The interaction of the anticancer drug hypocrellin B (HB) or the mono-cysteine substituted hypocrellin B (MCHB) and calf thymus deoxyribonucleic acid (CT-DNA) has been investigated using spectral methods. The results ...The interaction of the anticancer drug hypocrellin B (HB) or the mono-cysteine substituted hypocrellin B (MCHB) and calf thymus deoxyribonucleic acid (CT-DNA) has been investigated using spectral methods. The results of UV-visible spectra show that the HB and MCHB could intercalate into the base-stacking domain of the CT-DNA double helix. The studies of fluorescence spectra and circular dichroism(CD) spectra also support the interacalation mechanism.展开更多
OBJECTIVE To prepare hypocrellin B-loaded apoferritin nanoparticles(HB-AFT NPs)for photodynamic therapy on tumor.METHODS HB-AFT NPs were prepared by taking advantage of the reversibleunfolding and refolding character ...OBJECTIVE To prepare hypocrellin B-loaded apoferritin nanoparticles(HB-AFT NPs)for photodynamic therapy on tumor.METHODS HB-AFT NPs were prepared by taking advantage of the reversibleunfolding and refolding character of apoferritin in different pH environments.The photophysical and photobiological properties of hypocrellin B-loaded apoferritin were measured.RESULETS HB molecules were successfully encapsulated within apoferritincavity.HB-AFT-NPs exhibited higher ROS productionthan free HB.Additionally,phototoxicity of HB-AFT NPs to MDA-MB-231 cells was significantly improved as compared to free HB.CONCLUSION Together these results demonstrate that hypocrellin B-loaded apoferritin might be a potential nanoscale photosensitizer.展开更多
OBJECTIVE To investigate the killing effect of the combined treatment of ultrasound and hypocrellin B,an active component isolated from a natural product Hypocrella bambuase,on Candida albicans.METHODS At first,Candid...OBJECTIVE To investigate the killing effect of the combined treatment of ultrasound and hypocrellin B,an active component isolated from a natural product Hypocrella bambuase,on Candida albicans.METHODS At first,Candida albicans are incubated with hypcrellin B and the uptake of hypocrellin B in Candida albicans was determined using fluorescence intensity analysis.And then the sensitized Candida albicans were exposed to low-intensity of ultrasound.After the combined treatment of ultrasound and hypocellin B,the growth of Candida albicans was evaluated using the colony counting method,and membrane integrity of Candida albicans was also analyzed by a flow cytometry with propidium iodide staining.RESULTS Hypocrellin B was fast absorbed by candida albicans.The growth of candida albicans was more significantly inhibited by the combined treatment of ultrasound and hypocrellin B than the controls(P<0.05).The more marked damages to membrane integrity were found in the combined treatment than the controls(P<0.05).CONCLUSION The combined treatment of ultrasound and hypocrellin B had significant killing effect on Candida albicans,indicating sonodynamic therapy with hypocrellin B from natural product might be a potential approach for combating fungal infections.展开更多
OBJECTIVE To investigate the effect of sonodynamic action on biofilm-producing Enterococcus faecalis using hypocrellin B from a natural product Hypocrella bambuase as a sensitizer.METHODS The uptake of hypocrellin B i...OBJECTIVE To investigate the effect of sonodynamic action on biofilm-producing Enterococcus faecalis using hypocrellin B from a natural product Hypocrella bambuase as a sensitizer.METHODS The uptake of hypocrellin B in biofilm-producing Enterococcus faecalis was measured using the fluorescent analysis method.After the combined treatment of hypocrellin B and ultrasound,the growth of biofilm-producing Enterococcus faecalis was measured using the colony counting method,the membrane integrity was detected using a flow cytometric analysis with propidium iodide staining.Chromosomal DNA of bacteria was analysed using apulsed-field gel electrophoresis(PFGE).RESULTS The uptake of hypocrellin B in biofilm-producing Enterocuccus faecalis reached a high peak at the 20 min incubation.After the combined treatment of hypocrellin B,the growth of biofilm-producing Enterocuccus faecalis significantly descreased and membrane integrity was remarkably damaged.However,no remarkable change in bacterial DNA was found.CONCLUSION Sonodynamic action of hypocrellin B had significant killing activity on Enterocuccus faecalis.展开更多
文摘The interaction of the anticancer drug hypocrellin B (HB) or the mono-cysteine substituted hypocrellin B (MCHB) and calf thymus deoxyribonucleic acid (CT-DNA) has been investigated using spectral methods. The results of UV-visible spectra show that the HB and MCHB could intercalate into the base-stacking domain of the CT-DNA double helix. The studies of fluorescence spectra and circular dichroism(CD) spectra also support the interacalation mechanism.
