Insulin-like growth factor Ⅱhas profound effects on the growth and differentiation of animal embryo. Some researches indicated that it affects the fat metabolism of poultry. This study was designed to investigate t...Insulin-like growth factor Ⅱhas profound effects on the growth and differentiation of animal embryo. Some researches indicated that it affects the fat metabolism of poultry. This study was designed to investigate the effect of IGF2 on chicken fatness traits. Broiler, Hyline Brown layer and three native breeds (Shiqiza, Beijing You, Baier) were used in this research. Body weight and body composition traits were measured in broiler line at the age of 7 weeks. Primers for exon2 in IGF2 were designed from database of chicken genomic sequence. Polymorphisms were detected by PCR-SSCP and DNA sequencing. The total χ2 test results showed that there was a significant difference (P < 0.01) in the frequency of genotype among breeds. A C/G mutation at base position 139 was found among individuals in broiler line and the least square analysis showed that BB genotype birds had significant lower (P <0.05) abdominal fat weight and percentage of abdominal fat than AA or AB genotype birds. From the results we can conclude putatively that IGF2 gene is the major gene affecting the fatness traits of chicken or it links with the major gene, and the mutation could be used as the molecular genetic marker to select the chicken for low abdominal fat.展开更多
Fattiness is an important parameter to estimate meat quality, which has high heritability. In this experiment, F2 chickens derived from Broilers crossing to Silky were used to study the effect of extracellular fatty a...Fattiness is an important parameter to estimate meat quality, which has high heritability. In this experiment, F2 chickens derived from Broilers crossing to Silky were used to study the effect of extracellular fatty acid binding protein (EX-FABP) gene on abdominal fat accumulation. 1.6 kb of the 5′ region of the gene was amplified by six pairs of primers, and then single nucleotide poly-morphisms (SNPs) were detected by the technique of single strand conformation polymorphism (SSCP) and then confirmed by sequencing. There were four nucleotides variations found, A-G at -1807, C-A at -1805, T-C at -1011 and a C insertion at -1000 respectively. The result of least square analysis suggests that the birds with BB genotype defined by the second pair of primer have a higher abdominal fat weight and abdominal fat percentage than the birds with the other genotypes (AA and AB). It implied that EX-FABP gene could be a candidate locus or linked to a major gene to significantly affect abdominal fat traits in chicken.展开更多
Myostatin, a new member of the TGF-p superfamily, is predominantly expressed in skeletal muscle cells and functions as a negative regulator of skeletal muscle growth in animals. Recently, we have reported three single...Myostatin, a new member of the TGF-p superfamily, is predominantly expressed in skeletal muscle cells and functions as a negative regulator of skeletal muscle growth in animals. Recently, we have reported three single nucleotide polymorphisms (SNPs) in the chicken my-ostatin gene. Herein, we investigate the association of those SNPs with the production traits in a F2 chicken line derived from Broilers crossing to Silky with the least square analysis. The results show that the BB and AA genotypes are strongly associated with abdominal fat weight (AFW), abdominal fat percentage (AFP), and birth weight (BW) (P < 0.05). Breast muscle percentage (BMP) of the AA type is higher than that of the AB type. The breast muscle weight and breast muscle percentages of F2 individuals have significant difference between CC and DD genotypes (P< 0.05). Breast muscle weight (BMW) of EF birds is higher than that of EE birds (P< 0.05). In this report, we present the first genetic evidence to show that chicken myostatin not only plays an important role in controlling skeletal muscle growth and differentiation, but also may be involved in regulation of adipose growth in chicken.展开更多
为探讨HMG box蛋白1(HMG box protein 1,HBP1)基因启动子区多态性对鸡腹脂率的影响,以东北农业大学高、低脂双向选择品系第十九世代肉鸡为试验材料,采用直接测序法检测HBP1基因多态性和个体基因型,共获得168个SNPs位点基因型。应用滑动...为探讨HMG box蛋白1(HMG box protein 1,HBP1)基因启动子区多态性对鸡腹脂率的影响,以东北农业大学高、低脂双向选择品系第十九世代肉鸡为试验材料,采用直接测序法检测HBP1基因多态性和个体基因型,共获得168个SNPs位点基因型。应用滑动窗口法对位于该基因启动子区54个多态性位点构建单倍型,分别开展单位点和单倍型与鸡腹脂率的关联分析。结果表明,HBP1基因启动子区有7个(g.-5335G>A、g.-4354T>A、g.-3328C>T、g.-3327G>A、g.-1013T>C、g.-990G>A和g.-159A>C)位点显著影响肉鸡腹脂率(P<0.05)。此外,等位基因频率分析显示,这些位点等位基因频率在两系间分布存在极显著差异(P<0.0001)。研究结果表明,HBP1基因为影响鸡腹脂率重要候选基因,7个多态性位点为影响鸡腹脂率重要分子标记。展开更多
基金This study was funded by the National 863 Program of China(2002AA211021)Hei long-j iang Province Outstanding Youth Foundation,China(JC-02-06).
