Some poxvirus genome of vaccinia subgenus encoded IL-18 binding prolein gene,which could block IL-18 activity but no sequence similarity to membrane IL-18 receptors.The sheep poxvirus genome subgenus also had this gen...Some poxvirus genome of vaccinia subgenus encoded IL-18 binding prolein gene,which could block IL-18 activity but no sequence similarity to membrane IL-18 receptors.The sheep poxvirus genome subgenus also had this gene.A pair of primers spanning the ORF of goat IL-18 binding protein gene in sheep poxvirus genome were designed.This gene was cloned and expressed in E.coliwith pGEX-5X-3 plasmid.After being purified,the recombinant fusion protein could inhibit goat PBMC IFN- production induced by IL-18 in goat PBMC.展开更多
文摘Some poxvirus genome of vaccinia subgenus encoded IL-18 binding prolein gene,which could block IL-18 activity but no sequence similarity to membrane IL-18 receptors.The sheep poxvirus genome subgenus also had this gene.A pair of primers spanning the ORF of goat IL-18 binding protein gene in sheep poxvirus genome were designed.This gene was cloned and expressed in E.coliwith pGEX-5X-3 plasmid.After being purified,the recombinant fusion protein could inhibit goat PBMC IFN- production induced by IL-18 in goat PBMC.
