目的:观察紫外线B(UVB)照射HeLa细胞后IEX-1 mRNA表达的变化。方法:常规培养人宫颈癌HeLa细胞,采用RT-PCR法检测剂量为0、2、4、8、16、32 m J/cm2的UVB照射30 s后HeLa细胞中IEX-1 mRNA的表达情况,同法检测剂量为32 m J/cm2的UVB照射后...目的:观察紫外线B(UVB)照射HeLa细胞后IEX-1 mRNA表达的变化。方法:常规培养人宫颈癌HeLa细胞,采用RT-PCR法检测剂量为0、2、4、8、16、32 m J/cm2的UVB照射30 s后HeLa细胞中IEX-1 mRNA的表达情况,同法检测剂量为32 m J/cm2的UVB照射后即刻及培养1、2、4、8、16 h时IEX-1 mRNA的表达情况。结果:随UVB剂量的升高,HeLa细胞IEX-1 mRNA的表达量呈升高的趋势(F=644.725,P<0.001)。32 m J/cm2的UVB照射HeLa细胞后即刻及培养1、2、4、8、16 h,IEX-1 mRNA的表达呈升高的趋势(F=56.249,P<0.001),培养4 h后IEX-1 mRNA表达水平达峰值。结论:HeLa细胞中IEX-1 mRNA的表达可被UVB迅速、短暂地诱导,且具有剂量依赖性。展开更多
即刻早期反应基因IEX-1(immediate early response gene X-1)作为即刻早期基因家族之一,参与细胞的多种生物学效应,其转录和表达受多种因素的调控如NF-κB、P53、SP1、AP2、1a-25(OH)2D3、TNF-α及电离辐射等。研究发现,IEX-1在多种肿...即刻早期反应基因IEX-1(immediate early response gene X-1)作为即刻早期基因家族之一,参与细胞的多种生物学效应,其转录和表达受多种因素的调控如NF-κB、P53、SP1、AP2、1a-25(OH)2D3、TNF-α及电离辐射等。研究发现,IEX-1在多种肿瘤细胞中转录及表达降低或缺失,而电离辐射等则可以激活诱导其在多种类型肿瘤细胞和正常细胞中快速的表达,其激活表达与辐射后细胞的生长、周期、凋亡等的改变密切相关。辐射可诱导特性使其具有潜在的临床和基因工程生产应用前景。展开更多
Objective To observe the apoptosis of osteosarcoma MG-63 cells induced by As2O3 and to explore its possible mechanisms. Methods The flowcytometric analysis and transmission electronmicroscope were performed to investi...Objective To observe the apoptosis of osteosarcoma MG-63 cells induced by As2O3 and to explore its possible mechanisms. Methods The flowcytometric analysis and transmission electronmicroscope were performed to investigate the inducing apoptosis and inhibitative of As2O3 on osteosarcoma MG-63 cells. In order to study mechanism of apoptosis in MG-63 cells treated with As2 O3, microarray was performed. The down-regulated gene was confirmed by RT-PCR, Northern-blotting. Results After treated with As2O3, hypodiploid peak before G0/G1 phase was observed in MG-63 cells through FCM analysis. Loss of microvilli, condensation and fragmentation of nuclear chromatin, condensation of cytoplasmic organelles, dilatation of the endoplasmic reticulum shrinkage of cells and alterations in cell membranes and apoptosis bodies which were observed in MG-63 cells treated with As2O3 by transmission electronmicroscope. The results of microarray show that As2 O3 induced MG-63 cell apoptosis involves down-regulation of IEX-1 and the down-regulated gene is confirmed by RT-PCR and Northern-blotting.Conclusion The results show that As2 O3 selectively inhibits growth of the solid tumor MG-63 cells by triggering apoptosis and indicates MG-63 induced by As2O3 cell apoptosis may through the IEX-1 pathway.展开更多
文摘即刻早期反应基因IEX-1(immediate early response gene X-1)作为即刻早期基因家族之一,参与细胞的多种生物学效应,其转录和表达受多种因素的调控如NF-κB、P53、SP1、AP2、1a-25(OH)2D3、TNF-α及电离辐射等。研究发现,IEX-1在多种肿瘤细胞中转录及表达降低或缺失,而电离辐射等则可以激活诱导其在多种类型肿瘤细胞和正常细胞中快速的表达,其激活表达与辐射后细胞的生长、周期、凋亡等的改变密切相关。辐射可诱导特性使其具有潜在的临床和基因工程生产应用前景。
文摘Objective To observe the apoptosis of osteosarcoma MG-63 cells induced by As2O3 and to explore its possible mechanisms. Methods The flowcytometric analysis and transmission electronmicroscope were performed to investigate the inducing apoptosis and inhibitative of As2O3 on osteosarcoma MG-63 cells. In order to study mechanism of apoptosis in MG-63 cells treated with As2 O3, microarray was performed. The down-regulated gene was confirmed by RT-PCR, Northern-blotting. Results After treated with As2O3, hypodiploid peak before G0/G1 phase was observed in MG-63 cells through FCM analysis. Loss of microvilli, condensation and fragmentation of nuclear chromatin, condensation of cytoplasmic organelles, dilatation of the endoplasmic reticulum shrinkage of cells and alterations in cell membranes and apoptosis bodies which were observed in MG-63 cells treated with As2O3 by transmission electronmicroscope. The results of microarray show that As2 O3 induced MG-63 cell apoptosis involves down-regulation of IEX-1 and the down-regulated gene is confirmed by RT-PCR and Northern-blotting.Conclusion The results show that As2 O3 selectively inhibits growth of the solid tumor MG-63 cells by triggering apoptosis and indicates MG-63 induced by As2O3 cell apoptosis may through the IEX-1 pathway.
