Objective To improve the detection rate of early gastric cancer by following the patients with gastric intraepithelial neoplasia (IEN) with gastroscopy at regular intervals. Methods A total of 326 patients with gastri...Objective To improve the detection rate of early gastric cancer by following the patients with gastric intraepithelial neoplasia (IEN) with gastroscopy at regular intervals. Methods A total of 326 patients with gastric IEN, including 289 with low grade IEN ( LGIEN) and 37 with high grade IEN ( HGIEN) confirmed by endoscopic biopsy from January 2013 to December 2017 were enrolled in this study. The patients with LGIEN received regular follow-ups, gastroscopy, pathological biopsy and treated by endoscopy or surgery when necessary. Mean-while ,the patients with HGIEN received treatment by endoscopy or surgery. The patients with gastric cancers were grouped by TNM staging by pathological examina-tion.展开更多
Cigarette smoking is a main source of cyanide in human body,which can be taken as a risk factor of cataract formation.In this study,combined gas chromatography and mass spectrum(GC/MS)was used todetermine the amino ac...Cigarette smoking is a main source of cyanide in human body,which can be taken as a risk factor of cataract formation.In this study,combined gas chromatography and mass spectrum(GC/MS)was used todetermine the amino acid hydantoin after the incubation of soluble humanlensγ-crystallins with cyanate.The carbamylated amino acids obtained bythis procedure are alanine and hlycine,which are N-terminal amino acids ofγ-crystallin,and leucine.The aggregate,which can be observed incarbamylatedγ_1-crystallin on SDS-PAGE,may be related to the formation of disulfide and non-disulfide covalent bonds,and it seems that Y₂and Yy-crystallins can not be aggregated to any great extend.The results in this study indicate that the GC/MS is an effective method for analyzing the carbamylation of lens proteins;y-crystallin may play a very important role in the formation of cataract associated with accumulation of cyanate in human body,such as heavy smoking.展开更多
文摘Objective To improve the detection rate of early gastric cancer by following the patients with gastric intraepithelial neoplasia (IEN) with gastroscopy at regular intervals. Methods A total of 326 patients with gastric IEN, including 289 with low grade IEN ( LGIEN) and 37 with high grade IEN ( HGIEN) confirmed by endoscopic biopsy from January 2013 to December 2017 were enrolled in this study. The patients with LGIEN received regular follow-ups, gastroscopy, pathological biopsy and treated by endoscopy or surgery when necessary. Mean-while ,the patients with HGIEN received treatment by endoscopy or surgery. The patients with gastric cancers were grouped by TNM staging by pathological examina-tion.
基金This work was supported by Chinese Medicine Foundation
文摘Cigarette smoking is a main source of cyanide in human body,which can be taken as a risk factor of cataract formation.In this study,combined gas chromatography and mass spectrum(GC/MS)was used todetermine the amino acid hydantoin after the incubation of soluble humanlensγ-crystallins with cyanate.The carbamylated amino acids obtained bythis procedure are alanine and hlycine,which are N-terminal amino acids ofγ-crystallin,and leucine.The aggregate,which can be observed incarbamylatedγ_1-crystallin on SDS-PAGE,may be related to the formation of disulfide and non-disulfide covalent bonds,and it seems that Y₂and Yy-crystallins can not be aggregated to any great extend.The results in this study indicate that the GC/MS is an effective method for analyzing the carbamylation of lens proteins;y-crystallin may play a very important role in the formation of cataract associated with accumulation of cyanate in human body,such as heavy smoking.
