栽培基质与EM菌剂对卷叶湿地藓生长的影响研究

为探索卷叶湿地藓的快速扩繁方法,以卷叶湿地藓为研究对象,采用二因素裂区试验设计,设置4种栽培基质和3种EM菌剂浓度,综合分析和评价栽培基质和EM菌剂浓度对卷叶湿地藓生长及生理指标的影响。结果表明:卷叶湿地藓的发枝数、盖度、叶绿素和可溶性糖含量表现为随EM菌剂浓度增高而增加,丙二醛(MDA)随EM菌剂浓度增高而降低。高浓度处理A3B2的发枝数是低浓度处理A3B1的1.16倍,是对照处理A3CK的1.88倍,A3B2处理盖度、叶绿素和可溶性糖含量较对照处理A3CK相比分别增加了57.34%、67.95%和16.94%,较低浓度处理A3B1分别增加了17.30%、29.41%和3.32%,MDA含量较对照A3CK和低浓度处理A3B1分别降低了70.54%和31.56%。园林废弃物堆肥基质处理的发枝数、盖度、叶绿素和可溶性糖含量均略高于草炭基质,A3B2的发枝数、盖度、叶绿素和可溶性糖含量较A4B2处理增加了3.33%、3.83%、4.68%和12.85%,MDA含量较A4B2处理降低了17.47%。隶属函数综合评价结果显示A3B2综合评分最高。综上,栽培基质和EM菌剂对卷叶湿地藓的生长有一定的影响,较适宜卷叶湿地藓的栽培基质和EM菌剂组合为工程弃土40%+园林废弃物堆肥60%配施EM菌剂600倍稀释液。

Qualitative and quantitative phytochemical analysis of selected mosses with different alcoholic solvents

This study was conducted to investigate the qualitative and quantitative phytochemical content of the crude extracts of Archidium ohioense,Pelekium gratum,and Hyophila involuta with different alcoholic solvents(ethanol,methanol,Seaman's Schnapps,fresh oil-palm wine,and fresh Raffia-palm wine).The mosses were collected from their natural populations on the central campus of the Obafemi Awolowo University,Ile-Ife,Nigeria.The yield of the extracts was weighed for all the solvents,and the qualitative and quantitative evaluations of the extracts were carried out using standard methods.The results of phytochemical analysis of the crude extracts from the mosses showed the presence of saponins,cardiac glycosides,triterpenes,alkaloids,flavonoids,and steroids.The quantitative phytochemical analysis of the crude extracts showed that ethanolic extracts of Hyophila involuta had the highest flavonoid content(288.37±0.10 mg RE/g),and Raffiapalm-wine extracts of Hyophila involuta had the highest saponin content(224.70±0.02 mg/g),while the methanolic extract of Archidium ohioense had the highest cardiac glycosides content(63.71±0.14 mg/g),and the Raffia-palm wine extract of Hyophila involuta had the highest alkaloids content(102.50±0.12 mg/g).Raffia-and oil-palm wines were observed to be the most effective solvents for all the mosses studied,followed by Seaman's Schnapp,while methanol and ethanol were less effective.The study concluded that the extracts of the mosses studied contain pharmacologically active constituents that can be used for therapeutic purposes.