Genetic investigation of the ubiquitin-protein ligase E3A gene as putative target in Angelman syndrome

BACKGROUND Angelman syndrome(AS)is caused by maternal chromosomal deletions,imprinting defects,paternal uniparental disomy involving chromosome 15 and the ubiquitin-protein ligase UBE3A gene mutations.However the genetic basis remains unclear for several patients.AIM To investigate the involvement of UBE3A gene in AS and identifying new potential genes using exome sequencing.METHODS We established a cohort study in 50 patients referred to Farhat Hached University Hospital between 2006 and 2021,with a strong suspicion of AS and absence of chromosomal aberrations.The UBE3A gene was screened for mutation detection.Two unrelated patients issued from consanguineous families were subjected to exome analysis.RESULTS We describe seven UBE3A variants among them 3 none previously described including intronic variants c.2220+14T>C(intron14),c.2507+43T>A(Exon15)and insertion in Exon7:c.30-47_30-46.The exome sequencing revealed 22 potential genes that could be involved in AS-like syndromes that should be investigated further.CONCLUSION Screening for UBE3A mutations in AS patients has been proven to be useful to confirm the diagnosis.Our exome findings could rise to new potential alternative target genes for genetic counseling.

Association of Apolipoprotein E Gene Polymorphism with Lipid Profile in Patients with Acute Coronary Syndrome in Han Chinese: A Critical Review

This review paper focuses on the genetic contribution, in particular, the association of Apolipoprotein E gene polymorphism to lipid abnormality and subsequent acute coronary syndrome in Han Chinese of China. Many researches have been published pertaining the influence of ApoE gene polymorphism on coronary artery disease, dyslipidemia and the response of statin in Han Chinese. Most of the studies in Han Chinese like other populations demonstrated that ApoE 4 allele genetically predisposes coronary artery disease, acute coronary syndrome, severity of occlusion of coronary artery and higher incidence of major adverse cardiovascular events (In Han Chinese, ApoE allele carriers demonstrated 85% increase in major adverse cardiovascular events (MACE) in six months follow up). In addition, ApoE4 allele carrier also showed both increased in LDL level and decrease response to statin therapy in dyslipidemic Han Chinese. On the other hand, ApoE2 carrier is scavenger of cholesterol and triglyceride from the blood;?thus it is cardiovascular-protective. Despite positive relationship between ApoE gene polymorphism and cardiovascular pathologies, prognostic outcome and resistance to intervention, this area of research still requires?extensive investigation in Han Chinese. Because, several other studies revealed either negative effect or showed no effect by ApoE gene polymorphism on cardiovascular disease. Some of the causes of such debatable results could be explained by factors such as diminutive frequency allele and expression of ApoE gene in coronary heart disease. This part of the research yet requires extensive study with bulkier sample size and retrospective in nature, in order to ascertain the influence of ApoE genotype on lipid, anti-hyperlipidemic agent and coronary heart disease. Such studies could assist us to confirm whether to test healthier subjects to predict genetic risk of coronary heart disease in Han Chinese population. The aim of this review paper is to critically analyze the effect of ApoE gene on the occurrence of coronary heart disease in Han Chinese.

Guillain-Barrésyndrome following hepatitis E

Guillain-Barrésyndrome(GBS)is often triggered by a preceding bacterial or viral infection.Occasionally,it has been observed in association with acute hepatitis A,B and C,and three cases have been previously described in India in which GBS was associated with acute hepatitis E.A molecular mimicry mechanism is supposed to be involved in the pathogenesis of GBS triggered by infectious agents,although the nature of the shared epitopes has not been characterized in most instances,including that in the case of hepatotropic viruses.We report a case of GBS following acute hepatitis E in a European individual.The presence of antiganglioside GM2 antibodies in this patient suggested molecular mimicry involving ganglioside GM2 in the pathogenesis of GBS associated with hepatitis E.

