[Objective] To explore the polymorphism of the 3′ flank region of equine IGF-Ⅰ gene. [Method] The 3′ flank region sequences of IGF-Ⅰ gene were amplified from genomic DNA of 270 horses, which included 4 types of Mo...[Objective] To explore the polymorphism of the 3′ flank region of equine IGF-Ⅰ gene. [Method] The 3′ flank region sequences of IGF-Ⅰ gene were amplified from genomic DNA of 270 horses, which included 4 types of Mongolian horse, Sanhe horse and Thoroughbred, and then analyzed by PCR-SSCP. [Result] Three genotypes (AA, BB and AB) were detected by PCR-SSCP and the distribution of genotypes of all research objects except Xinihe horse and Baerhu horse were in line with the "Hardy-Weinberg Law". [Conclusion] There was a polymorphic locus in the 3′ flank region of IGF-Ⅰ gene, which might affect the equine growth and development mechanism. The study is of important theoretical and practical significance to improve the performance and to develop equine industry.展开更多
目的:探讨芡实对糖尿病肾病(diebetic nephrothy,DN)大鼠肾组织中细胞因子信号抑制因子-3(suppressor of cytokine signaling-3,SOCS-3)及胰岛素样生长因子-1(insulin like growth factor-1,IGF-1)表达的影响及可能机制。方法:采用单剂...目的:探讨芡实对糖尿病肾病(diebetic nephrothy,DN)大鼠肾组织中细胞因子信号抑制因子-3(suppressor of cytokine signaling-3,SOCS-3)及胰岛素样生长因子-1(insulin like growth factor-1,IGF-1)表达的影响及可能机制。方法:采用单剂量腹腔注射链脲佐菌素(STZ,45 mg/kg)建立大鼠DN模型,将DN模型大鼠随机分为5组:DN组(DN组)、小剂量芡实组(EL,1.5 g·kg-1·d-1)、中剂量芡实组(EM,3.0 g·kg·-1d-1)、大剂量芡实组(EH,6.0 g·kg-1·d-1)及氯沙坦钾组(LP,30 mg·kg-1·d-1);另设正常对照组(NC组),每组10只。分别治疗12周后测定各组大鼠生化指标;HE、Masson、PAS染色及电镜观察肾组织病理变化;免疫组化、Western blot法检测肾组织中SOCS-3与IGF-1表达情况。结果:12周末,与NC组相比,DN组大鼠24 h尿蛋白定量(24 h Upr)、尿素氮(BUN)及血肌酐(Scr)均明显升高(P<0.05),肾小球体积增大、系膜细胞增生、基质增多,肾小管扩张,肾组织中SOCS-3表达增加、IGF-1表达明显增加(P<0.05);与DN组相比,LP组与EM组大鼠24 h Upr、BUN及Scr明显降低(P<0.05),病理改变减轻,肾组织SOCS-3表达明显增加、IGF-1表达明显下降(P<0.05);LP组与EM组间降尿蛋白作用差异无统计学意义。结论:芡实可能通过上调SOCS-3表达,抑制IGF-1过表达进而减少蛋白尿,发挥肾脏保护作用。展开更多
文摘[Objective] To explore the polymorphism of the 3′ flank region of equine IGF-Ⅰ gene. [Method] The 3′ flank region sequences of IGF-Ⅰ gene were amplified from genomic DNA of 270 horses, which included 4 types of Mongolian horse, Sanhe horse and Thoroughbred, and then analyzed by PCR-SSCP. [Result] Three genotypes (AA, BB and AB) were detected by PCR-SSCP and the distribution of genotypes of all research objects except Xinihe horse and Baerhu horse were in line with the "Hardy-Weinberg Law". [Conclusion] There was a polymorphic locus in the 3′ flank region of IGF-Ⅰ gene, which might affect the equine growth and development mechanism. The study is of important theoretical and practical significance to improve the performance and to develop equine industry.
文摘目的:探讨芡实对糖尿病肾病(diebetic nephrothy,DN)大鼠肾组织中细胞因子信号抑制因子-3(suppressor of cytokine signaling-3,SOCS-3)及胰岛素样生长因子-1(insulin like growth factor-1,IGF-1)表达的影响及可能机制。方法:采用单剂量腹腔注射链脲佐菌素(STZ,45 mg/kg)建立大鼠DN模型,将DN模型大鼠随机分为5组:DN组(DN组)、小剂量芡实组(EL,1.5 g·kg-1·d-1)、中剂量芡实组(EM,3.0 g·kg·-1d-1)、大剂量芡实组(EH,6.0 g·kg-1·d-1)及氯沙坦钾组(LP,30 mg·kg-1·d-1);另设正常对照组(NC组),每组10只。分别治疗12周后测定各组大鼠生化指标;HE、Masson、PAS染色及电镜观察肾组织病理变化;免疫组化、Western blot法检测肾组织中SOCS-3与IGF-1表达情况。结果:12周末,与NC组相比,DN组大鼠24 h尿蛋白定量(24 h Upr)、尿素氮(BUN)及血肌酐(Scr)均明显升高(P<0.05),肾小球体积增大、系膜细胞增生、基质增多,肾小管扩张,肾组织中SOCS-3表达增加、IGF-1表达明显增加(P<0.05);与DN组相比,LP组与EM组大鼠24 h Upr、BUN及Scr明显降低(P<0.05),病理改变减轻,肾组织SOCS-3表达明显增加、IGF-1表达明显下降(P<0.05);LP组与EM组间降尿蛋白作用差异无统计学意义。结论:芡实可能通过上调SOCS-3表达,抑制IGF-1过表达进而减少蛋白尿,发挥肾脏保护作用。
