Hypergraph Regularized Deep Autoencoder for Unsupervised Unmixing Hyperspectral Images

Deep learning(DL)has shown its superior performance in dealing with various computer vision tasks in recent years.As a simple and effective DL model,autoencoder(AE)is popularly used to decompose hyperspectral images(HSIs)due to its powerful ability of feature extraction and data reconstruction.However,most existing AE-based unmixing algorithms usually ignore the spatial information of HSIs.To solve this problem,a hypergraph regularized deep autoencoder(HGAE)is proposed for unmixing.Firstly,the traditional AE architecture is specifically improved as an unsupervised unmixing framework.Secondly,hypergraph learning is employed to reformulate the loss function,which facilitates the expression of high-order similarity among locally neighboring pixels and promotes the consistency of their abundances.Moreover,L_(1/2)norm is further used to enhance abundances sparsity.Finally,the experiments on simulated data,real hyperspectral remote sensing images,and textile cloth images are used to verify that the proposed method can perform better than several state-of-the-art unmixing algorithms.

Automated Deep Learning Driven Crop Classification on Hyperspectral Remote Sensing Images

Hyperspectral remote sensing/imaging spectroscopy is a novel approach to reaching a spectrum from all the places of a huge array of spatial places so that several spectral wavelengths are utilized for making coherent images.Hyperspectral remote sensing contains acquisition of digital images from several narrow,contiguous spectral bands throughout the visible,Thermal Infrared(TIR),Near Infrared(NIR),and Mid-Infrared(MIR)regions of the electromagnetic spectrum.In order to the application of agricultural regions,remote sensing approaches are studied and executed to their benefit of continuous and quantitativemonitoring.Particularly,hyperspectral images(HSI)are considered the precise for agriculture as they can offer chemical and physical data on vegetation.With this motivation,this article presents a novel Hurricane Optimization Algorithm with Deep Transfer Learning Driven Crop Classification(HOADTL-CC)model onHyperspectralRemote Sensing Images.The presentedHOADTL-CC model focuses on the identification and categorization of crops on hyperspectral remote sensing images.To accomplish this,the presentedHOADTL-CC model involves the design ofHOAwith capsule network(CapsNet)model for generating a set of useful feature vectors.Besides,Elman neural network(ENN)model is applied to allot proper class labels into the input HSI.Finally,glowworm swarm optimization(GSO)algorithm is exploited to fine tune the ENNparameters involved in this article.The experimental result scrutiny of the HOADTL-CC method can be tested with the help of benchmark dataset and the results are assessed under distinct aspects.Extensive comparative studies stated the enhanced performance of the HOADTL-CC model over recent approaches with maximum accuracy of 99.51%.

A Novel Fuzzy Inference System-Based Endmember Extraction in Hyperspectral Images

Spectral unmixing helps to identify different components present in the spectral mixtures which occur in the uppermost layer of the area owing to the low spatial resolution of hyperspectral images.Most spectral unmixing methods are globally based and do not consider the spectral variability among its endmembers that occur due to illumination,atmospheric,and environmental conditions.Here,endmember bundle extraction plays a major role in overcoming the above-mentioned limitations leading to more accurate abundance fractions.Accordingly,a two-stage approach is proposed to extract endmembers through endmember bundles in hyperspectral images.The divide and conquer method is applied as the first step in subset images with only the non-redundant bands to extract endmembers using the Vertex Component Analysis(VCA)and N-FINDR algorithms.A fuzzy rule-based inference system utilizing spectral matching parameters is proposed in the second step to categorize endmembers.The endmember with the minimum error is chosen as the final endmember in each specific category.The proposed method is simple and automatically considers endmember variability in hyperspectral images.The efficiency of the proposed method is evaluated using two real hyperspectral datasets.The average spectral angle and abundance angle are used to analyze the performance measures.