维生素E对围绝经期和绝经后妇女健康的影响

随着年龄的增长,卵巢功能由旺盛期到逐渐减退,直至衰竭,女性也将从生殖期逐渐进入老年期。这不仅会对月经周期和生育能力有影响,还会伴随着一系列身体和心理上的变化,严重时会影响生活和工作。研究发现卵巢卵泡的发育以及绝经前后出现的泌尿生殖功能异常、骨质疏松、神经认知功能障碍及心血管病变等疾病的发生发展可能与氧化应激反应有着密不可分的关系。维生素E具有抗氧化特性和稳定细胞的能力,已经被证实可在代谢综合征、骨质疏松、心脑血管、神经系统等疾病的预防和治疗方面发挥着重要作用,并且发现维生素E在一定程度上还具有模拟雌激素的作用。因此,补充维生素E对围绝经期和绝经后妇女的健康具有潜在的益处。综述维生素E对围绝经期卵巢卵泡发育、雌激素的影响和绝经综合征的预防和治疗作用,为维生素E作为改善围绝经期和绝经后妇女身心健康的辅助或替代治疗方案提供参考依据。

载脂蛋白E基因多态性与急性冠状动脉综合征关系的研究进展

急性冠状动脉综合征(ACS)是由不稳定的冠状动脉粥样硬化斑块(AP)破裂或糜烂导致血栓形成而引发的一系列缺血性疾病,其病理基础为AP形成,这一过程受到脂质代谢紊乱的驱动。血脂水平是ACS预后的关键因素,载脂蛋白E(ApoE)作为脂质代谢的关键调控因子,其多态性与血脂异常密切相关。而ApoE4基因型患者ACS后强化他汀治疗效果不佳是ACS的独立危险因素。未来明确ApoE基因多态性与ACS的关系,有助于为ACS患者制订个体化降脂方案提供更为精准、安全的策略。

柴郁清肝汤治疗非酒精性脂肪性肝病肝郁脾虚证患者的疗效观察及对Th17/Treg和相关炎症因子的影响

目的:观察柴郁清肝汤治疗非酒精性脂肪性肝病(NAFLD)肝郁脾虚证患者的临床疗效及对外周血Th17/Treg和相关炎症因子的影响。方法:将60例NAFLD患者随机分为观察组(30例)和对照组(30例),其中观察组给予柴郁清肝汤治疗,对照组给予维生素E软胶囊治疗,治疗8周评价疗效;分别观察2组患者治疗前后肝功、血脂、外周血T淋巴细胞Th17、Treg的百分比及其相关炎症因子(IL-17、IL-22、IL-10)的变化。结果:治疗8周,观察组显效16例,有效12例,对照组显效8例,有效13例,2组比较差异具有统计学意义(P<0.05)。与治疗前比较,2组患者ALT、AST、GGT、ALP、TC、TG、LDL-C、Th17、IL-17和IL-22明显降低,Treg明显升高,差异具有统计学意义(P<0.05),2组间比较观察组均明显优于对照组(P<0.05)。结论:柴郁清肝汤能够有效治疗NAFLD患者,改善肝功能和血脂,调节Th17/Treg平衡及其相关炎性因子分泌是其作用机制之一。

Effects of Soy Isoflavones on the Sex Hormone Level and Pathological Changes in the Perimenopausal Syndrome Animal Model

[Objectives] To observe the effect of soy isoflavones on the perimenopausal syndrome model of female rats,and to find its mechanism,in order to provide experimental evidence for the clinical treatment of menopausal syndrome. [Methods]50 female SD rats were randomly divided into five groups,with 10 rats in each group,respectively,for the normal control group,sham operation group,model group,diethylstilbestrol group,soy isoflavone treated group,except the normal control group and sham operation group,the other groups were ovariectomized rats,the formation model of perimenopausal rats treated with normal saline,diethylstilbestrol,soy isoflavone by gavage,normal control group and sham operation group were given the same dose of normal saline and soy isoflavone by gavage. After 6 weeks of continuous treatment,abdominal aorta was taken blood,the serum was isolated,and radioimmunoassay method to measure E_2( Eestradiol),P( Progesterone),T( Testosterone). And removal of the pituitary gland,uterine and adrenal prepared pathological section,experimental effect was observed by light microscopy,using immunohistochemical method to observe the brain pituitary FSH( Follicle-Stimulating Hormone) and LH( Luteinizing Hormone) levels. [Results] Radiation immune analysis results showed that soy isoflavone could significantly improve serum E_2 level in ovariectomized rats,compared with the model group,the statistical analysis showed significant difference( P < 0. 05),but the serum P and T level had little effect. The pathological results showed that( i) uterus: the uterine wall and endometrial thickness of soy isoflavone group was between that of model group and sham operation group,the surface epithelium was columnar,glands increased;( ii) pituitary gland: soy isoflavone ovariectomized group cells were between model group and normal group;( iii) adrenal gland: soy isoflavone group adrenal tissue did not change significantly. Immunohistochemical results showed that soy isoflavone group pituitary tissues of FSH and LH cells compared with the model group decreased significantly. [Conclusions] The soy isoflavones could make the uterus wall and endometria of perimenopausal syndrome animal model thicken,improve the serum level of E_2 in ovariectomized rats,and decrease the level of FSH and LH,but had little effect on the adrenal tissues,level of T and P.

