We selected healthy Pinus massioniana for pine wood nematode inoculation experiments to get the spectral reflectance of healthy and infected Pinus mas- sioniana in different infection stages via a ground spectrometer ...We selected healthy Pinus massioniana for pine wood nematode inoculation experiments to get the spectral reflectance of healthy and infected Pinus mas- sioniana in different infection stages via a ground spectrometer （ wavelength in 350 - 2 500 nm）, and analyzed the changes in chlorophyll content at various periods. The original spectral reflectance of healthy and infected P. massoniana was significantly different in the middle and late infection stages, and the reflection peak and absorption valley in visible light region and near infrared region gradually weakened and even disappeared to a straight line. There was significant correlation rela- tionship between chlorophyll content of infected plants and spectral reflectance at the wavelength of 1 405 nm, and the quantitative inversion model of chlorophyll content was correspondingly established as follows： Car = - 1.74（X1~ ）2 ＋ 4. 72X1,~ - 0. 76. Through first-order derivative spectra at the wavelength of 593 nm, combined with quantitative inversion of the corresponding chlorophyll content, we can discriminate whether P. massoniana is infected by pine lt disease or not, especially in the early stages before disease features are visible to the naked eyes it has a good quantitative monitoring effect.展开更多
基金Supported by Special Fund for Scientific Research(Forestry)in the Public Interest(201304208)National Natural Science Foundation of China(31100414,31470579)+1 种基金General Program of Natural Science Research in Colleges and Universities in Jiangsu Province(11KJB220001)Advantage Discipline Construction Project of Colleges and Universities in Jiangsu Province
文摘We selected healthy Pinus massioniana for pine wood nematode inoculation experiments to get the spectral reflectance of healthy and infected Pinus mas- sioniana in different infection stages via a ground spectrometer （ wavelength in 350 - 2 500 nm）, and analyzed the changes in chlorophyll content at various periods. The original spectral reflectance of healthy and infected P. massoniana was significantly different in the middle and late infection stages, and the reflection peak and absorption valley in visible light region and near infrared region gradually weakened and even disappeared to a straight line. There was significant correlation rela- tionship between chlorophyll content of infected plants and spectral reflectance at the wavelength of 1 405 nm, and the quantitative inversion model of chlorophyll content was correspondingly established as follows： Car = - 1.74（X1~ ）2 ＋ 4. 72X1,~ - 0. 76. Through first-order derivative spectra at the wavelength of 593 nm, combined with quantitative inversion of the corresponding chlorophyll content, we can discriminate whether P. massoniana is infected by pine lt disease or not, especially in the early stages before disease features are visible to the naked eyes it has a good quantitative monitoring effect.