文摘Insulin-like growth factor Ⅱhas profound effects on the growth and differentiation of animal embryo. Some researches indicated that it affects the fat metabolism of poultry. This study was designed to investigate the effect of IGF2 on chicken fatness traits. Broiler, Hyline Brown layer and three native breeds (Shiqiza, Beijing You, Baier) were used in this research. Body weight and body composition traits were measured in broiler line at the age of 7 weeks. Primers for exon2 in IGF2 were designed from database of chicken genomic sequence. Polymorphisms were detected by PCR-SSCP and DNA sequencing. The total χ2 test results showed that there was a significant difference (P < 0.01) in the frequency of genotype among breeds. A C/G mutation at base position 139 was found among individuals in broiler line and the least square analysis showed that BB genotype birds had significant lower (P <0.05) abdominal fat weight and percentage of abdominal fat than AA or AB genotype birds. From the results we can conclude putatively that IGF2 gene is the major gene affecting the fatness traits of chicken or it links with the major gene, and the mutation could be used as the molecular genetic marker to select the chicken for low abdominal fat.
基金the National Basic Research Development Program and National Natural Science Foundation of China (Grant No. 39725022).
文摘Fattiness is an important parameter to estimate meat quality, which has high heritability. In this experiment, F2 chickens derived from Broilers crossing to Silky were used to study the effect of extracellular fatty acid binding protein (EX-FABP) gene on abdominal fat accumulation. 1.6 kb of the 5′ region of the gene was amplified by six pairs of primers, and then single nucleotide poly-morphisms (SNPs) were detected by the technique of single strand conformation polymorphism (SSCP) and then confirmed by sequencing. There were four nucleotides variations found, A-G at -1807, C-A at -1805, T-C at -1011 and a C insertion at -1000 respectively. The result of least square analysis suggests that the birds with BB genotype defined by the second pair of primer have a higher abdominal fat weight and abdominal fat percentage than the birds with the other genotypes (AA and AB). It implied that EX-FABP gene could be a candidate locus or linked to a major gene to significantly affect abdominal fat traits in chicken.
基金supported by the Chinese National Foundation to Outstanding Young ScientistsNational Natural Science Foundation of China(Grant No.30170462)National High-Tech Project.
文摘Myostatin, a new member of the TGF-p superfamily, is predominantly expressed in skeletal muscle cells and functions as a negative regulator of skeletal muscle growth in animals. Recently, we have reported three single nucleotide polymorphisms (SNPs) in the chicken my-ostatin gene. Herein, we investigate the association of those SNPs with the production traits in a F2 chicken line derived from Broilers crossing to Silky with the least square analysis. The results show that the BB and AA genotypes are strongly associated with abdominal fat weight (AFW), abdominal fat percentage (AFP), and birth weight (BW) (P < 0.05). Breast muscle percentage (BMP) of the AA type is higher than that of the AB type. The breast muscle weight and breast muscle percentages of F2 individuals have significant difference between CC and DD genotypes (P< 0.05). Breast muscle weight (BMW) of EF birds is higher than that of EE birds (P< 0.05). In this report, we present the first genetic evidence to show that chicken myostatin not only plays an important role in controlling skeletal muscle growth and differentiation, but also may be involved in regulation of adipose growth in chicken.
基金supported by the State Major Basic Research Development Program(Grant No.G20000161)the National Natural Science Foundation of China(Grant No.39725022)Beijing Natural Science Foundation(Grant No.5981001)
基金This work was supported by a grant from the National Natural Science Foundation Key Project (No. 30430510), the Excellent Young Teachers Program of MOE. P. R. C (No.1985), and Program for New Century Excellent Talents in University (No. NCET-04-0343).
文摘为探讨HMG box蛋白1(HMG box protein 1,HBP1)基因启动子区多态性对鸡腹脂率的影响,以东北农业大学高、低脂双向选择品系第十九世代肉鸡为试验材料,采用直接测序法检测HBP1基因多态性和个体基因型,共获得168个SNPs位点基因型。应用滑动窗口法对位于该基因启动子区54个多态性位点构建单倍型,分别开展单位点和单倍型与鸡腹脂率的关联分析。结果表明,HBP1基因启动子区有7个(g.-5335G>A、g.-4354T>A、g.-3328C>T、g.-3327G>A、g.-1013T>C、g.-990G>A和g.-159A>C)位点显著影响肉鸡腹脂率(P<0.05)。此外,等位基因频率分析显示,这些位点等位基因频率在两系间分布存在极显著差异(P<0.0001)。研究结果表明,HBP1基因为影响鸡腹脂率重要候选基因,7个多态性位点为影响鸡腹脂率重要分子标记。