Assessment of antioxidant status of women with polycystic ovarian syndrome

Objective:To determine the antioxidant status of females with polycystic ovarian syndrome.Methods:Blood samples of 85 females(45 newly diagnosed polycystic ovarian syndrome patients and 40 apparently healthy subjects)between 25 and 45 years of age were obtained from Ekiti State University Teaching Hospital,Ado Ekiti,Ekiti State,Nigeria.Spectrophotometry was used to evaluate the plasma concentrations of malondialdehyde,vitamin A,C and E,reduced glutathione and activities of glutathione peroxidase,catalase and superoxide dismutase.Results:The concentrations of malondialdehyde and glutathione peroxidase activity significantly increased(P<0.05)in patients with polycystic ovarian syndrome compared with the healthy controls,while concentrations of reduced glutathione,vitamins A,C,E and activities of catalase and superoxide dismutase of patients with polycystic ovarian syndrome showed significant decrease(P<0.05)compared to the healthy controls.This study showed that oxidative stress may assume a role in the pathogenesis of polycystic ovarian syndrome.There were significant negative correlations between malondialdehyde and superoxide dismutase,catalase(P<0.01)and vitamin A(P<0.05),while there was a significant positive correlation(P<0.01)between malondialdehyde and glutathione peroxidase.In addition,vitamins A(P<0.05),C(P<0.01)and E(P<0.01)showed significant positive correlations with catalase antioxidant enzyme.However,vitamins C and E showed significant positive correlation(P<0.05)among each other.Conclusions:The involvement of antioxidants in the management of polycystic ovarian syndrome may be helpful as secondary therapy to prevent oxidative damage and may be used as a potential approach to overcome metabolic as well as reproductive disorders associated with infertility in polycystic ovarian syndrome.

The Study of Apolipoprotein E4 Allele Distribution in Parents of Down’s Syndrome Children as a Risk Factor in Khorasan Razavi Province, Iran

Backgrounds: Down syndrome (DS) is the most common chromosomal abnormality. The most important factor in DS is increased maternal age so after the age of 35, the risk of Down syndrome in pregnancy increases. Down syndrome can be diagnosed during pregnancy by prenatal screening. Nondisjunction in cell divisions is the main cause of the DS. Apo lipoprotein E is a 317 amino acid glycoprotein that plays an essential role in metabolism and cholesterol transport. Alzheimer’s disease (AD) is one of the symptoms of adults with DS. The apoE allele e4 has been identified as a risk factor for AD and also, played a main role in nondisjunction. An increased risk of AD in mothers of adults with DS has been reported. We hypothesized that young mothers of DS children (Methods: In this case-control study, 33 DS children and their parents were compared in case of age with 90 families without any history of DS. Genotyping was performed by ARMS-PCR technique. Statistical analysis was performed by SPSS v.21 software. Results: It indicated that there is a significant difference in allele distribution between case and control groups. The C allele for 112 codon of APOE gene and the C allele for 158 codon of APOE gene may associate with nondisjunction. In 112 codon of APOE gene, it seems having T allele reduces the risk of nondisjunction and in contrast C allele may be a risk factor in happening of nondisjunction. (p-value = 0.000006, OR = 2.66, 95% CI = 1.74 - 4.06). In 158 codon of APOE gene, it seems having T allele reduces the risk of nondisjunction and in contrast C allele may be a risk factor in happening of nondisjunction. (p-value = 0.0000, OR = 3.89, 95% CI = 2.38 - 6.34). E4 allele frequency in mothers of DS is about 14% more than those in control group. According to results of this study the C allele in 158 codon of APOE gene and the C allele in 112 codon of APOE gene could be considered as susceptibility genetic factors for nondisjunction in Northeast of Iran.

