[Objective] This study aimed to explore the effects of IGFs system on the growth of goose skeletal muscles. [Method] Quantitative fluorescence PCR technique was adopted to study the variety- and gender-specificity in ...[Objective] This study aimed to explore the effects of IGFs system on the growth of goose skeletal muscles. [Method] Quantitative fluorescence PCR technique was adopted to study the variety- and gender-specificity in the expression of GHR, IGF-I, IGF-IR and IGFBP-3 genes in leg muscles of 70-day-old Taihu goose and Wanxi white goose, and the correlation between their expression and carcass traits was also investigated. [Resull] There was no variety difference in the expression of GHR, IGF-t, IGF-IR and IGFBP-3 genes in leg muscles of Taihu goose and Wanxi white goose, but there were significant variety differences in the body weight and leg muscle weight. There were no gender difference in the body weight, leg muscle weight and the rate of leg muscles; except IGF-I mRNA level that was significantly higher in male Taihu goose than in female ones（P=0.032）, there was no gender dif- ference in the expression of other three genes. Among the four tested genes, only IGFBP-3 mRNA exhibited an extremely significantly positively correlation with the rate of leg muscles, suggesting that IGFs may play a role in regulating the growth of leg muscles via IGFBP-3 system in 70-day-old goose. [Conclusion] This study provides theoretical basis for research in the skeletal growth and development.展开更多
The objective of this study was to determine the genotype effects of the bovine insulin-like growth factor Ⅰ （IGF-Ⅰ） and its binding protein 3 （IGFBP3） genes on growth and development traits in beef cows, includ...The objective of this study was to determine the genotype effects of the bovine insulin-like growth factor Ⅰ （IGF-Ⅰ） and its binding protein 3 （IGFBP3） genes on growth and development traits in beef cows, including 130 Chinese Simmental, 42 Nanyang, and 47 Luxi Yellow cattle. Sequence variations in the bovine IGF-Ⅰ and IGFBP3 genes were investigated by single strand conformation polymorphism （SSCP）. SSCPs were detected in 6 fragments, which is the 5＇-flanking region, the 2nd exon, the 5th exon, and the 5th intron of the IGF-1 gene, and the 2nd exon, the 3rd exon of the 1GFBP3 gene. Two polymorphisms, an A-to-G transition in the 2rid exon of the IGF-Ⅰ gene and a T-to-C transition in the 2rid exon of IGFBP3 gene were detected in 3 breeds. The allele frequencies of 2 polymorphisms were 0.0411 （A）, 0.9589 （B）, and 0.7237 （A）, 0.2763 （B）, respectively. These 2 loci were analyzed to associate with body weight, height at withers, body length, heart girth, rump width, and beef production index （BPI） at 0, 6, 12, 24, and 36-month old. The IGFBP3 locus was shown to be associated with rump width, heart girth at 24-month and 36-month. Animals with BB genotype had higher rump width （24.86 ± 0.47） cm at 24-month and （27.50 ± 0.63） em at 36-month. The heart girth was highest for the individuals with BB genotype （171.33 ± 1.84） cm and higher than those with AB genotype （166.68 ± 1.13） cm （P〈 0.05） at 36-month.展开更多
Objective To investigate the effects of combination adenovirusmediated HSVtk/GCV system and antisense IGF1 gene therapy for rat glioma and analyze the mechanism.Methods Using the recombinant adenovirus vector,GCV kill...Objective To investigate the effects of combination adenovirusmediated HSVtk/GCV system and antisense IGF1 gene therapy for rat glioma and analyze the mechanism.Methods Using the recombinant adenovirus vector,GCV killing effeciency after combined gene transfer of HSVtk and antisense IGF1 was observed in vitro.Rat glioma was treated with HSVtk/GCV and antisense IGF1 and the survival rate of rats was observed.Results C6 cells transfected with tk and antisense IGF1 gene were more sensitive to GCV than that transfected with tk gene alone.The survival of the combination gene therapy group was prolonged significantly and large amounts of CD+ 4,CD+ 8 lymphocytes were detected in the tumor tissues.Conclusion Antisense IGF1 gene may enhance the tumorkilling effects of HSVtk/GCV.展开更多
目的:观察消痰通腑方对小鼠结肠癌CT26盲肠原位移植瘤肝转移的影响及肝组织中胰岛素生长因子IGF-I/IR表达的影响,探讨消痰通腑方干预结肠癌肝转移的作用机制。方法:将造模成功的小鼠随机分为模型组、中药组,另设正常组,每组10只,中药组...目的:观察消痰通腑方对小鼠结肠癌CT26盲肠原位移植瘤肝转移的影响及肝组织中胰岛素生长因子IGF-I/IR表达的影响,探讨消痰通腑方干预结肠癌肝转移的作用机制。方法:将造模成功的小鼠随机分为模型组、中药组,另设正常组,每组10只,中药组给予消痰通腑方灌胃,每日2次。待模型组小鼠成恶病质状态时,处死小鼠,取瘤组织及肝组织,观察各组荷瘤鼠肿瘤生长情况及抑瘤率;并运用免疫组化及实时荧光定量聚合酶链式反应(real time PCR)观察各组荷瘤鼠结肠癌组织IGF-I/IGF-IR蛋白及mRNA表达。结果:中药组小鼠瘤重、肝重、肝脏指数均低于模型组(P<0.05),与空白组比较,差异无统计学意义(P>0.05),中药组小鼠肝组织中IGF-I/IGF-IR蛋白及mRNA的表达均低于模型组(P<0.05),与空白组比较,差异无统计学意义(P>0.05)。结论:消痰通腑方可明显抑制小鼠结肠癌CT26原位移植瘤的生长,且能抑制肝脏转移,下调结肠癌肝转移组织中IGF-I/IGF-IR蛋白及mRNA的表达,可能是其抗结肠癌肝转移的机制之一。展开更多
