Objective. An association between polymorphism in the gene coding for the anti- inflammatory cytokine interleukin- 1- receptor antagonist (IL- 1Ra) and ulcerative colitis (UC) has been reported. To date, there is no r...Objective. An association between polymorphism in the gene coding for the anti- inflammatory cytokine interleukin- 1- receptor antagonist (IL- 1Ra) and ulcerative colitis (UC) has been reported. To date, there is no report from India confirming this association. In the present study the aim was to assess the allele frequencies and carriage rates of different alleles of 86 bp (base pair) variable number tandem repeat (VNTR) in intron 2 of the IL- 1Ra gene in patients with inflammatory bowel disease (IBD) and healthy controls from northern India. Material and methods. Eighty- two patients with UC, 21 with Crohn’ s disease (CD) and 141 ethnically matched controls were enrolled in this study. Genotyping was done using a polymerase chain reaction (PCR) amplification of the intron- 2 fragment harboring a VNTR nucleotide sequence. The PCR products were separated on 2% agarose gel. Statistical analysis was performed using the chi- squared (χ 2) test. Results. The frequencies of allele 2 in UC, CD and healthy controls were 26% , 50% and 24% , respectively. The frequency of allele 2 in CD was higher than that in UC (p = 0.002; OR = 2.9) and healthy controls (p = 0.001; OR = 3.1; 95% CI = 1.5- 6.3). Alleles 3 and 4 were absent in patients with CD, while allele 5 was absent in all three groups. Conclusions. The present study demonstrated an association between allele 2 and patients with CD but not with UC. Interestingly, the allele frequency and carriage rates of allele 2 were significantly higher in patients with CD than in patients with UC and in healthy subjects. Ethnic differences, genetic heterogeneity and sample size could be the reasons for such differences in comparison with studies from the West.展开更多
Genotypes and allele distributions for the interleukin (IL)- 1β - 511 C/T and the IL- 1 receptor antagonist gene intron 2 tandem repeat polymorphisms were compared in 206 women with recurrent miscarriage and a contro...Genotypes and allele distributions for the interleukin (IL)- 1β - 511 C/T and the IL- 1 receptor antagonist gene intron 2 tandem repeat polymorphisms were compared in 206 women with recurrent miscarriage and a control population. No significant differences were observed between the distributions of IL- 1β or IL- 1 receptor antagonist gene alleles in either the recurrent miscarriage group as a whole or when divided according to the cause of recurrent miscarriage compared with controls, which suggests that variation in the IL- 1 receptor antagonist gene and IL- 1β genes individually does not play a role in susceptibility to recurrent miscarriage.展开更多
In a prospective case- control study we investigated the association of a common polymorphism of the interleukin 1 receptor antagonist gene (IL- 1 RA) with the occurrence and the clinical characteristics of polycystic...In a prospective case- control study we investigated the association of a common polymorphism of the interleukin 1 receptor antagonist gene (IL- 1 RA) with the occurrence and the clinical characteristics of polycystic ovary syndrome (PCOS). Allele frequencies did not vary statistically significantly among women with PCOS and healthy controls or within the PCOS group with respect to their clinical characteristics.展开更多
文摘Objective. An association between polymorphism in the gene coding for the anti- inflammatory cytokine interleukin- 1- receptor antagonist (IL- 1Ra) and ulcerative colitis (UC) has been reported. To date, there is no report from India confirming this association. In the present study the aim was to assess the allele frequencies and carriage rates of different alleles of 86 bp (base pair) variable number tandem repeat (VNTR) in intron 2 of the IL- 1Ra gene in patients with inflammatory bowel disease (IBD) and healthy controls from northern India. Material and methods. Eighty- two patients with UC, 21 with Crohn’ s disease (CD) and 141 ethnically matched controls were enrolled in this study. Genotyping was done using a polymerase chain reaction (PCR) amplification of the intron- 2 fragment harboring a VNTR nucleotide sequence. The PCR products were separated on 2% agarose gel. Statistical analysis was performed using the chi- squared (χ 2) test. Results. The frequencies of allele 2 in UC, CD and healthy controls were 26% , 50% and 24% , respectively. The frequency of allele 2 in CD was higher than that in UC (p = 0.002; OR = 2.9) and healthy controls (p = 0.001; OR = 3.1; 95% CI = 1.5- 6.3). Alleles 3 and 4 were absent in patients with CD, while allele 5 was absent in all three groups. Conclusions. The present study demonstrated an association between allele 2 and patients with CD but not with UC. Interestingly, the allele frequency and carriage rates of allele 2 were significantly higher in patients with CD than in patients with UC and in healthy subjects. Ethnic differences, genetic heterogeneity and sample size could be the reasons for such differences in comparison with studies from the West.
文摘Genotypes and allele distributions for the interleukin (IL)- 1β - 511 C/T and the IL- 1 receptor antagonist gene intron 2 tandem repeat polymorphisms were compared in 206 women with recurrent miscarriage and a control population. No significant differences were observed between the distributions of IL- 1β or IL- 1 receptor antagonist gene alleles in either the recurrent miscarriage group as a whole or when divided according to the cause of recurrent miscarriage compared with controls, which suggests that variation in the IL- 1 receptor antagonist gene and IL- 1β genes individually does not play a role in susceptibility to recurrent miscarriage.
文摘In a prospective case- control study we investigated the association of a common polymorphism of the interleukin 1 receptor antagonist gene (IL- 1 RA) with the occurrence and the clinical characteristics of polycystic ovary syndrome (PCOS). Allele frequencies did not vary statistically significantly among women with PCOS and healthy controls or within the PCOS group with respect to their clinical characteristics.
