我院基于国际麻醉护理联盟(International Federation of Nurse Anesthetists,简称IFNA)教育培训基地,从麻醉专科护士培训基地的申请、学员资质审核、带教教师资质与审核、培训课程设置、考核内容与方式设定及培训后效果评价等方面,建...我院基于国际麻醉护理联盟(International Federation of Nurse Anesthetists,简称IFNA)教育培训基地,从麻醉专科护士培训基地的申请、学员资质审核、带教教师资质与审核、培训课程设置、考核内容与方式设定及培训后效果评价等方面,建设了南京地区麻醉护理专科护士培训基地。本文就这方面的基地建设和体会进行总结并为麻醉护理专科护士培训提供参考。展开更多
Dendritic cell (DC) maturation approved to be a pivotal process for initiating immunity. Many protocols were established in order the isolated peripheral blood mononuclear cells (PBMCs) from healthy donors to mature i...Dendritic cell (DC) maturation approved to be a pivotal process for initiating immunity. Many protocols were established in order the isolated peripheral blood mononuclear cells (PBMCs) from healthy donors to mature into dendritic cells (DCs). The purpose of this study was to present an effective and reliable DC maturation procedure by comparing three different protocols (Interleukin-4/Tumor Necrosis Factor-appha (IL-4/TNFa) DC protocol, Interferon alpha (IFNa) DC protocol and FAST DC protocol). Whole blood was collected from six healthy donors and PBMCs were isolated by Ficoll gradient centrifugation. The counted cells were incubated with the addition of three different cocktails of supplements for appropriate time period. The final mature DC population was examined either by its phenotypic characteristics under light microscope or by measuring the expression of antigen presenting molecules such as CD80 and CD86 by flow cytometry. It was found that the mature DCs, generated from the IL-4/TNFa DC protocol, expressed higher levels of CD80 and CD86. Furthermore, they sharply exhibited their phenotypic hallmarks.展开更多
文摘我院基于国际麻醉护理联盟(International Federation of Nurse Anesthetists,简称IFNA)教育培训基地,从麻醉专科护士培训基地的申请、学员资质审核、带教教师资质与审核、培训课程设置、考核内容与方式设定及培训后效果评价等方面,建设了南京地区麻醉护理专科护士培训基地。本文就这方面的基地建设和体会进行总结并为麻醉护理专科护士培训提供参考。
文摘Dendritic cell (DC) maturation approved to be a pivotal process for initiating immunity. Many protocols were established in order the isolated peripheral blood mononuclear cells (PBMCs) from healthy donors to mature into dendritic cells (DCs). The purpose of this study was to present an effective and reliable DC maturation procedure by comparing three different protocols (Interleukin-4/Tumor Necrosis Factor-appha (IL-4/TNFa) DC protocol, Interferon alpha (IFNa) DC protocol and FAST DC protocol). Whole blood was collected from six healthy donors and PBMCs were isolated by Ficoll gradient centrifugation. The counted cells were incubated with the addition of three different cocktails of supplements for appropriate time period. The final mature DC population was examined either by its phenotypic characteristics under light microscope or by measuring the expression of antigen presenting molecules such as CD80 and CD86 by flow cytometry. It was found that the mature DCs, generated from the IL-4/TNFa DC protocol, expressed higher levels of CD80 and CD86. Furthermore, they sharply exhibited their phenotypic hallmarks.
