The changes of blood perfusion and oxygen transport in tumors during tumor vascular normalization are studied with 3-dimensional mathematical modeling and numerical simulation. The models of tumor angiogenesis and vas...The changes of blood perfusion and oxygen transport in tumors during tumor vascular normalization are studied with 3-dimensional mathematical modeling and numerical simulation. The models of tumor angiogenesis and vascular-disrupting are used to simulate ＂un-normalized＂ and ＂normalized＂ vasculatures. A new model combining tumor hemodynamics and oxygen transport is developed. In this model, the intravasculartransvascular-interstitial flow with red blood cell（RBC） delivery is tightly coupled, and the oxygen resource is produced by heterogeneous distribution of hematocrit from the flow simulation. The results show that both tumor blood perfusion and hematocrit in the vessels increase, and the hypoxia microenvironment in the tumor center is greatly improved during vascular normalization. The total oxygen content inside the tumor tissue increases by about 67%, 51%, and 95% for the three approaches of vascular normalization,respectively. The elevation of oxygen concentration in tumors can improve its metabolic environment, and consequently reduce malignancy of tumor cells. It can also enhance radiation and chemotherapeutics to tumors.展开更多
目的通过比较西藏自治区阿里地区人民医院与陕西省两所医院健康新生儿出生后血氧饱和度(SpO_(2))水平和血常规水平,探索西藏自治区阿里地区新生儿出生后SpO_(2)水平与红细胞形态、功能的特点。方法本研究为盲法回顾性研究。选取阿里地...目的通过比较西藏自治区阿里地区人民医院与陕西省两所医院健康新生儿出生后血氧饱和度(SpO_(2))水平和血常规水平,探索西藏自治区阿里地区新生儿出生后SpO_(2)水平与红细胞形态、功能的特点。方法本研究为盲法回顾性研究。选取阿里地区人民医院、泾阳县人民医院和耀州区人民医院新生儿科2020年1月至2020年7月正常出生的167例足月新生儿,阿里地区人民医院出生新生儿(74例)为高原组,陕西省两所医院出生新生儿(93例)为平原组。监测正常足月新生儿断脐后10 min右上肢SpO_(2)及心率水平,同时采集废弃脐血进行血细胞分析。比较高原组与平原组足月正常新生儿出生后10 min SpO_(2)水平特点,与《新生儿复苏指南(2021年)》(后文统一简称《指南》)中SpO_(2)目标值进行比较,同时比较两组血细胞分析结果的差异。结果高原组出生新生儿出生1~10 min SpO_(2)水平低于平原组,差异有统计学意义(P<0.05)。高原组出生新生儿脐血白细胞计数、血小板计数低于平原组,差异有统计学意义(P<0.05);红细胞计数、血红蛋白高于平原组,差异有统计学意义(P<0.05);红细胞平均体积小于平原组,差异有统计学意义(P<0.05),平均血红蛋白量、平均血红蛋白浓度、红细胞分布宽度标准偏差及红细胞分布宽度变异系数高于平原组,差异有统计学意义(P<0.05)。高原组出生新生儿1~8 min SpO_(2)水平与《指南》目标值符合率明显低于平原组,差异有统计学意义(P<0.05)。高原组出生新生儿出生后第9 min、出生后第10 min,SpO_(2)水平与《指南》目标值符合率为100%,与平原组比较差异无统计学意义(P>0.05)。结论《指南》中出生后10 min SpO_(2)预期值适用于西藏自治区阿里地区新生儿,脐血红细胞特有形态及高血红蛋白含量和浓度可能是常驻藏族新生儿适应高原的原因之一。展开更多
Background:Hyperbaric oxygen treatment(HBOT)has been demonstrated to influence the keloid recurrence rate after surgery and to relieve keloid symptoms and other pathological processes in keloids.To explore the mechani...Background:Hyperbaric oxygen treatment(HBOT)has been demonstrated to influence the keloid recurrence rate after surgery and to relieve keloid symptoms and other pathological processes in keloids.To explore the mechanism of the effect of HBOT on keloids,tumor immune gene expression and immune cell infiltration were studied in this work.Methods:From February 2021 to April 2021,HBOT was carried out on keloid patients four times before surgery.Keloid tissue samples were collected and divided into an HBOT group(keloid with HBOT before surgery[HK]group,n=6)and a non-HBOT group(K group,n=6).Tumor gene expression was analyzed with an Oncomine Immune Response Research Assay kit.Data were mined with R package.The differentially expressed genes between the groups were compared.Hub genes between the groups were determined and verified with Quantitative Real-time PCR.Immune cell infiltration was