Nanomaterial-assisted wearable glucose biosensors for noninvasive real-time monitoring:Pioneering point-of-care and beyond

This review explores glucose monitoring and management strategies,emphasizing the need for reliable and userfriendly wearable sensors that are the next generation of sensors for continuous glucose detection.In addition,examines key strategies for designing glucose sensors that are multi-functional,reliable,and cost-effective in a variety of contexts.The unique features of effective diabetes management technology are highlighted,with a focus on using nano/biosensor devices that can quickly and accurately detect glucose levels in the blood,improving patient treatment and control of potential diabetes-related infections.The potential of next-generation wearable and touch-sensitive nano biomedical sensor engineering designs for providing full control in assessing implantable,continuous glucose monitoring is also explored.The challenges of standardizing drug or insulin delivery doses,low-cost,real-time detection of increased blood sugar levels in diabetics,and early digital health awareness controls for the adverse effects of injectable medication are identified as unmet needs.Also,the market for biosensors is expected to expand significantly due to the rising need for portable diagnostic equipment and an ever-increasing diabetic population.The paper concludes by emphasizing the need for further research and development of glucose biosensors to meet the stringent requirements for sensitivity and specificity imposed by clinical diagnostics while being cost-effective,stable,and durable.

Real-Time Monitoring Method for Cow Rumination Behavior Based on Edge Computing and Improved MobileNet v3

[Objective]Real-time monitoring of cow ruminant behavior is of paramount importance for promptly obtaining relevant information about cow health and predicting cow diseases.Currently,various strategies have been proposed for monitoring cow ruminant behavior,including video surveillance,sound recognition,and sensor monitoring methods.How‐ever,the application of edge device gives rise to the issue of inadequate real-time performance.To reduce the volume of data transmission and cloud computing workload while achieving real-time monitoring of dairy cow rumination behavior,a real-time monitoring method was proposed for cow ruminant behavior based on edge computing.[Methods]Autono‐mously designed edge devices were utilized to collect and process six-axis acceleration signals from cows in real-time.Based on these six-axis data,two distinct strategies,federated edge intelligence and split edge intelligence,were investigat‐ed for the real-time recognition of cow ruminant behavior.Focused on the real-time recognition method for cow ruminant behavior leveraging federated edge intelligence,the CA-MobileNet v3 network was proposed by enhancing the MobileNet v3 network with a collaborative attention mechanism.Additionally,a federated edge intelligence model was designed uti‐lizing the CA-MobileNet v3 network and the FedAvg federated aggregation algorithm.In the study on split edge intelli‐gence,a split edge intelligence model named MobileNet-LSTM was designed by integrating the MobileNet v3 network with a fusion collaborative attention mechanism and the Bi-LSTM network.[Results and Discussions]Through compara‐tive experiments with MobileNet v3 and MobileNet-LSTM,the federated edge intelligence model based on CA-Mo‐bileNet v3 achieved an average Precision rate,Recall rate,F1-Score,Specificity,and Accuracy of 97.1%,97.9%,97.5%,98.3%,and 98.2%,respectively,yielding the best recognition performance.[Conclusions]It is provided a real-time and effective method for monitoring cow ruminant behavior,and the proposed federated edge intelligence model can be ap‐plied in practical settings.

Real-Time Monitoring of Meteorological Data at In-Situ GCW Remediation Sites

To optimize the self-organization network, self-adaptation, real-time monitoring, remote management capability, and equipment reuse level of the meteorological station supporting the portable groundwater circulation wells, and to provide real-time and effective technical services and environmental data support for groundwater remediation, a real-time monitoring system design of the meteorological station supporting the portable groundwater circulation wells based on the existing equipment is proposed. A variety of environmental element information is collected and transmitted to the embedded web server by the intelligent weather transmitter, and then processed by the algorithm and stored internally, displayed locally, and published on the web. The system monitoring algorithm and user interface are designed in the CNWSCADA development environment to realize real-time processing and analysis of environmental data and monitoring, control, management, and maintenance of the system status. The PLC-controlled photovoltaic power generating panels and lithium battery packs are in line with the concept of energy saving and emission reduction, and at the same time, as an emergency power supply to guarantee the safety of equipment and data when the utility power fails to meet the requirements. The experiment proves that the system has the characteristics of remote control, real-time interaction, simple station deployment, reliable operation, convenient maintenance, and green environment protection, which is conducive to improving the comprehensive utilization efficiency of various types of environmental information and providing reliable data support, theoretical basis and guidance suggestions for the research of groundwater remediation technology and its disciplines, and the research and development of the movable groundwater cycling well monitoring system.