Current Approach to Treatment of Juvenile Idiopathic Arthritis: Case Report of Hiperimmunglobulin E Syndrome Developed Juvenile Idiopathic Arthritis

Introduction: Juvenile idiopathic arthritis (JIA) represents a heterogeneous group of childhood chronic arthritic conditions. The pathogenesis of JIA remains incompletely understood. This disease can lead to a significant morbidity including joint deformity, growth impairment and a persistence of active arthritis into adulthood. The past two decades have witnessed significant advances in treatment and improved outcomes for affected children. With the current use of biologics, more target-specific, better tolerated, safer and more effective treatments have become possible. However, continuing, comprehensive follow-up is needed to characterize the long-term effects of such treatments. Hyperimmunoglobulin E syndrome (hyper-IgE, or Job’s syndrome) is a rare immune deficiency characterized by high IgE levels, atopic chronic eczema, tendency towards re-current pyogenic infection, neutrophil chemotaxis disorder and varying T-cell function impairment. Case Report: The case of a 17-year-old male patient with hyper-IgE who develops the oligoarticular subtype of JIA over a period of four years is discussed. The course of JIA is unfavorable, causing severe deformity of numerous joints (left elbow, right 3rd metacarpophalangeal, left knee, right ankle) and a fungal infection scar on the left eye. Blood tests show an ESR of 89 mm/h, rheumatoid factor (RF) 8.3 IU/mL (0 - 20) and positive antinuclear antibody (ANA). To improve gait, corrective surgery is performed on the right ankle, followed by rehabilitation and physical therapy. Conclusion: Developments in the near future will be crucial for understanding JIA pathophysiology and improving treatment.

血清微小RNA-199a-3p、E盒结合锌指蛋白1水平对急性冠状动脉综合征患者经皮冠状动脉介入术后预后的预测价值

目的探讨微小RNA-199a-3p(miR-199a-3p)、E盒结合锌指蛋白1(ZEB1)对急性冠状动脉综合征(ACS)患者经皮冠状动脉介入(PCI)术后预后的预测价值。方法选取2021年5月至2022年5月秦皇岛市第二医院接收的行PCI术治疗的113例ACS患者为观察组,根据观察组患者PCI术后1年内是否发生主要不良心血管事件(MACE),分为MACE组26例和非MACE组87例,同期健康体检者106例为对照组。检测血清miR-199a-3p、ZEB1水平,比较MACE组和非MACE组临床资料,Pearson分析患者血清miR-199a-3p和ZEB1水平相关性,多因素Logistic分析影响ACS患者PCI术后发生MACE的影响因素,ROC曲线分析血清miR-199a-3p、ZEB1对ACS患者PCI术后发生MACE的预测价值。结果与对照组相比,观察组血清miR-199a-3p水平较低,ZEB1水平较高(t=13.709、25.641,P<0.05);在ACS患者中miR-199a-3p与ZEB1呈负相关(r=-0.421,P<0.05);ACS患者PCI术后较PCI术前血清miR-199a-3p水平高,ZEB1水平低(t=4.820、6.040,P<0.05);MACE组与非MACE组高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、左心室射血分数、miR-199a-3p、ZEB1比较,差异有统计学意义(t=3.310、2.717、3.947、7.068、6.544,P<0.05);LDL-C、ZEB1水平是ACS患者PCI术后MACE的危险因素,左心室射血分数、HDL-C、miR-199a-3p水平是ACS患者PCI术后MACE的保护因素;miR-199a-3p、ZEB1联合预测ACS患者PCI术后MACE的曲线下面积为0.947,优于单独预测(Z=1.970、2.791,P<0.05)。结论ACS患者血清中miR-199a-3p、ZEB1水平呈负相关,两者均对ACS患者PCI术后MACE有预测价值,两者联合的预测价值更高。