analyzed based on CIBERSORT deconvolution algorithm analysis of gene expression and verified with immunohistochemistry(IHC).Results:Inflammatory cell infiltration was reduced in the HK group.There were 178 upregulated genes and 217 downregulated genes.Ten hub genes were identified,including Integrin Subunit Alpha M(ITGAM),interleukin(IL)-4,IL-6,IL-2,Protein Tyrosine Phosphatase Receptor Type C(PTPRC),CD86,transforming growth factor(TGF),CD80,CTLA4,and IL-10.CD80,ITGAM,IL-4,and PTPRC with significantly downregulated expression were identified.IL-10 and IL-2 were upregulated in the HK group but without a significant difference.Infiltration differences of CD8 lymphocyte T cells,CD4 lymphocyte T-activated memory cells,and dendritic resting cells were identified with gene CIBERSORT deconvolution algorithm analysis.Infiltration levels of CD4 lymphocyte T cell in the HK group were significantly higher than those of the K group in IHC verification.Conclusion:HBOT affected tumor gene expression and immune cell infiltration in keloids.CD4 lymphocyte T cell,especially activated memory CD4+T,might be the key regulatory immune cell,and its related gene expression needs further study.展开更多
目的:观察炙甘草汤联合针刺治疗老年肿瘤相关性贫血的临床疗效。方法:选择2018年12月至2021年12月在上海德济医院治疗的老年肿瘤相关性贫血患者60例,按照随机数字表法分为对照组和观察组,每组各30例。对照组给予常规西医支持治疗,观察...目的:观察炙甘草汤联合针刺治疗老年肿瘤相关性贫血的临床疗效。方法:选择2018年12月至2021年12月在上海德济医院治疗的老年肿瘤相关性贫血患者60例,按照随机数字表法分为对照组和观察组,每组各30例。对照组给予常规西医支持治疗,观察组在对照组治疗的基础上给予炙甘草汤联合针刺治疗。观察两组治疗前后血红蛋白、Karnofsky功能状态评分(Karnofsky performance status score, KPS)、外周血T细胞亚群(CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+))变化情况及临床疗效、不良反应发生情况。结果:观察组有效率为100.00%,对照组有效率为93.33%,观察组有效率高于对照组,差异有统计学意义(P<0.05);观察组治疗后血红蛋白、KPS评分均高于对照组,差异有统计学意义(P<0.05);观察组治疗后CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)均高于对照组,差异有统计学意义(P<0.05);观察组不良反应发生率为10.00%,对照组不良反应发生率为6.67%,两组比较差异无统计学意义(P>0.05)。结论:炙甘草汤联合针刺治疗老年肿瘤相关性贫血,可以提高临床疗效,改善患者生活质量、血红蛋白水平、外周血T细胞亚群水平。展开更多
基金Project supported by the National Natural Science Foundation of China(Nos.11102113 and81301816)the New Teachers Start Program of Shanghai Jiao Tong University+1 种基金the Chenxing Young Scholars Program B of Shanghai Jiao Tong University(No.13X100010070)the Natural Science Research Foundation of Shanghai Jiao Tong University School of Medicine(No.13XJ10037)
文摘The changes of blood perfusion and oxygen transport in tumors during tumor vascular normalization are studied with 3-dimensional mathematical modeling and numerical simulation. The models of tumor angiogenesis and vascular-disrupting are used to simulate ＂un-normalized＂ and ＂normalized＂ vasculatures. A new model combining tumor hemodynamics and oxygen transport is developed. In this model, the intravasculartransvascular-interstitial flow with red blood cell（RBC） delivery is tightly coupled, and the oxygen resource is produced by heterogeneous distribution of hematocrit from the flow simulation. The results show that both tumor blood perfusion and hematocrit in the vessels increase, and the hypoxia microenvironment in the tumor center is greatly improved during vascular normalization. The total oxygen content inside the tumor tissue increases by about 67%, 51%, and 95% for the three approaches of vascular normalization,respectively. The elevation of oxygen concentration in tumors can improve its metabolic environment, and consequently reduce malignancy of tumor cells. It can also enhance radiation and chemotherapeutics to tumors.
