In this study, we, for the first time, tried to apply IC50 values (inhibitory concentration estimated to affect the endpoint in question by 50%) in the MTT colorimetric assay to investigate the cytotoxic effects of hi...In this study, we, for the first time, tried to apply IC50 values (inhibitory concentration estimated to affect the endpoint in question by 50%) in the MTT colorimetric assay to investigate the cytotoxic effects of highly absorbent foam dressings based on silver zirconium phosphate, a newly nano-based matrix. Our results showed that silver released from dressings based on silver zirconium phosphate attributed mainly to highly cytotoxic to L929 cells cultured with MEM containing 10% fetal bovine serum. In addition, we have also compared the IC<sub>50</sub> values among different dilutions of AgNO<sub>3</sub> solution, silver based dressing extracts and material reference control (ZDEC) extracts using the optimized MTT assay, along with characterizing the silver content in the dressing extracts using atomic absorption spectroscopy. Results have shown that the IC<sub>50</sub> values of AgNO<sub>50</sub> solution, silver based dressing extracts and ZDEC extracts are 3.5 μg/mL, 3.8 μg/mL and 8.4%, respectively. And there exist some good agreements between qualitative and quantitative evaluation method as well. In conclusion, our study has led to the view that the IC<sub>50</sub> value is a promising quantitative index for screening cytotoxicity with regard to silver based dressings.展开更多
为了探讨5-甲基胞嘧啶(5-methylcytosine,m5C)相关基因在三阴性乳腺癌(triple negative breast cancer,TNBC)患者治疗及预后中的潜在价值,构建了基于m5C相关基因的预后预测模型,用于评估TNBC患者的预后和生存状况。从基因表达总库(gene ...为了探讨5-甲基胞嘧啶(5-methylcytosine,m5C)相关基因在三阴性乳腺癌(triple negative breast cancer,TNBC)患者治疗及预后中的潜在价值,构建了基于m5C相关基因的预后预测模型,用于评估TNBC患者的预后和生存状况。从基因表达总库(gene expression omnibus,GEO)数据库和癌症基因组图谱(the cancer genome atlas,TCGA)数据库中下载TNBC基因表达谱和相应的临床数据。通过Pearson分析确定了99个m5C相关基因,进一步采用单因素Cox分析鉴定出5个与预后有关的m5C相关基因(SLC6A14、BCL11A、UGT8、LMO4、PSAT1)并构建了风险评分(risk score)预测模型,根据风险评分中位值将患者划分为高风险组和低风险组。使用Kaplan-Meier(K-M)生存分析、受试者工作特征(receiver operating characteristic,ROC)曲线、多变量Cox回归分析、构建列线图和校准曲线评估了模型的预测效能。训练集和验证集的K-M生存曲线、受试者工作特征曲线下面积(area under the curve,AUC)分析均验证了模型具有良好的预测能力。多变量Cox回归分析显示,风险评分可作为独立的预后生物标志物。使用ssGSEA、免疫评分分析和化疗药物对高低风险组患者的半最大抑制浓度(half maximal inhibitory concentration,IC50)值差异分析显示,免疫细胞和免疫检查点基因以及大多数化疗药物的IC50值在不同风险组之间的表达存在显著差异。研究结果构建了基于5个m5C相关基因的风险评分预后预测模型,这将有助于阐明TNBC中m5C相关基因的作用机制,进而提供更有价值的预后及诊断的生物标志物和潜在的治疗靶点,为TNBC患者临床个性化治疗提供理论指导。展开更多
[ Objective ] The paper was to systemically study the characteristics of acetyl cholinesterase （ACHE） in Pardosa astrigera, and confirm the occurrence and development of its pesticide resistance and the resistance l...[ Objective ] The paper was to systemically study the characteristics of acetyl cholinesterase （ACHE） in Pardosa astrigera, and confirm the occurrence and development of its pesticide resistance and the resistance level, thereby establishing a quick and accurate detection method for enzyme activity. [ Method] The optimal conditions for assaying the activity of AChE in different parts of P. astrigera were determined by orthogonal experiment. The distribution conditions of acetyl cholinesterase （AChE） in different tissues of P. astrigera were further studied, and the sensitivities of the enzyme tO four common pesticides were also determined. [ Result] The optimal condition for assaying the activity of AChE in cephalothorax, abdomen and appendage of P. astrigera was as follows, enzyme concentrations： 12, 18 and 29 g/L; substrate concentrations： 0.6, 1.0 and 1.0 mmol/L; pH value, 7.0; reaction temperatures： 30, 35, 35℃ ; reaction time, 5 rain. AChE