In this study, we characterized and quantified vitamin B12 in canned apple snails, escargots, (boiled plain) using a microbiological assay based on Lactobacillus delbrueckii ATCC 7830. Vitamin B12 contents of canned e...In this study, we characterized and quantified vitamin B12 in canned apple snails, escargots, (boiled plain) using a microbiological assay based on Lactobacillus delbrueckii ATCC 7830. Vitamin B12 contents of canned escargots (boiled plain) were varied from approximately 0.8 μg/100g weight to approximately 5.5 μg/100g weight (mean values, 2.2 μg/100g weight). We identified vitamin B12 compounds from escargots using liquid chromatography-electrospray ionization/tandem mass spectrometry. We found that escargots contained true vitamin B12 and two inactive corrinoids, which were identified as factor IIIm (or methoxymensimidazolyl cyanocobamide), and factor S (or 2-methylmercaptoadenyl cyanocobamide). These results indicate that canned escargots (boiled plain) are not good sources of vitamin B12 for humans.展开更多
文摘In this study, we characterized and quantified vitamin B12 in canned apple snails, escargots, (boiled plain) using a microbiological assay based on Lactobacillus delbrueckii ATCC 7830. Vitamin B12 contents of canned escargots (boiled plain) were varied from approximately 0.8 μg/100g weight to approximately 5.5 μg/100g weight (mean values, 2.2 μg/100g weight). We identified vitamin B12 compounds from escargots using liquid chromatography-electrospray ionization/tandem mass spectrometry. We found that escargots contained true vitamin B12 and two inactive corrinoids, which were identified as factor IIIm (or methoxymensimidazolyl cyanocobamide), and factor S (or 2-methylmercaptoadenyl cyanocobamide). These results indicate that canned escargots (boiled plain) are not good sources of vitamin B12 for humans.