Cloud computing(CC)is an advanced technology that provides access to predictive resources and data sharing.The cloud environment represents the right type regarding cloud usage model ownership,size,and rights to acces...Cloud computing(CC)is an advanced technology that provides access to predictive resources and data sharing.The cloud environment represents the right type regarding cloud usage model ownership,size,and rights to access.It introduces the scope and nature of cloud computing.In recent times,all processes are fed into the system for which consumer data and cache size are required.One of the most security issues in the cloud environment is Distributed Denial of Ser-vice(DDoS)attacks,responsible for cloud server overloading.This proposed sys-tem ID3(Iterative Dichotomiser 3)Maximum Multifactor Dimensionality Posteriori Method(ID3-MMDP)is used to overcome the drawback and a rela-tively simple way to execute and for the detection of(DDoS)attack.First,the pro-posed ID3-MMDP method calls for the resources of the cloud platform and then implements the attack detection technology based on information entropy to detect DDoS attacks.Since because the entropy value can show the discrete or aggregated characteristics of the current data set,it can be used for the detection of abnormal dataflow,User-uploaded data,ID3-MMDP system checks and read risk measurement and processing,bug ratingfile size changes,orfile name changes and changes in the format design of the data size entropy value.Unique properties can be used whenever the program approaches any data error to detect abnormal data services.Finally,the experiment also verifies the DDoS attack detection capability algorithm.展开更多
目的探讨DNA分化抑制因子(inhibitor of DNA differentiation,Id)Id1/Id3在子宫内膜癌中的表达及对子宫内膜癌细胞增殖、侵袭、迁移及粘附等生物学行为的作用。方法 Western blot方法检测4例子宫内膜癌组织及相应的癌旁组织中的Id1/Id3...目的探讨DNA分化抑制因子(inhibitor of DNA differentiation,Id)Id1/Id3在子宫内膜癌中的表达及对子宫内膜癌细胞增殖、侵袭、迁移及粘附等生物学行为的作用。方法 Western blot方法检测4例子宫内膜癌组织及相应的癌旁组织中的Id1/Id3的表达;将子宫内膜癌RL-952和HEC-1-B两株细胞各分为未处理细胞组、空白病毒组、启动子病毒组和Id1/Id3双敲低组。通过qRT-PCR检测不同腺病毒载体感染细胞后MMP2、CXCR4和P21 mRNA表达量,Western blot检测MMP2、CXCR4、P21蛋白表达情况;通过MTT增殖试验、Transwell侵袭实验、细胞划痕实验和细胞粘附实验检测Id1/Id3表达改变后,对子宫内膜癌细胞增殖、侵袭、移行和粘附能力的影响。结果 Id1/Id3在子宫内膜癌组织中较癌旁组织表达增高(P<0.05);双敲低Id1/Id3,子宫内膜癌细胞中MMP2、CXCR4的mRNA水平和蛋白水平明显降低(P<0.05),P21的mRNA水平和蛋白水平则明显升高(P<0.05),RNAi组与其他3组细胞相比均有显著性差异;Id1/Id3双敲低组子宫内膜癌RL-952和HEC-1-B株细胞增殖能力、侵袭能力、迁移能力和粘附能力均抑制(P<0.05)。结论 Id1/Id3在子宫内膜癌患者组织中高表达,并可通过升调MMP2、CXCR4和降调P21的表达促进子宫内膜癌细胞增殖、侵袭、迁移及粘附作用,靶向Id1/Id3治疗可能成为预防和治疗子宫内膜癌的新方案。展开更多
文摘Cloud computing(CC)is an advanced technology that provides access to predictive resources and data sharing.The cloud environment represents the right type regarding cloud usage model ownership,size,and rights to access.It introduces the scope and nature of cloud computing.In recent times,all processes are fed into the system for which consumer data and cache size are required.One of the most security issues in the cloud environment is Distributed Denial of Ser-vice(DDoS)attacks,responsible for cloud server overloading.This proposed sys-tem ID3(Iterative Dichotomiser 3)Maximum Multifactor Dimensionality Posteriori Method(ID3-MMDP)is used to overcome the drawback and a rela-tively simple way to execute and for the detection of(DDoS)attack.First,the pro-posed ID3-MMDP method calls for the resources of the cloud platform and then implements the attack detection technology based on information entropy to detect DDoS attacks.Since because the entropy value can show the discrete or aggregated characteristics of the current data set,it can be used for the detection of abnormal dataflow,User-uploaded data,ID3-MMDP system checks and read risk measurement and processing,bug ratingfile size changes,orfile name changes and changes in the format design of the data size entropy value.Unique properties can be used whenever the program approaches any data error to detect abnormal data services.Finally,the experiment also verifies the DDoS attack detection capability algorithm.
文摘目的探讨DNA分化抑制因子(inhibitor of DNA differentiation,Id)Id1/Id3在子宫内膜癌中的表达及对子宫内膜癌细胞增殖、侵袭、迁移及粘附等生物学行为的作用。方法 Western blot方法检测4例子宫内膜癌组织及相应的癌旁组织中的Id1/Id3的表达;将子宫内膜癌RL-952和HEC-1-B两株细胞各分为未处理细胞组、空白病毒组、启动子病毒组和Id1/Id3双敲低组。通过qRT-PCR检测不同腺病毒载体感染细胞后MMP2、CXCR4和P21 mRNA表达量,Western blot检测MMP2、CXCR4、P21蛋白表达情况;通过MTT增殖试验、Transwell侵袭实验、细胞划痕实验和细胞粘附实验检测Id1/Id3表达改变后,对子宫内膜癌细胞增殖、侵袭、移行和粘附能力的影响。结果 Id1/Id3在子宫内膜癌组织中较癌旁组织表达增高(P<0.05);双敲低Id1/Id3,子宫内膜癌细胞中MMP2、CXCR4的mRNA水平和蛋白水平明显降低(P<0.05),P21的mRNA水平和蛋白水平则明显升高(P<0.05),RNAi组与其他3组细胞相比均有显著性差异;Id1/Id3双敲低组子宫内膜癌RL-952和HEC-1-B株细胞增殖能力、侵袭能力、迁移能力和粘附能力均抑制(P<0.05)。结论 Id1/Id3在子宫内膜癌患者组织中高表达,并可通过升调MMP2、CXCR4和降调P21的表达促进子宫内膜癌细胞增殖、侵袭、迁移及粘附作用,靶向Id1/Id3治疗可能成为预防和治疗子宫内膜癌的新方案。
