The polysaccharide was isolated from Hypnea pannosa which was grown in Okinawa, Japan. The yield of the polysaccharide was 17.2%, and the total carbohydrates, pyruvic acid, sulfuric acid and ash contents were 55.2%, 3...The polysaccharide was isolated from Hypnea pannosa which was grown in Okinawa, Japan. The yield of the polysaccharide was 17.2%, and the total carbohydrates, pyruvic acid, sulfuric acid and ash contents were 55.2%, 3.8%, 35.2% and 24.3%, respectively. 3,6-Anhydro-α-D-galactose, β-D-galactose, α-D-galactose and D-glucose were identified by liquid and thin-layer chromatography. Fourier transform infrared (FTIR) spectra of the polysaccharide resembled that of ι-carrageenan. From the <sup>1</sup>H- and <sup>13</sup>C-NMR spectra, 1,3-linked β-D-galactose, 1,4-linked anhydro-α-D-galactose, 1,4-linked α-D-galactose, 1,4-linked β-D-glucose and pyruvic acid (carboxyl acetal, methyl proton and methyl carbon) were assigned. Methylation analysis revealed terminal D-galactose 0.1 mol), 1,4-linked D-glucose (1.0 mol) and 1,2,3,4,6-linked D-galactose (3.7 mol) for native polysaccharide, and terminal D-galactose, 1,4-linked D-galactose (1.9 mol), 1,4-linked D-glucose (1.0 mol), 1,3- linked D-galactose (1.7 mol), and 1,3,4,6-linked D-galactose (0.3 mol) which substituted with pyruvate group at 4 and 6 positions for desulfated polysaccharide. The polysaccharide was the novel pyruvated glucogalactan sulfate, the structure of which was proposed.展开更多
Objective:To investigate some selected metabolic constituents of Hypnea valentiae(Turner)Montagne collected from the Red Sea coast of Sudan to evaluate the economic potential of the alga.Methods:Healthy thalli of the ...Objective:To investigate some selected metabolic constituents of Hypnea valentiae(Turner)Montagne collected from the Red Sea coast of Sudan to evaluate the economic potential of the alga.Methods:Healthy thalli of the alga under investigation were collected,thoroughly cleaned,air dried and milled.Authentic analytical methods were used to determine moisture,ash,protein lipid and carrageenan contents.Fatty acids profile was revealed with gas chromatography instrumentation.Testing of phytochemical groups of compounds was based on the development of colouration and precipitation upon addition of certain chemical reagents to the extracts.Results:The ash content of the alga[(40.3±0.2)%of dry weight]was comparatively the highest among the parameters tested.The protein,lipid,and carrageenan constituted(9.30±0.70)%,(6.50±0.34)%,and(33.70±0.01)%,respectively of the alga dry matter.Hypnea valentiae(Turner)Montagne from Sudan Red Sea coast composed of 12 fatty acids,8 of which were unsaturated fatty acids and 4 were saturated fatty acids.The major unsaturated fatty acids in the alga was the trans-isomer of linoleic acid and linolelaidic acid(36.32%)followed by palmitoleic acid(13.64%).The major saturated fatty acids was the heptadecanoic acid amounting to 19.10%of the total fatty acids.Phytochemically the alga contained alkaloids,flavonoids and tannins.Conclusions:This alga may represent a promising source of functional food and therapeutic metabolites.Further investigation and critical evaluation of the bioactivity of the phytochemical compounds is required to assure the therapeutic potential of this alga.展开更多
Two compositions,a pure ceramide and a sterol mixture,were isolated from the ethylacetate phase of ethanol extract of the Hypnea Charoides Lamx,collected from the South China Sea.The ceramide was identified by spectro...Two compositions,a pure ceramide and a sterol mixture,were isolated from the ethylacetate phase of ethanol extract of the Hypnea Charoides Lamx,collected from the South China Sea.The ceramide was identified by spectroscopic analysis as N-(2’hydroxytetracosyl)-1,3,4-trihydroxy-2-am ino-octadecane;The six sterols were detected by gas chromatography/mass spectrometry,and their structures were confirmed by spectroscopic analysis as Ergost-5,22-dien-3-ol,Ergost-5-en-3-ol,Stigmasta-5,22-dien-3-ol,Cholesta-5,22-dien-3-ol,Cholesta-5-en-3-ol,Stigmasta-5-en-3-ol.展开更多
