamic noise sources within the cavity,the flow field results are integrated using the method of integral interpolation and inserted into the acoustic grid.The acoustic analogy method is then employed to determine the aerodynamic noise sources.An acoustic simulation model is established,and the flow noise source is imported into the sound field grid to calculate the sound pressure at the far-field response point.The calculated sound pressure levels and resonance frequencies show good agreement with the experimental results.To address the issue of airflow regeneration noise within the cavity,perforated tubes are selected as a means of noise suppression.An experimental platformfor airflow regeneration noise is constructed,and experimental samples are processed to analyze and verify the noise suppression effect of perforated tube expansion cavities under different airflow velocities.The research findings indicate that the perforated tube expansion cavity can effectively suppress low-frequency aerodynamic noise within the cavity by impeding the formation of strong shear layers.Moreover,the semi-perforated tube expansion cavity demonstrates the most effective suppression of aerodynamic noise.

A Hybrid Level Set Optimization Design Method of Functionally Graded Cellular Structures Considering Connectivity

With the continuous advancement in topology optimization and additive manufacturing(AM)technology,the capability to fabricate functionally graded materials and intricate cellular structures with spatially varying microstructures has grown significantly.However,a critical challenge is encountered in the design of these structures–the absence of robust interface connections between adjacent microstructures,potentially resulting in diminished efficiency or macroscopic failure.A Hybrid Level Set Method(HLSM)is proposed,specifically designed to enhance connectivity among non-uniform microstructures,contributing to the design of functionally graded cellular structures.The HLSM introduces a pioneering algorithm for effectively blending heterogeneous microstructure interfaces.Initially,an interpolation algorithm is presented to construct transition microstructures seamlessly connected on both sides.Subsequently,the algorithm enables the morphing of non-uniform unit cells to seamlessly adapt to interconnected adjacent microstructures.The method,seamlessly integrated into a multi-scale topology optimization framework using the level set method,exhibits its efficacy through numerical examples,showcasing its prowess in optimizing 2D and 3D functionally graded materials(FGM)and multi-scale topology optimization.In essence,the pressing issue of interface connections in complex structure design is not only addressed but also a robust methodology is introduced,substantiated by numerical evidence,advancing optimization capabilities in the realm of functionally graded materials and cellular structures.

ACTIVATION OF NUCLEOLAR rRNA GENE TRANSCRIPTION IN LYMPHOCYTES BY TUMOR PROMOTERS: APPLIES STUDIES WITH IN SITU HYBRIDIZATION DETECTED BY FLUORESCENT METHOD

The distribution of rDNA was visualized in interphase nuclei of tumor promoter treated human lymphocytes in comparison with the mltogen Phytoheamagglutinin (PHA) effects by using an in situ hybridization fluorescent method. The procedure involves biotinylated rDNA as the probe and FITC-avidin as detection system. Silver (Ag) staining was used to visualize nucleoli. In the interphase nuclei of most of the nonstimulated control lymphocytes, only one small Ag-stained nucleolus could be seen. The in situ hybridization, however, revealed one to several agglomerations of rDNA fluorescent spots. With tumor promoting herb extract WCE (40 μg/ml) or TPA (60 ng/ ml) treatment, the Interphase nucleoli increased slightly in number with the morphology alteration into larger, reticular or compact granular types. Ag-stained particles also increased in number. The number of the in situ hybridization rDNA fluorescent spots and dots increased markedly and largereticulate formations of numerous rDNA spots were seen. This phenomenum resembles to the changes in PHA stimulated lymphocytes. Statistic analysized data showed signifcant difference between control and drug- treated cells. These results indicate that transcriptionally activated rDNA and amplification of total rDNA was induced by the used tumor promoters.

Behavioral Decision-Making of Key Stakeholders in Public-Private Partnerships:A Hybrid Method and Benefit Distribution Study