Hybrid Multi-Strategy Aquila Optimization with Deep Learning Driven Crop Type Classification on Hyperspectral Images

Hyperspectral imaging instruments could capture detailed spatial information and rich spectral signs of observed scenes.Much spatial information and spectral signatures of hyperspectral images(HSIs)present greater potential for detecting and classifying fine crops.The accurate classification of crop kinds utilizing hyperspectral remote sensing imaging(RSI)has become an indispensable application in the agricultural domain.It is significant for the prediction and growth monitoring of crop yields.Amongst the deep learning(DL)techniques,Convolution Neural Network(CNN)was the best method for classifying HSI for their incredible local contextual modeling ability,enabling spectral and spatial feature extraction.This article designs a Hybrid Multi-Strategy Aquila Optimization with a Deep Learning-Driven Crop Type Classification(HMAODL-CTC)algorithm onHSI.The proposed HMAODL-CTC model mainly intends to categorize different types of crops on HSI.To accomplish this,the presented HMAODL-CTC model initially carries out image preprocessing to improve image quality.In addition,the presented HMAODL-CTC model develops dilated convolutional neural network(CNN)for feature extraction.For hyperparameter tuning of the dilated CNN model,the HMAO algorithm is utilized.Eventually,the presented HMAODL-CTC model uses an extreme learning machine(ELM)model for crop type classification.A comprehensive set of simulations were performed to illustrate the enhanced performance of the presented HMAODL-CTC algorithm.Extensive comparison studies reported the improved performance of the presented HMAODL-CTC algorithm over other compared methods.

Hyperspectral Images-Based Crop Classification Scheme for Agricultural Remote Sensing

Hyperspectral imaging is gaining a significant role in agricultural remote sensing applications.Its data unit is the hyperspectral cube which holds spatial information in two dimensions while spectral band information of each pixel in the third dimension.The classification accuracy of hyperspectral images(HSI)increases significantly by employing both spatial and spectral features.For this work,the data was acquired using an airborne hyperspectral imager system which collected HSI in the visible and near-infrared(VNIR)range of 400 to 1000 nm wavelength within 180 spectral bands.The dataset is collected for nine different crops on agricultural land with a spectral resolution of 3.3 nm wavelength for each pixel.The data was cleaned from geometric distortions and stored with the class labels and annotations of global localization using the inertial navigation system.In this study,a unique pixel-based approach was designed to improve the crops'classification accuracy by using the edge-preserving features(EPF)and principal component analysis(PCA)in conjunction.The preliminary processing generated the high-dimensional EPF stack by applying the edge-preserving filters on acquired HSI.In the second step,this high dimensional stack was treated with the PCA for dimensionality reduction without losing significant spectral information.The resultant feature space(PCA-EPF)demonstrated enhanced class separability for improved crop classification with reduced dimensionality and computational cost.The support vector machines classifier was employed for multiclass classification of target crops using PCA-EPF.The classification performance evaluation was measured in terms of individual class accuracy,overall accuracy,average accuracy,and Cohen kappa factor.The proposed scheme achieved greater than 90%results for all the performance evaluation metrics.The PCA-EPF proved to be an effective attribute for crop classification using hyperspectral imaging in the VNIR range.The proposed scheme is well-suited for practical applications of crops and landfill estimations using agricultural remote sensing methods.

Topology and Semantic Information Fusion Classification Network Based on Hyperspectral Images of Chinese Herbs

Most methods for classifying hyperspectral data only consider the local spatial relation-ship among samples,ignoring the important non-local topological relationship.However,the non-local topological relationship is better at representing the structure of hyperspectral data.This paper proposes a deep learning model called Topology and semantic information fusion classification network(TSFnet)that incorporates a topology structure and semantic information transmis-sion network to accurately classify traditional Chinese medicine in hyperspectral images.TSFnet uses a convolutional neural network(CNN)to extract features and a graph convolution network(GCN)to capture potential topological relationships among different types of Chinese herbal medicines.The results show that TSFnet outperforms other state-of-the-art deep learning classification algorithms in two different scenarios of herbal medicine datasets.Additionally,the proposed TSFnet model is lightweight and can be easily deployed for mobile herbal medicine classification.

Hyperspectral Image Super-Resolution Network Based on Reinforcing Inter-Spectral Incremental Information

Hyperspectral images typically have high spectral resolution but low spatial resolution,which impacts the reliability and accuracy of subsequent applications,for example,remote sensingclassification and mineral identification.But in traditional methods via deep convolution neural net-works,indiscriminately extracting and fusing spectral and spatial features makes it challenging toutilize the differentiated information across adjacent spectral channels.Thus,we proposed a multi-branch interleaved iterative upsampling hyperspectral image super-resolution reconstruction net-work(MIIUSR)to address the above problems.We reinforce spatial feature extraction by integrat-ing detailed features from different receptive fields across adjacent channels.Furthermore,we pro-pose an interleaved iterative upsampling process during the reconstruction stage,which progres-sively fuses incremental information among adjacent frequency bands.Additionally,we add twoparallel three dimensional(3D)feature extraction branches to the backbone network to extractspectral and spatial features of varying granularity.We further enhance the backbone network’sconstruction results by leveraging the difference between two dimensional(2D)channel-groupingspatial features and 3D multi-granularity features.The results obtained by applying the proposednetwork model to the CAVE test set show that,at a scaling factor of×4,the peak signal to noiseratio,spectral angle mapping,and structural similarity are 37.310 dB,3.525 and 0.9438,respec-tively.Besides,extensive experiments conducted on the Harvard and Foster datasets demonstratethe superior potential of the proposed model in hyperspectral super-resolution reconstruction.