文摘The polysaccharide was isolated from Hypnea pannosa which was grown in Okinawa, Japan. The yield of the polysaccharide was 17.2%, and the total carbohydrates, pyruvic acid, sulfuric acid and ash contents were 55.2%, 3.8%, 35.2% and 24.3%, respectively. 3,6-Anhydro-α-D-galactose, β-D-galactose, α-D-galactose and D-glucose were identified by liquid and thin-layer chromatography. Fourier transform infrared (FTIR) spectra of the polysaccharide resembled that of ι-carrageenan. From the <sup>1</sup>H- and <sup>13</sup>C-NMR spectra, 1,3-linked β-D-galactose, 1,4-linked anhydro-α-D-galactose, 1,4-linked α-D-galactose, 1,4-linked β-D-glucose and pyruvic acid (carboxyl acetal, methyl proton and methyl carbon) were assigned. Methylation analysis revealed terminal D-galactose 0.1 mol), 1,4-linked D-glucose (1.0 mol) and 1,2,3,4,6-linked D-galactose (3.7 mol) for native polysaccharide, and terminal D-galactose, 1,4-linked D-galactose (1.9 mol), 1,4-linked D-glucose (1.0 mol), 1,3- linked D-galactose (1.7 mol), and 1,3,4,6-linked D-galactose (0.3 mol) which substituted with pyruvate group at 4 and 6 positions for desulfated polysaccharide. The polysaccharide was the novel pyruvated glucogalactan sulfate, the structure of which was proposed.
基金Supported by the Faculty of Marine Science and Fisheries Red Sea University(Grant No.FMSF\UG\2).
文摘Objective:To investigate some selected metabolic constituents of Hypnea valentiae(Turner)Montagne collected from the Red Sea coast of Sudan to evaluate the economic potential of the alga.Methods:Healthy thalli of the alga under investigation were collected,thoroughly cleaned,air dried and milled.Authentic analytical methods were used to determine moisture,ash,protein lipid and carrageenan contents.Fatty acids profile was revealed with gas chromatography instrumentation.Testing of phytochemical groups of compounds was based on the development of colouration and precipitation upon addition of certain chemical reagents to the extracts.Results:The ash content of the alga[(40.3±0.2)%of dry weight]was comparatively the highest among the parameters tested.The protein,lipid,and carrageenan constituted(9.30±0.70)%,(6.50±0.34)%,and(33.70±0.01)%,respectively of the alga dry matter.Hypnea valentiae(Turner)Montagne from Sudan Red Sea coast composed of 12 fatty acids,8 of which were unsaturated fatty acids and 4 were saturated fatty acids.The major unsaturated fatty acids in the alga was the trans-isomer of linoleic acid and linolelaidic acid(36.32%)followed by palmitoleic acid(13.64%).The major saturated fatty acids was the heptadecanoic acid amounting to 19.10%of the total fatty acids.Phytochemically the alga contained alkaloids,flavonoids and tannins.Conclusions:This alga may represent a promising source of functional food and therapeutic metabolites.Further investigation and critical evaluation of the bioactivity of the phytochemical compounds is required to assure the therapeutic potential of this alga.
文摘Two compositions,a pure ceramide and a sterol mixture,were isolated from the ethylacetate phase of ethanol extract of the Hypnea Charoides Lamx,collected from the South China Sea.The ceramide was identified by spectroscopic analysis as N-(2’hydroxytetracosyl)-1,3,4-trihydroxy-2-am ino-octadecane;The six sterols were detected by gas chromatography/mass spectrometry,and their structures were confirmed by spectroscopic analysis as Ergost-5,22-dien-3-ol,Ergost-5-en-3-ol,Stigmasta-5,22-dien-3-ol,Cholesta-5,22-dien-3-ol,Cholesta-5-en-3-ol,Stigmasta-5-en-3-ol.
