Molecular Characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China

Objective To determine the molecular characterization of full-length genome of Japanese encephalitis virus （JEV） genotype V. Methods The full-length nucleotide sequences of JEV strains isolated from different locations and sources were used in sequence and phylogenetic analysis. Results The full-length genome of genotypes V JEV, XZ0934, and Muar strain were composed of 10 983 and 10 988 nucleotides respectively and shared a lower level of identity with JEV genotypes I-IV, ranging from 78.4% （G I, KV1899） to 79.7% （G III, JaGAr01）, for the nucleotide sequences, and from 90.0%（G I, KV1899） to 91.8%（G III, JaGAr01） for the amino acid sequences. The open reading frame （ORF） of JEV genotype V spanned nucleotides 96 to 10 397 and encoded 3 433 amino acids. Interestingly, a comparison with JEV genotype I-IV revealed that 3 nucleotides （encoded with a serine residue） were inserted in the NS4A gene of JEV genotype V, and the insertion of nucleotides was also found in downstream of the ORF stop codon in 3’-untranslated region. Moreover, numerous amino acid mutations were observed in 3 functional domains of the E gene of JEV genotype V. Conclusion The molecular characterization of JEV genotype V is significantly different from that of the known genotypes I-IV. The mutations located in the coding region and the non-coding region may be molecular markers of JEV genotype V and warrant further studies to determine their effects on biology and immunogenicity of genotype V strains.

Antimicrobial Resistance,Virulence Profile,and Molecular Characterization of Listeria monocytogenes Isolated from Ready-to-eat Food in China,2013-2014

We aimed to investigate the potential pathogenic profile and antibiotic resistance of Listeria monocytogenes isolated from ready-to-eat food in China.Antimicrobial resistance was determined by broth microdilution following the Clinical and Laboratory Standards Institute protocol Molecular serotyping,virulence,and resistance genes were identified using PCR.Multi-locus

Preparation and Characterization of Single-Phase Perovskite La_(0.6)Sr_(0.4)Co_(0.8)Fe_(0.2)O_(3-δ)

Single-phase perovskite La0.6Sr0.4Co0.8Fe0.2O3-δ has been successfully prepared by using citrate-EDTA complexation method at relatively low calcination temperature. The structure and thermal decomposition process of the complex precursor have been investigated by means of differential scanning calorimetry-thermal gravimetric analysis （DSC/TGA）, X-ray diffraction （XRD）, and Fourier transform infrared spectroscopic （FT-IR） measurements. The precursor decomposed completely and started to form perovskite-type oxide above 420 ℃ according to the differential scanning calorimetry （DSC） and thermal gravimetric analysis （TGA） results. Single-phase perovskite La0.6Sr0.4Co0.8Fe0.2O3-δ obtained has been confirmed from the XRD pattern, and no peak of SrCO3 was found by XRD of the oxides synthesized at a relatively low temperature of 800℃. The reducibility of La0.6Sr0.4Co0.8Fe0.2O3-δ was also characterized by the temperature programmed reduction （TPR） technique. Disk shaped dense La0.6Sr0.4Co0.8Fe0.2O3-δ membrane was prepared by the isostatical pressing method. The oxygen flux rate of dense La0.6Sr0.4Co0.8Fe0.2O3-δ membrane was （2.8-18）× 10^-8 mol/（cm^2.s） in the temperature range of 800-1000 ℃.

Characterization and Modelling of High Temperature Flow Behaviour of V Modified 2.25Cr-1Mo Heat Resistant Steel Plate

In order to study the high temperature flow behaviour of the V modified 2.25Cr-1Mo steel plate to guide the industrial rolling practice, the hot compression tests were carried out at the temperatures from 900 ℃ to 1150 ℃ and the strain rates from 0.01s^-1 to 1 s^-1 on Thermecmastor-Z equipment. Based on the experimental data of the hot compression tests, a kind of Arrhenius-type constitutive equation was developed.The equation can accurately show the relationship between the flow stress and the deformation temperature, the strain and the strain rate. The measured true stress-true strain curves exhibit two kinds of flow stress curves. Moreover, the forming mechanisms of these two types curves were explained by softening, wok hardening theory as well as metallographic and hardness experimental results. The accuracy of the developed Arrheniustype constitutive equation was identified by three kinds of statistic parameters and also by comparison of the measured and predicted data. The reasonable value of the three types of statistic parameters and the good agreement between the experimental and predicted data can confirm the validity of the developed Arrheniustype constitutive equation for V modified 2.25 Cr-1 Mo heat resistant steel plate.

