目的:对HL60细胞在NSC67657作用下向单核系分化前后,双向电泳分离的表达差异蛋白β-catenin相关蛋白1(Beta-catenin-interacting protein 1,ICAT)进行验证,并对ICAT在细胞分化中的功能进行研究.方法:通过RT-PCR和Western blot方法验证...目的:对HL60细胞在NSC67657作用下向单核系分化前后,双向电泳分离的表达差异蛋白β-catenin相关蛋白1(Beta-catenin-interacting protein 1,ICAT)进行验证,并对ICAT在细胞分化中的功能进行研究.方法:通过RT-PCR和Western blot方法验证药物作用细胞前后ICAT基因和蛋白的表达差异;通过免疫荧光协同分析目的蛋白的表达水平,并对其进行初步定位.构建pDsRed-ICAT真核表达载体,转染HL60细胞,筛选阳性克隆.对ICAT基因重组质粒转染细胞作细胞形态学、细胞增殖改变的观察和细胞周期检测以及超微结构观察.结果:NSC67657诱导HL60细胞向单核系分化,ICAT蛋白表达上调,其主要定位于细胞核和胞质.真核表达载体构建成功,电转后G418筛选可得90%以上阳性克隆.转染重组质粒的HL60细胞增殖受抑,电镜下胞核异染色质密集,核质比减小,表面抗原CD14表达和对照组无差异,但在药物处理后24h即可表达71.3%,明显高于对照组,瑞氏染色可见明显分化细胞.结论:ICAT蛋白在NSC67657诱导HL60细胞分化中表达上调,但仅是过表达的ICAT基因并不能诱导HL60细胞向单核系分化,却能提高HL60细胞对NSC67657诱导作用的敏感性.展开更多
AIM: To classify the histological severity of Helicobacter pylori (H. pylori) infection-associated gastritis by confocal laser endomicroscopy (CLE). METHODS: Patients with upper gastrointestinal symptoms or individual...AIM: To classify the histological severity of Helicobacter pylori (H. pylori) infection-associated gastritis by confocal laser endomicroscopy (CLE). METHODS: Patients with upper gastrointestinal symptoms or individuals who were screened for gastric cancer were enrolled in this study. Histological severity of H. pylori infection-associated gastritis was graded according to the established CLE criteria. Diagnostic value of CLE for histo-logical gastritis was investigated and compared with that of white light endoscopy (WLE). Targeted biopsies from the sites observed by CLE were performed. RESULTS: A total of 118 consecutive patients with H. pylori infection-associated gastritis were enrolled in this study. Receiver operating characteristic curve analysis showedthat the sensitivity and specifi city of CLE were 82.9% and 90.9% for the diagnosis of H. pylori infection, 94.6% and 97.4% for predicting gastric normal mucosa, 98.5% and 94.6% for predicting histological active inflammation, 92.9% and 95.2% for predicting glan-dular atrophy, 98.6% and 100% for diagnosing intes-tinal metaplasia, respectively. Post-CLE image analysis showed that goblet cells and absorptive cells were the two most common parameters on the CLE-diagnosed intestinal metaplasia (IM) images (P < 0.001). More his-tological lesions of the stomach could be found by CLE than by WLE (P < 0.001). CONCLUSION: CLE can accurately show the histological severity of H. pylori infection-associated gastritis. Mapping IM by CLE has a rather good diagnostic accuracy.展开更多
The nitrogen loss pathway in winter wheat and summer maize rotation system was studied based on field experimental data. The results showed that nitrogen recovery rate was significantly decreased with nitrogen fertili...The nitrogen loss pathway in winter wheat and summer maize rotation system was studied based on field experimental data. The results showed that nitrogen recovery rate was significantly decreased with nitrogen fertilization rate increased, while residual rate and losses rate had an increasing trend. Accumulated ammonia volatilization loss in winter wheat and summer maize rotation was 12. 8(N0), 22.0(N120), 33. 0(N240) and 64. 5 kg N ha-1 (N360) respectively and rate of ammonia volatilization loss was 3.8, 4.2 and 7.2% respectively while urea was mixed with 0 -10 cm soil or spread before irrigation. Denitrification loss with acetylene-soil core incubation method in winter wheat was lower than 1 kg N ha-1 and rate of denitrification loss was 0. 21 - 0. 26% or trace. Denitrification loss in summer maize was 1 - 14 kg N ha-1 and rate of denitrification loss was 1-5%. The total gaseous loss in winter wheat and summer maize rotation system was less than 10%, and the main nitrogen fertilizer loss way was leaching below 0 - 100 cm soil profile and accumulated in deeper soil.展开更多