Method for evaluation of geological strength index of carbonate cliff rocks:Coupled hyperspectral-digital borehole image technique

The deterioration of unstable rock mass raised interest in evaluating rock mass quality.However,the traditional evaluation method for the geological strength index(GSI)primarily emphasizes the rock structure and characteristics of discontinuities.It ignores the influence of mineral composition and shows a deficiency in assessing the integrity coefficient.In this context,hyperspectral imaging and digital panoramic borehole camera technologies are applied to analyze the mineral content and integrity of rock mass.Based on the carbonate mineral content and fissure area ratio,the strength reduction factor and integrity coefficient are calculated to improve the GSI evaluation method.According to the results of mineral classification and fissure identification,the strength reduction factor and integrity coefficient increase with the depth of rock mass.The rock mass GSI calculated by the improved method is mainly concentrated between 40 and 60,which is close to the calculation results of the traditional method.The GSI error rates obtained by the two methods are mostly less than 10%,indicating the rationality of the hyperspectral-digital borehole image coupled evaluation method.Moreover,the sensitivity of the fissure area ratio(Sr)to GSI is greater than that of the strength reduction factor(a),which means the proposed GSI is suitable for rocks with significant fissure development.The improved method reduces the influence of subjective factors and provides a reliable index for the deterioration evaluation of rock mass.

Batch Active Learning for Multispectral and Hyperspectral Image Segmentation Using Similarity Graphs

Graph learning,when used as a semi-supervised learning(SSL)method,performs well for classification tasks with a low label rate.We provide a graph-based batch active learning pipeline for pixel/patch neighborhood multi-or hyperspectral image segmentation.Our batch active learning approach selects a collection of unlabeled pixels that satisfy a graph local maximum constraint for the active learning acquisition function that determines the relative importance of each pixel to the classification.This work builds on recent advances in the design of novel active learning acquisition functions(e.g.,the Model Change approach in arXiv:2110.07739)while adding important further developments including patch-neighborhood image analysis and batch active learning methods to further increase the accuracy and greatly increase the computational efficiency of these methods.In addition to improvements in the accuracy,our approach can greatly reduce the number of labeled pixels needed to achieve the same level of the accuracy based on randomly selected labeled pixels.

Quantification of the adulteration concentration of palm kernel oil in virgin coconut oil using near-infrared hyperspectral imaging

The adulteration concentration of palm kernel oil(PKO)in virgin coconut oil(VCO)was quantified using near-infrared(NIR)hyperspectral imaging.Nowadays,some VCO is adulterated with lower-priced PKO to reduce production costs,which diminishes the quality of the VCO.This study used NIR hyperspectral imaging in the wavelength region 900-1,650 nm to create a quantitative model for the detection of PKO contaminants(0-100%)in VCO and to develop predictive mapping.The prediction equation for the adulteration of VCO with PKO was constructed using the partial least squares regression method.The best predictive model was pre-processed using the standard normal variate method,and the coefficient of determination of prediction was 0.991,the root mean square error of prediction was 2.93%,and the residual prediction deviation was 10.37.The results showed that this model could be applied for quantifying the adulteration concentration of PKO in VCO.The prediction adulteration concentration mapping of VCO with PKO was created from a calibration model that showed the color level according to the adulteration concentration in the range of 0-100%.NIR hyperspectral imaging could be clearly used to quantify the adulteration of VCO with a color level map that provides a quick,accurate,and non-destructive detection method.