基金The project supported by the general grant fund from Hong Kong Research Grant Committee(476912)Health and Medical Research Fund(13120442)Innovation and Technology Fund of Shenzhen(CXZZ20120619150627260)
文摘OBJECTIVE To prepare hypocrellin B-loaded apoferritin nanoparticles(HB-AFT NPs)for photodynamic therapy on tumor.METHODS HB-AFT NPs were prepared by taking advantage of the reversibleunfolding and refolding character of apoferritin in different pH environments.The photophysical and photobiological properties of hypocrellin B-loaded apoferritin were measured.RESULETS HB molecules were successfully encapsulated within apoferritincavity.HB-AFT-NPs exhibited higher ROS productionthan free HB.Additionally,phototoxicity of HB-AFT NPs to MDA-MB-231 cells was significantly improved as compared to free HB.CONCLUSION Together these results demonstrate that hypocrellin B-loaded apoferritin might be a potential nanoscale photosensitizer.
基金The project supported by Health and Medical Research Fund(13120442)the General Research Fund(GRF)Grant from Hong Kong Research Grant Committee(476912)
文摘OBJECTIVE To investigate the killing effect of the combined treatment of ultrasound and hypocrellin B,an active component isolated from a natural product Hypocrella bambuase,on Candida albicans.METHODS At first,Candida albicans are incubated with hypcrellin B and the uptake of hypocrellin B in Candida albicans was determined using fluorescence intensity analysis.And then the sensitized Candida albicans were exposed to low-intensity of ultrasound.After the combined treatment of ultrasound and hypocellin B,the growth of Candida albicans was evaluated using the colony counting method,and membrane integrity of Candida albicans was also analyzed by a flow cytometry with propidium iodide staining.RESULTS Hypocrellin B was fast absorbed by candida albicans.The growth of candida albicans was more significantly inhibited by the combined treatment of ultrasound and hypocrellin B than the controls(P<0.05).The more marked damages to membrane integrity were found in the combined treatment than the controls(P<0.05).CONCLUSION The combined treatment of ultrasound and hypocrellin B had significant killing effect on Candida albicans,indicating sonodynamic therapy with hypocrellin B from natural product might be a potential approach for combating fungal infections.
基金The project supported by the General Research Fund Grant from Hong Kong Research Grant Committee(476912)Health and Medical Research Fund(13120442)
文摘OBJECTIVE To investigate the effect of sonodynamic action on biofilm-producing Enterococcus faecalis using hypocrellin B from a natural product Hypocrella bambuase as a sensitizer.METHODS The uptake of hypocrellin B in biofilm-producing Enterococcus faecalis was measured using the fluorescent analysis method.After the combined treatment of hypocrellin B and ultrasound,the growth of biofilm-producing Enterococcus faecalis was measured using the colony counting method,the membrane integrity was detected using a flow cytometric analysis with propidium iodide staining.Chromosomal DNA of bacteria was analysed using apulsed-field gel electrophoresis(PFGE).RESULTS The uptake of hypocrellin B in biofilm-producing Enterocuccus faecalis reached a high peak at the 20 min incubation.After the combined treatment of hypocrellin B,the growth of biofilm-producing Enterocuccus faecalis significantly descreased and membrane integrity was remarkably damaged.However,no remarkable change in bacterial DNA was found.CONCLUSION Sonodynamic action of hypocrellin B had significant killing activity on Enterocuccus faecalis.
