Objective To investigate the relationship between single nucleotide polymorphisms(SNPs) of the interleukin-4(IL-4) gene and outcome of hepatitis B virus(HBV) infection in a Chinese Han population.Methods Total of 501 ...Objective To investigate the relationship between single nucleotide polymorphisms(SNPs) of the interleukin-4(IL-4) gene and outcome of hepatitis B virus(HBV) infection in a Chinese Han population.Methods Total of 501 patients with chronic hepatitis B virus(HBV) infection and 301 controls with selflimiting HBV infection were studied. Three tag SNPs in the IL-4 gene(rs2227284G/T, rs2243283C/G and rs2243288A/G) were genotyped by the Multiplex snapshot technique. The genotype and allele frequencies were calculated and analyzed.Results The three SNPs showed no significant genotype/allele associations with chronic HBV infection. Overall allele P values were: rs2227284, P = 0.655, odds ratio(OR) [95% confidence interval(CI)] = 1.070(0.793-1.445); rs2243283, P = 0.849, OR(95% CI) = 0.976(0.758-1.257); rs2243288, P = 0.659, OR(95% CI) = 1.060(0.818-1.375). Overall genotype P values were: rs2227284, P = 0.771; rs2243283, P = 0.571; rs2243288, P = 0.902. There were no statistically significant differences between patients with chronic HBV infection and controls. Haplotypes generated by these three SNPs also had no significant differences between the two groups. Conclusions The three tag SNPs of IL-4 were not associated with the outcome of HBV infection in the Han Chinese population.展开更多
基金Supported by Grants from the National Natural Science Foundationof China,No.81072342the National Pre-973 Program Projects,No.2009CB526411
文摘Objective To investigate the relationship between single nucleotide polymorphisms(SNPs) of the interleukin-4(IL-4) gene and outcome of hepatitis B virus(HBV) infection in a Chinese Han population.Methods Total of 501 patients with chronic hepatitis B virus(HBV) infection and 301 controls with selflimiting HBV infection were studied. Three tag SNPs in the IL-4 gene(rs2227284G/T, rs2243283C/G and rs2243288A/G) were genotyped by the Multiplex snapshot technique. The genotype and allele frequencies were calculated and analyzed.Results The three SNPs showed no significant genotype/allele associations with chronic HBV infection. Overall allele P values were: rs2227284, P = 0.655, odds ratio(OR) [95% confidence interval(CI)] = 1.070(0.793-1.445); rs2243283, P = 0.849, OR(95% CI) = 0.976(0.758-1.257); rs2243288, P = 0.659, OR(95% CI) = 1.060(0.818-1.375). Overall genotype P values were: rs2227284, P = 0.771; rs2243283, P = 0.571; rs2243288, P = 0.902. There were no statistically significant differences between patients with chronic HBV infection and controls. Haplotypes generated by these three SNPs also had no significant differences between the two groups. Conclusions The three tag SNPs of IL-4 were not associated with the outcome of HBV infection in the Han Chinese population.
文摘目的探讨新疆地区支气管哮喘(Bronchial Asthma,BA)患者白介素-4(IL-4)基因启动子区甲基化的差异。方法选取2014年1月-2016年10月在新疆医科大学第六附属医院呼吸科收治的BA患者80例为试验组,其中男性42例,女性38例,平均年龄(39.2±3.8)岁;选取同期门诊体检健康人群80例为对照组,其中男性44例,女性36例,平均年龄(40.1±2.6)岁;采用甲基化特异性聚合酶链反应(Methylation Specific PCR,MSP)检测IL-4基因甲基化(碱基C经亚硫酸氢盐修饰后变成U,但已甲基化的C则不再改变,据此设计两对不同的引物PCR扩增可检验其差异)。结果与对照组比较,试验组IL-4基因甲基化率较高,差异有统计学意义(P<0.01),试验组IL-4基因启动子区完全甲基化率为42.5%(34/80),未甲基化率为25.0%(20/80),甲基化和未甲基化共存率为32.5%(26/80);对照组IL-4基因启动子区完全甲基化率为11.3%(9/80),未甲基化率为46.3%(37/80);甲基化和未甲基化共存率为42.5%(34/80)。结论 IL-4基因甲基化程度在BA患者发病中有一定作用。