基金Supported by the National Sci-tech Support Plan(2012BAD39B04)the Special Fund for Yangzhou Municipal Key Agricultural Science and Technology Projects(YZ2011067)Special Fund for the Industrial Technology System Construction of Modern Agriculture(nycytx-42-G1)~~
文摘[Objective] This study aimed to explore the effects of IGFs system on the growth of goose skeletal muscles. [Method] Quantitative fluorescence PCR technique was adopted to study the variety- and gender-specificity in the expression of GHR, IGF-I, IGF-IR and IGFBP-3 genes in leg muscles of 70-day-old Taihu goose and Wanxi white goose, and the correlation between their expression and carcass traits was also investigated. [Resull] There was no variety difference in the expression of GHR, IGF-t, IGF-IR and IGFBP-3 genes in leg muscles of Taihu goose and Wanxi white goose, but there were significant variety differences in the body weight and leg muscle weight. There were no gender difference in the body weight, leg muscle weight and the rate of leg muscles; except IGF-I mRNA level that was significantly higher in male Taihu goose than in female ones（P=0.032）, there was no gender dif- ference in the expression of other three genes. Among the four tested genes, only IGFBP-3 mRNA exhibited an extremely significantly positively correlation with the rate of leg muscles, suggesting that IGFs may play a role in regulating the growth of leg muscles via IGFBP-3 system in 70-day-old goose. [Conclusion] This study provides theoretical basis for research in the skeletal growth and development.
基金funded by the National Key Technology R&D Program of the China (2006BAD01A10,2006BAD14B07, 2006BAD04A16)the National High Technology Research and Development Program of China (2006AA10Z197)
文摘The objective of this study was to determine the genotype effects of the bovine insulin-like growth factor Ⅰ （IGF-Ⅰ） and its binding protein 3 （IGFBP3） genes on growth and development traits in beef cows, including 130 Chinese Simmental, 42 Nanyang, and 47 Luxi Yellow cattle. Sequence variations in the bovine IGF-Ⅰ and IGFBP3 genes were investigated by single strand conformation polymorphism （SSCP）. SSCPs were detected in 6 fragments, which is the 5＇-flanking region, the 2nd exon, the 5th exon, and the 5th intron of the IGF-1 gene, and the 2nd exon, the 3rd exon of the 1GFBP3 gene. Two polymorphisms, an A-to-G transition in the 2rid exon of the IGF-Ⅰ gene and a T-to-C transition in the 2rid exon of IGFBP3 gene were detected in 3 breeds. The allele frequencies of 2 polymorphisms were 0.0411 （A）, 0.9589 （B）, and 0.7237 （A）, 0.2763 （B）, respectively. These 2 loci were analyzed to associate with body weight, height at withers, body length, heart girth, rump width, and beef production index （BPI） at 0, 6, 12, 24, and 36-month old. The IGFBP3 locus was shown to be associated with rump width, heart girth at 24-month and 36-month. Animals with BB genotype had higher rump width （24.86 ± 0.47） cm at 24-month and （27.50 ± 0.63） em at 36-month. The heart girth was highest for the individuals with BB genotype （171.33 ± 1.84） cm and higher than those with AB genotype （166.68 ± 1.13） cm （P〈 0.05） at 36-month.
文摘Objective To investigate the effects of combination adenovirusmediated HSVtk/GCV system and antisense IGF1 gene therapy for rat glioma and analyze the mechanism.Methods Using the recombinant adenovirus vector,GCV killing effeciency after combined gene transfer of HSVtk and antisense IGF1 was observed in vitro.Rat glioma was treated with HSVtk/GCV and antisense IGF1 and the survival rate of rats was observed.Results C6 cells transfected with tk and antisense IGF1 gene were more sensitive to GCV than that transfected with tk gene alone.The survival of the combination gene therapy group was prolonged significantly and large amounts of CD+ 4,CD+ 8 lymphocytes were detected in the tumor tissues.Conclusion Antisense IGF1 gene may enhance the tumorkilling effects of HSVtk/GCV.
文摘目的:观察消痰通腑方对小鼠结肠癌CT26盲肠原位移植瘤肝转移的影响及肝组织中胰岛素生长因子IGF-I/IR表达的影响,探讨消痰通腑方干预结肠癌肝转移的作用机制。方法:将造模成功的小鼠随机分为模型组、中药组,另设正常组,每组10只,中药组给予消痰通腑方灌胃,每日2次。待模型组小鼠成恶病质状态时,处死小鼠,取瘤组织及肝组织,观察各组荷瘤鼠肿瘤生长情况及抑瘤率;并运用免疫组化及实时荧光定量聚合酶链式反应(real time PCR)观察各组荷瘤鼠结肠癌组织IGF-I/IGF-IR蛋白及mRNA表达。结果:中药组小鼠瘤重、肝重、肝脏指数均低于模型组(P<0.05),与空白组比较,差异无统计学意义(P>0.05),中药组小鼠肝组织中IGF-I/IGF-IR蛋白及mRNA的表达均低于模型组(P<0.05),与空白组比较,差异无统计学意义(P>0.05)。结论:消痰通腑方可明显抑制小鼠结肠癌CT26原位移植瘤的生长,且能抑制肝脏转移,下调结肠癌肝转移组织中IGF-I/IGF-IR蛋白及mRNA的表达,可能是其抗结肠癌肝转移的机制之一。