参术定喘汤联合布地奈德福莫特罗对支气管哮喘慢性持续期患者中医证候、肺功能、FeNO、TIgE的影响及疗效观察

目的分析参术定喘汤联合布地奈德福莫特罗对支气管哮喘慢性持续期患者中医证候、肺功能、呼出气一氧化氮(fractional exhaled nitric oxide,FeNO)、血清总免疫球蛋白E(total immunoglobulin E,TIgE)的影响及疗效观察。方法选取2021年8月至2022年5月温州市中医院收治的支气管哮喘慢性持续期患者60例,采用随机数字表法分为对照组(n=30)和试验组(n=30)。对照组给予布地奈德福莫特罗,试验组给予参术定喘汤联合布地奈德福莫特罗治疗。评估两组中医证候评分水平,采用肺功能测定仪检测第一秒用力呼气容积(forced expiratory volume in one second,FEV1)、用力肺活量(forced vital capacity,FVC)、一秒率(forced expiratory volume in one second/forced vital capacity,FEV1/FVC),采用双抗体夹心酶联免疫吸附法检测TIgE水平,呼气分析仪检测FeNO水平。结果治疗后,试验组中医证候评分水平、Fe NO、TIgE均低于对照组,差异有统计学意义(P<0.05);FEV1、FVC、FEV1/FVC水平均高于对照组,差异有统计学意义(P<0.05)。试验组总有效率高于对照组,差异有统计学意义(P<0.05)。结论对支气管哮喘慢性持续期患者采用参术定喘汤进行治疗,能够显著降低中医证候水平、改善患者肺功能、下调Fe NO、TIgE水平,临床治疗效果显著。

血清IgE和NGAL在小儿肾病综合征激素耐药评估中的应用价值

目的研究血清免疫球蛋白E(IgE)和中性粒细胞明胶酶相关脂质运载蛋白(NGAL)在小儿肾病综合征(NS)激素耐药评估中的应用价值。方法选取西安市儿童医院2017年4月至2021年3月收治的180例NS患儿作为研究对象,根据患儿治疗4周后尿蛋白是否转阴将其分为耐药组(35例)和敏感组(145例)。纳入同期入院体检的60例健康小儿作为对照组。比较三组的血清IgE和NGAL水平;使用受试者工作特征(ROC)曲线分析IgE和NGAL对NS患儿激素耐药的预测价值;使用二元Logistic回归分析IgE、NGAL阳性与NS患儿激素耐药的相关性。结果对照组的IgE和NGAL水平均明显低于耐药组和敏感组,差异具有统计学意义(P<0.05)。耐药组的IgE水平明显低于敏感组,NGAL水平明显高于敏感组,差异具有统计学意义(P<0.05)。IgE预测NS患儿激素耐药的曲线下面积(AUC)为0.879(95%CI 0.816~0.941),灵敏度为80.00%,特异度为85.52%;NGAL预测NS患儿激素耐药的AUC为0.854(95%CI 0.795~0.914),灵敏度为82.86%,特异度为77.24%。IgE阳性和NGAL阳性均为NS患儿激素耐药发生的独立危险因素(P<0.05)。结论血清IgE和NGAL对NS患儿激素耐药的预测价值良好,且均为NS患儿激素耐药发生的独立危险因素。

脐血维生素A和维生素E水平与早产儿发生呼吸窘迫综合征的关系研究

背景呼吸窘迫综合征(RDS)是导致早产儿死亡的重要原因,需不断加强临床预防及治疗,但目前关于脐血维生素A、E水平与早产儿发生RDS的关系研究报道较少。目的探讨脐血维生素A、E水平与早产儿发生RDS的关系。方法选取2021年1月—2022年1月在石家庄市第四医院出生的早产儿304例,根据RDS发生情况将其分为RDS组120例与非RDS组184例。收集与早产儿发生RDS可能相关的临床特征并检测脐血维生素A、E水平;早产儿发生RDS及RDS严重程度的影响因素分析采用多因素Logistic回归分析。结果RDS组早产儿胎龄、出生体质量小于非RDS组,1 min Apgar评分≤7分比例、5 min Apgar评分≤7分比例、维生素A缺乏发生率高于非RDS组,脐血维生素A、E水平低于非RDS组(P<0.05)。RDS组早产儿中轻症86例,重症34例;重症RDS早产儿脐血维生素A水平低于轻症RDS早产儿,维生素A缺乏发生率高于轻症RDS早产儿(P<0.05)。多因素Logistic回归分析结果显示,脐血维生素A水平为早产儿发生RDS的影响因素[OR=2.208,95%CI(1.156,4.218),P<0.05];维生素A缺乏为早产儿发生重症RDS的影响因素[OR=6.835,95%CI(2.537,18.416),P<0.05]。结论发生RDS的早产儿脐血维生素A、E水平较低,脐血维生素A水平为早产儿发生RDS及其严重程度的影响因素,建议在妊娠期适当补充维生素A以减少早产儿RDS的发生及减轻其严重程度。

