Hyperoside is a bioactive flavonoid galactoside in both medicinal and edible plants.It plays an important physiological role in the growth of flower buds.However,the hyperoside biosynthesis pathway has not been system...Hyperoside is a bioactive flavonoid galactoside in both medicinal and edible plants.It plays an important physiological role in the growth of flower buds.However,the hyperoside biosynthesis pathway has not been systematically elucidated in plants,including its original source,Hypericaceae.Our group found abundant hyperoside in the flower buds of Hypericum monogynum,and we sequenced its transcriptome to study the biosynthetic mechanism of hyperoside.After gene screening and functional verification,four kinds of key enzymes were identified.Specifically,HmF3Hs(flavanone 3-hydroxylases)and HmFLSs(flavonol synthases)could catalyze flavanones into dihydroflavonols,as well as catalyzing dihydroflavonols into flavonols.HmFLSs could also convert flavanones into flavonols and flavones with varying efficiencies.HmF3′H(flavonoid 3′-hydroxylase)was found to act broadly on 4′-hydroxyl flavonoids to produce 3′,4′-diydroxylated flavanones,dihydroflavonols,flavonols,and flavones.HmGAT(flavonoid 3-O-galactosyltransferase)would transform flavonols into the corresponding 3-O-galactosides,including hyperoside.The parallel hyperoside biosynthesis routes were thus depicted,one of which was successfully reconstructed in Escherichia coli BL21(DE3)by feeding naringenin,resulting in a hyperoside yield of 25 mg/l.Overall,this research not only helped us understand the interior catalytic mechanism of hyperoside in H.monogynum concerning flower development and bioactivity,but also provided valuable insights into these enzyme families.展开更多
When discovering the potential of canards flying in 4-dimensional slow-fast system with a bifurcation parameter, the key notion “symmetry” plays an important role. It is of one parameter on slow vector field. Then, ...When discovering the potential of canards flying in 4-dimensional slow-fast system with a bifurcation parameter, the key notion “symmetry” plays an important role. It is of one parameter on slow vector field. Then, it should be determined to introduce parameters to all slow/fast vectors. It is, however, there might be no way to explore for another potential in this system, because the geometrical structure is quite different from the system with one parameter. Even in this system, the “symmetry” is also useful to obtain the potentials classified by R. Thom. In this paper, via the coordinates changing, the possible way to explore for the potential will be shown. As it is analyzed on “hyper finite time line”, or done by using “non-standard analysis”, it is called “Hyper Catastrophe”. In the slow-fast system which includes a very small parameter , it is difficult to do precise analysis. Thus, it is useful to get the orbits as a singular limit. When trying to do simulations, it is also faced with difficulty due to singularity. Using very small time intervals corresponding small , we shall overcome the difficulty, because the difference equation on the small time interval adopts the standard differential equation. These small intervals are defined on hyper finite number N, which is nonstandard. As and the intervals are linked to use 1/N, the simulation should be done exactly.展开更多
Surface acoustic wave (SAW) technology has been extensively explored for wireless communication, sensors, microfluidics, photonics, and quantum information processing. However, due to fabrication issues, the frequenci...Surface acoustic wave (SAW) technology has been extensively explored for wireless communication, sensors, microfluidics, photonics, and quantum information processing. However, due to fabrication issues, the frequencies of SAW devices are typically limited to within a few gigahertz, which severely restricts their applications in 5G communication, precision sensing, photonics, and quantum control. To solve this critical problem, we propose a hybrid strategy that integrates a nanomanufacturing process (i.e., nanolithography) with a LiNbO_(3)/SiO_(2)/SiC heterostructure and successfully achieve a record-breaking frequency of about 44 GHz for SAW devices, in addition to large electromechanical coupling coefficients of up to 15.7%. We perform a theoretical analysis and identify the guided higher order wave modes generated on these slow-on-fast SAW platforms. To demonstrate the superior sensing performance of the proposed ultra-high-frequency SAW platforms, we perform micro-mass sensing and obtain an extremely high sensitivity of approximately 33151.9 MHz·mm2·μg−1, which is about 1011 times higher than that of a conventional quartz crystal microbalance (QCM) and about 4000 times higher than that of a conventional SAW device with a frequency of 978 MHz.展开更多
Due to its complexity and involvement of numerous stakeholders,the pharmaceutical supply chain presents many challenges that companies must overcome to deliver necessary medications to patients efficiently.The pharmac...Due to its complexity and involvement of numerous stakeholders,the pharmaceutical supply chain presents many challenges that companies must overcome to deliver necessary medications to patients efficiently.The pharmaceutical supply chain poses different challenging issues,encompasses supply chain visibility,cold-chain shipping,drug counterfeiting,and rising prescription drug prices,which can considerably surge out-of-pocket patient costs.Blockchain(BC)offers the technical base for such a scheme,as it could track legitimate drugs and avoid fake circulation.The designers presented the procedure of BC with fabric for creating a secured drug supplychain management(DSCM)method.With this motivation,the study presents a new blockchain with optimal deep learning-enabled DSCM and recommendation scheme(BCODL-DSCMRS)for Pharmaceutical Industries.Firstly,Hyperledger fabric is used for DSC management,enabling effective tracking processes in the smart pharmaceutical industry.In addition,a hybrid deep belief network(HDBN)model is used to suggest the best or top-rated medicines to healthcare providers and consumers.The spotted hyena optimizer(SHO)algorithm is used to optimize the performance of the HDBN model.The design of the HSO algorithm for tuning the HDBN model demonstrates the novelty of the work.The presented model is tested on the UCI repository’s open-access drug reviews database.展开更多