Public-private partnerships(PPPs)have been used by governments around the world to procure and construct infrastructural amenities.It relies on private sector expertise and funding to achieve this lofty objective.However,given the uncertainties of project management,transparency,accountability,and expropriation,this phenomenon has gained tremendous attention in recent years due to the important role it plays in curbing infrastructural deficits globally.Interestingly,the reasonable benefit distribution scheme in a PPP project is related to the behavior decisionmaking of the government and social capital,aswell as the performance of the project.In this paper,the government and social capital which are the key stakeholders of PPP projects were selected as the research objects.Based on the fuzzy expected value model and game theory,a hybrid method was adopted in this research taking into account the different risk preferences of both public entities and private parties under the fuzzy demand environment.To alleviate the problem of insufficient utilization of social capital in a PPP project,this paper seeks to grasp the relationship that exists between the benefit distribution of stakeholders,their behavioral decision-making,and project performance,given that they impact the performance of both public entities and private parties,as well as assist in maximizing the overall utility of the project.Furthermore,four game models were constructed in this study,while the expected value and opportunity-constrained programming model for optimal decision-making were derived using alternate perspectives of both centralized decision-making and decentralized decision-making.Afterward,the optimal behavioral decision-making of public entities and private parties in four scenarios was discussed and thereafter compared,which led to an ensuing discussion on the benefit distribution system under centralized decision-making.Lastly,based on an example case,the influence of different confidence levels,price,and fuzzy uncertainties of PPP projects on the equilibrium strategy results of both parties were discussed,giving credence to the effectiveness of the hybrid method.The results indicate that adjusting different confidence levels yields different equilibriumpoints,and therefore signposts that social capital has a fair perception of opportunities,as well as identifies reciprocal preferences.Nevertheless,we find that an increase in the cost coefficient of the government and social capital does not inhibit the effort of both parties.Our results also indicate that a reasonable benefit distribution of PPP projects can assist them in realizing optimum Pareto improvements over time.The results provide us with very useful strategies and recommendations to improve the overall performance of PPP projects in China.

An Improved Preisach Distribution Function Identification Method Considering the Reversible Magnetization

This paper presents an identification method of the scalar Preisach model to consider the effect of reversible magnetization in the process of distribution function identification.By reconsidering the identification process by stripping the influence of reversible components from the measurement data,the Preisach distribution function is identified by the pure irreversible components.In this way,the simulation accuracy of both limiting hysteresis loops and the inner internal symmetrical small hysteresis loop is ensured.Furthermore,through a discrete Preisach plane with a hybrid discretization method,the irreversible magnetic flux density components are computed more efficiently through the improved Preisach model.Finally,the proposed method results are compared with the traditional method and the traditional method considering reversible magnetization and validated by the laboratory test for the B30P105 electrical steel by Epstein frame.

2D multi-scale hybrid optimization method for geophysical inversion and its application

Local and global optimization methods are widely used in geophysical inversion but each has its own advantages and disadvantages. The combination of the two methods will make it possible to overcome their weaknesses. Based on the simulated annealing genetic algorithm （SAGA） and the simplex algorithm, an efficient and robust 2-D nonlinear method for seismic travel-time inversion is presented in this paper. First we do a global search over a large range by SAGA and then do a rapid local search using the simplex method. A multi-scale tomography method is adopted in order to reduce non-uniqueness. The velocity field is divided into different spatial scales and velocities at the grid nodes are taken as unknown parameters. The model is parameterized by a bi-cubic spline function. The finite-difference method is used to solve the forward problem while the hybrid method combining multi-scale SAGA and simplex algorithms is applied to the inverse problem. The algorithm has been applied to a numerical test and a travel-time perturbation test using an anomalous low-velocity body. For a practical example, it is used in the study of upper crustal velocity structure of the A＇nyemaqen suture zone at the north-east edge of the Qinghai-Tibet Plateau. The model test and practical application both prove that the method is effective and robust.

An Innovative Strategy for Reciprocal Distant Hybridization between Spartina alterniflora and Rice

[Objective] This study aimed to explore germplasm resources of salt toler- ant Spartina alterniflora and high yield rice, and develop an innovative strategy for distant hybridization between S. altemiflora and rice. [Method] Crossing （rice ♀ ）： hot water emasculation and glume-cutting pollination method, heat emasculation and pollen-suction pollination method; reciprocal crossing （S. alterniflora ♀ glume-cutting emasculation and pollination method, continuous emasculation-pollination method, hot water emasculation and continuous emasculation-pollination method. [Result] In crossing groups （rice ♀ × S. alterniflora ♂ ）, compared with hot water emasculation and glume-cutting pollination treatment, the number of seed-bearing combinations, seed number per spike and seedling emergence rate in heat emasculation and pollen-suction pollination treatment were improved by 34.09%, 121.21% and 60.07%, respectively; hybridization efficiency was improved by 6-7 times. In reciprocal cross- ing groups （S. altemiflora ♀× rice ♂ ）, compared with glume-cutting emasculation and pollination treatment, the number of seed-bearing combinations and seed num- ber per spike in continuous emasculation-pollination treatment were improved by 3.14 and 4.21 times, respectively; seedling emergence rate was improved by 68.47%; hybridization efficiency was improved by 7-8 times. [Conclusion] Heat emas- culation and pollen-suction pollination method is suitable for hybridization between rice ♀× S. alterniflora ♂; continuous emasculation-pollination method is suitable for hybridization between S. alterniflora ♀× rice ♂.