was mainly distributed in the cephalothorax of P. astrigera. The specific activity of AChE in the extract solution with Triton X-100 was higher than that in the solution without Triton X-100. The median inhibitory concentrations （IC50） of methomyl, phoxim, betacypermethrin, chlorpyrifos against AChE in the cephalothorax of P. astrigera were 7.76 × 10^-5, 1.76×10^-4, 4.12 ×10^-4 and 4.94 ×10^-4 mol/L, respectively. [ Conclusion] AChEs in P. astrigera were membrane-bounded. The inhibition of four pesticides against AChE in the cephalothorax of P. astrigera had good dese-effect, this indicated that AChE in the cephalothorax of P. astrig- era could be used as the biochemical marker to monitor the contamination of organic phosphorus, carbamate and pyrethroid pesticides in the environment.展开更多
The extracts from different tissues of Allium fistulosum L. and Allium sativum L. were investigated to evaluate their antioxidant and antimicrobial capacity. The highest yields of the Allium extracts were prepared fro...The extracts from different tissues of Allium fistulosum L. and Allium sativum L. were investigated to evaluate their antioxidant and antimicrobial capacity. The highest yields of the Allium extracts were prepared from the extraction of 30% ethanol solution. The DPPH scavenging activity was the highest in A. fistulosum L. leaves, which IC50 is 14.61 μg·mL-1. The highest antioxidant activity using TEAC assay and total phenolic content were observed in A. sativum L. stems and A. fistulosum L. stems, where they are determined to be 15.51 mMand 191.04 mg GAE·g-14.59 mMand 182.60 mg GAE·g-1,respectively. Statistic analysis revealed that the DPPH IC50 value was significantly correlated with total phenolic contents and antioxidant activity using TEAC assay. The extracts of A. sativum L. bulbs were found to exhibit antimicrobial activity against Staphylococcus aureus with the MIC and MBC of 0.2 mg·mL-1and 0.4 mg·mL-1, respectively. In addition, the extract of A. fistulosum L. stems was more active against Bacillus subtilis, with an MIC and MBC of 0.2 and 0.4 mg·mL-1, respectively. The inhibitory activity of various Allium extracts against the test bacteria was greater than that of 10 μg·mL-1 allicin. The results indicated that Allium spp. extracts could be used as a potential source of natural antioxidants and antimicrobial agents.展开更多
文摘In this study, we, for the first time, tried to apply IC50 values (inhibitory concentration estimated to affect the endpoint in question by 50%) in the MTT colorimetric assay to investigate the cytotoxic effects of highly absorbent foam dressings based on silver zirconium phosphate, a newly nano-based matrix. Our results showed that silver released from dressings based on silver zirconium phosphate attributed mainly to highly cytotoxic to L929 cells cultured with MEM containing 10% fetal bovine serum. In addition, we have also compared the IC<sub>50</sub> values among different dilutions of AgNO<sub>3</sub> solution, silver based dressing extracts and material reference control (ZDEC) extracts using the optimized MTT assay, along with characterizing the silver content in the dressing extracts using atomic absorption spectroscopy. Results have shown that the IC<sub>50</sub> values of AgNO<sub>50</sub> solution, silver based dressing extracts and ZDEC extracts are 3.5 μg/mL, 3.8 μg/mL and 8.4%, respectively. And there exist some good agreements between qualitative and quantitative evaluation method as well. In conclusion, our study has led to the view that the IC<sub>50</sub> value is a promising quantitative index for screening cytotoxicity with regard to silver based dressings.