Improving Generalization for Hyperspectral Image Classification:The Impact of Disjoint Sampling on Deep Models

Disjoint sampling is critical for rigorous and unbiased evaluation of state-of-the-art(SOTA)models e.g.,Attention Graph and Vision Transformer.When training,validation,and test sets overlap or share data,it introduces a bias that inflates performance metrics and prevents accurate assessment of a model’s true ability to generalize to new examples.This paper presents an innovative disjoint sampling approach for training SOTA models for the Hyperspectral Image Classification(HSIC).By separating training,validation,and test data without overlap,the proposed method facilitates a fairer evaluation of how well a model can classify pixels it was not exposed to during training or validation.Experiments demonstrate the approach significantly improves a model’s generalization compared to alternatives that include training and validation data in test data(A trivial approach involves testing the model on the entire Hyperspectral dataset to generate the ground truth maps.This approach produces higher accuracy but ultimately results in low generalization performance).Disjoint sampling eliminates data leakage between sets and provides reliable metrics for benchmarking progress in HSIC.Disjoint sampling is critical for advancing SOTA models and their real-world application to large-scale land mapping with Hyperspectral sensors.Overall,with the disjoint test set,the performance of the deep models achieves 96.36%accuracy on Indian Pines data,99.73%on Pavia University data,98.29%on University of Houston data,99.43%on Botswana data,and 99.88%on Salinas data.

Hyperspectral image super resolution using deep internal and self-supervised learning

By automatically learning the priors embedded in images with powerful modelling ca-pabilities,deep learning-based algorithms have recently made considerable progress in reconstructing the high-resolution hyperspectral(HR-HS)image.With previously collected large-amount of external data,these methods are intuitively realised under the full supervision of the ground-truth data.Thus,the database construction in merging the low-resolution(LR)HS(LR-HS)and HR multispectral(MS)or RGB image research paradigm,commonly named as HSI SR,requires collecting corresponding training triplets:HR-MS(RGB),LR-HS and HR-HS image simultaneously,and often faces dif-ficulties in reality.The learned models with the training datasets collected simultaneously under controlled conditions may significantly degrade the HSI super-resolved perfor-mance to the real images captured under diverse environments.To handle the above-mentioned limitations,the authors propose to leverage the deep internal and self-supervised learning to solve the HSI SR problem.The authors advocate that it is possible to train a specific CNN model at test time,called as deep internal learning(DIL),by on-line preparing the training triplet samples from the observed LR-HS/HR-MS(or RGB)images and the down-sampled LR-HS version.However,the number of the training triplets extracted solely from the transformed data of the observation itself is extremely few particularly for the HSI SR tasks with large spatial upscale factors,which would result in limited reconstruction performance.To solve this problem,the authors further exploit deep self-supervised learning(DSL)by considering the observations as the unlabelled training samples.Specifically,the degradation modules inside the network were elaborated to realise the spatial and spectral down-sampling procedures for transforming the generated HR-HS estimation to the high-resolution RGB/LR-HS approximation,and then the reconstruction errors of the observations were formulated for measuring the network modelling performance.By consolidating the DIL and DSL into a unified deep framework,the authors construct a more robust HSI SR method without any prior training and have great potential of flexible adaptation to different settings per obser-vation.To verify the effectiveness of the proposed approach,extensive experiments have been conducted on two benchmark HS datasets,including the CAVE and Harvard datasets,and demonstrate the great performance gain of the proposed method over the state-of-the-art methods.

Multiscale Fusion Transformer Network for Hyperspectral Image Classification

Convolutional neural network(CNN)has excellent ability to model locally contextual information.However,CNNs face challenges for descripting long-range semantic features,which will lead to relatively low classification accuracy of hyperspectral images.To address this problem,this article proposes an algorithm based on multiscale fusion and transformer network for hyperspectral image classification.Firstly,the low-level spatial-spectral features are extracted by multi-scale residual structure.Secondly,an attention module is introduced to focus on the more important spatialspectral information.Finally,high-level semantic features are represented and learned by a token learner and an improved transformer encoder.The proposed algorithm is compared with six classical hyperspectral classification algorithms on real hyperspectral images.The experimental results show that the proposed algorithm effectively improves the land cover classification accuracy of hyperspectral images.