过敏性鼻炎中医辨证分型及其与IgE、cd4、cd45的相关性研究

目的分析过敏性鼻炎中医辨证分型及其与特异性免疫球蛋白E(Immunog lobulin E,IgE)、cd4、cd45的相关性。方法选取2019年12月-2022年5月我院门诊收治的50例过敏性鼻炎患者为研究组,另选取同期于我院进行健康体检的志愿者48例为对照组。对研究组过敏性鼻炎患者进行中医辨证分型,检测2组IgE、cd4、cd45水平,分析中医辨证分型与IgE、cd4、cd45的相关性。结果与对照组相比,研究组IgE、cd45水平较高,cd4水平较低(P<0.05);肺经伏热型、肾阳不足型、脾气虚弱型患者IgE、cd4、cd45水平相比,差异无统计学意义(P>0.05);与肺经伏热型、肾阳不足型、脾气虚弱型相比,肺气虚寒型过敏性鼻炎患者IgE、cd45水平较高,cd4水平较低(P<0.05);IgE、cd4、cd45与肺经伏热型、肾阳不足型、脾气虚弱型、肺气虚寒型存在联系。结论过敏性鼻炎患者IgE、cd45呈高表示,cd4呈低表达,且在肺气虚寒型过敏性鼻炎患者IgE、cd45水平最高,cd4水平最低,检测IgE、cd4、cd45可为过敏性鼻炎患者中医辨证分型、临床治疗提供借鉴。

维生素E联合克罗米芬治疗多囊卵巢综合征不孕症的效果

目的:探讨维生素E联合克罗米芬联合治疗多囊卵巢综合征(PCOS)不孕症的临床效果。方法:选择2018年5月—2019年5月在广州市妇女儿童医疗中心就诊的86例PCOS不孕症患者,采用随机数表法将其分为两组,各43例。对照组患者口服克罗米芬,观察组患者在对照组基础上联合使用维生素E治疗。评估临床疗效,记录雌激素水平及成熟卵泡数,对用药前和用药后的氧化应激指标进行对比观察,并接受B超检查,观察子宫内膜和卵泡发育情况。结果:观察组排卵率、妊娠率为95.35%、46.51%,与对照组81.40%、25.58%相比明显更高(P<0.05);观察组未破裂卵泡黄素化综合征(LUFS)发生率为6.98%,与对照组23.26%相比明显更低(P<0.05),两组卵巢过度刺激综合征(OHSS)发生率比较差异无统计学意义(P>0.05)。观察组治疗后雌二醇(E_(2))指标高于对照组,卵泡大小及成熟卵泡数均优于对照组,且观察组子宫内膜厚度较对照组厚(P<0.05)。两组用药后血清超氧化物歧化酶(SOD)指标上升,且观察组SOD明显高于对照组(P<0.05)。结论:临床针对PCOS不孕症患者,用维生素与克罗米芬联合治疗效果突出,能提高雌激素水平及氧化应激指标。

High-intensity swimming alleviates nociception and neuroinflammation in a mouse model of chronic postischemia pain by activating the resolvin E1-chemerin receptor 23 axis in the spinal cord

