This work was supported by a grant from the National Natural Science Foundation of China (No.39730420). This is one of papers of the special issue on gene therapy research (Chin J Cancer Res Vol. 9 No. 4 December, 19...This work was supported by a grant from the National Natural Science Foundation of China (No.39730420). This is one of papers of the special issue on gene therapy research (Chin J Cancer Res Vol. 9 No. 4 December, 1997). Human IL 6 gene was transduced into FBL 3 murine erythroleukemia cells in vitro by calcium phos phate co participation. After selection in the presence of G418, limiting dilution and biological activity assay, G418 resistant clone that secreted the highest level of IL 6 (225.6 U/ml) was selected out of 24 IL 6 secreting clones. The FBL 3 cells secreting the highest level of IL 6 (FBL 3 IL 6) showed decreased growth potential and clono genicity in vitro. Inhibition of cell growth and clone formation was found to be closely related to the level of IL 6 secretion. FBL 3 IL 6 cells grew more slowly than wild type FBL 3 leukemia cells and FBL 3 cells secreting lower level of IL 6 (21.3 U/ml) when inoculated s.c. into C57BL/6 mice. The mice inoculated with FBL 3 IL 6 cells showed prolonged survival period than those inoculated with control leukemia cells. Increased cytotoxic activities of splenic NK and CTL were found in mice inoculated with FBL 3 IL 6 cells. The secretions of IL 2, TNF and GM CSF from murine splenocytes were also found to be greatly elevated after the inoculation of FBL 3 IL 6 leukemia cells. These data suggested that transduction of IL 6 gene into FBL 3 cells magnificently decreased the tumorigenicity and increased the immunogenicity of the leukemia cells, could induce specific and nonspecific antitumor immune responses. IL 6 gene modified leukemia cells might be of great interests to be used as vaccine for the treatment of leukemia.展开更多
目的探讨白细胞介素-6(interleukin 6,IL-6)-572、IL-6-174基因多态性与高血压肾损害及贝那普利治疗反应的相关性。方法入选284例初次诊断高血压病的患者,根据24h尿蛋白排泄率(urinary albumin excretion,UAER)水平分为高血压组(UAER<...目的探讨白细胞介素-6(interleukin 6,IL-6)-572、IL-6-174基因多态性与高血压肾损害及贝那普利治疗反应的相关性。方法入选284例初次诊断高血压病的患者,根据24h尿蛋白排泄率(urinary albumin excretion,UAER)水平分为高血压组(UAER<20μg/min)和高血压肾损害组(UAER≥20μg/min)。检测IL-6水平及IL-6-572、IL-6-174基因多态性。然后用贝那普利干预,观察具备IL-6-572、IL-6-174的不同基因型的患者的治疗反应。结果高血压肾损害组中,IL-6-572的CG基因型、IL-6-174的GG基因型比例最高。贝那普利治疗对具有IL-6-572GG基因型、IL-6-174CC基因型的高血压肾损害患者治疗效果最佳。结论IL-6-572、IL-6-174基因多态性与高血压肾损害及其对贝那普利治疗的反应相关。展开更多
目的:探讨中国人群中IL-6基因启动子中单核苷酸多态性与HBV感染的遗传易感性关联.方法:提取160例HBV感染者及212例健康献血者外周血基因组DNA,用PCR-RFLP方法检测两组人群中G-174C、G-572C和G-597A三个多态性位点的基因型.性别、吸烟、...目的:探讨中国人群中IL-6基因启动子中单核苷酸多态性与HBV感染的遗传易感性关联.方法:提取160例HBV感染者及212例健康献血者外周血基因组DNA,用PCR-RFLP方法检测两组人群中G-174C、G-572C和G-597A三个多态性位点的基因型.性别、吸烟、饮酒以及Hardy-Weinberg等采用Chi-square Test检测,多态性与HBV感染者风险关联及亚组关联的统计学分析采用非条件Logistic回归并同时校正混杂因素.结果:PCR-RFLP检测结果显示:IL-6基因启动子中G-174C和G-597A两个位点在中国人群中不存在多态性,而G-572C处的多态性在人群中普遍存在,其多态性位点等位基因频率分布符合Hardy-Weinberg平衡定律.统计分析表明该位点的多态性在两组人群中有明显的差异(G/C vs G/G,OR=2.65,P<0.05;C/C vs G/G,OR=3.31,P<0.05);亚组分析中表明<30岁的年龄组中差异显著(G/G vs G/C或C/C,OR=16.92,P<0.05).结论:在中国汉族人群中,IL-6基因启动子中-572处的多态性对于乙型肝炎的发生有着显著的易患关系,同时年龄对于这种易感关联有着协同作用.展开更多