Hyperspectral remote sensing identification of marine oil spills and emulsions using feature bands and double-branch dual-attention mechanism network

The accurate identification of marine oil spills and their emulsions is of great significance for emergency response to oil spill pollution.The selection of characteristic bands with strong separability helps to realize the rapid calculation of data on aircraft or in orbit,which will improve the timeliness of oil spill emergency monitoring.At the same time,the combination of spectral and spatial features can improve the accuracy of oil spill monitoring.Two ground-based experiments were designed to collect measured airborne hyperspectral data of crude oil and its emulsions,for which the multiscale superpixel level group clustering framework(MSGCF)was used to select spectral feature bands with strong separability.In addition,the double-branch dual-attention(DBDA)model was applied to identify crude oil and its emulsions.Compared with the recognition results based on original hyperspectral images,using the feature bands determined by MSGCF improved the recognition accuracy,and greatly shortened the running time.Moreover,the characteristic bands for quantifying the volume concentration of water-in-oil emulsions were determined,and a quantitative inversion model was constructed and applied to the AVIRIS image of the deepwater horizon oil spill event in 2010.This study verified the effectiveness of feature bands in identifying oil spill pollution types and quantifying concentration,laying foundation for rapid identification and quantification of marine oil spills and their emulsions on aircraft or in orbit.

A Spectral Convolutional Neural Network Model Based on Adaptive Fick’s Law for Hyperspectral Image Classification

Hyperspectral image classification stands as a pivotal task within the field of remote sensing,yet achieving highprecision classification remains a significant challenge.In response to this challenge,a Spectral Convolutional Neural Network model based on Adaptive Fick’s Law Algorithm(AFLA-SCNN)is proposed.The Adaptive Fick’s Law Algorithm(AFLA)constitutes a novel metaheuristic algorithm introduced herein,encompassing three new strategies:Adaptive weight factor,Gaussian mutation,and probability update policy.With adaptive weight factor,the algorithmcan adjust theweights according to the change in the number of iterations to improve the performance of the algorithm.Gaussianmutation helps the algorithm avoid falling into local optimal solutions and improves the searchability of the algorithm.The probability update strategy helps to improve the exploitability and adaptability of the algorithm.Within the AFLA-SCNN model,AFLA is employed to optimize two hyperparameters in the SCNN model,namely,“numEpochs”and“miniBatchSize”,to attain their optimal values.AFLA’s performance is initially validated across 28 functions in 10D,30D,and 50D for CEC2013 and 29 functions in 10D,30D,and 50D for CEC2017.Experimental results indicate AFLA’s marked performance superiority over nine other prominent optimization algorithms.Subsequently,the AFLA-SCNN model was compared with the Spectral Convolutional Neural Network model based on Fick’s Law Algorithm(FLA-SCNN),Spectral Convolutional Neural Network model based on Harris Hawks Optimization(HHO-SCNN),Spectral Convolutional Neural Network model based onDifferential Evolution(DE-SCNN),SpectralConvolutionalNeuralNetwork(SCNN)model,and SupportVector Machines(SVM)model using the Indian Pines dataset and PaviaUniversity dataset.The experimental results show that the AFLA-SCNN model outperforms other models in terms of Accuracy,Precision,Recall,and F1-score on Indian Pines and Pavia University.Among them,the Accuracy of the AFLA-SCNN model on Indian Pines reached 99.875%,and the Accuracy on PaviaUniversity reached 98.022%.In conclusion,our proposed AFLA-SCNN model is deemed to significantly enhance the precision of hyperspectral image classification.

Non-Negative Minimum Volume Factorization (NMVF) for Hyperspectral Images (HSI) Unmixing: A Hybrid Approach

Spectral unmixing is essential for exploitation of remotely senseddata of Hyperspectral Images (HSI). It amounts to the identification of a position of spectral signatures that are pure and therefore called end members andtheir matching fractional, draft rules abundances for every pixel in HSI. Thispaper aims to unmix hyperspectral data using the minimal volume methodof elementary scrutiny. Moreover, the problem of optimization is solved bythe implementation of the sequence of small problems that are constrainedquadratically. The hard constraint in the final step for the abundance fractionis then replaced with a loss function of hinge type that accounts for outlinersand noise. Existing algorithms focus on estimating the endmembers (Ems)enumeration in a sight, discerning of spectral signs of EMs, besides assessmentof fractional profusion for every EM in every pixel of a sight. Nevertheless, allthe stages are performed by only a few algorithms in the process of hyperspectral unmixing. Therefore, the Non-negative Minimum Volume Factorization(NMVF) algorithm is further extended by fusing it with the nonnegativematrix of robust collaborative factorization that aims to perform all the threeunmixing chain steps for hyperspectral images. The major contributions ofthis article are in this manner: (A) it performs Simplex analysis of minimum volume for hyperspectral images with unsupervised linear unmixing isemployed. (B) The simplex analysis method is configured with an exaggeratedform of the elementary which is delivered by vertical component analysis(VCA). (C) The inflating factor is chosen carefully inactivating the constraintsin a large majority for relating to the source fractions abundance that speedsup the algorithm. (D) The final step is making simplex analysis method robustto outliners as well as noise that replaces the profusion element positivity hardrestraint by a hinge kind soft restraint, preserving the local minima havinggood quality. (E) The matrix factorization method is applied that is capable ofperforming the three major phases of the hyperspectral separation sequence.The anticipated approach can find application in a scenario where the endmembers are known in advance, however, it assumes that the endmemberscount is corresponding to an overestimated value. The proposed method isdifferent from other conventional methods as it begins with the overestimationof the count of endmembers wherein removing the endmembers that areredundant by the means of collaborative regularization. As demonstrated bythe experimental results, proposed approach yields competitive performancecomparable with widely used methods.