In the Digital World scenario,the confidentiality of information in video transmission plays an important role.Chaotic systems have been shown to be effective for video signal encryption.To improve video transmission ...In the Digital World scenario,the confidentiality of information in video transmission plays an important role.Chaotic systems have been shown to be effective for video signal encryption.To improve video transmission secrecy,compressive encryption method is proposed to accomplish compression and encryption based on fractional order hyper chaotic system that incorporates Compressive Sensing(CS),pixel level,bit level scrambling and nucleotide Sequences operations.The measurement matrix generates by the fractional order hyper chaotic system strengthens the efficiency of the encryption process.To avoid plain text attack,the CS measurement is scrambled to its pixel level,bit level scrambling decreases the similarity between the adjacent measurements and the nucleotide sequence operations are done on the scrambled bits,increasing the encryption.Two stages are comprised in the reconstruction technique,the first stage uses the intra-frame similarity and offers robust preliminary retrieval for each frame,and the second stage iteratively improves the efficiency of reconstruction by integrating inter frame Multi Hypothesis(MH)estimation and weighted residual sparsity modeling.In each iteration,the residual coefficient weights are modified using a mathematical approach based on the MH predictions,and the Split Bregman iteration algorithm is defined to resolve weighted l1 regularization.Experimental findings show that the proposed algorithm provides good compression of video coupled with an efficient encryption method that is resistant to multiple attacks.展开更多
基金supported by the National Natural Science Foundation of China(No.32070389)the‘Double First-Class’University project of China Pharmaceutical University(CPU2022QZ29).
文摘Hyperoside is a bioactive flavonoid galactoside in both medicinal and edible plants.It plays an important physiological role in the growth of flower buds.However,the hyperoside biosynthesis pathway has not been systematically elucidated in plants,including its original source,Hypericaceae.Our group found abundant hyperoside in the flower buds of Hypericum monogynum,and we sequenced its transcriptome to study the biosynthetic mechanism of hyperoside.After gene screening and functional verification,four kinds of key enzymes were identified.Specifically,HmF3Hs(flavanone 3-hydroxylases)and HmFLSs(flavonol synthases)could catalyze flavanones into dihydroflavonols,as well as catalyzing dihydroflavonols into flavonols.HmFLSs could also convert flavanones into flavonols and flavones with varying efficiencies.HmF3′H(flavonoid 3′-hydroxylase)was found to act broadly on 4′-hydroxyl flavonoids to produce 3′,4′-diydroxylated flavanones,dihydroflavonols,flavonols,and flavones.HmGAT(flavonoid 3-O-galactosyltransferase)would transform flavonols into the corresponding 3-O-galactosides,including hyperoside.The parallel hyperoside biosynthesis routes were thus depicted,one of which was successfully reconstructed in Escherichia coli BL21(DE3)by feeding naringenin,resulting in a hyperoside yield of 25 mg/l.Overall,this research not only helped us understand the interior catalytic mechanism of hyperoside in H.monogynum concerning flower development and bioactivity,but also provided valuable insights into these enzyme families.
文摘When discovering the potential of canards flying in 4-dimensional slow-fast system with a bifurcation parameter, the key notion “symmetry” plays an important role. It is of one parameter on slow vector field. Then, it should be determined to introduce parameters to all slow/fast vectors. It is, however, there might be no way to explore for another potential in this system, because the geometrical structure is quite different from the system with one parameter. Even in this system, the “symmetry” is also useful to obtain the potentials classified by R. Thom. In this paper, via the coordinates changing, the possible way to explore for the potential will be shown. As it is analyzed on “hyper finite time line”, or done by using “non-standard analysis”, it is called “Hyper Catastrophe”. In the slow-fast system which includes a very small parameter , it is difficult to do precise analysis. Thus, it is useful to get the orbits as a singular limit. When trying to do simulations, it is also faced with difficulty due to singularity. Using very small time intervals corresponding small , we shall overcome the difficulty, because the difference equation on the small time interval adopts the standard differential equation. These small intervals are defined on hyper finite number N, which is nonstandard. As and the intervals are linked to use 1/N, the simulation should be done exactly.