文摘This work was supported by a grant from the National Natural Science Foundation of China (No.39730420). This is one of papers of the special issue on gene therapy research (Chin J Cancer Res Vol. 9 No. 4 December, 1997). Human IL 6 gene was transduced into FBL 3 murine erythroleukemia cells in vitro by calcium phos phate co participation. After selection in the presence of G418, limiting dilution and biological activity assay, G418 resistant clone that secreted the highest level of IL 6 (225.6 U/ml) was selected out of 24 IL 6 secreting clones. The FBL 3 cells secreting the highest level of IL 6 (FBL 3 IL 6) showed decreased growth potential and clono genicity in vitro. Inhibition of cell growth and clone formation was found to be closely related to the level of IL 6 secretion. FBL 3 IL 6 cells grew more slowly than wild type FBL 3 leukemia cells and FBL 3 cells secreting lower level of IL 6 (21.3 U/ml) when inoculated s.c. into C57BL/6 mice. The mice inoculated with FBL 3 IL 6 cells showed prolonged survival period than those inoculated with control leukemia cells. Increased cytotoxic activities of splenic NK and CTL were found in mice inoculated with FBL 3 IL 6 cells. The secretions of IL 2, TNF and GM CSF from murine splenocytes were also found to be greatly elevated after the inoculation of FBL 3 IL 6 leukemia cells. These data suggested that transduction of IL 6 gene into FBL 3 cells magnificently decreased the tumorigenicity and increased the immunogenicity of the leukemia cells, could induce specific and nonspecific antitumor immune responses. IL 6 gene modified leukemia cells might be of great interests to be used as vaccine for the treatment of leukemia.
文摘目的探讨白细胞介素-6(interleukin 6,IL-6)-572、IL-6-174基因多态性与高血压肾损害及贝那普利治疗反应的相关性。方法入选284例初次诊断高血压病的患者,根据24h尿蛋白排泄率(urinary albumin excretion,UAER)水平分为高血压组(UAER<20μg/min)和高血压肾损害组(UAER≥20μg/min)。检测IL-6水平及IL-6-572、IL-6-174基因多态性。然后用贝那普利干预,观察具备IL-6-572、IL-6-174的不同基因型的患者的治疗反应。结果高血压肾损害组中,IL-6-572的CG基因型、IL-6-174的GG基因型比例最高。贝那普利治疗对具有IL-6-572GG基因型、IL-6-174CC基因型的高血压肾损害患者治疗效果最佳。结论IL-6-572、IL-6-174基因多态性与高血压肾损害及其对贝那普利治疗的反应相关。
文摘目的:探讨中国人群中IL-6基因启动子中单核苷酸多态性与HBV感染的遗传易感性关联.方法:提取160例HBV感染者及212例健康献血者外周血基因组DNA,用PCR-RFLP方法检测两组人群中G-174C、G-572C和G-597A三个多态性位点的基因型.性别、吸烟、饮酒以及Hardy-Weinberg等采用Chi-square Test检测,多态性与HBV感染者风险关联及亚组关联的统计学分析采用非条件Logistic回归并同时校正混杂因素.结果:PCR-RFLP检测结果显示:IL-6基因启动子中G-174C和G-597A两个位点在中国人群中不存在多态性,而G-572C处的多态性在人群中普遍存在,其多态性位点等位基因频率分布符合Hardy-Weinberg平衡定律.统计分析表明该位点的多态性在两组人群中有明显的差异(G/C vs G/G,OR=2.65,P<0.05;C/C vs G/G,OR=3.31,P<0.05);亚组分析中表明<30岁的年龄组中差异显著(G/G vs G/C或C/C,OR=16.92,P<0.05).结论:在中国汉族人群中,IL-6基因启动子中-572处的多态性对于乙型肝炎的发生有着显著的易患关系,同时年龄对于这种易感关联有着协同作用.