Real-time prediction of mechanical behaviors of underwater shield tunnel structure using machine learning method based on structural health monitoring data

Predicting the mechanical behaviors of structure and perceiving the anomalies in advance are essential to ensuring the safe operation of infrastructures in the long run.In addition to the incomplete consideration of influencing factors,the prediction time scale of existing studies is rough.Therefore,this study focuses on the development of a real-time prediction model by coupling the spatio-temporal correlation with external load through autoencoder network(ATENet)based on structural health monitoring(SHM)data.An autoencoder mechanism is performed to acquire the high-level representation of raw monitoring data at different spatial positions,and the recurrent neural network is applied to understanding the temporal correlation from the time series.Then,the obtained temporal-spatial information is coupled with dynamic loads through a fully connected layer to predict structural performance in next 12 h.As a case study,the proposed model is formulated on the SHM data collected from a representative underwater shield tunnel.The robustness study is carried out to verify the reliability and the prediction capability of the proposed model.Finally,the ATENet model is compared with some typical models,and the results indicate that it has the best performance.ATENet model is of great value to predict the realtime evolution trend of tunnel structure.

Towards Cache-Assisted Hierarchical Detection for Real-Time Health Data Monitoring in IoHT

Real-time health data monitoring is pivotal for bolstering road services’safety,intelligence,and efficiency within the Internet of Health Things(IoHT)framework.Yet,delays in data retrieval can markedly hinder the efficacy of big data awareness detection systems.We advocate for a collaborative caching approach involving edge devices and cloud networks to combat this.This strategy is devised to streamline the data retrieval path,subsequently diminishing network strain.Crafting an adept cache processing scheme poses its own set of challenges,especially given the transient nature of monitoring data and the imperative for swift data transmission,intertwined with resource allocation tactics.This paper unveils a novel mobile healthcare solution that harnesses the power of our collaborative caching approach,facilitating nuanced health monitoring via edge devices.The system capitalizes on cloud computing for intricate health data analytics,especially in pinpointing health anomalies.Given the dynamic locational shifts and possible connection disruptions,we have architected a hierarchical detection system,particularly during crises.This system caches data efficiently and incorporates a detection utility to assess data freshness and potential lag in response times.Furthermore,we introduce the Cache-Assisted Real-Time Detection(CARD)model,crafted to optimize utility.Addressing the inherent complexity of the NP-hard CARD model,we have championed a greedy algorithm as a solution.Simulations reveal that our collaborative caching technique markedly elevates the Cache Hit Ratio(CHR)and data freshness,outshining its contemporaneous benchmark algorithms.The empirical results underscore the strength and efficiency of our innovative IoHT-based health monitoring solution.To encapsulate,this paper tackles the nuances of real-time health data monitoring in the IoHT landscape,presenting a joint edge-cloud caching strategy paired with a hierarchical detection system.Our methodology yields enhanced cache efficiency and data freshness.The corroborative numerical data accentuates the feasibility and relevance of our model,casting a beacon for the future trajectory of real-time health data monitoring systems.

Study on the Application of Real-Time Drone Monitoring in Ordos Open-Pit Coal Mine

In the process of intelligent mine construction in open-pit mine, in order to improve the safety monitoring ability of mine transportation system, solve the problems of large human interference and blind Angle detection by existing conventional monitoring methods, this paper establishes an open-pit mine monitoring data set, and proposes a real-time intelligent monitoring model based on UAV. The reasoning component with strong computing power and low power consumption is selected, and the lightweight object detection model is selected for the experiment. A quantitative standard of dynamic energy consumption detection by evaluation algorithm is proposed. Through experimental comparison, it is found that YOLOv4-tiny has the highest comprehensive grade in detection accuracy, speed, energy consumption and other aspects, which is suitable for application in the above model.

