从广东省南澳县西山等养蛙场多次收集患黄杆菌病牛蛙,其症状是:歪头、狂游、双眼白内障和腹水等。从病蛙脑浆、眼底和腹水中分离到多株病原菌,急性毒性试验表明病原菌对健康牛蛙致病力强。取有代表性的981007-2等菌株进行系统的生理生...从广东省南澳县西山等养蛙场多次收集患黄杆菌病牛蛙,其症状是:歪头、狂游、双眼白内障和腹水等。从病蛙脑浆、眼底和腹水中分离到多株病原菌,急性毒性试验表明病原菌对健康牛蛙致病力强。取有代表性的981007-2等菌株进行系统的生理生化试验显示,革兰氏染色阴性、无鞭毛、无芽孢的短杆菌;分离菌株内毒素检验阳性;病原菌显示了对青霉素类药物高度耐药、好氧型、利用葡萄糖等生理、生化特性。经VITEK-AMS-60自动化检测系统鉴定为黄杆菌IIb群(Plavobacterium group IIb)。选用47种抗菌药物,通过K-B纸片扩散法对病原菌进行耐药性研究结果,分离菌株对38种抗菌药物显示耐药(占80.85％)、对4种药物中等敏感(占8.51％),仅对利福平、环丙沙星、氧氟沙星、氟啶酸、麦迪霉素等5种药物(占10.64％)敏感。应用试管稀释法测定上述5种敏感药物的平均MIC分别是0.19、0.27、0.53、2.40和10.2μg/ml。展开更多
Objectives: To investigate the relation between metastatic potential of salivary adenoid cystic carcinoma (SACC) and tumor cell-platelet adhesion, and the antimetastatic effect of integrin IIb/IIIa inhibitor on SACC. ...Objectives: To investigate the relation between metastatic potential of salivary adenoid cystic carcinoma (SACC) and tumor cell-platelet adhesion, and the antimetastatic effect of integrin IIb/IIIa inhibitor on SACC. Methods: Tumor cell-platelet adhesion of highly metastatic SACC-LM, non-highly metastatic SACC-83 and effect of aspirin, arginine-aspartate (RD), magnesium acetylsalicylate on adhesion were studiedin vitro. Antimetastafic effect of aspirin, RD, magnesium acetysalicylate on experimental metastasis of SACC was observedin vivo. Results: The tumor cell-platelet adhesion was stronger in SACC-LM than in SACC-83. Aspirin, RD and magnesium acetylsalicylate could inhibit the adhesion of SACC-LM at the concentration of 1, 5 and 25 μg/ml. RD can inhibit experimental metastasis of SACC. Conclusion: Metastasis of SACC is related to platelet-tumor cell adhesion, RD could inhibit metastasis of SACC.展开更多
High-quality p-type boron-doped IIb diamond large single crystals are successfully synthesized by the temperature gradient method in a china-type cubic anvil high-pressure apparatus at about 5.5 GPa and 1600 K. The mo...High-quality p-type boron-doped IIb diamond large single crystals are successfully synthesized by the temperature gradient method in a china-type cubic anvil high-pressure apparatus at about 5.5 GPa and 1600 K. The morphologies and surface textures of the synthetic diamond crystals with different boron additive quantities are characterized by using an optical microscope and a scanning electron microscope respectively. The impurities of nitrogen and boron in diamonds are detected by micro Fourier transform infrared technique. The electrical properties including resistivities, Hall coefficients, Hall mobilities and carrier densities of the synthesized samples are measured by a four-point probe and the Hall effect method. The results show that large p-type boron-doped diamond single crystals with few nitrogen impurities have been synthesized. With the increase of quantity of additive boron, some high-index crystal faces such as {113} gradually disappear, and some stripes and triangle pits occur on the crystal surface. This work is helpful for the further research and application of boron-doped semiconductor diamond.展开更多
文摘从广东省南澳县西山等养蛙场多次收集患黄杆菌病牛蛙,其症状是:歪头、狂游、双眼白内障和腹水等。从病蛙脑浆、眼底和腹水中分离到多株病原菌,急性毒性试验表明病原菌对健康牛蛙致病力强。取有代表性的981007-2等菌株进行系统的生理生化试验显示,革兰氏染色阴性、无鞭毛、无芽孢的短杆菌;分离菌株内毒素检验阳性;病原菌显示了对青霉素类药物高度耐药、好氧型、利用葡萄糖等生理、生化特性。经VITEK-AMS-60自动化检测系统鉴定为黄杆菌IIb群(Plavobacterium group IIb)。选用47种抗菌药物,通过K-B纸片扩散法对病原菌进行耐药性研究结果,分离菌株对38种抗菌药物显示耐药(占80.85％)、对4种药物中等敏感(占8.51％),仅对利福平、环丙沙星、氧氟沙星、氟啶酸、麦迪霉素等5种药物(占10.64％)敏感。应用试管稀释法测定上述5种敏感药物的平均MIC分别是0.19、0.27、0.53、2.40和10.2μg/ml。
基金the National Natural Science Foundation of China (No. 39270723).
文摘Objectives: To investigate the relation between metastatic potential of salivary adenoid cystic carcinoma (SACC) and tumor cell-platelet adhesion, and the antimetastatic effect of integrin IIb/IIIa inhibitor on SACC. Methods: Tumor cell-platelet adhesion of highly metastatic SACC-LM, non-highly metastatic SACC-83 and effect of aspirin, arginine-aspartate (RD), magnesium acetylsalicylate on adhesion were studiedin vitro. Antimetastafic effect of aspirin, RD, magnesium acetysalicylate on experimental metastasis of SACC was observedin vivo. Results: The tumor cell-platelet adhesion was stronger in SACC-LM than in SACC-83. Aspirin, RD and magnesium acetylsalicylate could inhibit the adhesion of SACC-LM at the concentration of 1, 5 and 25 μg/ml. RD can inhibit experimental metastasis of SACC. Conclusion: Metastasis of SACC is related to platelet-tumor cell adhesion, RD could inhibit metastasis of SACC.
基金supported by the Young Scientists Fund of the National Natural Science Foundation of China (Grant No. 51001042)the Doctor Foundation of the Henan Polytechnic University,China (Grant No. 2010-32)
文摘High-quality p-type boron-doped IIb diamond large single crystals are successfully synthesized by the temperature gradient method in a china-type cubic anvil high-pressure apparatus at about 5.5 GPa and 1600 K. The morphologies and surface textures of the synthetic diamond crystals with different boron additive quantities are characterized by using an optical microscope and a scanning electron microscope respectively. The impurities of nitrogen and boron in diamonds are detected by micro Fourier transform infrared technique. The electrical properties including resistivities, Hall coefficients, Hall mobilities and carrier densities of the synthesized samples are measured by a four-point probe and the Hall effect method. The results show that large p-type boron-doped diamond single crystals with few nitrogen impurities have been synthesized. With the increase of quantity of additive boron, some high-index crystal faces such as {113} gradually disappear, and some stripes and triangle pits occur on the crystal surface. This work is helpful for the further research and application of boron-doped semiconductor diamond.