Physical exe rcise effectively alleviates chronic pain associated with complex regional pain syndrome type-Ⅰ.However,the mechanism of exe rcise-induced analgesia has not been clarified.Recent studies have shown that the specialized pro-resolving lipid mediator resolvin E1 promotes relief of pathologic pain by binding to chemerin receptor 23 in the nervous system.However,whether the resolvin E1-chemerin receptor 23 axis is involved in exercise-induced analgesia in complex regional pain syndrome type-Ⅰ has not been demonstrated.In the present study,a mouse model of chronic post-ischemia pain was established to mimic complex regional pain syndrome type-Ⅰ and subjected to an intervention involving swimming at different intensities.Chronic pain was reduced only in mice that engaged in high-intensity swimming.The resolvin E1-chemerin receptor 23 axis was clearly downregulated in the spinal cord of mice with chronic pain,while high-intensity swimming restored expression of resolvin E1 and chemerin receptor 23.Finally,shRNA-mediated silencing of chemerin receptor 23in the spinal cord reve rsed the analgesic effect of high-intensity swimming exercise on chronic post-ischemic pain and the anti-inflammato ry pola rization of microglia in the dorsal horn of the spinal cord.These findings suggest that high-intensity swimming can decrease chronic pain via the endogenous resolvin E1-chemerin receptor 23 axis in the spinal cord.

耳穴埋针联合药物治疗小儿过敏性鼻炎-哮喘综合征的疗效观察及对肺功能和血清IgE、EOS、FeNO的影响

目的观察耳穴埋针联合药物治疗小儿过敏性鼻炎-哮喘综合征(combined allergic rhinitis and asthma syndrome,CARAS)的临床疗效及对肺功能和血清免疫球蛋白E(immunoglobulin E,IgE)、嗜酸性粒细胞(eosinophilic,EOS)、呼出气一氧化氮(fractional exhaled nitric oxide,FeNO)的影响。方法将84例CARAS患者随机分为观察组和对照组,每组42例。对照组予布地奈德雾化吸入,孟鲁司特钠睡前口服治疗;在此基础上观察组予耳穴埋针治疗。观察两组主要症状(鼻塞、鼻痒、喷嚏、清涕、气喘、胸闷、咳嗽、喉间痰鸣)积分、肺功能指标[(1秒用力呼气容积(forced expiratory volume in one second,FEV1)、用力肺活量(forced vital capacity,FVC)、FEV1/FVC、呼气峰流速(peak expiratory flow,PEF)]及血清Ig E、EOS和FeNO水平变化,并比较两组临床疗效。结果观察组总有效率为90.5%,与对照组的71.4%比较,差异有统计学意义(P<0.01)。治疗后,观察组主要症状积分均较治疗前改善,且治疗组优于对照组,差异有统计学意义(P<0.01)。治疗后,两组肺功能指标、血清Ig E、EOS和Fe NO水平与治疗前比较,差异均有统计学意义(P<0.01);且治疗组均优于对照组,差异有统计学意义(P<0.05)。结论耳穴埋针联合药物治疗小儿CARAS疗效显著,不仅能够改善患者鼻炎、哮喘症状,且能有效促进肺功能恢复,改善血清IgE、EOS和FeNO水平。

Vitamin E modulates androgen receptor gene expression to attenuate ovarian dysfunctions in a rat model of dehydroepiandrosterone-induced polycystic ovary

Objective:To investigate the protective effect of vitamin E in dehydroepiandrosterone(DHEA)-induced polycystic ovary in rats.Methods:Premature female Wistar rats were randomly allocated into four groups,with 7 rats in each group.Group栺received corn oil(vehicle)and served as the control group;group栻received 0.2 mL of 0.06 mg/g DHEA in corn oil;group栿received 200 mg/kg vitamin E;group桇received DHEA plus vitamin E.All treatments lasted for 15 days,with DHEA administered subcutaneously,while vitamin E and corn oil were administered orally.After the experiment,serum samples and ovaries were harvested for biochemical,immunohistochemical,hormonal,and histological analysis.The ovarian mRNA expression of androgen receptor was analyzed by reverse transcriptase quantitative polymerase chain reaction(qPCR).Results:The antioxidant and metabolic enzyme activity significantly decreased in the DHEA-treated rats compared to the control rats(P<0.05).Administration of vitamin E to DHEAtreated rats significantly decreased cytokines and malondialdehyde compared to the DHEA-treated rats.The histological analysis showed reduced atretic and cystic ovaries,increased E-cadherin and Bcl-2 expression,and reduced expression of Bax in the DHEAtreated rats co-treated with vitamin E.The mRNA expression of androgen receptor was upregulated in the DHEA-treated rats compared to the control rats.Conclusions:Vitamin E ameliorates the hyperandrogenic effect of DHEA-induced polycystic ovaries via metabolic,antioxidant,and anti-apoptotic pathways.