Fabrication and Characterization of 1700 V 4H-SiC Vertical Double-Implanted Metal-Oxide-Semiconductor Field-Effect Transistors

The fabrication and characterization of 1700 V 7 A 4H-SiC vertical double-implanted metal-oxide-semiconductor field-effect transistors （VDMOSFETs） are reported. The drift layer is 17μm in thickness with 5 × 10^15 cm^-3 n-type doping, and the channel length is 1μm. The MOSFETs show a peak mobility of 17cm2/V.s and a typical threshold voltage of 3 V. The active area of 0.028cm2 delivers a forward drain current of 7A at Vcs = 22 V and VDS= 15 V. The specific on-resistance （Ron,sv） is 18mΩ.cm2 at VGS= 22 V and the blocking voltage is 1975 V （IDS 〈 lOOnA） at VGS = 0 V.

Characterization and Expression Analysis of Peroxiredoxin Genes in NNK-induced V79 Cells

4-（Methylnitrosamino）-1-（3-pyridyl）-1-butanone （NNK） is a potent and prevalent nitrosamine procarcinogen found in cigarette smoke. The aim of this work is to study alterations in peroxiredoxin （Prx） expression induced by NNK during carcinogenesis. Characterization of Prx genes from hamster was performed using bioinformatics.

SYNTHESIS AND CHARACTERIZATION OF A SERIES OF NOVEL INORGANIC MICROPOROUS CRYSTALS,BePO_4-CIn(n=4-7).

A series of novet beryllophosphate zeolites,named BePO_4-CIn(n=4-7), are synthesized hydrothermally and characterized with X-ray powder diffraction,IR spectra,SEM,thermat analysis and ion-exchange.

V2V Channel Characterization and Modeling for Underground Parking Garages

As an important part of the intelligent transportation system(ITS),vehicle-to-vehicle(V2V)communication will improve the safety and efficiency of the transportation system by realizing the information transmission between vehicles.The underground parking garage is a typical scenario in V2V communication,but as an indoor environment,it is different from the conventional outdoor road scenarios significantly.Therefore,the purpose of this paper is to analyze and model the V2V channel characteristics of an underground garage.In this paper,a novel channel measurement carried out in the underground garage is first introduced.Considering that there are a lot of obstacles in an underground garage,the non-line-of-sight(NLoS)condition is taken into account during the measurement.Then,channel characteristic parameters,including large-scale fading,delay spread,and K-factor,are analyzed based on the measured data.In addition,we also carry out channel angle of arrival analysis,show the distribution of azimuth of arrival(AOA)and elevation of arrival(EOA)of multipath components(MPC),and conduct a statistical analysis of the angular spread.In the process of analysis and modeling,we focus on the differences between line-of-sight(LoS)and NLoS conditions.The analysis and conclusions presented in this paper will enrich the understanding of V2V channel and benefit the design of V2V communications.

Synthesis and Structural Characterization of 2,3-Bis(hydroxymethyl)-2,3-dinitro-1,4-butanediol

2,3-Bis（hydroxymethyl）-2,3-dinitro-1,4-butanediol（C6H12N2O8） was synthesized by condensation,cyclization,oxidative dimerization and deketalization of nitromethane with a total yield of 42.4%.The structure of the title compound was characterized by 1H NMR,13C NMR,FT-IR,elementary analysis,and X-ray single-crystal diffraction analysis,which reveals that the title compound crystallizes in triclinic,space group P with a = 0.6324（2）,b = 0.6454（3）,c = 0.7062（3） nm,α= 111.550（4）,β= 95.505（4）,γ= 113.395（4）°,V = 0.23595（16） nm3,Z = 1,Mr = 240.18,Dc = 1.690 g·cm-3,μ = 0.159 mm-1,F（000） = 126,R = 0.0304 and wR = 0.0907.