Feasibility Assessment of Fast Numerical Simulations for Real-Time Monitoring and Control of PEM Fuel Cells

Computational models that ensure accurate and fast responses to the variations in operating conditions,such as the cell tem-perature and relative humidity(RH),are essential monitoring tools for the real-time control of proton exchange membrane(PEM)fuel cells.To this end,fast cell-area-averaged numerical simulations are developed and verifi ed against the present experiments under various RH levels.The present simulations and measurements are found to agree well based on the cell voltage(polarization curve)and power density under variable RH conditions(RH=40%,RH=70%,and RH=100%),which verifi es the model accuracy in predicting PEM fuel cell performance.In addition,computationally feasible reduced-order models are found to deliver a fast output dataset to evaluate the charge/heat/mass transfer phenomena as well as water production and two-phase fl ow transport.Such fast and accurate evaluations of the overall fuel cell operation can be used to inform the real-time control systems that allow for the improved optimization of PEM fuel cell performance.

基于Real-time PCR法检测乳粉中牛源性成分定量研究

基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛源性成分的相对定量检测。结果显示,该方法的最低检测限为0.00001 mg/mL,回收率为91.11%~119.2%,组间变异系数≤0.58%、组内变异系数≤1.44%。说明该方法在特异性与稳定性上适用于乳粉中牛源性成分及含量的掺假检测。

Application and management of continuous glucose monitoring in diabetic kidney disease

Diabetic kidney disease(DKD)is a common complication of diabetes mellitus that contributes to the risk of end-stage kidney disease(ESKD).Wide glycemic var-iations,such as hypoglycemia and hyperglycemia,are broadly found in diabetic patients with DKD and especially ESKD,as a result of impaired renal metabolism.It is essential to monitor glycemia for effective management of DKD.Hemoglobin A1c(HbA1c)has long been considered as the gold standard for monitoring glycemia for>3 months.However,assessment of HbA1c has some bias as it is susceptible to factors such as anemia and liver or kidney dysfunction.Continuous glucose monitoring(CGM)has provided new insights on glycemic assessment and management.CGM directly measures glucose level in interstitial fluid,reports real-time or retrospective glucose concentration,and provides multiple glycemic metrics.It avoids the pitfalls of HbA1c in some contexts,and may serve as a precise alternative to estimation of mean glucose and glycemic variability.Emerging studies have demonstrated the merits of CGM for precise monitoring,which allows fine-tuning of glycemic management in diabetic patients.Therefore,CGM technology has the potential for better glycemic monitoring in DKD patients.More research is needed to explore its application and management in different stages of DKD,including hemodialysis,peritoneal dialysis and kidney transplantation.

Push forward LC-MS-based therapeutic drug monitoring and pharmacometabolomics for anti-tuberculosis precision dosing and comprehensive clinical management

The spread of tuberculosis(TB),especially multidrug-resistant TB and extensively drug-resistant TB,has strongly motivated the research and development of new anti-TB drugs.New strategies to facilitate drug combinations,including pharmacokinetics-guided dose optimization and toxicology studies of first-and second-line anti-TB drugs have also been introduced and recommended.Liquid chromatography-mass spectrometry(LC-MS)has arguably become the gold standard in the analysis of both endo-and exo-genous compounds.This technique has been applied successfully not only for therapeutic drug monitoring(TDM)but also for pharmacometabolomics analysis.TDM improves the effectiveness of treatment,reduces adverse drug reactions,and the likelihood of drug resistance development in TB patients by determining dosage regimens that produce concentrations within the therapeutic target window.Based on TDM,the dose would be optimized individually to achieve favorable outcomes.Pharmacometabolomics is essential in generating and validating hypotheses regarding the metabolism of anti-TB drugs,aiding in the discovery of potential biomarkers for TB diagnostics,treatment monitoring,and outcome evaluation.This article highlighted the current progresses in TDM of anti-TB drugs based on LC-MS bioassay in the last two decades.Besides,we discussed the advantages and disadvantages of this technique in practical use.The pressing need for non-invasive sampling approaches and stability studies of anti-TB drugs was highlighted.Lastly,we provided perspectives on the prospects of combining LC-MS-based TDM and pharmacometabolomics with other advanced strategies(pharmacometrics,drug and vaccine developments,machine learning/artificial intelligence,among others)to encapsulate in an all-inclusive approach to improve treatment outcomes of TB patients.