文摘目的通过比较西藏自治区阿里地区人民医院与陕西省两所医院健康新生儿出生后血氧饱和度(SpO_(2))水平和血常规水平,探索西藏自治区阿里地区新生儿出生后SpO_(2)水平与红细胞形态、功能的特点。方法本研究为盲法回顾性研究。选取阿里地区人民医院、泾阳县人民医院和耀州区人民医院新生儿科2020年1月至2020年7月正常出生的167例足月新生儿,阿里地区人民医院出生新生儿(74例)为高原组,陕西省两所医院出生新生儿(93例)为平原组。监测正常足月新生儿断脐后10 min右上肢SpO_(2)及心率水平,同时采集废弃脐血进行血细胞分析。比较高原组与平原组足月正常新生儿出生后10 min SpO_(2)水平特点,与《新生儿复苏指南(2021年)》(后文统一简称《指南》)中SpO_(2)目标值进行比较,同时比较两组血细胞分析结果的差异。结果高原组出生新生儿出生1~10 min SpO_(2)水平低于平原组,差异有统计学意义(P<0.05)。高原组出生新生儿脐血白细胞计数、血小板计数低于平原组,差异有统计学意义(P<0.05);红细胞计数、血红蛋白高于平原组,差异有统计学意义(P<0.05);红细胞平均体积小于平原组,差异有统计学意义(P<0.05),平均血红蛋白量、平均血红蛋白浓度、红细胞分布宽度标准偏差及红细胞分布宽度变异系数高于平原组,差异有统计学意义(P<0.05)。高原组出生新生儿1~8 min SpO_(2)水平与《指南》目标值符合率明显低于平原组,差异有统计学意义(P<0.05)。高原组出生新生儿出生后第9 min、出生后第10 min,SpO_(2)水平与《指南》目标值符合率为100%,与平原组比较差异无统计学意义(P>0.05)。结论《指南》中出生后10 min SpO_(2)预期值适用于西藏自治区阿里地区新生儿,脐血红细胞特有形态及高血红蛋白含量和浓度可能是常驻藏族新生儿适应高原的原因之一。
基金supported by a grant from the National Natural Science Foundation of China(No.81871538).
文摘Background:Hyperbaric oxygen treatment(HBOT)has been demonstrated to influence the keloid recurrence rate after surgery and to relieve keloid symptoms and other pathological processes in keloids.To explore the mechanism of the effect of HBOT on keloids,tumor immune gene expression and immune cell infiltration were studied in this work.Methods:From February 2021 to April 2021,HBOT was carried out on keloid patients four times before surgery.Keloid tissue samples were collected and divided into an HBOT group(keloid with HBOT before surgery[HK]group,n=6)and a non-HBOT group(K group,n=6).Tumor gene expression was analyzed with an Oncomine Immune Response Research Assay kit.Data were mined with R package.The differentially expressed genes between the groups were compared.Hub genes between the groups were determined and verified with Quantitative Real-time PCR.Immune cell infiltration was analyzed based on CIBERSORT deconvolution algorithm analysis of gene expression and verified with immunohistochemistry(IHC).Results:Inflammatory cell infiltration was reduced in the HK group.There were 178 upregulated genes and 217 downregulated genes.Ten hub genes were identified,including Integrin Subunit Alpha M(ITGAM),interleukin(IL)-4,IL-6,IL-2,Protein Tyrosine Phosphatase Receptor Type C(PTPRC),CD86,transforming growth factor(TGF),CD80,CTLA4,and IL-10.CD80,ITGAM,IL-4,and PTPRC with significantly downregulated expression were identified.IL-10 and IL-2 were upregulated in the HK group but without a significant difference.Infiltration differences of CD8 lymphocyte T cells,CD4 lymphocyte T-activated memory cells,and dendritic resting cells were identified with gene CIBERSORT deconvolution algorithm analysis.Infiltration levels of CD4 lymphocyte T cell in the HK group were significantly higher than those of the K group in IHC verification.Conclusion:HBOT affected tumor gene expression and immune cell infiltration in keloids.CD4 lymphocyte T cell,especially activated memory CD4+T,might be the key regulatory immune cell,and its related gene expression needs further study.
