Appllcation of formaldehyde red cell in HA and HI for pigeon Paramyxovirus type l was descrital and a comporison was made with fresh chicken red cell. ZH strain of pigeon A/PMV- lwas used to infect 9 - 11 day old chic...Appllcation of formaldehyde red cell in HA and HI for pigeon Paramyxovirus type l was descrital and a comporison was made with fresh chicken red cell. ZH strain of pigeon A/PMV- lwas used to infect 9 - 11 day old chicken embryoes for the preparation of allantoic fluid antigen and to immunize Pigeons to PrePare PoSitive serum. 50 allantoic fluids and 55 sera were examined by HA and HI both with formaldehyde red cell and fresh chicken red cell. It was shown that results obtuined with the two red cells were basically the same and formaldehyde red cell is more convenient to use and hence can replace fresh chicken red cell in conducting HA and HI for the detection of pigeon poramyxovirus type l and other展开更多
Virion RNA was abstracted from purified type I Avian paramyxovirus strain YN-PA01(isolated from parrot)and used as a template. The fragment containing the fusion gene(F) and hemagglutinin-neuraminidase gene(HN) of the...Virion RNA was abstracted from purified type I Avian paramyxovirus strain YN-PA01(isolated from parrot)and used as a template. The fragment containing the fusion gene(F) and hemagglutinin-neuraminidase gene(HN) of the isolate was amplified by RT-PCR and cloned to the pMD18-T Vector. Using primer walking method the complete sequence of F-HN genes was obtained finally.And the respective amino acid sequence was deduced. Through relative software the phylogenetic trees on F gene and HN gene were constructed between strain YN-PA01 and reference strains. The results showed that strain YN-PA01 comparing with reference strains displays 98.7%-83.2% nucleotide homology and 98.1%-86.2% amino acid homology on F gene; 97.4%-79.1% nucleotide homology and 97.2%-83.2% amino acid homology on HN gene. Additional 6 amino acids are encoded by the HN gene ORF of strain YN-PA01 comparing with national reference strains.The studied strain YN-PA01 exhibits highest identities with strain JS/5[O1/Go either analyzed on F gene or HN gene.展开更多
文摘Appllcation of formaldehyde red cell in HA and HI for pigeon Paramyxovirus type l was descrital and a comporison was made with fresh chicken red cell. ZH strain of pigeon A/PMV- lwas used to infect 9 - 11 day old chicken embryoes for the preparation of allantoic fluid antigen and to immunize Pigeons to PrePare PoSitive serum. 50 allantoic fluids and 55 sera were examined by HA and HI both with formaldehyde red cell and fresh chicken red cell. It was shown that results obtuined with the two red cells were basically the same and formaldehyde red cell is more convenient to use and hence can replace fresh chicken red cell in conducting HA and HI for the detection of pigeon poramyxovirus type l and other
文摘Virion RNA was abstracted from purified type I Avian paramyxovirus strain YN-PA01(isolated from parrot)and used as a template. The fragment containing the fusion gene(F) and hemagglutinin-neuraminidase gene(HN) of the isolate was amplified by RT-PCR and cloned to the pMD18-T Vector. Using primer walking method the complete sequence of F-HN genes was obtained finally.And the respective amino acid sequence was deduced. Through relative software the phylogenetic trees on F gene and HN gene were constructed between strain YN-PA01 and reference strains. The results showed that strain YN-PA01 comparing with reference strains displays 98.7%-83.2% nucleotide homology and 98.1%-86.2% amino acid homology on F gene; 97.4%-79.1% nucleotide homology and 97.2%-83.2% amino acid homology on HN gene. Additional 6 amino acids are encoded by the HN gene ORF of strain YN-PA01 comparing with national reference strains.The studied strain YN-PA01 exhibits highest identities with strain JS/5[O1/Go either analyzed on F gene or HN gene.