Fiber optic monitoring of an anti-slide pile in a retrogressive landslide

Anti-slide piles are one of the most important reinforcement structures against landslides,and evalu-ating the working conditions is of great significance for landslide mitigation.The widely adopted analytical methods of pile internal forces include cantilever beam method and elastic foundation beam method.However,due to many assumptions involved in calculation,the analytical models cannot be fully applicable to complex site situations,e.g.landslides with multi-sliding surfaces and pile-soil interface separation as discussed herein.In view of this,the combination of distributed fiber optic sensing(DFOS)and strain-internal force conversion methods was proposed to evaluate the working conditions of an anti-sliding pile in a typical retrogressive landslide in the Three Gorges reservoir area,China.Brillouin optical time domain reflectometry(BOTDR)was utilized to monitor the strain distri-bution along the pile.Next,by analyzing the relative deformation between the pile and its adjacent inclinometer,the pile-soil interface separation was profiled.Finally,the internal forces of the anti-slide pile were derived based on the strain-internal force conversion method.According to the ratio of calculated internal forces to the design values,the working conditions of the anti-slide pile could be evaluated.The results demonstrated that the proposed method could reveal the deformation pattern of the anti-slide pile system,and can quantitatively evaluate its working conditions.

Application of GNSS-PPP on Dynamic Deformation Monitoring of Offshore Platforms

The real-time dynamic deformation monitoring of offshore platforms under environmental excitation is crucial to their safe operation.Although Global Navigation Satellite System-Precise Point Positioning(GNSS-PPP)has been considered for this purpose,its monitoring accuracy is relatively low.Moreover,the influence of background noise on the dynamic monitoring accuracy of GNSS-PPP remains unclear.Hence,it is imperative to further validate the feasibility of GNSS-PPP for deformation monitoring of offshore platforms.To address these concerns,vibration table tests with different amplitudes and frequencies are conducted.The results demonstrate that GNSS-PPP can effectively monitor horizontal vibration displacement as low as±30 mm,which is consistent with GNSS-RTK.Furthermore,the spectral characteristic of background noise in GNSS-PPP is similar to that of GNSS-RTK(Real Time Kinematic).Building on this observation,an improved Complete Ensemble Empirical Mode Decomposition with Adaptive Noise(CEEMDAN)has been proposed to de-noise the data and enhance the dynamic monitoring accuracy of GNSS-PPP.Field monitoring application research is also undertaken,successfully extracting and analyzing the dynamic deformation of an offshore platform structure under environmental excitation using GNSS-PPP monitoring in conjunction with improved CEEMDAN de-noising.By comparing the de-noised dynamic deformation trajectories of the offshore platform during different periods,it is observed that the platform exhibits reversible alternating vibration responses under environmental excitation,with more pronounced displacement deformation in the direction of load action.The research results confirm the feasibility and potential of GNSS-PPP for dynamic deformation monitoring of offshore platforms.

一种基于real-time PCR技术的TTV检测方法的建立及应用

目的:本研究旨在开发一种具有更高灵敏度和特异性的TTV检测技术,为揭示TTV在多种疾病过程中的作用提供重要的技术支持。方法:为了更精确、灵敏的检测TTV,本研究分析了目前公布的所有亚型的TTV基因序列,在此基础上建立了一种基于UTR区域的real-time PCR检测方法,并与文献报道应用较为广泛的PCR检测方法进行了对比。结果:本研究建立的方法在1×10^(7)~1×10^(1) copies/μL标准品浓度范围内具有良好的线性关系,相关系数为1.000,斜率为-3.446,检测下限为1×10^(1) copies/μL。重复性试验结果显示,组内变异系数为7.22%,表明本方法重复性、稳定性较强。针对30份临床样本,使用本研究建立的real-time PCR检测方法及目前被多个研究所使用的4套引物进行对比。结果表明,本研究所建立的方法灵敏度显著高于文献中报道的4种方法(P<0.01);Sanger测序结果表明,本方法检测出的30份阳性样本均为TTV,检测特异性为100%。结论:本研究采用基于TaqMan探针的real-time PCR检测方法,检测灵敏性高、覆盖基因型范围广,尤其对于TTV病毒载量较低的情况下能够进行定量检测,对于TTV病毒的致病性及作为免疫标志物的应用提供重要的技术支持。

Monitoring seismicity in the southern Sichuan Basin using a machine learning workflow

