IBL即Industry Based Learning,意为基于工业需要的学习,核心思想是从工业企业实际生产需求出发,培养企业工程师的工程实践能力。本文提出的工程师设计制造能力的提升立足于普通高校校内资源,通过自身软硬件建设满足教学要求,按照教学...IBL即Industry Based Learning,意为基于工业需要的学习,核心思想是从工业企业实际生产需求出发,培养企业工程师的工程实践能力。本文提出的工程师设计制造能力的提升立足于普通高校校内资源,通过自身软硬件建设满足教学要求,按照教学计划要求,根据教学大纲规定的内容和质量要求进行教学和考核,质量可控,满足现有招生学生全部的实践要求。展开更多
Solid state bio-processing of wheat straw was carried out through an indigenous fungal strain Pleurotus ostreatus IBL-02 under pre-optimized fermentation conditions. The maximum activity, 692±12 U/mL, of the indu...Solid state bio-processing of wheat straw was carried out through an indigenous fungal strain Pleurotus ostreatus IBL-02 under pre-optimized fermentation conditions. The maximum activity, 692±12 U/mL, of the industrially important manganese peroxidase (MnP) enzyme was recorded after five days of still culture incubation. The crude MnP was 2.1-fold purified with a specific activity of 860 U/mg after purification on a Sephadex-G-100 gel column. On native and SDS-PAGE electrophoresis gels, the purified MnP fraction was a single homogenous band of 45 kDa. An active fraction of MnP was immobilized using hydrophobic sol-gel entrapment comprising tetramethoxysilane (T) and propyltrimethoxysilane (P) at different T:P molar ratios. Characterization revealed that after 24 h incubation at varying pH and temperatures, the MnP fraction immobilized at a T:P ratio of 1:2 in the sol-gel retained 82% and 75% of its original activity at pH4 and 70 ℃, respectively. The optimally active fraction at a 1:2 T:P ratio was tested against MnSO4 as a substrate to determine the kinetic catalytic constants KM and Vmax . To explore the industrial applicability of P. ostreatus IBL-02 MnP, both the free and immobilized MnP were used for the decolorization of four different textile industrial effluents. A maximum of 100% decolorization was achieved for the different textile effluents within the shortest time period. A lower KM , higher Vmax , hyper-activation, and enhanced acidic and thermal resistance up to 70 ℃ were the novel catalytic features of the sol-gel immobilized MnP, suggesting that it may be a potential candidate for biotechnological applications particularly for textile bioremediation purposes.展开更多
We study the large-time behavior toward viscous shock waves to the Cauchy problem of the one-dimensional compressible isentropic Navier-Stokes equations with density- dependent viscosity. The nonlinear stability of th...We study the large-time behavior toward viscous shock waves to the Cauchy problem of the one-dimensional compressible isentropic Navier-Stokes equations with density- dependent viscosity. The nonlinear stability of the viscous shock waves is shown for certain class of large initial perturbation with integral zero which can allow the initial density to have large oscillation. Our analysis relies upon the technique developed by Kanel~ and the continuation argument.展开更多
文摘IBL即Industry Based Learning,意为基于工业需要的学习,核心思想是从工业企业实际生产需求出发,培养企业工程师的工程实践能力。本文提出的工程师设计制造能力的提升立足于普通高校校内资源,通过自身软硬件建设满足教学要求,按照教学计划要求,根据教学大纲规定的内容和质量要求进行教学和考核,质量可控,满足现有招生学生全部的实践要求。
基金a part of a research project entitled "The development of immobilized ligninolytic enzymes for industrial applications" supported by Higher Education Commission (HEC), Islamabad, Pakistan
基金a part of a research project entitled “the development of immobilized ligninolytic enzymes for industrial applications” supported by Higher Education Commission (HEC), Islamabad, Pakistan
文摘Solid state bio-processing of wheat straw was carried out through an indigenous fungal strain Pleurotus ostreatus IBL-02 under pre-optimized fermentation conditions. The maximum activity, 692±12 U/mL, of the industrially important manganese peroxidase (MnP) enzyme was recorded after five days of still culture incubation. The crude MnP was 2.1-fold purified with a specific activity of 860 U/mg after purification on a Sephadex-G-100 gel column. On native and SDS-PAGE electrophoresis gels, the purified MnP fraction was a single homogenous band of 45 kDa. An active fraction of MnP was immobilized using hydrophobic sol-gel entrapment comprising tetramethoxysilane (T) and propyltrimethoxysilane (P) at different T:P molar ratios. Characterization revealed that after 24 h incubation at varying pH and temperatures, the MnP fraction immobilized at a T:P ratio of 1:2 in the sol-gel retained 82% and 75% of its original activity at pH4 and 70 ℃, respectively. The optimally active fraction at a 1:2 T:P ratio was tested against MnSO4 as a substrate to determine the kinetic catalytic constants KM and Vmax . To explore the industrial applicability of P. ostreatus IBL-02 MnP, both the free and immobilized MnP were used for the decolorization of four different textile industrial effluents. A maximum of 100% decolorization was achieved for the different textile effluents within the shortest time period. A lower KM , higher Vmax , hyper-activation, and enhanced acidic and thermal resistance up to 70 ℃ were the novel catalytic features of the sol-gel immobilized MnP, suggesting that it may be a potential candidate for biotechnological applications particularly for textile bioremediation purposes.
基金supported by"the Fundamental Research Funds for the Central Universities"
文摘We study the large-time behavior toward viscous shock waves to the Cauchy problem of the one-dimensional compressible isentropic Navier-Stokes equations with density- dependent viscosity. The nonlinear stability of the viscous shock waves is shown for certain class of large initial perturbation with integral zero which can allow the initial density to have large oscillation. Our analysis relies upon the technique developed by Kanel~ and the continuation argument.
