目的探讨柴胡皂苷D调控CaMKKβ/AMPK信号通路,在功能性消化不良中对胃肠道Cajal间质细胞(Interstitial cells of Cajal,ICCs)细胞自噬的作用及机制。方法分离大鼠原代ICCs细胞,谷氨酸刺激构建ICCs自噬模型,免疫荧光检测Ca2+水平。将原代...目的探讨柴胡皂苷D调控CaMKKβ/AMPK信号通路,在功能性消化不良中对胃肠道Cajal间质细胞(Interstitial cells of Cajal,ICCs)细胞自噬的作用及机制。方法分离大鼠原代ICCs细胞,谷氨酸刺激构建ICCs自噬模型,免疫荧光检测Ca2+水平。将原代ICCs细胞分为对照组、模型组、模型+柴胡皂苷D组、模型+CaMKKβ抑制剂组、模型+柴胡皂苷D+CaMKKβ抑制剂组。透射电镜观察自噬体超微结构,ELISA检测Ghrelin和SP的水平,免疫荧光检测Ca2+和LC-3Ⅱ的表达,Western blot检测LC-3Ⅱ/Ⅰ、CaMKKβ、p-AMPK、Drp1、MFN2、IP3R和RyR的蛋白表达水平。结果谷氨酸诱导的模型组ICCs中LC-3Ⅱ荧光表达增强。柴胡皂苷D干预可降低Ca2+浓度,降低CaMKKβ、AMPK和MFN2水平(P<0.01),增加LC-3Ⅱ/Ⅰ、IP3R、RyR、Drp1、Ghrelin和SP水平(P<0.01)。柴胡皂苷D联合CaMKKβ抑制剂STO-609干预后效果更显著。结论柴胡皂苷D可通过CaMKKβ/AMPK信号通路介导Ca2+外流,影响ICCs细胞过度自噬及胃肠动力相关因子的表达。展开更多
作业成本法（Activity Based Costing，简称ABC）以其独特的“作业消耗资源，产品消耗作业”的成本理念，使得传统成本会计系统失真的产品成本信息得以矫正，大大提高了成本信息的决策有用性。目前，ABC已经得到了迅猛的应用和发展。然...作业成本法（Activity Based Costing，简称ABC）以其独特的“作业消耗资源，产品消耗作业”的成本理念，使得传统成本会计系统失真的产品成本信息得以矫正，大大提高了成本信息的决策有用性。目前，ABC已经得到了迅猛的应用和发展。然而，ABC计算的成本信息只包含了经营成本，没有包含债务资本成本和权益资本成本，展开更多
This article focused on the application of ethnography as a qualitative study in developing non-English majors’intercultural communication competence(ICC).The PEER model guided student researchers through the interde...This article focused on the application of ethnography as a qualitative study in developing non-English majors’intercultural communication competence(ICC).The PEER model guided student researchers through the interdependent phrases of preparing,engaging,evaluating and reflecting in the research procedure.In addition,the four components of Byram’s ICC model,namely,knowledge,skills,attitudes,and critical awareness,were used to assess students’ICC development.展开更多
Language is learned to communicate, but how to use it appropriately is far more important. Since language and culture have an inextricable link in between, to improve the students' Intercultural communicative comp...Language is learned to communicate, but how to use it appropriately is far more important. Since language and culture have an inextricable link in between, to improve the students' Intercultural communicative competence(ICC), issues on integrating an intercultural approach into ELT classroom has been long debated and studied in foreign language teaching field. This paper will start with the definition of ICC, analyze the correlation between language and culture then offer valuable suggestions on how to integrate Ic LL approach into ELT in China.展开更多
文摘目的探讨柴胡皂苷D调控CaMKKβ/AMPK信号通路,在功能性消化不良中对胃肠道Cajal间质细胞(Interstitial cells of Cajal,ICCs)细胞自噬的作用及机制。方法分离大鼠原代ICCs细胞,谷氨酸刺激构建ICCs自噬模型,免疫荧光检测Ca2+水平。将原代ICCs细胞分为对照组、模型组、模型+柴胡皂苷D组、模型+CaMKKβ抑制剂组、模型+柴胡皂苷D+CaMKKβ抑制剂组。透射电镜观察自噬体超微结构,ELISA检测Ghrelin和SP的水平,免疫荧光检测Ca2+和LC-3Ⅱ的表达,Western blot检测LC-3Ⅱ/Ⅰ、CaMKKβ、p-AMPK、Drp1、MFN2、IP3R和RyR的蛋白表达水平。结果谷氨酸诱导的模型组ICCs中LC-3Ⅱ荧光表达增强。柴胡皂苷D干预可降低Ca2+浓度,降低CaMKKβ、AMPK和MFN2水平(P<0.01),增加LC-3Ⅱ/Ⅰ、IP3R、RyR、Drp1、Ghrelin和SP水平(P<0.01)。柴胡皂苷D联合CaMKKβ抑制剂STO-609干预后效果更显著。结论柴胡皂苷D可通过CaMKKβ/AMPK信号通路介导Ca2+外流,影响ICCs细胞过度自噬及胃肠动力相关因子的表达。
文摘作业成本法（Activity Based Costing，简称ABC）以其独特的“作业消耗资源，产品消耗作业”的成本理念，使得传统成本会计系统失真的产品成本信息得以矫正，大大提高了成本信息的决策有用性。目前，ABC已经得到了迅猛的应用和发展。然而，ABC计算的成本信息只包含了经营成本，没有包含债务资本成本和权益资本成本，
文摘This article focused on the application of ethnography as a qualitative study in developing non-English majors’intercultural communication competence(ICC).The PEER model guided student researchers through the interdependent phrases of preparing,engaging,evaluating and reflecting in the research procedure.In addition,the four components of Byram’s ICC model,namely,knowledge,skills,attitudes,and critical awareness,were used to assess students’ICC development.
文摘Language is learned to communicate, but how to use it appropriately is far more important. Since language and culture have an inextricable link in between, to improve the students' Intercultural communicative competence(ICC), issues on integrating an intercultural approach into ELT classroom has been long debated and studied in foreign language teaching field. This paper will start with the definition of ICC, analyze the correlation between language and culture then offer valuable suggestions on how to integrate Ic LL approach into ELT in China.