Monitoring seismicity in real time provides significant benefits for timely earthquake warning and analyses.In this study,we propose an automatic workflow based on machine learning(ML)to monitor seismicity in the southern Sichuan Basin of China.This workflow includes coherent event detection,phase picking,and earthquake location using three-component data from a seismic network.By combining Phase Net,we develop an ML-based earthquake location model called Phase Loc,to conduct real-time monitoring of the local seismicity.The approach allows us to use synthetic samples covering the entire study area to train Phase Loc,addressing the problems of insufficient data samples,imbalanced data distribution,and unreliable labels when training with observed data.We apply the trained model to observed data recorded in the southern Sichuan Basin,China,between September 2018 and March 2019.The results show that the average differences in latitude,longitude,and depth are 5.7 km,6.1 km,and 2 km,respectively,compared to the reference catalog.Phase Loc combines all available phase information to make fast and reliable predictions,even if only a few phases are detected and picked.The proposed workflow may help real-time seismic monitoring in other regions as well.

Natural Disaster Risk Monitoring for Immovable Cultural Relics Based on Digital Twin

Natural disaster risk monitoring is an important task for disaster prevention and reduction.In the case of immovable cultural relics,however,the feedback mechanism,risk factors,monitoring logic,and monitoring indicators of natural disaster risk monitoring are complex.How to achieve intelligent perception and monitoring of natural disaster risk for immovable cultural relics has always been a focus and a challenge for researchers.Based on the analysis of the concepts and issues related to the natural disaster risk of immovable cultural relics,this paper proposes a framework for natural disaster risk monitoring for immovable cultural relics based on the digital twin.This framework focuses on risk monitoring,including the physical entities of natural disaster risk for immovable cultural relics,monitoring indicators,and virtual entity construction.A platform for monitoring the natural disaster risk of immovable cultural relics is proposed.Using the Puzhou Ancient City Site as a test bed,the proposed concept can be used for monitoring the natural disaster risk of immovable cultural relics at different scales.

Interplay of laser power and pore characteristics in selective laser melting of ZK60 magnesium alloys:A study based on in-situ monitoring and image analysis

This study offers significant insights into the multi-physics phenomena of the SLM process and the subsequent porosity characteristics of ZK60 Magnesium(Mg)alloys.High-speed in-situ monitoring was employed to visualise process signals in real-time,elucidating the dynamics of melt pools and vapour plumes under varying laser power conditions specifically between 40 W and 60 W.Detailed morphological analysis was performed using Scanning-Electron Microscopy(SEM),demonstrating a critical correlation between laser power and pore formation.Lower laser power led to increased pore coverage,whereas a denser structure was observed at higher laser power.This laser power influence on porosity was further confirmed via Optical Microscopy(OM)conducted on both top and cross-sectional surfaces of the samples.An increase in laser power resulted in a decrease in pore coverage and pore size,potentially leading to a denser printed part of Mg alloy.X-ray Computed Tomography(XCT)augmented these findings by providing a 3D volumetric representation of the sample internal structure,revealing an inverse relationship between laser power and overall pore volume.Lower laser power appeared to favour the formation of interconnected pores,while a reduction in interconnected pores and an increase in isolated pores were observed at higher power.The interplay between melt pool size,vapour plume effects,and laser power was found to significantly influence the resulting porosity,indicating a need for effective management of these factors to optimise the SLM process of Mg alloys.

Pore-pressure and stress-coupled creep behavior in deep coal:Insights from real-time NMR analysis

Understanding the variations in microscopic pore-fracture structures(MPFS) during coal creep under pore pressure and stress coupling is crucial for coal mining and effective gas treatment. In this manuscript, a triaxial creep test on deep coal at various pore pressures using a test system that combines in-situ mechanical loading with real-time nuclear magnetic resonance(NMR) detection was conducted.Full-scale quantitative characterization, online real-time detection, and visualization of MPFS during coal creep influenced by pore pressure and stress coupling were performed using NMR and NMR imaging(NMRI) techniques. The results revealed that seepage pores and microfractures(SPM) undergo the most significant changes during coal creep, with creep failure gradually expanding from dense primary pore fractures. Pore pressure presence promotes MPFS development primarily by inhibiting SPM compression and encouraging adsorption pores(AP) to evolve into SPM. Coal enters the accelerated creep stage earlier at lower stress levels, resulting in more pronounced creep deformation. The connection between the micro and macro values was established, demonstrating that increased porosity at different pore pressures leads to a negative exponential decay of the viscosity coefficient. The Newton dashpot in the ideal viscoplastic body and the Burgers model was improved using NMR experimental results, and a creep model that considers pore pressure and stress coupling using variable-order fractional operators was developed. The model’s reasonableness was confirmed using creep experimental data. The damagestate adjustment factors ω and β were identified through a parameter sensitivity analysis to characterize the effect of pore pressure and stress coupling on the creep damage characteristics(size and degree of difficulty) of coal.