基金supported by the National Science Foundation of China(NSFC)(52075162)the Program of New and High-Tech Industry of Hunan Province(2020GK2015 and 2021GK4014)+5 种基金the Excellent Youth Fund of Hunan Province(2021JJ20018)the Key Program of Guangdong(2020B0101040002)the Joint Fund of the Ministry of Education(Young Talents)the Natural Science Foundation of Changsha(kq2007026)the Tianjin Enterprise Science and Technology Commissioner Project(19JCTPJC56200)the Engineering Physics and Science Research Council of the United Kingdom(EPSRC EP/P018998/1).
文摘Surface acoustic wave (SAW) technology has been extensively explored for wireless communication, sensors, microfluidics, photonics, and quantum information processing. However, due to fabrication issues, the frequencies of SAW devices are typically limited to within a few gigahertz, which severely restricts their applications in 5G communication, precision sensing, photonics, and quantum control. To solve this critical problem, we propose a hybrid strategy that integrates a nanomanufacturing process (i.e., nanolithography) with a LiNbO_(3)/SiO_(2)/SiC heterostructure and successfully achieve a record-breaking frequency of about 44 GHz for SAW devices, in addition to large electromechanical coupling coefficients of up to 15.7%. We perform a theoretical analysis and identify the guided higher order wave modes generated on these slow-on-fast SAW platforms. To demonstrate the superior sensing performance of the proposed ultra-high-frequency SAW platforms, we perform micro-mass sensing and obtain an extremely high sensitivity of approximately 33151.9 MHz·mm2·μg−1, which is about 1011 times higher than that of a conventional quartz crystal microbalance (QCM) and about 4000 times higher than that of a conventional SAW device with a frequency of 978 MHz.
文摘Due to its complexity and involvement of numerous stakeholders,the pharmaceutical supply chain presents many challenges that companies must overcome to deliver necessary medications to patients efficiently.The pharmaceutical supply chain poses different challenging issues,encompasses supply chain visibility,cold-chain shipping,drug counterfeiting,and rising prescription drug prices,which can considerably surge out-of-pocket patient costs.Blockchain(BC)offers the technical base for such a scheme,as it could track legitimate drugs and avoid fake circulation.The designers presented the procedure of BC with fabric for creating a secured drug supplychain management(DSCM)method.With this motivation,the study presents a new blockchain with optimal deep learning-enabled DSCM and recommendation scheme(BCODL-DSCMRS)for Pharmaceutical Industries.Firstly,Hyperledger fabric is used for DSC management,enabling effective tracking processes in the smart pharmaceutical industry.In addition,a hybrid deep belief network(HDBN)model is used to suggest the best or top-rated medicines to healthcare providers and consumers.The spotted hyena optimizer(SHO)algorithm is used to optimize the performance of the HDBN model.The design of the HSO algorithm for tuning the HDBN model demonstrates the novelty of the work.The presented model is tested on the UCI repository’s open-access drug reviews database.
文摘In the Digital World scenario,the confidentiality of information in video transmission plays an important role.Chaotic systems have been shown to be effective for video signal encryption.To improve video transmission secrecy,compressive encryption method is proposed to accomplish compression and encryption based on fractional order hyper chaotic system that incorporates Compressive Sensing(CS),pixel level,bit level scrambling and nucleotide Sequences operations.The measurement matrix generates by the fractional order hyper chaotic system strengthens the efficiency of the encryption process.To avoid plain text attack,the CS measurement is scrambled to its pixel level,bit level scrambling decreases the similarity between the adjacent measurements and the nucleotide sequence operations are done on the scrambled bits,increasing the encryption.Two stages are comprised in the reconstruction technique,the first stage uses the intra-frame similarity and offers robust preliminary retrieval for each frame,and the second stage iteratively improves the efficiency of reconstruction by integrating inter frame Multi Hypothesis(MH)estimation and weighted residual sparsity modeling.In each iteration,the residual coefficient weights are modified using a mathematical approach based on the MH predictions,and the Split Bregman iteration algorithm is defined to resolve weighted l1 regularization.Experimental findings show that the proposed algorithm provides good compression of video coupled with an efficient encryption method that is resistant to multiple attacks.