Prediction of Fresh Pork Quality using Hyperspectral Scattering Imaging(HSI) Technique

In this study, fresh pork tenderness, drip-loss, pH value and color parameters （ CIE, a ＊ , b ＊ and L ＊ values） were simultaneously predicted using hyperspectral scattering imaging （HSI） technique. The hyperspectral scattering images of dO fresh pork samples were collected at the wavelength of 400 -I 100 nm, and the scattering profiles were fitted via Lorontzian distribution （ LD ） function to give three parameters a （ asymptotic value ）, b （peak value ） and c （ full width at b/2）. Stepwise discrimination was performed to determine the optimal wavelengths combinations. The LD parameters combinations （a, b and c） of optimal wavelengths were used to establish multi-linear regression （MLR） models to predict the pork attributes. The models were able to predict pork with high correlation coefficients of 0.92 for drip-loss, 0.94, 0.92 and 0.98 respectively for color parameters （ a ＊ , b＊ and L ＊ ）, and for tenderness and pH value the models gave the correlation coefficients of 0.69 and 0.76, respectively. These results showed that the hyperspectral scattering technique was capable of predicting quality parameters of perk. The study provides an efficient means for rapid and nondestructive determination of pork quality simultaneously.

Classification of hyperspectral remote sensing images based on simulated annealing genetic algorithm and multiple instance learning

A hybrid feature selection and classification strategy was proposed based on the simulated annealing genetic algonthrn and multiple instance learning （MIL）. The band selection method was proposed from subspace decomposition, which combines the simulated annealing algorithm with the genetic algorithm in choosing different cross-over and mutation probabilities, as well as mutation individuals. Then MIL was combined with image segmentation, clustering and support vector machine algorithms to classify hyperspectral image. The experimental results show that this proposed method can get high classification accuracy of 93.13% at small training samples and the weaknesses of the conventional methods are overcome.

Low complexity DCT-based distributed source coding with Gray code for hyperspectral images

To compress hyperspectral images, a low complexity discrete cosine transform （DCT）-based distributed source coding （DSC） scheme with Gray code is proposed. Unlike most of the existing DSC schemes, which utilize transform in spatial domain, the proposed algorithm applies transform in spectral domain. Set-partitioning-based approach is applied to reorganize DCT coefficients into waveletlike tree structure and extract the sign, refinement, and significance bitplanes. The extracted refinement bits are Gray encoded. Because of the dependency along the line dimension of hyperspectral images, low density paritycheck-（LDPC）-based Slepian-Wolf coder is adopted to implement the DSC strategy. Experimental results on airborne visible/infrared imaging spectrometer （AVIRIS） dataset show that the proposed paradigm achieves up to 6 dB improvement over DSC-based coders which apply transform in spatial domain, with significantly reduced computational complexity and memory storage.

Classification of hyperspectral images for detection of hepatic carcinoma cells based on spectral-spatial features of nucleus

A distinguishing characteristic of normal and cancer cells is the difference in their nuclear chromatin content and distribution.This difference can be revealed by the transmission spectra of nuclei stained with a pH-sensitive stain.Here,we used hematoxylin-eosin(HE)to stain hepatic carcinoma tissues and obtained spectral-spatial data from their nuclei using hyper-spectral microscopy.The transmission spectra of the nuclei were then used to train a support vector machine(SVM)model for cell classification.Especially,we found that the chromatin distribution in cancer cells is more uniform,because of which the correlation coefficients for the spectra at different points in their nuclei are higher.Consequently,we exploited this feature to improve the SVM model.The sensitivity and specificity for the identification of cancer cells could be increased to 99%and 98%,respectively.We also designed an image-processing method for the extraction of information from cell nuclei to automate the identification process.