The course and severity of periodontitis can be significantly affected by bacterial virulence as well as host immunity dysfunction. Periodontal tissue destruction has been proved to result from cascade of cytokines sy...The course and severity of periodontitis can be significantly affected by bacterial virulence as well as host immunity dysfunction. Periodontal tissue destruction has been proved to result from cascade of cytokines synthesized by reactive cells upon stimulation by pathogenic bacteria and lipopolysaccharides within their cell membranes. The clinical use of genetically programmed cells, producing substances blocking IL-1, based on recombinant IL-1 antagonist, as well as cytokines activating fibroblasts and osteoblasts to regenerate the destroyed periodontal tissue could prove alternative to the conventional treatment. Another cytokine of interest in respect to periodontitis ethiopathogenesis is soluble tumor necrosis factor receptor I (sTNF RI). Observation of soluble TNF receptors as physiologic inhibitors of TNF led to its administration in therapeutic process as well as in therapy selected cases of aggressive periodontitis.展开更多
目的 :研究大鼠脑内白细胞介素 1I型受体 m RNA表达神经元的分布。材料和方法 :以 35S标记的 IL- 1Rt Ic DNA作为探针 ,采用原位杂交技术检测大鼠脑内 IL- 1Rt I m RNA表达神经元分布广泛而又不均匀。根据表达IL- 1Rt I m RNA神经元的...目的 :研究大鼠脑内白细胞介素 1I型受体 m RNA表达神经元的分布。材料和方法 :以 35S标记的 IL- 1Rt Ic DNA作为探针 ,采用原位杂交技术检测大鼠脑内 IL- 1Rt I m RNA表达神经元分布广泛而又不均匀。根据表达IL- 1Rt I m RNA神经元的密度变化 ,大鼠脑可分为四种密度区 :非常高、高、中等、低密度区。结论 :提示脑内白细胞介素 1通过其展开更多
目的:研究^(125)I粒子植入对免疫微环境的影响,以及^(125)I粒子植入联合抗程序性死亡受体-1(programmed cell death receptor-1,PD-1)治疗的抗肿瘤疗效。方法:在小鼠右后肢皮下注射Lewis肺癌(LLC)细胞构建肿瘤模型,利用流式细胞术分析^(...目的:研究^(125)I粒子植入对免疫微环境的影响,以及^(125)I粒子植入联合抗程序性死亡受体-1(programmed cell death receptor-1,PD-1)治疗的抗肿瘤疗效。方法:在小鼠右后肢皮下注射Lewis肺癌(LLC)细胞构建肿瘤模型,利用流式细胞术分析^(125)I粒子植入后PD-1、程序性死亡配体1(programmed death-ligand 1,PD-L1)、Treg细胞的表达。在小鼠右后肢(原位肿瘤)和左前肢(远位肿瘤)皮下注射LLC细胞,将小鼠随机分为PBS组、抗PD-1组、^(125)I粒子植入组和联合治疗组。绘制肿瘤生长曲线,流式分析肿瘤浸润CD4^(+)及CD8^(+)T细胞比例。结果:^(125)I粒子植入12天后PD-L1及PD-1表达上调(P<0.01),Treg表达无显著性差异(P=0.196)。与其余各组相比,联合治疗组小鼠原位和远位肿瘤生长均受到明显抑制(均P<0.05),肿瘤浸润CD8^(+)T细胞比例显著增加(均P<0.05)。结论:^(125)I粒子植入联合抗PD-1治疗能激活机体抗肿瘤免疫,协同抑制小鼠LLC生长。展开更多
文摘The course and severity of periodontitis can be significantly affected by bacterial virulence as well as host immunity dysfunction. Periodontal tissue destruction has been proved to result from cascade of cytokines synthesized by reactive cells upon stimulation by pathogenic bacteria and lipopolysaccharides within their cell membranes. The clinical use of genetically programmed cells, producing substances blocking IL-1, based on recombinant IL-1 antagonist, as well as cytokines activating fibroblasts and osteoblasts to regenerate the destroyed periodontal tissue could prove alternative to the conventional treatment. Another cytokine of interest in respect to periodontitis ethiopathogenesis is soluble tumor necrosis factor receptor I (sTNF RI). Observation of soluble TNF receptors as physiologic inhibitors of TNF led to its administration in therapeutic process as well as in therapy selected cases of aggressive periodontitis.
文摘目的 :研究大鼠脑内白细胞介素 1I型受体 m RNA表达神经元的分布。材料和方法 :以 35S标记的 IL- 1Rt Ic DNA作为探针 ,采用原位杂交技术检测大鼠脑内 IL- 1Rt I m RNA表达神经元分布广泛而又不均匀。根据表达IL- 1Rt I m RNA神经元的密度变化 ,大鼠脑可分为四种密度区 :非常高、高、中等、低密度区。结论 :提示脑内白细胞介素 1通过其
