We assessed expression of IL-20 and its receptors in psoriasis,given the recent implication of IL-20 in epidermal hyperplasia.Psoriatic lesional(LS)skin consistently expressed more IL-20 mRNA than nonlesional(NL)skin....We assessed expression of IL-20 and its receptors in psoriasis,given the recent implication of IL-20 in epidermal hyperplasia.Psoriatic lesional(LS)skin consistently expressed more IL-20 mRNA than nonlesional(NL)skin.Immunoreactivity to IL-20 protein was greater in LS tissue and mainly localized to infiltrating CD68+/CD11c+(myeloid-derived)dermal leukocytes.Because this contrasted with earlier reports of a keratinocyte source,we assessed IL-20 mRNA expression in a variety of cells in vitro,and confirmed a myeloid-derived cellular source(monocytes).Plastic adhesion,activation of β2 integrins,and incubation with tumor necrosis factor-αstimulated expression in these cells.IL-20 receptor(IL-20R)αand IL-20RβmRNA was decreased in LS versus NL skin,which also contrasted with earlier findings.To investigate the relationship between IL-20 and disease activity,we examined psoriasis patients treated with the CD2-targeted agent alefacept.In therapeutic responders,lesional IL-20 mRNA decreased to NL levels,suggesting that CD2+leukocytes may proximally regulate IL-20.Finally,to assess IL-20 function,we used microarrays to screen IL-20-treated keratinocytes,which demonstrated upregulation of disease-related and IFN-γ-induced genes.Hence,IL-20 may influence inflammation through IFN-like effects.Together,these data indicate that IL-20 may be an important effector cytokine in psoriasis,and that its inhibition may represent a potential therapeutic target.展开更多
目的探讨血清CC趋化因子配体20(CCL20)及白介素-17(IL-17)水平与子痫前期的相关性及其对子痫前期的诊断效能。方法选择2021年1月至2022年12月陕西省人民医院产科收治的150例子痫前期孕妇为子痫前期组,另选取同期90例健康妊娠妇女为对照...目的探讨血清CC趋化因子配体20(CCL20)及白介素-17(IL-17)水平与子痫前期的相关性及其对子痫前期的诊断效能。方法选择2021年1月至2022年12月陕西省人民医院产科收治的150例子痫前期孕妇为子痫前期组,另选取同期90例健康妊娠妇女为对照组。采用酶联免疫吸附法(ELISA)检测血清CCL20及IL-17的表达水平,采用Pearson相关法分析血清CCL20及IL-17与子痫前期患者临床参数的相关性;采用受试者工作特征曲线(ROC)分析血清CCL20及IL-17对子痫前期的诊断价值。结果子痫前期组孕妇血清CCL20及IL-17水平均显著高于对照组(P<0.001)。子痫前期组孕妇血清CCL20及IL-17的表达均与收缩压(分别为r=0.463、r=0.545)、舒张压(分别为r=0.475、r=0.467)及尿蛋白(分别为r=0.302、r=0.342)呈显著正相关(P<0.001),而与终止妊娠孕周(分别为r=-0.281、r=-0.188)、新生儿出生体重(分别为r=-0.299、r=-0.200)及1 min Apgar评分(分别为r=-0.215、r=-0.194)呈显著负相关(P<0.05)。子痫前期组孕妇血清CCL20与IL-17水平呈显著正相关(r=0.615,P<0.001)。血清CCL20及IL-17诊断子痫前期的临界值分别为50.025 pg/ml及48.825 pg/ml,血清CCL20+IL-17联合诊断子痫前期的曲线下面积最大,灵敏度及特异度分别为87.3%及94.4%。结论子痫前期孕妇血清CCL20及IL-17的水平显著高于健康孕妇,且CCL20及IL-17水平与患者的血压、尿蛋白、妊娠结局及新生儿情况均存在显著相关性,两指标联合应用对子痫前期有一定的诊断价值。展开更多
文摘We assessed expression of IL-20 and its receptors in psoriasis,given the recent implication of IL-20 in epidermal hyperplasia.Psoriatic lesional(LS)skin consistently expressed more IL-20 mRNA than nonlesional(NL)skin.Immunoreactivity to IL-20 protein was greater in LS tissue and mainly localized to infiltrating CD68+/CD11c+(myeloid-derived)dermal leukocytes.Because this contrasted with earlier reports of a keratinocyte source,we assessed IL-20 mRNA expression in a variety of cells in vitro,and confirmed a myeloid-derived cellular source(monocytes).Plastic adhesion,activation of β2 integrins,and incubation with tumor necrosis factor-αstimulated expression in these cells.IL-20 receptor(IL-20R)αand IL-20RβmRNA was decreased in LS versus NL skin,which also contrasted with earlier findings.To investigate the relationship between IL-20 and disease activity,we examined psoriasis patients treated with the CD2-targeted agent alefacept.In therapeutic responders,lesional IL-20 mRNA decreased to NL levels,suggesting that CD2+leukocytes may proximally regulate IL-20.Finally,to assess IL-20 function,we used microarrays to screen IL-20-treated keratinocytes,which demonstrated upregulation of disease-related and IFN-γ-induced genes.Hence,IL-20 may influence inflammation through IFN-like effects.Together,these data indicate that IL-20 may be an important effector cytokine in psoriasis,and that its inhibition may represent a potential therapeutic target.
文摘目的探讨血清CC趋化因子配体20(CCL20)及白介素-17(IL-17)水平与子痫前期的相关性及其对子痫前期的诊断效能。方法选择2021年1月至2022年12月陕西省人民医院产科收治的150例子痫前期孕妇为子痫前期组,另选取同期90例健康妊娠妇女为对照组。采用酶联免疫吸附法(ELISA)检测血清CCL20及IL-17的表达水平,采用Pearson相关法分析血清CCL20及IL-17与子痫前期患者临床参数的相关性;采用受试者工作特征曲线(ROC)分析血清CCL20及IL-17对子痫前期的诊断价值。结果子痫前期组孕妇血清CCL20及IL-17水平均显著高于对照组(P<0.001)。子痫前期组孕妇血清CCL20及IL-17的表达均与收缩压(分别为r=0.463、r=0.545)、舒张压(分别为r=0.475、r=0.467)及尿蛋白(分别为r=0.302、r=0.342)呈显著正相关(P<0.001),而与终止妊娠孕周(分别为r=-0.281、r=-0.188)、新生儿出生体重(分别为r=-0.299、r=-0.200)及1 min Apgar评分(分别为r=-0.215、r=-0.194)呈显著负相关(P<0.05)。子痫前期组孕妇血清CCL20与IL-17水平呈显著正相关(r=0.615,P<0.001)。血清CCL20及IL-17诊断子痫前期的临界值分别为50.025 pg/ml及48.825 pg/ml,血清CCL20+IL-17联合诊断子痫前期的曲线下面积最大,灵敏度及特异度分别为87.3%及94.4%。结论子痫前期孕妇血清CCL20及IL-17的水平显著高于健康孕妇,且CCL20及IL-17水平与患者的血压、尿蛋白、妊娠结局及新生儿情况均存在显著相关性,两指标联合应用对子痫前期有一定的诊断价值。
