Within the interleukin-17 （IL-17） family of cytokines, IL-17A is known to be critical in the host defense against fungal infections; however, the function of the other IL-17 family members in anti-fungal immunity re...Within the interleukin-17 （IL-17） family of cytokines, IL-17A is known to be critical in the host defense against fungal infections; however, the function of the other IL-17 family members in anti-fungal immunity remains largely unknown. Here, we show that IL-17C expression was highly induced in kidney epithelial cells after fungal infection. Mice that lacked IL-17C exhibited increased survival and attenuated kidney tissue damage, although they had similar fungal loads. IL-17C deficiency resulted in decreased pro-inflammatory cytokine expression compared with wild-type control mice. Additionally, IL-17C directly acted on renal epithelial cells in vitro to promote pro-inflammatory cytokine production. Taken together, our data demonstrate that IL-17C is a critical factor that potentiates inflammatory responses and causes host injury during fungal infection.展开更多
微小RNA(microRNA,miRNA)是一种基因表达调控因子,本研究旨在研究miR-27b对IL-17诱导下的单核细胞趋化蛋白-1(MCP-1)表达的影响。我们转染miR-27bmimic于H9C2细胞中,采用SYBR green I荧光定量PCR检测H9C2心肌细胞中miR-27b和MCP-1基因...微小RNA(microRNA,miRNA)是一种基因表达调控因子,本研究旨在研究miR-27b对IL-17诱导下的单核细胞趋化蛋白-1(MCP-1)表达的影响。我们转染miR-27bmimic于H9C2细胞中,采用SYBR green I荧光定量PCR检测H9C2心肌细胞中miR-27b和MCP-1基因的表达情况,以ELISA分析H9C2细胞培养上清液中MCP-1蛋白的表达。在IL-17对MCP-1表达的影响的研究中,结果显示,IL-17作用2h后MCP-1mRNA的表达量开始升高,4h时达到高峰,尔后开始下降,而MCP-1蛋白的表达量0~24h逐渐升高。在miR-27b对IL-17诱导下MCP-1表达的影响的研究中,结果显示,IL-17组与空白对照组比较,MCP-1mRNA和蛋白水平显著升高(P<0.05);IL-17组与转染miRNA阴性对照+IL-17组比较,两组的MCP-1mRNA和蛋白水平无显著差异(P>0.05);转染miR-27bmimic+IL-17与转染miRNA阴性对照+IL-17组比较,MCP-1mRNA和蛋白水平显著下降(P<0.01)。本研究表明,(1)IL-17可上调MCP-1的表达,这与IL-17的作用时间有关。(2)过表达miR-27b可下调IL-17诱导下MCP-1的表达水平。展开更多
文摘目的:探讨维生素C(Vit C)对小鼠淋巴瘤EL4细胞诱导Treg/Th17失衡的影响。方法:采用CCK-8法检测不同浓度Vit C或泼尼松对小鼠淋巴瘤EL4细胞增殖的影响,选出对细胞增殖无明显抑制的浓度用于细胞干预。用转化生长因子(TGF)-β1、白细胞介素(IL)-6、IL-23诱导EL4细胞发生Treg/Th17平衡向Th17偏移,分别用Vit C、泼尼松干预,取细胞及培养上清,实时荧光定量PCR(qPCR)检测细胞中维甲酸相关孤核受体γt(RORγt)和叉头蛋白3(Foxp3)mRNA相对表达量,酶联免疫吸附试验(ELISA)法检测细胞培养上清液中IL-17A、IL-10水平。结果:0.2mmol/L Vit C、1μmol/L泼尼松对EL4细胞增殖无明显抑制作用,可使EL4细胞表达RORγt mRNA和Foxp3mRNA相对表达量升高,IL-10分泌减少,差异均有统计学意义(均P<0.05)。结论:0.2mmol/L Vit C对EL4细胞分化的Treg/Th17失衡有调节作用,效果与1μmol/L泼尼松类似,且作用强度与作用时间存在相关性。
文摘Within the interleukin-17 （IL-17） family of cytokines, IL-17A is known to be critical in the host defense against fungal infections; however, the function of the other IL-17 family members in anti-fungal immunity remains largely unknown. Here, we show that IL-17C expression was highly induced in kidney epithelial cells after fungal infection. Mice that lacked IL-17C exhibited increased survival and attenuated kidney tissue damage, although they had similar fungal loads. IL-17C deficiency resulted in decreased pro-inflammatory cytokine expression compared with wild-type control mice. Additionally, IL-17C directly acted on renal epithelial cells in vitro to promote pro-inflammatory cytokine production. Taken together, our data demonstrate that IL-17C is a critical factor that potentiates inflammatory responses and causes host injury during fungal infection.
文摘微小RNA(microRNA,miRNA)是一种基因表达调控因子,本研究旨在研究miR-27b对IL-17诱导下的单核细胞趋化蛋白-1(MCP-1)表达的影响。我们转染miR-27bmimic于H9C2细胞中,采用SYBR green I荧光定量PCR检测H9C2心肌细胞中miR-27b和MCP-1基因的表达情况,以ELISA分析H9C2细胞培养上清液中MCP-1蛋白的表达。在IL-17对MCP-1表达的影响的研究中,结果显示,IL-17作用2h后MCP-1mRNA的表达量开始升高,4h时达到高峰,尔后开始下降,而MCP-1蛋白的表达量0~24h逐渐升高。在miR-27b对IL-17诱导下MCP-1表达的影响的研究中,结果显示,IL-17组与空白对照组比较,MCP-1mRNA和蛋白水平显著升高(P<0.05);IL-17组与转染miRNA阴性对照+IL-17组比较,两组的MCP-1mRNA和蛋白水平无显著差异(P>0.05);转染miR-27bmimic+IL-17与转染miRNA阴性对照+IL-17组比较,MCP-1mRNA和蛋白水平显著下降(P<0.01)。本研究表明,(1)IL-17可上调MCP-1的表达,这与IL-17的作用时间有关。(2)过表达miR-27b可下调IL-17诱导下MCP-1的表达水平。
