甲壳素水解蛋白对肉仔鸡生长和氮存留率的影响

研究甲壳素水解蛋白对肉仔鸡生长发育和氮存留率的影响,选取初生AA雏鸡360只,采用单因子重复试验设计,甲壳素水解蛋白添加水平分别为0,1%,2%,3%.结果表明添加2%～3%的甲壳素水解蛋白,可显著提高(P＜0.05)肉仔鸡的日均增重,添加1%～2%的甲壳素水解蛋白,可显著改善(P＜0.05)肉仔鸡的料重比及显著提高(P＜0.05)肉仔鸡的氨存留率.

甲壳素水解蛋白对肉仔鸡生长和氮存留率的影响

选取初生AA幼雏 360羽 ,随机分为 4组 ,每组 3个重复 ,每组随机接受一种试验日粮 ,采用单因子试验设计 ,甲壳素水解蛋白的添加水平分别为 0 % ,1 % ,2 % ,3 % ,经过 4周的试验 ,测定日粮中添加不同甲壳素水解蛋白对肉仔鸡生长发育和ARN %的影响。研究结果表明添加2 %～ 3 %的甲壳素水解蛋白 ,可显著提高肉仔鸡的ADG (P <0 0 5) ,添加 1 %～ 2 %的甲壳素水解蛋白 ,可显著改善肉仔鸡的F/G (P <0 0 5)及显著提高肉仔鸡的ARN % (P <0 0 5)。综合考虑各种因素 ,在肉仔鸡基础日粮中CP水平为 2 0 %时 ,甲壳素水解蛋白的适宜添加水平为 2 %。

Influence of Amino Acids on the Secondary Somatic Embryogenesis Proliferation Process of Moroccan Cork Oak (<i>Quercus suber</i>L.)

The present study aims to explore the regeneration potential of Moroccan cork oak through the secondary somatic embryogenesis process. Particularly, we focus on the analysis of amino acids influence on the quantity and quality of the regenerated secondary embryos. The amino acids tested are: Glutamine, asparagine, arginine, tryptophane, methionine, casein hydrolysate and urea. Each amino acid is added in the Margara (N30K) medium at different concentrations ranged between 10 and 500 mg/l. The results are collected after 2 months of culture. First analysis shows that the glutamine and the casein hydrolysate gives a maximum number of somatic embryos, clusters and pre-embryos newly formed on the clusters. By comparison to the control medium, the increase of the secondary embryos number directly formed exceed 36% in the case of casein hydrolysate and 35% of in the case of glutamine both at the concentration of 30 mg/l. However, the test of the combination of these amino acids did not have any significant results. In terms of quality, the influence of amino acids on the morphology of secondary embryos was analyzed.

超高压协同酶法条件下不同分子量大豆分离蛋白水解肽乳化性及抗氧化性研究

目的研究不同分子量分布对大豆分离蛋白水解肽乳化性及抗氧化性的影响。方法采用超滤技术将超高压协同酶解制备的大豆分离蛋白水解物分离得到5个分子量肽段,即分子量(molecular weight,Mw)<3500 u、3500 u<Mw<7000 u、7000 u<Mw<10000 u、10000 u<Mw<20000 u和Mw>20000 u。通过测定乳化性、乳化稳定性、电势、粒径、还原力、以及对2,2-联苯基-1-苦基肼基(2,2-diphenyl-1-picrylhydrazyl,DPPH)和2,2’-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐[2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS]自由基清除率,分析不同分子量水解肽的乳化性及抗氧化性变化。结果7000 u<Mw<10000 u的水解肽具有较高的乳化性、电势和较小的粒径,而10000 u<Mw<20000 u的水解肽则表现出较优的乳化稳定性,但与7000 u<Mw<10000 u的组分差异不显著(P>0.05);水解肽的抗氧化性随肽段分子量的减小呈逐渐增大趋势,且不同分子量肽段与还原力和DPPH自由基清除能力均呈极显著负相关性(P<0.01),与ABTS自由基清除能力呈显著负相关性(P<0.05)。结论超高压协同酶法制备的大豆分离蛋白水解肽的乳化性和抗氧化性与其分子量分布有密切关联,这为相关功能性产品的开发提供了较为切实的理论依据。