淋巴细胞抗原6复合物基因座E抑制新型冠状病毒不同变异株刺突蛋白介导的感染侵入

目的研究淋巴细胞抗原6复合物,基因座E(lymphocyte antigen 6 complex,locus E,LY6E)对新型冠状病毒(severe acute respiratpry syndrome coronavirus 2,SARS-CoV-2)不同变异株刺突蛋白(spike protein,S蛋白)介导的感染侵入的抑制作用。方法构建来源于人的血管紧张素转换酶2(angiotensin-converting enzyme 2,ACE2)表达质粒;建立Flp-In T-Rex 293/LY6E诱导表达细胞系和Flp-In T-Rex 293/氯霉素乙酰转移酶(chloramphenicol acetyltransferase,CAT)诱导表达细胞系,转染人ACE2表达质粒,并通过免疫印迹方法检测ACE2和LY6E在T-Rex 293细胞中的表达情况;构建SARS-CoV-2(Wuhan-Hu-1株系、D614G突变株系、Delta突变株系,以及Omicron BA.1、BA.2、BA.2.12.1、BA.3、BA.4/5、BF.7突变株系)和拉沙热病毒(Lassa fever virus,LASV)假病毒感染系统,利用假病毒荧光素酶报告基因检测LY6E对SARS-CoV-2及其突变株刺突蛋白介导侵入的抑制作用;通过Nano-Glo活细胞检测系统,检测LY6E对SARS-CoV-2突变株刺突蛋白介导的合胞体形成的抑制作用;构建甲型流感病毒(influenza A virus,IAV)假病毒感染系统,经两性霉素B处理后,利用假病毒荧光素酶报告基因检测LY6E对SARS-CoV-2及其突变株刺突蛋白介导侵入的抑制作用。结果构建的Flp-In T-Rex293/LY6E诱导表达细胞系对SARS-CoV-2(Wuhan-Hu-1株系、D614G突变株系、Delta突变株系,以及Omicron BA.1突变株系)假病毒侵入细胞有明显抑制作用,SARS-CoV-2假病毒感染四环素(tetracycline,Tet)处理组与非处理组的相对感染率差异有统计学意义[SARS-CoV-2野生型株系假病毒(WTpp):t=33.920,P<0.001;SARS-CoV-2突变株系假病毒(D614Gpp):t=31.478,P<0.001;Deltapp:t=30.257,P<0.001;Omicronpp:t=21.041,P<0.001];LY6E对SARS-CoV-2不同突变株S蛋白介导的合胞体形成有明显抑制作用,LY6E表达组与未表达组的相对荧光单位差异有统计学意义(D614G:t=18.90,P<0.001;Delta:t=22.28,P<0.001;BA.1:t=8.995,P<0.001;BA.2:t=13.57,P<0.001;BA.2.12.1:t=15.48,P<0.001;BA.3:t=13.65,P<0.001;BA.4/5:t=16.74,P<0.001;BF.7:t=22.29,P<0.001);两性霉素B处理没有改变Flp-In T-Rex 293/LY6E诱导表达细胞系对SARS-CoV-2(Wuhan-Hu-1株系、D614G突变株系、Delta突变株系,以及Omicron BA.1突变株系)假病毒侵入细胞的抑制作用,也没有改变LY6E对IAV感染的增强作用,IAV假病毒感染Tet处理组与非处理组的相对感染率差异有统计学意义(t=3.343,P<0.05)。结论LY6E抑制SARS-CoV-2野生型(Wuhan-Hu-1株系)及多种突变株(D614G突变株系、Delta突变株系,以及Omicron突变株系)刺突蛋白介导的感染侵入;两性霉素B没有改变LY6E对SARS-CoV-2(Wuhan-Hu-1株系、D614G突变株系、Delta突变株系,以及Omicron突变株系)的抑制作用。