[Objective] The aim was to study the effects of the extract from Hypericum perforatum on highly pathogenic Avian Influenza Virus(HPAIV)in vivo.[Method] Chickens infected with H5N1 virus were treated with the extract f...[Objective] The aim was to study the effects of the extract from Hypericum perforatum on highly pathogenic Avian Influenza Virus(HPAIV)in vivo.[Method] Chickens infected with H5N1 virus were treated with the extract from H.perforatum for 4 d.The virus was then detected by hemoagglutination(HA)test and reverse transcription polymerase chain reaction(RT-PCR).[Result] No H5N1 virus could be detected at the 7th d when the chickens were treated with 0.2 or 0.1 g/(kg·d)of the extract from H.perforatum.However,the virus could be detected in other chickens without the extract from HPE treatment.[Conclusion] The extract from H.perforatum might be a potential drug candidate to control infection of H5N1 subtype AIV in chickens.展开更多
利用HPLC/DAD-ESI(-)MS联用技术对植物长萼小连翘(Hypericum erectum subsp. longisepalum L. H. Wu et D. P. Yang)中主要化学成分进行了分离分析,初步鉴定出金丝桃素、伪金丝桃素、原伪金丝桃素、绿原酸、芦丁、金丝桃苷、槲皮素等12...利用HPLC/DAD-ESI(-)MS联用技术对植物长萼小连翘(Hypericum erectum subsp. longisepalum L. H. Wu et D. P. Yang)中主要化学成分进行了分离分析,初步鉴定出金丝桃素、伪金丝桃素、原伪金丝桃素、绿原酸、芦丁、金丝桃苷、槲皮素等12种黄酮类、酚酸类和金丝桃素类等化学成分。展开更多
This study employed the α-glucosidase inhibitory activity model as an anti-diabetic assay and implemented a bioactivity-guided isolation strategy to identify novel natural compounds with potential therapeutic propert...This study employed the α-glucosidase inhibitory activity model as an anti-diabetic assay and implemented a bioactivity-guided isolation strategy to identify novel natural compounds with potential therapeutic properties.Hypericum sampsonii was investigated,leading to the isolation of two highly modified seco-polycyclic polyprenylated acylphloroglucinols(PPAPs)(1 and 2),eight phenolic derivatives(3-10),and four terpene derivatives(11-14).The structures of compounds 1 and 2,featuring an unprecedented octahydro-2H-chromen-2-one ring system,were fully characterized using extensive spectroscopic data and quantum chemistry calculations.Six compounds(1,5-7,9,and 14)exhibited potential inhibitory effects against α-glucosidase,with IC_(50) values ranging from 0.050±0.0016 to 366.70±11.08μg·mL^(-1).Notably,compound 5(0.050±0.0016μg·mL^(-1))was identified as the most potential α-glucosidase inhibitor,with an inhibitory effect about 6900 times stronger than the positive control,acarbose(IC_(50)=346.63±15.65μg·mL^(-1)).A docking study was conducted to predict molecular interactions between two compounds(1 and 5)and α-glucosidase,and the hypothetical biosynthetic pathways of the two unprecedented seco-PPAPs were proposed.展开更多
The total flavonoid contents in cultural materials and natural plant of Hypericum sampsonii Hance were analysed and comparedwith that in cultural materials of H. perforatum Linn. The results showed that the total flav...The total flavonoid contents in cultural materials and natural plant of Hypericum sampsonii Hance were analysed and comparedwith that in cultural materials of H. perforatum Linn. The results showed that the total flavonoid contents reached 5.416%-5.868% in H.sampsonii calli cultured for 30 d, which werehigher than that ofnatural plantof thisspecies (4.325%-4.527%) and approached to cultural materials of H. perforatum .展开更多
基金Supported by Hundred Talent Program of Chinese Academy of Sciences(O861010ZY0)Key Technologies R&D Program(2004BA519A26)
文摘[Objective] The aim was to study the effects of the extract from Hypericum perforatum on highly pathogenic Avian Influenza Virus(HPAIV)in vivo.[Method] Chickens infected with H5N1 virus were treated with the extract from H.perforatum for 4 d.The virus was then detected by hemoagglutination(HA)test and reverse transcription polymerase chain reaction(RT-PCR).[Result] No H5N1 virus could be detected at the 7th d when the chickens were treated with 0.2 or 0.1 g/(kg·d)of the extract from H.perforatum.However,the virus could be detected in other chickens without the extract from HPE treatment.[Conclusion] The extract from H.perforatum might be a potential drug candidate to control infection of H5N1 subtype AIV in chickens.
文摘利用HPLC/DAD-ESI(-)MS联用技术对植物长萼小连翘(Hypericum erectum subsp. longisepalum L. H. Wu et D. P. Yang)中主要化学成分进行了分离分析,初步鉴定出金丝桃素、伪金丝桃素、原伪金丝桃素、绿原酸、芦丁、金丝桃苷、槲皮素等12种黄酮类、酚酸类和金丝桃素类等化学成分。
基金supported by the National Natural Science Foundation of China(No.32270413,U1812403)the Science and Technology Department of Guizhou Province(No.QKH 2020-1Z076)+5 种基金the 13th batch of Outstanding Young Scientific and Technological Talents in Guizhou Province(No.QKHPTRC[2021]5633)Cultivation project of National Natural Science Foundation of Guizhou Medical University(No.19NSP003)the Education Department of Guizhou Province(No.QJH KY[2021]189)the Natural Science Foundation of Guizhou Province(No.QKHZYD[2022]4015)High-level Innovative Talents in Guizhou Province(Thousand Levels of Talent for Chunmao Yuan in 2018)“Light of the West”Talent Cultivation Program of Chinese Academy of Sciences for Chunmao Yuan(No.RZ[2020]82).
文摘This study employed the α-glucosidase inhibitory activity model as an anti-diabetic assay and implemented a bioactivity-guided isolation strategy to identify novel natural compounds with potential therapeutic properties.Hypericum sampsonii was investigated,leading to the isolation of two highly modified seco-polycyclic polyprenylated acylphloroglucinols(PPAPs)(1 and 2),eight phenolic derivatives(3-10),and four terpene derivatives(11-14).The structures of compounds 1 and 2,featuring an unprecedented octahydro-2H-chromen-2-one ring system,were fully characterized using extensive spectroscopic data and quantum chemistry calculations.Six compounds(1,5-7,9,and 14)exhibited potential inhibitory effects against α-glucosidase,with IC_(50) values ranging from 0.050±0.0016 to 366.70±11.08μg·mL^(-1).Notably,compound 5(0.050±0.0016μg·mL^(-1))was identified as the most potential α-glucosidase inhibitor,with an inhibitory effect about 6900 times stronger than the positive control,acarbose(IC_(50)=346.63±15.65μg·mL^(-1)).A docking study was conducted to predict molecular interactions between two compounds(1 and 5)and α-glucosidase,and the hypothetical biosynthetic pathways of the two unprecedented seco-PPAPs were proposed.
文摘The total flavonoid contents in cultural materials and natural plant of Hypericum sampsonii Hance were analysed and comparedwith that in cultural materials of H. perforatum Linn. The results showed that the total flavonoid contents reached 5.416%-5.868% in H.sampsonii calli cultured for 30 d, which werehigher than that ofnatural plantof thisspecies (4.325%-4.527%) and approached to cultural materials of H. perforatum .