Synthesis,Crystal Structure and Characterization of a New Coordination Polymer from the Self-assembly of CoCl_2 Salt and Flexible Ligand 1,3-Bis(4-pyridyl)propane

A new 1D coordination polymer [Co（bpp）3Cl2（H2O）2]n 1 （bpp = 1,3-bis（4-pyridyl）-propane） was synthesized and characterized by elemental analysis,IR spectrum and single-crystal X-ray diffraction. The crystal belongs to the orthorhombic system,space group Ibca with a = 16.569（9）,b = 17.240（10）,c = 27.087（16） ,V = 7738（8） 3,Z = 8,Dc = 1.306 g/cm3,Mr = 760.65,λ（MoKa） = 0.71073 ,μ = 0.623 mm1,F（000） = 3192,the final R = 0.0678 and wR = 0.2011. The Co（II） atom is coordinated in a slightly distorted octahedral CoN4Cl2 geometry by two Cl atoms in the axial positions,four N atoms from the two bridging bpp ligands and two pendant bpp ligands. The CoN4Cl2 octahedra are connected by the bridging bpp ligands to form a 1D neutral coordination polymer chain. The chains are linked by face-to-face π-π interactions between adjacent pendant bpp ligands to give rise to a 3D supramolecular architecture. The photoluminescent investigation indicates that the emission of 1 is attributed to ligand-centered emission. The variable-temperature magnetic susceptibility measurement shows weak antiferromagnetic behavior in the coπmplex.

固溶与固溶时效热处理对Ti-6Al-4V合金微观组织及力学性能的影响

通过对Ti-6A1-4V钛合金螺栓紧固件的热处理实验,比较分析了固溶及固溶时效热处理对Ti-6A1-4V紧固件的微观组织分布的影响,并通过显微硬度计得到了螺栓紧固件各个位置的显微硬度分布。实验结果表明:Ti-6A1-4V螺栓经过固溶处理后,初生α组织的数量与尺寸减少而针状α组织的数量增多,并且初生α组织出现了即将溶解的状态。经过固溶时效热处理后,等轴状的初生α相组织变成了大晶粒β相的片层组织,组织分布较为均匀。固溶时效件试样的硬度分布更为均匀,固溶时效可以有效地提高螺栓的硬度,从而达到材料的使用要求。

Ti-6Al-4V ELI钛合金的宽温域(-253~350℃)力学性能研究

钛及钛合金是一种重要的太空金属材料,在航空航天领域发挥重要的作用。研究了Ti-6Al-4V ELI钛合金在室温(20℃)、高温(350℃)以及超低温(-253℃)环境下的拉伸力学性能,比较了Ti-6Al-4V ELI钛合金在不同温度下的力学性能指标,分析了其断口形貌特征。结果表明,Ti-6Al-4V ELI钛合金在超低温下表现出较高的力学性能,抗拉强度可达1900 MPa以上,其断口形貌呈现脆性断裂特点。在室温和高温下,Ti-6Al-4V ELI钛合金表现出良好的塑性和韧性,具有较为明显的韧性断裂特点。因其良好的宽温域力学性能,Ti-6Al-4V ELI钛合金是未来发展航天航空领域必不可少的重要金属材料。

具有多级适应性休假和Min(N,V)-策略的Geo=G=1排队系统分析

本文研究具有多级适应性休假和Min(N,V)-策略的Geo/G/1排队系统,运用更新过程理论,全概率分解技术和z-变换工具,分析在时刻点n^(+)的队长瞬态概率分布的z-变换表达式.此外,基于瞬态分析,使用洛必达法则得到系统在时刻点n+的队长稳态分布的递推表达式,然后给出稳态队长分布的概率母函数以及平均稳态队长的表达式.接着,探讨在不同时刻点n^(-)、n和外部观察点的稳态概率分布.最后,在建立费用结构模型下,由更新报酬过程理论获得系统长期运行单位时间内所产生的成本期望费用的表达式,并通过数值实例讨论最优控制策略N^(*)以及当连续休假次数为正整数J时的二维最优控制策略(N^(*),J^(*)).

基于S7-300PLC的净水厂V型滤池控制系统设计思路探究

净水厂是城市供水的主要来源,净水厂出厂水质的安全可靠是确保安全饮用水的首要任务。滤池是净水厂工艺流程中水处理关键环节,对出厂水质产生直接影响。随着科技的发展和进步,实现净水厂滤池自动化高效运行,本文围绕基于S7-300 PLC的净水厂V型滤池控制系统设计展开一系列的探讨,分析研究了净水厂V型滤池在正常过滤过程和反冲洗功能的难点和解决方法,并实现净水厂V型滤池在正常过滤过程和反冲洗功能控制系统精确控制,经过三亚市某水厂的实际运行检验,系统可靠稳定,同时使得净水厂出厂水质符合生活饮用水标准,希望能给净水厂V型滤池控制系统设计上带来一些帮助。

SYNTHESIS AND ^（183）W，^（51）V NMR CHARACTERIZATION OF a－［GaW9V8O（40）］（5－） HETEROPOLYANION

The trivanadium-subatituted heteropolytungstates have been synthesized from their lacunary precusor a-Na9GaW9O(34)H2.(183)W NMR spectrum of the title anion consists of two lines as expected for the C3v structure of the trisubetituted A-type Keggin anion.