Hybrid method for investigation of electromagnetic scattering from conducting target above the randomly rough surface

A current based hybrid method （HM） is proposed which combines the method of moment （MOM） with the Kirchhoff approximation （KA） for the analysis of scattering interaction between a two-dimensional （2D） infinitely long conducting target with arbitrary cross section and a one-dimensional （1D） Gaussian rough surface. The electromagnetic scattering region in the HM is split into KA region and MOM region. The electric field integral equation （EFIE） in MOM region （target） is derived, the computational time of the HM depends mainly on the number of unknowns of the target. The bistatic scattering coefficient for the infinitely long cylinder above the rough surface with Gaussian roughness spectrum is calculated, and the numerical results are compared and verified with those obtained by the conventional MOM, which shows the high efficiency of the HM. Finally, the influence of the size, location of the target, the rms height and correlation length of the rough surface on the bistatic scattering coefficient with different polarizations is discussed in detail.

Optimization of mining method in subsea deep gold mines: A case study

The mining method optimization in subsea deep gold mines was studied. First, an index system for subsea mining method selection was established based on technical feasibility, security status, economic benefit, and management complexity. Next, an evaluation matrix containing crisp numbers and triangular fuzzy numbers(TFNs) was constructed to describe quantitative and qualitative information simultaneously. Then, a hybrid model combining fuzzy theory and the Tomada de Decis?o Interativa Multicritério(TODIM) method was proposed. Finally, the feasibility of the proposed approach was validated by an illustrative example of selecting the optimal mining method in the Sanshandao Gold Mine(China). The robustness of this approach was demonstrated through a sensitivity analysis. The results show that the proposed hybrid TODIM method is reliable and stable for choosing the optimal mining method in subsea deep gold mines and provides references for mining method optimization in other similar undersea mines.

Refracturing candidate selection for MFHWs in tight oil and gas reservoirs using hybrid method with data analysis techniques and fuzzy clustering

The selection of refracturing candidate is one of the most important jobs faced by oilfield engineers. However, due to the complicated multi-parameter relationships and their comprehensive influence, the selection of refracturing candidate is often very difficult. In this paper, a novel approach combining data analysis techniques and fuzzy clustering was proposed to select refracturing candidate. First, the analysis techniques were used to quantitatively calculate the weight coefficient and determine the key factors. Then, the idealized refracturing well was established by considering the main factors. Fuzzy clustering was applied to evaluate refracturing potential. Finally, reservoirs numerical simulation was used to further evaluate reservoirs energy and material basis of the optimum refracturing candidates. The hybrid method has been successfully applied to a tight oil reservoir in China. The average steady production was 15.8 t/d after refracturing treatment, increasing significantly compared with previous status. The research results can guide the development of tight oil and gas reservoirs effectively.

AN ANALYSIS OF THE MODELING OF STRESS AND STRAIN FIELDS IN A REAL MICROSTRUC-TURE USING AHYBRID METHOD

The distribution of stress and strain fields in a micro-structuralarea of a particle reinforced composite is studied by a combinationof experimental and numerical method (hybrid method). With the ex-perimental values of displacements in a micro-region as the boundaryloading condition, strain and stress fields inside the micro-regionare calculated by the finite element method under tow different kindsof model- ing, namely, as pale stress and plane strain condition. Thedifferences between the two kinds of modeling conditions as appliedto micro-structural areas are discussed.

DUAL RECIPROCITY HYBRID BOUNDARY NODE METHOD FOR THREE-DIMENSIONAL ELASTICITY WITH BODY FORCE

Combining Dual Reciprocity Method （DRM） with Hybrid Boundary Node Method （HBNM）, the Dual Reciprocity Hybrid Boundary Node Method （DRHBNM） is developed for three-dimensional linear elasticity problems with body force. This method can be used to solve the elasticity problems with body force without domain integral, which is inevitable by HBNM. To demonstrate the versatility and the fast convergence of this method, some numerical examples of 3-D elasticity problems with body forces are examined. The computational results show that the present method is effective and can be widely applied in solving practical engineering problems.