文摘目的:观察炙甘草汤联合针刺治疗老年肿瘤相关性贫血的临床疗效。方法:选择2018年12月至2021年12月在上海德济医院治疗的老年肿瘤相关性贫血患者60例,按照随机数字表法分为对照组和观察组,每组各30例。对照组给予常规西医支持治疗,观察组在对照组治疗的基础上给予炙甘草汤联合针刺治疗。观察两组治疗前后血红蛋白、Karnofsky功能状态评分(Karnofsky performance status score, KPS)、外周血T细胞亚群(CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+))变化情况及临床疗效、不良反应发生情况。结果:观察组有效率为100.00%,对照组有效率为93.33%,观察组有效率高于对照组,差异有统计学意义(P<0.05);观察组治疗后血红蛋白、KPS评分均高于对照组,差异有统计学意义(P<0.05);观察组治疗后CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)均高于对照组,差异有统计学意义(P<0.05);观察组不良反应发生率为10.00%,对照组不良反应发生率为6.67%,两组比较差异无统计学意义(P>0.05)。结论:炙甘草汤联合针刺治疗老年肿瘤相关性贫血,可以提高临床疗效,改善患者生活质量、血红蛋白水平、外周血T细胞亚群水平。
文摘目的探讨基于核转录因子-κB(nuclear transcription factor-κB,NF-κB)信号通路的高压氧干预对骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)移植治疗脊髓损伤大鼠炎症因子及血糖水平的影响.方法SD大鼠40只随机分为假手术组、模型组、BMSCs组、BMSCs+高压氧组,每组10只,采用改良Allen打击法制备大鼠脊髓损伤模型,BMSCs组于造模1 h后尾静脉注射1 mL BMSCs悬液(3×10^6个);BMSCs+高压氧组于造模1 h后尾静脉注射1 mL BMSCs悬液(3×10^6个)+高压氧.术后第8天比较各组大鼠Basso-Beattie-Bresnahan(BBB)评分,比较各组大鼠脊髓组织病理学变化,检测并比较各组大鼠血清中白细胞介素1β(interleukin-1β,IL-1β)、肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、血糖含量,检测并比较各组大鼠脊髓组织中NF-κB、核因子κB抑制蛋白α(inhibitorg protein of NF-κB-α,IκB-α)蛋白水平.结果各组大鼠BBB评分差异比较具有统计学意义(P<0.05);各组大鼠血清中TNF-α、IL-1β、血糖含量差异比较具有统计学意义(P<0.05),各组大鼠脊髓组织中NF-κB、IκB-α蛋白水平差异比较具有统计学意义(P<0.05).BMSCs+高压氧组较模型组、BMSCs组脊髓组织肿胀明显减轻,炎性细胞浸润明显减少,损伤区域结构改善更加明显.结论基于NF-κB信号通路的高压氧干预能够抑制BMSCs移植治疗脊髓损伤大鼠炎症因子表达,降低血糖水平,减轻脊髓损伤后病理变化,促进脊髓损伤修复.
