There is no effective treatment for hemiplegia after hypertensive intracerebral hemorrhage.Considering that the branches of L4 nerve roots in the lumbar plexus root control the movement of the lower extremity anterior...There is no effective treatment for hemiplegia after hypertensive intracerebral hemorrhage.Considering that the branches of L4 nerve roots in the lumbar plexus root control the movement of the lower extremity anterior and posterior muscles,we investigated a potential method of nerve repair using the L4 nerve roots.Rat models of hindlimb hemiplegia after a hypertensive intracerebral hemorrhage were established by injecting autogenous blood into the posterior limb of internal capsule.The L4 nerve root on the healthy side of model rats was transferred and then anastomosed with the L4 nerve root on the affected side to drive the extensor and flexor muscles of the hindlimbs.We investigated whether this method can restore the flexible movement of the hindlimbs of paralyzed rats after hypertensive intracerebral hemorrhage.In a beam-walking test and ladder rung walking task,model rats exhibited an initial high number of slips,but improved in accuracy on the paretic side over time.At 17 weeks after surgery,rats gained approximately 58.2%accuracy from baseline performance and performed ankle motions on the paretic side.At 9 weeks after surgery,a retrograde tracing test showed a large number of fluoro-gold-labeled motoneurons in the left anterior horn of the spinal cord that supports the L4-to-L4 nerve roots.In addition,histological and ultramicrostructural findings showed axon regeneration of motoneurons in the anterior horn of the spinal cord.Electromyography and paw print analysis showed that denervated hindlimb muscles regained reliable innervation and walking coordination improved.These findings suggest that the L4-to-L4 nerve root transfer method for the treatment of hindlimb hemiplegia after hypertensive intracerebral hemorrhage can improve the locomotion of hindlimb major joints,particularly of the distal ankle.Findings from study support that the L4-to-L4 nerve root transfer method can effectively repair the hindlimb hemiplegia after hypertensive intracerebral hemorrhage.All animal experiments were approved by the Animal Ethics Committee of the First Affiliated Hospital of Nanjing Medical University(No.IACUC-1906009)in June 2019.展开更多
AIM: To study the effects of endotoxin on portalhemodynamic of normal and noncirrhotic portal hypertensiverats.METHODS: Normal rats were intraperitonealy injected with 0.1,0.25, 0.5, 1.0, 2.0, 4.0 mg@kg-1 of lipopolys...AIM: To study the effects of endotoxin on portalhemodynamic of normal and noncirrhotic portal hypertensiverats.METHODS: Normal rats were intraperitonealy injected with 0.1,0.25, 0.5, 1.0, 2.0, 4.0 mg@kg-1 of lipopolysaccharide( LPS ) respectively, portal vein ligation ( PVL ) andintrahepatic portal occlusion (IPO) rats as well as sham-operated rats were treated with an intraperitoneal injection of1.0 mg@kg-1 of LPS, the portal vein pressure(PVP), portalvenous flow(PVF), inferior vena cava pressure(IVCP) andportal vein resistance (PVR) were detected 4 hours afterinjection.RESULTS: PVF of the 5 groups of rats acceptingintraperitoneal injection of LPS were increased from 14.0 to18.0, 22.2, 266.2, 34.8, 39.66, 38.8 mL@min-1 4 hours afterinjection of LPS ( P ＜ 0. 01 ). PVP of the 4 groups of ratsaccepting more than 0.1 mg/kg@ b. w of LPS was increasedfrom 1.04 to1.25, 1.50, 1.80, 1.95, 2.05 kPa(P＜0.01). Theincrements of PVF and PVP were in a dose-dependentmanner of LPS. PVR of the 5 groups of rats was decreasedfrom 51 to 42,44,48,45,44,47 kPa@min@ L-1 ( P＜ 0.05) and nodosedependent manner wes observed. PVF of PVL, IPOand sham-operated rats increased from 22.66 to 32.8, 22.0 to28.0, 14.0 to 34.8 mL@min1 ( P＜ 0.01), and PVP increasedfrom 1.86 to 2.24, 1.74 to 1.95, 1.04 to 1.80 kPa(P＜0.01),PVR decreased from 71 to61, 67 to61, 52 to 44 kPa@min@ L-1after intraperitoneal injection of 1 mg@ kg-1 of LPS. Theincrements of PVF and PVP of PVL and IPO rats weresignificantly less than the sham-operated rats ( P ＜ 0. 01 ),There wes no significant difference between the amounts ofPVR decreased in the two groups of PHT model rats andsham-operated rats( P＞ 0.05) after intraperitoneal injection 1mg@kg1 of LPS.CONCLUSION: Endotoxin could prompt portal hypertensionof the normal and noncirrhotic portal hypertensive rats byincreasing portal blood flow mainly.展开更多
基金the National Natural Science Foundation of China,No.81171147(to LXL)“Key Medical Talents of Qiangwei Project”Research Foundation of Health Department of Jiangsu Province,No.ZDRCA2016010(to LXL)+3 种基金“Xingwei Project”Key Personal Medical Research Foundation of Health Department of Jiangsu Province,No.RC201156(to LXL)Jiangsu Province’s Key Discipline of Medicine,No.XK201117(to LXL)the Priority Academic Program Development of Jiangsu Higher Education Institutions,PAPD(to LXL)the Natural Science Foundation of Jiangsu Province,No.BK20171064(to BSH).