Hybrid Method to Analyze Contact Stress Distribution on Dry Friction Interfaces

A hybrid method is established by combining photoelastic experiment and finite element analysis.The method is used to evaluate contact stress distribution on dry friction interfaces,such as the contact interfaces between shrouds of fan blades and turbine blades.The photoelastic stress frozen experiment method is used to decide the displacement boundary conditions of numerical calculation.Higher accuracy and efficiency of solving problems are improved by the method.Technical difficulty and high cost of experiment are also avoided by the method.Good agreement of the stress distribution by using the hybrid method and experiment is obtained.

Thermal stress analysis method considering geometric effect of risers in sand mold casting process

Solidif ication and f luid f low analysis using computer simulation is a current common practice. There is also a high demand for thermal stress analysis in the casting process because casting engineers want to control the defects related to thermal stresses, such as large deformation and crack generation during casting. The riser system is an essential part of preventing the shrinkage defects in the casting process, and it has a great inf luence on thermal phenomena. The analysis domain is dramatically expanded by attaching the riser system to a casting product due to its large volume, and it makes FEM mesh generation diff icult. However, it is diff icult to study and solve the above proposed problem caused by riser system using traditional analysis methods which use single numerical method such as FEM or FDM. In this paper, some research information is presented on the effects of the riser system on thermal stress analysis using a FDM/FEM hybrid method in the casting process simulation. The results show the optimal conditions for stress analysis of the riser model in order to save computation time and memory resources.

A constrained particle dynamics for continuum-particle hybrid method in micro- and nano-fluidics

A hybrid method of continuum and particle dynamics is developed for micro- and nano-fluidics, where fluids are described by a molecular dynamics （MD） in one domain and by the Navier-Stokes （NS） equations in another domain. In order to ensure the continuity of momentum flux, the continuum and molecular dynamics in the overlap domain are coupled through a constrained particle dynamics. The constrained particle dynamics is constructed with a virtual damping force and a virtual added mass force. The sudden-start Couette flows with either non-slip or slip boundary condition are used to test the hybrid method. It is shown that the results obtained are quantitatively in agreement with the analytical solutions under the non-slip boundary conditions and the full MD simulations under the slip bound- ary conditions.

High performance corrosion and wear resistant composite titanium nitride layers produced on the AZ91D magnesium alloy by a hybrid method

Composite,diffusive titanium nitride layers formed on a titanium and aluminum sub-layer were produced on the AZ91D magnesium alloy.The layers were obtained using a hybrid method which combined the PVD processes with the final sealing by a hydrothermal treatment.The microstructure,resistance to corrosion,mechanical damage,and frictional wear of the layers were examined.The properties of the AZ91D alloy covered with these layers were compared with those of the untreated alloy and of some engineering materials such as 316L stainless steel,100Cr6 bearing steel,and the AZ91D alloy subjected to commercial anodizing.It has been found that the composite diffusive nitride layer produced on the AZ91D alloy and then sealed by the hydrothermal treatment ensures the corrosion resistance comparable with that of 316L stainless steel.The layers are characterized by higher electrochemical durability which is due to the surface being overbuilt with the titanium oxides formed,as shown by the XPS examinations,from titanium nitride during the hydrothermal treatment.The composite titanium nitride layers exhibit high resistance to mechanical damage and wear,including frictional wear which is comparable with that of 100Cr6 bearing steel.The performance properties of the AZ91D magnesium alloy covered with the composite titanium nitride coating are substantially superior to those of the alloy subjected to commercial anodizing which is the dominant technique employed in industrial practice.

IMPROVED GRADIENT METHOD FOR MONOTONE AND LIPSCHITZ CONTINUOUS MAPPINGS IN BANACH SPACES

Let C be a nonempty closed convex subset of a 2-uniformly convex and uniformly smooth Banach space E and {An}n∈N be a family of monotone and Lipschitz continuos mappings of C into E＊. In this article, we consider the improved gradient method by the hybrid method in mathematical programming [i0] for solving the variational inequality problem for {AN} and prove strong convergence theorems. And we get several results which improve the well-known results in a real 2-uniformly convex and uniformly smooth Banach space and a real Hilbert space.