The ways in which epigenetic modifications fix the effects of early environmental events,ensuring sustained responses to transient stimuli,which result in modified gene expression patterns and phenotypes later in life...The ways in which epigenetic modifications fix the effects of early environmental events,ensuring sustained responses to transient stimuli,which result in modified gene expression patterns and phenotypes later in life,is a topic of considerable interest.This review focuses on recently discovered mechanisms and calls into question prevailing views about the dynamics,position and functions of epigenetic marks.Most epigenetic studies have addressed the long-term effects on a small number of epigenetic marks,at the global or individual gene level,of environmental stressors in humans and animal models.In parallel,increasing numbers of studies based on high-throughput technologies and focusing on humans and mice have revealed additional complexity in epigenetic processes,by highlighting the importance of crosstalk between the different epigenetic marks.A number of studies focusing on the developmental origin of health and disease and metabolic programming have identified links between early nutrition,epigenetic processes and long-term illness.The existence of a self-propagating epigenetic cycle has been demonstrated.Moreover,recent studies demonstrate an obvious sexual dimorphism both for programming trajectories and in response to the same environmental insult.Despite recent progress,we are still far from understanding how,when and where environmental stressors disturb key epigenetic mechanisms.Thus,identifying the original key marks and their changes throughout development during an individual's lifetime or over several generations remains a challenging issue.展开更多
Severe mitral annular calcification(MAC)is frequently seen in patients with advanced age and chronic kidney disease,but it is rare in rheumatic heart disease(RHD).We hereby report a case of 45-year-old female with chr...Severe mitral annular calcification(MAC)is frequently seen in patients with advanced age and chronic kidney disease,but it is rare in rheumatic heart disease(RHD).We hereby report a case of 45-year-old female with chronic RHD,who had severe MAC and mitral regurgitation.Fluoroscopy revealed a "crown"-like severe calcification of the mitral annulus.Autopsy of the heart revealed a calcified posterior mitral annulus,fused commissures,and calcified nodules at the atrial aspect of the mitral valve.展开更多
文摘目的:对HL60细胞在NSC67657作用下向单核系分化前后,双向电泳分离的表达差异蛋白β-catenin相关蛋白1(Beta-catenin-interacting protein 1,ICAT)进行验证,并对ICAT在细胞分化中的功能进行研究.方法:通过RT-PCR和Western blot方法验证药物作用细胞前后ICAT基因和蛋白的表达差异;通过免疫荧光协同分析目的蛋白的表达水平,并对其进行初步定位.构建pDsRed-ICAT真核表达载体,转染HL60细胞,筛选阳性克隆.对ICAT基因重组质粒转染细胞作细胞形态学、细胞增殖改变的观察和细胞周期检测以及超微结构观察.结果:NSC67657诱导HL60细胞向单核系分化,ICAT蛋白表达上调,其主要定位于细胞核和胞质.真核表达载体构建成功,电转后G418筛选可得90%以上阳性克隆.转染重组质粒的HL60细胞增殖受抑,电镜下胞核异染色质密集,核质比减小,表面抗原CD14表达和对照组无差异,但在药物处理后24h即可表达71.3%,明显高于对照组,瑞氏染色可见明显分化细胞.结论:ICAT蛋白在NSC67657诱导HL60细胞分化中表达上调,但仅是过表达的ICAT基因并不能诱导HL60细胞向单核系分化,却能提高HL60细胞对NSC67657诱导作用的敏感性.