文摘There is no effective treatment for hemiplegia after hypertensive intracerebral hemorrhage.Considering that the branches of L4 nerve roots in the lumbar plexus root control the movement of the lower extremity anterior and posterior muscles,we investigated a potential method of nerve repair using the L4 nerve roots.Rat models of hindlimb hemiplegia after a hypertensive intracerebral hemorrhage were established by injecting autogenous blood into the posterior limb of internal capsule.The L4 nerve root on the healthy side of model rats was transferred and then anastomosed with the L4 nerve root on the affected side to drive the extensor and flexor muscles of the hindlimbs.We investigated whether this method can restore the flexible movement of the hindlimbs of paralyzed rats after hypertensive intracerebral hemorrhage.In a beam-walking test and ladder rung walking task,model rats exhibited an initial high number of slips,but improved in accuracy on the paretic side over time.At 17 weeks after surgery,rats gained approximately 58.2%accuracy from baseline performance and performed ankle motions on the paretic side.At 9 weeks after surgery,a retrograde tracing test showed a large number of fluoro-gold-labeled motoneurons in the left anterior horn of the spinal cord that supports the L4-to-L4 nerve roots.In addition,histological and ultramicrostructural findings showed axon regeneration of motoneurons in the anterior horn of the spinal cord.Electromyography and paw print analysis showed that denervated hindlimb muscles regained reliable innervation and walking coordination improved.These findings suggest that the L4-to-L4 nerve root transfer method for the treatment of hindlimb hemiplegia after hypertensive intracerebral hemorrhage can improve the locomotion of hindlimb major joints,particularly of the distal ankle.Findings from study support that the L4-to-L4 nerve root transfer method can effectively repair the hindlimb hemiplegia after hypertensive intracerebral hemorrhage.All animal experiments were approved by the Animal Ethics Committee of the First Affiliated Hospital of Nanjing Medical University(No.IACUC-1906009)in June 2019.
文摘AIM: To study the effects of endotoxin on portalhemodynamic of normal and noncirrhotic portal hypertensiverats.METHODS: Normal rats were intraperitonealy injected with 0.1,0.25, 0.5, 1.0, 2.0, 4.0 mg@kg-1 of lipopolysaccharide( LPS ) respectively, portal vein ligation ( PVL ) andintrahepatic portal occlusion (IPO) rats as well as sham-operated rats were treated with an intraperitoneal injection of1.0 mg@kg-1 of LPS, the portal vein pressure(PVP), portalvenous flow(PVF), inferior vena cava pressure(IVCP) andportal vein resistance (PVR) were detected 4 hours afterinjection.RESULTS: PVF of the 5 groups of rats acceptingintraperitoneal injection of LPS were increased from 14.0 to18.0, 22.2, 266.2, 34.8, 39.66, 38.8 mL@min-1 4 hours afterinjection of LPS ( P ＜ 0. 01 ). PVP of the 4 groups of ratsaccepting more than 0.1 mg/kg@ b. w of LPS was increasedfrom 1.04 to1.25, 1.50, 1.80, 1.95, 2.05 kPa(P＜0.01). Theincrements of PVF and PVP were in a dose-dependentmanner of LPS. PVR of the 5 groups of rats was decreasedfrom 51 to 42,44,48,45,44,47 kPa@min@ L-1 ( P＜ 0.05) and nodosedependent manner wes observed. PVF of PVL, IPOand sham-operated rats increased from 22.66 to 32.8, 22.0 to28.0, 14.0 to 34.8 mL@min1 ( P＜ 0.01), and PVP increasedfrom 1.86 to 2.24, 1.74 to 1.95, 1.04 to 1.80 kPa(P＜0.01),PVR decreased from 71 to61, 67 to61, 52 to 44 kPa@min@ L-1after intraperitoneal injection of 1 mg@ kg-1 of LPS. Theincrements of PVF and PVP of PVL and IPO rats weresignificantly less than the sham-operated rats ( P ＜ 0. 01 ),There wes no significant difference between the amounts ofPVR decreased in the two groups of PHT model rats andsham-operated rats( P＞ 0.05) after intraperitoneal injection 1mg@kg1 of LPS.CONCLUSION: Endotoxin could prompt portal hypertensionof the normal and noncirrhotic portal hypertensive rats byincreasing portal blood flow mainly.