文摘为了探讨5-甲基胞嘧啶(5-methylcytosine,m5C)相关基因在三阴性乳腺癌(triple negative breast cancer,TNBC)患者治疗及预后中的潜在价值,构建了基于m5C相关基因的预后预测模型,用于评估TNBC患者的预后和生存状况。从基因表达总库(gene expression omnibus,GEO)数据库和癌症基因组图谱(the cancer genome atlas,TCGA)数据库中下载TNBC基因表达谱和相应的临床数据。通过Pearson分析确定了99个m5C相关基因,进一步采用单因素Cox分析鉴定出5个与预后有关的m5C相关基因(SLC6A14、BCL11A、UGT8、LMO4、PSAT1)并构建了风险评分(risk score)预测模型,根据风险评分中位值将患者划分为高风险组和低风险组。使用Kaplan-Meier(K-M)生存分析、受试者工作特征(receiver operating characteristic,ROC)曲线、多变量Cox回归分析、构建列线图和校准曲线评估了模型的预测效能。训练集和验证集的K-M生存曲线、受试者工作特征曲线下面积(area under the curve,AUC)分析均验证了模型具有良好的预测能力。多变量Cox回归分析显示,风险评分可作为独立的预后生物标志物。使用ssGSEA、免疫评分分析和化疗药物对高低风险组患者的半最大抑制浓度(half maximal inhibitory concentration,IC50)值差异分析显示,免疫细胞和免疫检查点基因以及大多数化疗药物的IC50值在不同风险组之间的表达存在显著差异。研究结果构建了基于5个m5C相关基因的风险评分预后预测模型,这将有助于阐明TNBC中m5C相关基因的作用机制,进而提供更有价值的预后及诊断的生物标志物和潜在的治疗靶点,为TNBC患者临床个性化治疗提供理论指导。
基金Supported by Shanxi Scholarship Fund(2009042)Youth Science andTechnology Research Fund in Shanxi Province(2008021041)~~
文摘[ Objective ] The paper was to systemically study the characteristics of acetyl cholinesterase （ACHE） in Pardosa astrigera, and confirm the occurrence and development of its pesticide resistance and the resistance level, thereby establishing a quick and accurate detection method for enzyme activity. [ Method] The optimal conditions for assaying the activity of AChE in different parts of P. astrigera were determined by orthogonal experiment. The distribution conditions of acetyl cholinesterase （AChE） in different tissues of P. astrigera were further studied, and the sensitivities of the enzyme tO four common pesticides were also determined. [ Result] The optimal condition for assaying the activity of AChE in cephalothorax, abdomen and appendage of P. astrigera was as follows, enzyme concentrations： 12, 18 and 29 g/L; substrate concentrations： 0.6, 1.0 and 1.0 mmol/L; pH value, 7.0; reaction temperatures： 30, 35, 35℃ ; reaction time, 5 rain. AChE was mainly distributed in the cephalothorax of P. astrigera. The specific activity of AChE in the extract solution with Triton X-100 was higher than that in the solution without Triton X-100. The median inhibitory concentrations （IC50） of methomyl, phoxim, betacypermethrin, chlorpyrifos against AChE in the cephalothorax of P. astrigera were 7.76 × 10^-5, 1.76×10^-4, 4.12 ×10^-4 and 4.94 ×10^-4 mol/L, respectively. [ Conclusion] AChEs in P. astrigera were membrane-bounded. The inhibition of four pesticides against AChE in the cephalothorax of P. astrigera had good dese-effect, this indicated that AChE in the cephalothorax of P. astrig- era could be used as the biochemical marker to monitor the contamination of organic phosphorus, carbamate and pyrethroid pesticides in the environment.
文摘The extracts from different tissues of Allium fistulosum L. and Allium sativum L. were investigated to evaluate their antioxidant and antimicrobial capacity. The highest yields of the Allium extracts were prepared from the extraction of 30% ethanol solution. The DPPH scavenging activity was the highest in A. fistulosum L. leaves, which IC50 is 14.61 μg·mL-1. The highest antioxidant activity using TEAC assay and total phenolic content were observed in A. sativum L. stems and A. fistulosum L. stems, where they are determined to be 15.51 mMand 191.04 mg GAE·g-14.59 mMand 182.60 mg GAE·g-1,respectively. Statistic analysis revealed that the DPPH IC50 value was significantly correlated with total phenolic contents and antioxidant activity using TEAC assay. The extracts of A. sativum L. bulbs were found to exhibit antimicrobial activity against Staphylococcus aureus with the MIC and MBC of 0.2 mg·mL-1and 0.4 mg·mL-1, respectively. In addition, the extract of A. fistulosum L. stems was more active against Bacillus subtilis, with an MIC and MBC of 0.2 and 0.4 mg·mL-1, respectively. The inhibitory activity of various Allium extracts against the test bacteria was greater than that of 10 μg·mL-1 allicin. The results indicated that Allium spp. extracts could be used as a potential source of natural antioxidants and antimicrobial agents.