Wearable Healthcare and Continuous Vital Sign Monitoring with IoT Integration

Technical and accessibility issues in hospitals often prevent patients from receiving optimal mental and physical health care,which is essential for independent living,especially as societies age and chronic diseases like diabetes and cardiovascular disease become more common.Recent advances in the Internet of Things(IoT)-enabled wearable devices offer potential solutions for remote health monitoring and everyday activity recognition,gaining significant attention in personalized healthcare.This paper comprehensively reviews wearable healthcare technology integrated with the IoT for continuous vital sign monitoring.Relevant papers were extracted and analyzed using a systematic numerical review method,covering various aspects such as sports monitoring,disease detection,patient monitoring,and medical diagnosis.The review highlights the transformative impact of IoTenabled wearable devices in healthcare,facilitating real-time monitoring of vital signs,including blood pressure,temperature,oxygen levels,and heart rate.Results from the reviewed papers demonstrate high accuracy and efficiency in predicting health conditions,improving sports performance,enhancing patient care,and diagnosing diseases.The integration of IoT in wearable healthcare devices enables remote patient monitoring,personalized care,and efficient data transmission,ultimately transcending traditional boundaries of healthcare and leading to better patient outcomes.

Enhancing production monitoring: A back allocation methodology to estimate well flow rates and assist well test scheduling

Production flow rates are crucial to make operational decisions,monitor,manage,and optimize oil and gas fields.Flow rates also have a financial importance to correctly allocate production to fiscal purposes required by regulatory agencies or to allocate production in fields owned by multiple operators.Despite its significance,usually only the total field production is measured in real time,which requires an alternative way to estimate wells'production.To address these challenges,this work presents a back allocation methodology that leverages real-time instrumentation,simulations,algorithms,and mathe-matical programming modeling to enhance well monitoring and assist in well test scheduling.The methodology comprises four modules:simulation,classification,error calculation,and optimization.These modules work together to characterize the flowline,wellbore,and reservoir,verify simulation outputs,minimize errors,and calculate flow rates while honoring the total platform flow rate.The well status generated through the classification module provides valuable information about the current condition of each well(i.e.if the well is deviating from the latest well test parameters),aiding in decision-making for well testing scheduling and prioritizing.The effectiveness of the methodology is demonstrated through its application to a representative offshore oil field with 14 producing wells and two years of daily production data.The results highlight the robustness of the methodology in properly classifying the wells and obtaining flow rates that honor the total platform flow rate.Furthermore,the methodology supports well test scheduling and provides reliable indicators for well conditions.By uti-lizing real-time data and advanced modeling techniques,this methodology enhances production monitoring and facilitates informed operational decision-making in the oil and gas industry.

Semi-implantable device based on multiplexed microfilament electrode cluster for continuous monitoring of physiological ions

Modern medicine is increasingly interested in advanced sensors to detect and analyze biochemical indicators.Ion sensors based on potentiometric methods are a promising platform for monitoring physiological ions in biological subjects.Current semi-implantable devices are mainly based on single-parameter detection.Miniaturized semi-implantable electrodes for multiparameter sensing have more restrictions on the electrode size due to biocompatibility considerations,but reducing the electrode surface area could potentially limit electrode sensitivity.This study developed a semi-implantable device system comprising a multiplexed microfilament electrode cluster(MMEC)and a printed circuit board for real-time monitoring of intra-tissue K^(+),Ca^(2+),and Na^(+)concentrations.The electrode surface area was less important for the potentiometric sensing mechanism,suggesting the feasibility of using a tiny fiber-like electrode for potentiometric sensing.The MMEC device exhibited a broad linear response(K^(+):2–32 mmol/L;Ca^(2+):0.5–4 mmol/L;Na^(+):10–160 mmol/L),high sensitivity(about 20–45 mV/decade),temporal stability(>2weeks),and good selectivity(>80%)for the above ions.In vitro detection and in vivo subcutaneous and brain experiment results showed that the MMEC system exhibits good multi-ion monitoring performance in several complex environments.This work provides a platform for the continuous real-time monitoring of ion fluctuations in different situations and has implications for developing smart sensors to monitor human health.