基金Supported by A Program from Clinical Projects of Ministry of Health of China (2007) and Taishan Scholar Program of Shandong Province
文摘AIM: To classify the histological severity of Helicobacter pylori (H. pylori) infection-associated gastritis by confocal laser endomicroscopy (CLE). METHODS: Patients with upper gastrointestinal symptoms or individuals who were screened for gastric cancer were enrolled in this study. Histological severity of H. pylori infection-associated gastritis was graded according to the established CLE criteria. Diagnostic value of CLE for histo-logical gastritis was investigated and compared with that of white light endoscopy (WLE). Targeted biopsies from the sites observed by CLE were performed. RESULTS: A total of 118 consecutive patients with H. pylori infection-associated gastritis were enrolled in this study. Receiver operating characteristic curve analysis showedthat the sensitivity and specifi city of CLE were 82.9% and 90.9% for the diagnosis of H. pylori infection, 94.6% and 97.4% for predicting gastric normal mucosa, 98.5% and 94.6% for predicting histological active inflammation, 92.9% and 95.2% for predicting glan-dular atrophy, 98.6% and 100% for diagnosing intes-tinal metaplasia, respectively. Post-CLE image analysis showed that goblet cells and absorptive cells were the two most common parameters on the CLE-diagnosed intestinal metaplasia (IM) images (P < 0.001). More his-tological lesions of the stomach could be found by CLE than by WLE (P < 0.001). CONCLUSION: CLE can accurately show the histological severity of H. pylori infection-associated gastritis. Mapping IM by CLE has a rather good diagnostic accuracy.
基金the project of National Natural Science Foundation of China(30270787)the Key Technologies Research and Development Program(2002BA516A02) the Foundation of Ecological Process Laboratory in Institute of Applied Ecology.
文摘The nitrogen loss pathway in winter wheat and summer maize rotation system was studied based on field experimental data. The results showed that nitrogen recovery rate was significantly decreased with nitrogen fertilization rate increased, while residual rate and losses rate had an increasing trend. Accumulated ammonia volatilization loss in winter wheat and summer maize rotation was 12. 8(N0), 22.0(N120), 33. 0(N240) and 64. 5 kg N ha-1 (N360) respectively and rate of ammonia volatilization loss was 3.8, 4.2 and 7.2% respectively while urea was mixed with 0 -10 cm soil or spread before irrigation. Denitrification loss with acetylene-soil core incubation method in winter wheat was lower than 1 kg N ha-1 and rate of denitrification loss was 0. 21 - 0. 26% or trace. Denitrification loss in summer maize was 1 - 14 kg N ha-1 and rate of denitrification loss was 1-5%. The total gaseous loss in winter wheat and summer maize rotation system was less than 10%, and the main nitrogen fertilizer loss way was leaching below 0 - 100 cm soil profile and accumulated in deeper soil.
基金Supported by Grants from INRA,INSERM (ATC-Nutrition,PRNH)Association Franaise des Diabétiques+3 种基金the Institut Benjamin Delessertthe Fondation Coeur et Artères (FCA N° 05-T4)the Agence Nationale pour la Recherche (ANR 06-PNRA-022-01)Contrat Cadre d’Aide au Projet d’Innova-tion Stratégique Industrielle "IT-Diab"OSEO-ISI (ISI IT-DIAB-18/12/2008)
文摘The ways in which epigenetic modifications fix the effects of early environmental events,ensuring sustained responses to transient stimuli,which result in modified gene expression patterns and phenotypes later in life,is a topic of considerable interest.This review focuses on recently discovered mechanisms and calls into question prevailing views about the dynamics,position and functions of epigenetic marks.Most epigenetic studies have addressed the long-term effects on a small number of epigenetic marks,at the global or individual gene level,of environmental stressors in humans and animal models.In parallel,increasing numbers of studies based on high-throughput technologies and focusing on humans and mice have revealed additional complexity in epigenetic processes,by highlighting the importance of crosstalk between the different epigenetic marks.A number of studies focusing on the developmental origin of health and disease and metabolic programming have identified links between early nutrition,epigenetic processes and long-term illness.The existence of a self-propagating epigenetic cycle has been demonstrated.Moreover,recent studies demonstrate an obvious sexual dimorphism both for programming trajectories and in response to the same environmental insult.Despite recent progress,we are still far from understanding how,when and where environmental stressors disturb key epigenetic mechanisms.Thus,identifying the original key marks and their changes throughout development during an individual's lifetime or over several generations remains a challenging issue.
文摘Severe mitral annular calcification(MAC)is frequently seen in patients with advanced age and chronic kidney disease,but it is rare in rheumatic heart disease(RHD).We hereby report a case of 45-year-old female with chronic RHD,who had severe MAC and mitral regurgitation.Fluoroscopy revealed a "crown"-like severe calcification of the mitral annulus.Autopsy of the heart revealed a calcified posterior mitral annulus,fused commissures,and calcified nodules at the atrial aspect of the mitral valve.