直流-48 V叠光供电系统在通信机楼的应用分析

2022年中国电信提出“1248”双碳行动计划,目的是减少企业碳排放量,推动“双碳”目标的实现,而在此过程中,绿电使用占比和增速提升是达到“双碳”目标的必然路径,且绿电替代化石能源也是未来主要的发展趋势。现阶段绿电主要包括风电和光伏发电,获得绿电的途径主要有绿电交易和分布式光伏发电自发自用。目前光伏发电在各类通信局站已广泛应用,结合通信局站供电系统自身特点,对一种更加灵活高效的直流叠光供电系统进行了介绍,重点阐述其系统组成、结构及特点,并结合典型应用场景对经济效益及节能减排效果进行了分析。

Current-voltage characteristics of an individual helical CdS nanowire rope

This paper studies the electronic transport in an individual helically twisted CdS nanowire rope, on which platinum microleads are attached by focused-ion beam deposition. The current-voltage （I - V） characteristics are nonlinear from 300 down to 60 K. Some step-like structures in the I - V curves and oscillation peaks in the differential conductance （dI/dV - V） curves have been observed even at room temperature. It proposes that the observed behaviour can be attributed to Coulomb-blockade transport in the one-dimensional CdS nanowires with diameters of 6-10 nm.

新型磷酸钒孔道结构化合物(H_3NCH_2CH_2NH_3)_3-[(VO)_4(PO_4)_2(HPO_4)_4]的水热合成及结构表征

以有机分子乙二胺作为模板剂合成了新型磷酸钒孔道化合物 (H3NCH2 CH2 NH3) 3[(VO) 4(PO4 ) 2 (HPO4 ) 4],并通过X射线单晶衍射实验进行了结构表征 .晶体学数据为 :C2 /c ,a =1.85 0 5 ( 9)nm ,b =0 .70 89( 4)nm ,c=2 .3 3 0 4( 10 )nm ,β =96.43 ( 3 )°,V =3 .0 3 8( 3 )nm3,Z =8,R =0 .0 67,Rbw=0 .163 5 .该化合物具有非常独特和规整的二维孔道骨架结构 。

激光快速修复Ti-6Al-4V合金的显微组织与力学性能

针对Ti-6Al-4V合金在加工和服役过程中的损伤特点,对Ti-6Al-4V合金锻件的3种典型误加工缺陷——槽缺陷、面缺陷和体缺陷进行了激光快速修复研究。激光修复区与锻件基体形成致密冶金结合,Al、V合金元素由锻件基体到激光修复区均匀分布,无宏观偏析。激光修复区组织为粗大原始β晶粒内分布细长的α针及编织细密的α+β板条组织,呈现典型的魏氏结构,热影响区组织从锻件的等轴α+转变β组织逐步过渡到魏氏(α+β)组织。对预制有3种类型缺陷的激光修复试样进行室温静载拉伸试验和硬度测试,结果表明修复试样的拉伸性能达到锻件标准(HB5224-1982)。激光修复试样的硬度和强度高于锻件基体,而塑性则低于锻件基体。因此,激光修复区和锻件基体可看作是一种“强+弱”的组合,这与二者的显微组织是相对应的。

基于MATLAB平台对V5-2000大地电磁测深仪TBL文件的分析与应用

在大地电磁法勘探中,为了快速从工区项目众多文件中选出与某一特定点位具有重叠采集时间的点位文件,并进行远参考处理或便捷地读取一些实测点位信息,常用的方法是利用SSMT2000处理软件,找出具有重叠采集时间的点位文件和从处理输出结果EDI文件中,逐一读取相应参数。如果采用编程的思想直接从原始TBL参数文件中读取相应参数,便可快速达到此目的。鉴于MATLAB语言最近在地学领域内越来越被广泛使用,这里利用MATLAB平台对V5—2000大地电磁测深仪TBL参数文件进行了分析,并给出相应读取程序,在提取所需信息后可以快速提高此类问题的工作效率。